Restriction map for
pF1KB8403
(Circular) (Six-base) MAPSORT of: pF1KB8403.seq Check: 5826 from: 1 to: 5258
pF1KB8403 5258 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AatII G_ACGT'C
Cuts at: 611 611
Size: 5258
AccI GT'mk_AC
Cuts at: 578 2296 578
Size: 1718 3540
Acc65I G'GTAC_C
Cuts at: 2274 2274
Size: 5258
AclI AA'CG_TT
Cuts at: 5098 5098
Size: 5258
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1067 1625 3083 3515 3868 4530 1067
Size: 558 1458 432 353 662 1795
Fragments arranged by size:
1795 1458 662 558 432 353
AflIII A'CryG_T
Cuts at: 201 1206 1874 2610 4410 4691 201
Size: 1005 668 736 1800 281 768
Fragments arranged by size:
1800 1005 768 736 668 281
AlwNI CAG_nnn'CTG
Cuts at: 534 2122 2563 4001 534
Size: 1588 441 1438 1791
Fragments arranged by size:
1791 1588 1438 441
ApaLI G'TGCA_C
Cuts at: 4096 4096
Size: 5258
ApoI r'AATT_y
Cuts at: 451 489 908 929 1554 1582 1684 1974 2262
Size: 38 419 21 625 28 102 290 288
Cuts at: 2262 4611 4867 451
Size: 2349 256 842
Fragments arranged by size:
2349 842 625 419 290 288 256 102
38 28 21
AseI AT'TA_AT
Cuts at: 20 1138 1577 20
Size: 1118 439 3701
Fragments arranged by size:
3701 1118 439
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5258
AvaI C'yCGr_G
Cuts at: 2278 2278
Size: 5258
BamHI G'GATC_C
Cuts at: 2283 2283
Size: 5258
BanI G'GyrC_C
Cuts at: 2274 2688 2936 2971 2274
Size: 414 248 35 4561
Fragments arranged by size:
4561 414 248 35
BanII G_rGCy'C
Cuts at: 686 2272 3302 686
Size: 1586 1030 2642
Fragments arranged by size:
2642 1586 1030
BbeI G_GCGC'C
Cuts at: 2692 2940 2692
Size: 248 5010
BbsI GAAGACnn'nnnn_
Cuts at: 404 404
Size: 5258
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 122 156 1156 1190 122
Size: 34 1000 34 4190
Fragments arranged by size:
4190 1000 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 958 1824 3156 4212 5258 958
Size: 866 1332 1056 1046 958
Fragments arranged by size:
1332 1056 1046 958 866
BclI T'GATC_A
Cuts at: 156 2778 156
Size: 2622 2636
BglII A'GATC_T
Cuts at: 2773 4810 2773
Size: 2037 3221
BlpI GC'TnA_GC
Cuts at: 941 2370 941
Size: 1429 3829
Bme1580I G_kGCm'C
Cuts at: 1195 2883 2976 4100 1195
Size: 1688 93 1124 2353
Fragments arranged by size:
2353 1688 1124 93
BmgBI CAC'GTC
Cuts at: 123 123
Size: 5258
BmrI ACTGGGnnnn_n'
Cuts at: 874 2470 2551 2886 5030 874
Size: 1596 81 335 2144 1102
Fragments arranged by size:
2144 1596 1102 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 993 3625 993
Size: 2632 2626
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 719 2022 719
Size: 1303 3955
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1940 2147 2434 3313 4027 4325 1940
Size: 207 287 879 714 298 2873
Fragments arranged by size:
2873 879 714 298 287 207
BsaI GGTCTCn'nnnn_
Cuts at: 4962 4962
Size: 5258
BsaAI yAC'GTr
Cuts at: 2611 2611
Size: 5258
BsaBI GATnn'nnATC
Cuts at: 6 2772 4809 6
Size: 2766 2037 455
Fragments arranged by size:
2766 2037 455
BsaHI Gr'CG_yC
Cuts at: 608 2689 2937 3639 5135 608
Size: 2081 248 702 1496 731
Fragments arranged by size:
2081 1496 731 702 248
BsaWI w'CCGG_w
Cuts at: 2447 2968 3646 4057 4204 4478 2447
Size: 521 678 411 147 274 3227
Fragments arranged by size:
3227 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1745 1745
Size: 5258
BseYI C'CCAG_C
Cuts at: 2683 3441 4106 4514 2683
Size: 758 665 408 3427
Fragments arranged by size:
3427 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2800 2846 4076 104
Size: 2696 46 1230 1286
Fragments arranged by size:
2696 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2272 3050 3240 4100 2272
Size: 778 190 860 3430
Fragments arranged by size:
3430 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 686 1195 2272 2883 2976 3050 3240 3302 4100
Size: 509 1077 611 93 74 190 62 798
Cuts at: 4100 686
Size: 1844
Fragments arranged by size:
1844 1077 798 611 509 190 93 74
62
BspHI T'CATG_A
Cuts at: 153 2088 3690 153
Size: 1935 1602 1721
Fragments arranged by size:
1935 1721 1602
BspMI ACCTGCnnnn'nnnn_
Cuts at: 310 608 2308 2522 2824 3205 310
Size: 298 1700 214 302 381 2363
Fragments arranged by size:
2363 1700 381 302 298 214
BsrBI CCG'CTC
Cuts at: 50 3550 3604 5088 5253 50
Size: 3500 54 1484 165 55
Fragments arranged by size:
3500 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3170 3170
Size: 5258
BsrFI r'CCGG_y
Cuts at: 3256 3437 3256
Size: 181 5077
BsrGI T'GTAC_A
Cuts at: 1039 1039
Size: 5258
BssSI C'ACGA_G
Cuts at: 3529 4237 3529
Size: 708 4550
BstAPI GCAn_nnn'nTGC
Cuts at: 2635 2635
Size: 5258
BstBI TT'CG_AA
Cuts at: 1281 3619 1281
Size: 2338 2920
BstEII G'GTnAC_C
Cuts at: 297 297
Size: 5258
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 5258
BstYI r'GATC_y
Cuts at: 1 207 498 634 2283 2750 2773 3108 3354
Size: 206 291 136 1649 467 23 335 246
Cuts at: 3354 3660 3672 3758 4810 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1649 1052 467 449 335 306 291 246
206 136 86 23 12
BstZ17I GTA'TAC
Cuts at: 579 579
Size: 5258
BtgI C'CryG_G
Cuts at: 107 107
Size: 5258
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3208 3389 3552 3208
Size: 181 163 4914
Fragments arranged by size:
4914 181 163
BtsI GCAGTG_nn'
Cuts at: 2501 2501
Size: 5258
DraI TTT'AAA
Cuts at: 747 1032 1094 1437 2257 747
Size: 285 62 343 820 3748
Fragments arranged by size:
3748 820 343 285 62
DrdI GACnn_nn'nnGTC
Cuts at: 1621 2964 4308 1621
Size: 1343 1344 2571
Fragments arranged by size:
2571 1344 1343
EaeI y'GGCC_r
Cuts at: 2843 3017 3408 3435 4780 2843
Size: 174 391 27 1345 3321
Fragments arranged by size:
3321 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2843 2843
Size: 5258
EarI CTCTTCn'nnn_
Cuts at: 514 2212 3281 3491 514
Size: 1698 1069 210 2281
Fragments arranged by size:
2281 1698 1069 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 133 3466 4208 4354 5068 133
Size: 3333 742 146 714 323
Fragments arranged by size:
3333 742 714 323 146
EcoICRI GAG'CTC
Cuts at: 2270 2270
Size: 5258
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 719 1067 1625 2022 3083 3515 3868 4530 719
Size: 348 558 397 1061 432 353 662 1447
Fragments arranged by size:
1447 1061 662 558 432 397 353 348
EcoO109I rG'GnC_Cy
Cuts at: 1942 2397 1942
Size: 455 4803
EcoRI G'AATT_C
Cuts at: 1582 2262 1582
Size: 680 4578
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1096 1678 3277 1096
Size: 582 1599 3077
Fragments arranged by size:
3077 1599 582
FspI TGC'GCA
Cuts at: 3039 3039
Size: 5258
HaeII r_GCGC'y
Cuts at: 2692 2940 4170 4941 2692
Size: 248 1230 771 3009
Fragments arranged by size:
3009 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 231 263 1127 1159 1780 1812 3100 3130 3132
Size: 32 864 32 621 32 1288 30 2
Cuts at: 3132 3162 231
Size: 30 2327
Fragments arranged by size:
2327 1288 864 621 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 787 1614 2297 787
Size: 827 683 3748
Fragments arranged by size:
3748 827 683
KasI G'GCGC_C
Cuts at: 2688 2936 2688
Size: 248 5010
KpnI G_GTAC'C
Cuts at: 2278 2278
Size: 5258
MluI A'CGCG_T
Cuts at: 4691 4691
Size: 5258
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 612 4017 4201 612
Size: 3405 184 1669
Fragments arranged by size:
3405 1669 184
MscI TGG'CCA
Cuts at: 3019 3019
Size: 5258
MslI CAynn'nnrTG
Cuts at: 1453 3247 3374 1453
Size: 1794 127 3337
Fragments arranged by size:
3337 1794 127
MspA1I CmG'CkG
Cuts at: 534 2369 2683 3043 3827 4072 534
Size: 1835 314 360 784 245 1720
Fragments arranged by size:
1835 1720 784 360 314 245
NaeI GCC'GGC
Cuts at: 3439 3439
Size: 5258
NarI GG'CG_CC
Cuts at: 2689 2937 2689
Size: 248 5010
NcoI C'CATG_G
Cuts at: 107 107
Size: 5258
NdeI CA'TA_TG
Cuts at: 1454 1454
Size: 5258
NgoMIV G'CCGG_C
Cuts at: 3437 3437
Size: 5258
NspI r_CATG'y
Cuts at: 205 1210 1723 1878 2311 2811 4414 205
Size: 1005 513 155 433 500 1603 1049
Fragments arranged by size:
1603 1049 1005 513 500 433 155
PciI A'CATG_T
Cuts at: 201 1206 1874 4410 201
Size: 1005 668 2536 1049
Fragments arranged by size:
2536 1049 1005 668
PmeI GTTT'AAAC
Cuts at: 2257 2257
Size: 5258
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3704 3736 3704
Size: 32 5226
PpuMI rG'GwC_Cy
Cuts at: 1942 1942
Size: 5258
PsiI TTA'TAA
Cuts at: 428 428
Size: 5258
PstI C_TGCA'G
Cuts at: 2305 2990 2305
Size: 685 4573
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5258
PvuII CAG'CTG
Cuts at: 534 2683 3043 534
Size: 2149 360 2749
Fragments arranged by size:
2749 2149 360
RsrII CG'GwC_CG
Cuts at: 3453 3453
Size: 5258
SacI G_AGCT'C
Cuts at: 2272 2272
Size: 5258
SalI G'TCGA_C
Cuts at: 2295 2295
Size: 5258
SapI GCTCTTCn'nnn_
Cuts at: 3281 3491 3281
Size: 210 5048
SbfI CC_TGCA'GG
Cuts at: 2305 2305
Size: 5258
ScaI AGT'ACT
Cuts at: 4775 4775
Size: 5258
SexAI A'CCwGG_T
Cuts at: 825 825
Size: 5258
SfcI C'TryA_G
Cuts at: 33 411 526 2051 2301 2986 3954 4145 33
Size: 378 115 1525 250 685 968 191 1146
Fragments arranged by size:
1525 1146 968 685 378 250 191 115
SfoI GGC'GCC
Cuts at: 2690 2938 2690
Size: 248 5010
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5258
SmaI CCC'GGG
Cuts at: 2280 2280
Size: 5258
SmlI C'TyrA_G
Cuts at: 1955 2162 2413 3328 4042 4304 1955
Size: 207 251 915 714 262 2909
Fragments arranged by size:
2909 915 714 262 251 207
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5258
SphI G_CATG'C
Cuts at: 2311 2811 2311
Size: 500 4758
SspI AAT'ATT
Cuts at: 289 289
Size: 5258
StyI C'CwwG_G
Cuts at: 107 2392 2746 107
Size: 2285 354 2619
Fragments arranged by size:
2619 2285 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 918 2841 2974 3433 3642 918
Size: 1923 133 459 209 2534
Fragments arranged by size:
2534 1923 459 209 133
TatI w'GTAC_w
Cuts at: 198 1039 1519 2190 4773 198
Size: 841 480 671 2583 683
Fragments arranged by size:
2583 841 683 671 480
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1686 1718 4775 4807 1686
Size: 32 3057 32 2137
Fragments arranged by size:
3057 2137 32 32
Tth111I GACn'n_nGTC
Cuts at: 564 3055 564
Size: 2491 2767
XbaI T'CTAG_A
Cuts at: 1726 2289 1726
Size: 563 4695
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2753 2753
Size: 5258
XmaI C'CCGG_G
Cuts at: 2278 2278
Size: 5258
XmnI GAAnn'nnTTC
Cuts at: 1463 1685 1463
Size: 222 5036
ZraI GAC'GTC
Cuts at: 609 609
Size: 5258
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PmeI PpiI PpuMI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmtI
Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BspEI BssHII
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PshAI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI