Restriction map for
pF1KB8399
(Circular) (Six-base) MAPSORT of: pF1KB8399.seq Check: 8559 from: 1 to: 3794
pF1KB8399 3794 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 832 832
Size: 3794
Acc65I G'GTAC_C
Cuts at: 810 810
Size: 3794
AclI AA'CG_TT
Cuts at: 3634 3634
Size: 3794
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1619 2051 2404 3066 1619
Size: 432 353 662 2347
Fragments arranged by size:
2347 662 432 353
AflIII A'CryG_T
Cuts at: 1146 2946 3227 1146
Size: 1800 281 1713
Fragments arranged by size:
1800 1713 281
AleI CACnn'nnGTG
Cuts at: 510 510
Size: 3794
AlwNI CAG_nnn'CTG
Cuts at: 1099 2537 1099
Size: 1438 2356
ApaI G_GGCC'C
Cuts at: 148 148
Size: 3794
ApaLI G'TGCA_C
Cuts at: 2632 2632
Size: 3794
ApoI r'AATT_y
Cuts at: 798 3147 3403 798
Size: 2349 256 1189
Fragments arranged by size:
2349 1189 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3794
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3794
AvaI C'yCGr_G
Cuts at: 140 153 335 467 814 140
Size: 13 182 132 347 3120
Fragments arranged by size:
3120 347 182 132 13
BamHI G'GATC_C
Cuts at: 819 819
Size: 3794
BanI G'GyrC_C
Cuts at: 198 306 340 360 810 1224 1472 1507 198
Size: 108 34 20 450 414 248 35 2485
Fragments arranged by size:
2485 450 414 248 108 35 34 20
BanII G_rGCy'C
Cuts at: 148 466 808 1838 148
Size: 318 342 1030 2104
Fragments arranged by size:
2104 1030 342 318
BbeI G_GCGC'C
Cuts at: 202 364 1228 1476 202
Size: 162 864 248 2520
Fragments arranged by size:
2520 864 248 162
BciVI GTATCCnnnnn_n'
Cuts at: 1692 2748 3794 1692
Size: 1056 1046 1692
Fragments arranged by size:
1692 1056 1046
BclI T'GATC_A
Cuts at: 1314 1314
Size: 3794
BglI GCCn_nnn'nGGC
Cuts at: 478 478
Size: 3794
BglII A'GATC_T
Cuts at: 763 1309 3346 763
Size: 546 2037 1211
Fragments arranged by size:
2037 1211 546
BlpI GC'TnA_GC
Cuts at: 906 906
Size: 3794
Bme1580I G_kGCm'C
Cuts at: 148 215 311 343 544 1419 1512 2636 148
Size: 67 96 32 201 875 93 1124 1306
Fragments arranged by size:
1306 1124 875 201 96 93 67 32
BmrI ACTGGGnnnn_n'
Cuts at: 1006 1087 1422 3566 1006
Size: 81 335 2144 1234
Fragments arranged by size:
2144 1234 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 626 2161 626
Size: 1535 2259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 691 691
Size: 3794
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 970 1849 2563 2861 970
Size: 879 714 298 1903
Fragments arranged by size:
1903 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 165 3498 165
Size: 3333 461
BsaAI yAC'GTr
Cuts at: 1147 1147
Size: 3794
BsaBI GATnn'nnATC
Cuts at: 6 1308 3345 6
Size: 1302 2037 455
Fragments arranged by size:
2037 1302 455
BsaHI Gr'CG_yC
Cuts at: 199 361 1225 1473 2175 3671 199
Size: 162 864 248 702 1496 322
Fragments arranged by size:
1496 864 702 322 248 162
BsaWI w'CCGG_w
Cuts at: 218 771 983 1504 2182 2593 2740 3014 218
Size: 553 212 521 678 411 147 274 998
Fragments arranged by size:
998 678 553 521 411 274 212 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 448 478 448
Size: 30 3764
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 688 688
Size: 3794
BseYI C'CCAG_C
Cuts at: 289 1219 1977 2642 3050 289
Size: 930 758 665 408 1033
Fragments arranged by size:
1033 930 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 481
Size: 3794
BsiEI CG_ry'CG
Cuts at: 104 299 1336 1382 2612 104
Size: 195 1037 46 1230 1286
Fragments arranged by size:
1286 1230 1037 195 46
BsiHKAI G_wGCw'C
Cuts at: 127 808 1586 1776 2636 127
Size: 681 778 190 860 1285
Fragments arranged by size:
1285 860 778 681 190
BsmBI CGTCTCn'nnnn_
Cuts at: 415 437 415
Size: 22 3772
Bsp1286I G_dGCh'C
Cuts at: 127 148 215 311 343 466 544 808 1419
Size: 21 67 96 32 123 78 264 611
Cuts at: 1419 1512 1586 1776 1838 2636 127
Size: 93 74 190 62 798 1285
Fragments arranged by size:
1285 798 611 264 190 123 96 93
78 74 67 62 32 21
BspEI T'CCGG_A
Cuts at: 771 771
Size: 3794
BspHI T'CATG_A
Cuts at: 2226 2226
Size: 3794
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 1058 1360 1741 844
Size: 214 302 381 2897
Fragments arranged by size:
2897 381 302 214
BsrBI CCG'CTC
Cuts at: 50 137 254 2086 2140 3624 3789 50
Size: 87 117 1832 54 1484 165 55
Fragments arranged by size:
1832 1484 165 117 87 55 54
BsrDI GCAATG_nn'
Cuts at: 414 1706 414
Size: 1292 2502
BsrFI r'CCGG_y
Cuts at: 343 1792 1973 343
Size: 1449 181 2164
Fragments arranged by size:
2164 1449 181
BssSI C'ACGA_G
Cuts at: 744 2065 2773 744
Size: 1321 708 1765
Fragments arranged by size:
1765 1321 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1171 1171
Size: 3794
BstBI TT'CG_AA
Cuts at: 2155 2155
Size: 3794
BstEII G'GTnAC_C
Cuts at: 412 433 412
Size: 21 3773
BstXI CCAn_nnnn'nTGG
Cuts at: 409 561 409
Size: 152 3642
BstYI r'GATC_y
Cuts at: 1 763 819 1286 1309 1644 1890 2196 2208
Size: 762 56 467 23 335 246 306 12
Cuts at: 2208 2294 3346 1
Size: 86 1052 449
Fragments arranged by size:
1052 762 467 449 335 306 246 86
56 23 12
BtgI C'CryG_G
Cuts at: 107 236 402 107
Size: 129 166 3499
Fragments arranged by size:
3499 166 129
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 486 1744 1925 2088 486
Size: 1258 181 163 2192
Fragments arranged by size:
2192 1258 181 163
BtsI GCAGTG_nn'
Cuts at: 1037 1037
Size: 3794
DraI TTT'AAA
Cuts at: 793 793
Size: 3794
DrdI GACnn_nn'nnGTC
Cuts at: 1500 2844 1500
Size: 1344 2450
EaeI y'GGCC_r
Cuts at: 296 1379 1553 1944 1971 3316 296
Size: 1083 174 391 27 1345 774
Fragments arranged by size:
1345 1083 774 391 174 27
EagI C'GGCC_G
Cuts at: 296 1379 296
Size: 1083 2711
EarI CTCTTCn'nnn_
Cuts at: 1817 2027 1817
Size: 210 3584
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 386 496 2002 2744 2890 3604 386
Size: 110 1506 742 146 714 576
Fragments arranged by size:
1506 742 714 576 146 110
EcoICRI GAG'CTC
Cuts at: 806 806
Size: 3794
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 691 1619 2051 2404 3066 691
Size: 928 432 353 662 1419
Fragments arranged by size:
1419 928 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 144 145 933 144
Size: 1 788 3005
Fragments arranged by size:
3005 788 1
EcoRI G'AATT_C
Cuts at: 798 798
Size: 3794
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1813 1813
Size: 3794
FspI TGC'GCA
Cuts at: 247 1575 247
Size: 1328 2466
HaeII r_GCGC'y
Cuts at: 179 202 364 1228 1476 2706 3477 179
Size: 23 162 864 248 1230 771 496
Fragments arranged by size:
1230 864 771 496 248 162 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1636 1666 1668 1698 1636
Size: 30 2 30 3732
Fragments arranged by size:
3732 30 30 2
HincII GTy'rAC
Cuts at: 833 833
Size: 3794
KasI G'GCGC_C
Cuts at: 198 360 1224 1472 198
Size: 162 864 248 2520
Fragments arranged by size:
2520 864 248 162
KpnI G_GTAC'C
Cuts at: 814 814
Size: 3794
MluI A'CGCG_T
Cuts at: 3227 3227
Size: 3794
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 410 2553 2737 410
Size: 2143 184 1467
Fragments arranged by size:
2143 1467 184
MscI TGG'CCA
Cuts at: 1555 1555
Size: 3794
MslI CAynn'nnrTG
Cuts at: 280 407 510 1783 1910 280
Size: 127 103 1273 127 2164
Fragments arranged by size:
2164 1273 127 127 103
MspA1I CmG'CkG
Cuts at: 905 1219 1579 2363 2608 905
Size: 314 360 784 245 2091
Fragments arranged by size:
2091 784 360 314 245
NaeI GCC'GGC
Cuts at: 1975 1975
Size: 3794
NarI GG'CG_CC
Cuts at: 199 361 1225 1473 199
Size: 162 864 248 2520
Fragments arranged by size:
2520 864 248 162
NcoI C'CATG_G
Cuts at: 107 236 402 107
Size: 129 166 3499
Fragments arranged by size:
3499 166 129
NgoMIV G'CCGG_C
Cuts at: 1973 1973
Size: 3794
NspI r_CATG'y
Cuts at: 847 1347 2950 847
Size: 500 1603 1691
Fragments arranged by size:
1691 1603 500
PciI A'CATG_T
Cuts at: 2946 2946
Size: 3794
PmeI GTTT'AAAC
Cuts at: 793 793
Size: 3794
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2240 2272 2240
Size: 32 3762
PsiI TTA'TAA
Cuts at: 592 592
Size: 3794
PspOMI G'GGCC_C
Cuts at: 144 144
Size: 3794
PstI C_TGCA'G
Cuts at: 686 841 1526 686
Size: 155 685 2954
Fragments arranged by size:
2954 685 155
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3794
PvuII CAG'CTG
Cuts at: 1219 1579 1219
Size: 360 3434
RsrII CG'GwC_CG
Cuts at: 1989 1989
Size: 3794
SacI G_AGCT'C
Cuts at: 808 808
Size: 3794
SalI G'TCGA_C
Cuts at: 831 831
Size: 3794
SapI GCTCTTCn'nnn_
Cuts at: 1817 2027 1817
Size: 210 3584
SbfI CC_TGCA'GG
Cuts at: 841 841
Size: 3794
ScaI AGT'ACT
Cuts at: 3311 3311
Size: 3794
SfcI C'TryA_G
Cuts at: 33 682 837 1522 2490 2681 33
Size: 649 155 685 968 191 1146
Fragments arranged by size:
1146 968 685 649 191 155
SfoI GGC'GCC
Cuts at: 200 362 1226 1474 200
Size: 162 864 248 2520
Fragments arranged by size:
2520 864 248 162
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3794
SgrAI Cr'CCGG_yG
Cuts at: 343 343
Size: 3794
SmaI CCC'GGG
Cuts at: 142 155 337 816 142
Size: 13 182 479 3120
Fragments arranged by size:
3120 479 182 13
SmlI C'TyrA_G
Cuts at: 949 1864 2578 2840 949
Size: 915 714 262 1903
Fragments arranged by size:
1903 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3794
SphI G_CATG'C
Cuts at: 847 1347 847
Size: 500 3294
StyI C'CwwG_G
Cuts at: 107 236 402 928 1282 107
Size: 129 166 526 354 2619
Fragments arranged by size:
2619 526 354 166 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 246 522 1377 1510 1969 2178 246
Size: 276 855 133 459 209 1862
Fragments arranged by size:
1862 855 459 276 209 133
TatI w'GTAC_w
Cuts at: 3309 3309
Size: 3794
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3311 3343 3311
Size: 32 3762
Tth111I GACn'n_nGTC
Cuts at: 1591 1591
Size: 3794
XbaI T'CTAG_A
Cuts at: 825 825
Size: 3794
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1289 1289
Size: 3794
XmaI C'CCGG_G
Cuts at: 140 153 335 814 140
Size: 13 182 479 3120
Fragments arranged by size:
3120 479 182 13
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsiWI BsmI BsrGI BssHII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PspXI PsrI
SacII SanDI SexAI SfiI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI