Restriction map for
pF1KB8406
(Circular) (Six-base) MAPSORT of: pF1KB8406.seq Check: 5928 from: 1 to: 3896
pF1KB8406 3896 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 934 934
Size: 3896
Acc65I G'GTAC_C
Cuts at: 912 912
Size: 3896
AclI AA'CG_TT
Cuts at: 3736 3736
Size: 3896
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 546 714 798 1721 2153 2506 3168 546
Size: 168 84 923 432 353 662 1274
Fragments arranged by size:
1274 923 662 432 353 168 84
AflIII A'CryG_T
Cuts at: 1248 3048 3329 1248
Size: 1800 281 1815
Fragments arranged by size:
1815 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 581 1201 2639 581
Size: 620 1438 1838
Fragments arranged by size:
1838 1438 620
ApaLI G'TGCA_C
Cuts at: 2734 2734
Size: 3896
ApoI r'AATT_y
Cuts at: 136 176 416 900 3249 3505 136
Size: 40 240 484 2349 256 527
Fragments arranged by size:
2349 527 484 256 240 40
AseI AT'TA_AT
Cuts at: 20 20
Size: 3896
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3896
AvaI C'yCGr_G
Cuts at: 916 916
Size: 3896
BamHI G'GATC_C
Cuts at: 921 921
Size: 3896
BanI G'GyrC_C
Cuts at: 912 1326 1574 1609 912
Size: 414 248 35 3199
Fragments arranged by size:
3199 414 248 35
BanII G_rGCy'C
Cuts at: 406 910 1940 406
Size: 504 1030 2362
Fragments arranged by size:
2362 1030 504
BbeI G_GCGC'C
Cuts at: 1330 1578 1330
Size: 248 3648
BciVI GTATCCnnnnn_n'
Cuts at: 1794 2850 3896 1794
Size: 1056 1046 1794
Fragments arranged by size:
1794 1056 1046
BclI T'GATC_A
Cuts at: 1416 1416
Size: 3896
BfrBI ATG'CAT
Cuts at: 168 504 588 168
Size: 336 84 3476
Fragments arranged by size:
3476 336 84
BglII A'GATC_T
Cuts at: 156 1411 3448 156
Size: 1255 2037 604
Fragments arranged by size:
2037 1255 604
BlpI GC'TnA_GC
Cuts at: 1008 1008
Size: 3896
Bme1580I G_kGCm'C
Cuts at: 124 1521 1614 2738 124
Size: 1397 93 1124 1282
Fragments arranged by size:
1397 1282 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1108 1189 1524 3668 1108
Size: 81 335 2144 1336
Fragments arranged by size:
2144 1336 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2263 2263
Size: 3896
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 558 710 558
Size: 152 3744
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1072 1951 2665 2963 1072
Size: 879 714 298 2005
Fragments arranged by size:
2005 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 254 3600 254
Size: 3346 550
BsaAI yAC'GTr
Cuts at: 1249 1249
Size: 3896
BsaBI GATnn'nnATC
Cuts at: 6 1410 3447 6
Size: 1404 2037 455
Fragments arranged by size:
2037 1404 455
BsaHI Gr'CG_yC
Cuts at: 1327 1575 2277 3773 1327
Size: 248 702 1496 1450
Fragments arranged by size:
1496 1450 702 248
BsaWI w'CCGG_w
Cuts at: 520 1085 1606 2284 2695 2842 3116 520
Size: 565 521 678 411 147 274 1300
Fragments arranged by size:
1300 678 565 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 263 293 263
Size: 30 3866
BseYI C'CCAG_C
Cuts at: 1321 2079 2744 3152 1321
Size: 758 665 408 2065
Fragments arranged by size:
2065 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1438 1484 2714 104
Size: 1334 46 1230 1286
Fragments arranged by size:
1334 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 406 910 1688 1878 2738 406
Size: 504 778 190 860 1564
Fragments arranged by size:
1564 860 778 504 190
Bsp1286I G_dGCh'C
Cuts at: 124 406 910 1521 1614 1688 1878 1940 2738
Size: 282 504 611 93 74 190 62 798
Cuts at: 2738 124
Size: 1282
Fragments arranged by size:
1282 798 611 504 282 190 93 74
62
BspHI T'CATG_A
Cuts at: 2328 2328
Size: 3896
BspMI ACCTGCnnnn'nnnn_
Cuts at: 946 1160 1462 1843 946
Size: 214 302 381 2999
Fragments arranged by size:
2999 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2188 2242 3726 3891 50
Size: 2138 54 1484 165 55
Fragments arranged by size:
2138 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1808 1808
Size: 3896
BsrFI r'CCGG_y
Cuts at: 1894 2075 1894
Size: 181 3715
BssSI C'ACGA_G
Cuts at: 2167 2875 2167
Size: 708 3188
BstAPI GCAn_nnn'nTGC
Cuts at: 1273 1273
Size: 3896
BstBI TT'CG_AA
Cuts at: 2257 2257
Size: 3896
BstYI r'GATC_y
Cuts at: 1 156 921 1388 1411 1746 1992 2298 2310
Size: 155 765 467 23 335 246 306 12
Cuts at: 2310 2396 3448 1
Size: 86 1052 449
Fragments arranged by size:
1052 765 467 449 335 306 246 155
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1846 2027 2190 1846
Size: 181 163 3552
Fragments arranged by size:
3552 181 163
BtsI GCAGTG_nn'
Cuts at: 1139 1139
Size: 3896
DraI TTT'AAA
Cuts at: 537 895 537
Size: 358 3538
DraIII CAC_nnn'GTG
Cuts at: 354 354
Size: 3896
DrdI GACnn_nn'nnGTC
Cuts at: 1602 2946 1602
Size: 1344 2552
EaeI y'GGCC_r
Cuts at: 1481 1655 2046 2073 3418 1481
Size: 174 391 27 1345 1959
Fragments arranged by size:
1959 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1481 1481
Size: 3896
EarI CTCTTCn'nnn_
Cuts at: 539 1919 2129 539
Size: 1380 210 2306
Fragments arranged by size:
2306 1380 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2104 2846 2992 3706 2104
Size: 742 146 714 2294
Fragments arranged by size:
2294 742 714 146
EcoICRI GAG'CTC
Cuts at: 404 908 404
Size: 504 3392
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 546 558 710 714 798 1721 2153 2506 3168
Size: 12 152 4 84 923 432 353 662
Cuts at: 3168 546
Size: 1274
Fragments arranged by size:
1274 923 662 432 353 152 84 12
4
EcoO109I rG'GnC_Cy
Cuts at: 1035 1035
Size: 3896
EcoRI G'AATT_C
Cuts at: 900 900
Size: 3896
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 243 1915 243
Size: 1672 2224
FspI TGC'GCA
Cuts at: 1677 1677
Size: 3896
HaeII r_GCGC'y
Cuts at: 1330 1578 2808 3579 1330
Size: 248 1230 771 1647
Fragments arranged by size:
1647 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 141 173 263 295 393 425 855 887 1738
Size: 32 90 32 98 32 430 32 851
Cuts at: 1738 1768 1770 1800 141
Size: 30 2 30 2237
Fragments arranged by size:
2237 851 430 98 90 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 935 935
Size: 3896
KasI G'GCGC_C
Cuts at: 1326 1574 1326
Size: 248 3648
KpnI G_GTAC'C
Cuts at: 916 916
Size: 3896
MfeI C'AATT_G
Cuts at: 396 396
Size: 3896
MluI A'CGCG_T
Cuts at: 3329 3329
Size: 3896
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2655 2839 2655
Size: 184 3712
MscI TGG'CCA
Cuts at: 1657 1657
Size: 3896
MslI CAynn'nnrTG
Cuts at: 203 437 1885 2012 203
Size: 234 1448 127 2087
Fragments arranged by size:
2087 1448 234 127
MspA1I CmG'CkG
Cuts at: 187 1007 1321 1681 2465 2710 187
Size: 820 314 360 784 245 1373
Fragments arranged by size:
1373 820 784 360 314 245
NaeI GCC'GGC
Cuts at: 2077 2077
Size: 3896
NarI GG'CG_CC
Cuts at: 1327 1575 1327
Size: 248 3648
NdeI CA'TA_TG
Cuts at: 422 422
Size: 3896
NgoMIV G'CCGG_C
Cuts at: 2075 2075
Size: 3896
NsiI A_TGCA'T
Cuts at: 170 506 590 170
Size: 336 84 3476
Fragments arranged by size:
3476 336 84
NspI r_CATG'y
Cuts at: 504 588 949 1449 3052 504
Size: 84 361 500 1603 1348
Fragments arranged by size:
1603 1348 500 361 84
PciI A'CATG_T
Cuts at: 3048 3048
Size: 3896
PflMI CCAn_nnn'nTGG
Cuts at: 354 354
Size: 3896
PmeI GTTT'AAAC
Cuts at: 895 895
Size: 3896
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2342 2374 2342
Size: 32 3864
PstI C_TGCA'G
Cuts at: 943 1628 943
Size: 685 3211
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3896
PvuII CAG'CTG
Cuts at: 187 1321 1681 187
Size: 1134 360 2402
Fragments arranged by size:
2402 1134 360
RsrII CG'GwC_CG
Cuts at: 2091 2091
Size: 3896
SacI G_AGCT'C
Cuts at: 406 910 406
Size: 504 3392
SalI G'TCGA_C
Cuts at: 933 933
Size: 3896
SapI GCTCTTCn'nnn_
Cuts at: 1919 2129 1919
Size: 210 3686
SbfI CC_TGCA'GG
Cuts at: 943 943
Size: 3896
ScaI AGT'ACT
Cuts at: 3413 3413
Size: 3896
SfcI C'TryA_G
Cuts at: 33 456 702 939 1624 2592 2783 33
Size: 423 246 237 685 968 191 1146
Fragments arranged by size:
1146 968 685 423 246 237 191
SfoI GGC'GCC
Cuts at: 1328 1576 1328
Size: 248 3648
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3896
SmaI CCC'GGG
Cuts at: 918 918
Size: 3896
SmlI C'TyrA_G
Cuts at: 1051 1966 2680 2942 1051
Size: 915 714 262 2005
Fragments arranged by size:
2005 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3896
SphI G_CATG'C
Cuts at: 504 588 949 1449 504
Size: 84 361 500 2951
Fragments arranged by size:
2951 500 361 84
StuI AGG'CCT
Cuts at: 559 727 811 559
Size: 168 84 3644
Fragments arranged by size:
3644 168 84
StyI C'CwwG_G
Cuts at: 1030 1384 1030
Size: 354 3542
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1479 1612 2071 2280 1479
Size: 133 459 209 3095
Fragments arranged by size:
3095 459 209 133
TatI w'GTAC_w
Cuts at: 3411 3411
Size: 3896
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3413 3445 3413
Size: 32 3864
Tth111I GACn'n_nGTC
Cuts at: 1693 1693
Size: 3896
XbaI T'CTAG_A
Cuts at: 927 927
Size: 3896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1391 1391
Size: 3896
XmaI C'CCGG_G
Cuts at: 916 916
Size: 3896
XmnI GAAnn'nnTTC
Cuts at: 559 643 727 784 559
Size: 84 84 57 3671
Fragments arranged by size:
3671 84 84 57
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI
BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NsiI NspI PciI PflMI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI
BglI BmgBI BmtI Bpu10I BseRI BsgI BsiWI BsmI
BsmBI BspEI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
BtgI ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI NcoI NheI NotI NruI PacI PasI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI