Restriction map for
pF1KB8410
(Circular) (Six-base) MAPSORT of: pF1KB8410.seq Check: 6469 from: 1 to: 4061
pF1KB8410 4061 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 108 108
Size: 4061
AccI GT'mk_AC
Cuts at: 1099 1099
Size: 4061
Acc65I G'GTAC_C
Cuts at: 1077 1077
Size: 4061
AclI AA'CG_TT
Cuts at: 3901 3901
Size: 4061
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1886 2318 2671 3333 1886
Size: 432 353 662 2614
Fragments arranged by size:
2614 662 432 353
AflIII A'CryG_T
Cuts at: 504 1413 3213 3494 504
Size: 909 1800 281 1071
Fragments arranged by size:
1800 1071 909 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 416 416
Size: 4061
AlwNI CAG_nnn'CTG
Cuts at: 295 1366 2804 295
Size: 1071 1438 1552
Fragments arranged by size:
1552 1438 1071
ApaLI G'TGCA_C
Cuts at: 2899 2899
Size: 4061
ApoI r'AATT_y
Cuts at: 259 380 1065 3414 3670 259
Size: 121 685 2349 256 650
Fragments arranged by size:
2349 685 650 256 121
AseI AT'TA_AT
Cuts at: 20 20
Size: 4061
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4061
AvaI C'yCGr_G
Cuts at: 148 1081 148
Size: 933 3128
BamHI G'GATC_C
Cuts at: 854 1086 854
Size: 232 3829
BanI G'GyrC_C
Cuts at: 1077 1491 1739 1774 1077
Size: 414 248 35 3364
Fragments arranged by size:
3364 414 248 35
BanII G_rGCy'C
Cuts at: 688 1075 2105 688
Size: 387 1030 2644
Fragments arranged by size:
2644 1030 387
BbeI G_GCGC'C
Cuts at: 1495 1743 1495
Size: 248 3813
BciVI GTATCCnnnnn_n'
Cuts at: 1959 3015 4061 1959
Size: 1056 1046 1959
Fragments arranged by size:
1959 1056 1046
BclI T'GATC_A
Cuts at: 1581 1581
Size: 4061
BglII A'GATC_T
Cuts at: 1576 3613 1576
Size: 2037 2024
BlpI GC'TnA_GC
Cuts at: 1173 1173
Size: 4061
Bme1580I G_kGCm'C
Cuts at: 1686 1779 2903 1686
Size: 93 1124 2844
Fragments arranged by size:
2844 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1273 1354 1689 3833 1273
Size: 81 335 2144 1501
Fragments arranged by size:
2144 1501 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2428 2428
Size: 4061
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 371 672 702 1000 371
Size: 301 30 298 3432
Fragments arranged by size:
3432 301 298 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1237 2116 2830 3128 1237
Size: 879 714 298 2170
Fragments arranged by size:
2170 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3765 3765
Size: 4061
BsaAI yAC'GTr
Cuts at: 1414 1414
Size: 4061
BsaBI GATnn'nnATC
Cuts at: 6 1575 3612 6
Size: 1569 2037 455
Fragments arranged by size:
2037 1569 455
BsaHI Gr'CG_yC
Cuts at: 1492 1740 2442 3938 1492
Size: 248 702 1496 1615
Fragments arranged by size:
1615 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1250 1771 2449 2860 3007 3281 1250
Size: 521 678 411 147 274 2030
Fragments arranged by size:
2030 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 847 847
Size: 4061
BseYI C'CCAG_C
Cuts at: 1486 2244 2909 3317 1486
Size: 758 665 408 2230
Fragments arranged by size:
2230 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 145 154 945 136
Size: 9 9 791 3252
Fragments arranged by size:
3252 791 9 9
BsiEI CG_ry'CG
Cuts at: 104 1603 1649 2879 104
Size: 1499 46 1230 1286
Fragments arranged by size:
1499 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 193 688 1075 1853 2043 2903 193
Size: 495 387 778 190 860 1351
Fragments arranged by size:
1351 860 778 495 387 190
Bsp1286I G_dGCh'C
Cuts at: 193 688 1075 1686 1779 1853 2043 2105 2903
Size: 495 387 611 93 74 190 62 798
Cuts at: 2903 193
Size: 1351
Fragments arranged by size:
1351 798 611 495 387 190 93 74
62
BspHI T'CATG_A
Cuts at: 2493 2493
Size: 4061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 108 1111 1325 1627 2008 108
Size: 1003 214 302 381 2161
Fragments arranged by size:
2161 1003 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2353 2407 3891 4056 50
Size: 2303 54 1484 165 55
Fragments arranged by size:
2303 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1973 1973
Size: 4061
BsrFI r'CCGG_y
Cuts at: 2059 2240 2059
Size: 181 3880
BssSI C'ACGA_G
Cuts at: 671 2332 3040 671
Size: 1661 708 1692
Fragments arranged by size:
1692 1661 708
BstAPI GCAn_nnn'nTGC
Cuts at: 123 132 1438 123
Size: 9 1306 2746
Fragments arranged by size:
2746 1306 9
BstBI TT'CG_AA
Cuts at: 2422 2422
Size: 4061
BstYI r'GATC_y
Cuts at: 1 818 854 890 1086 1553 1576 1911 2157
Size: 817 36 36 196 467 23 335 246
Cuts at: 2157 2463 2475 2561 3613 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 817 467 449 335 306 246 196
86 36 36 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4061
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2011 2192 2355 2011
Size: 181 163 3717
Fragments arranged by size:
3717 181 163
BtsI GCAGTG_nn'
Cuts at: 1304 1304
Size: 4061
DraI TTT'AAA
Cuts at: 1060 1060
Size: 4061
DrdI GACnn_nn'nnGTC
Cuts at: 1767 3111 1767
Size: 1344 2717
EaeI y'GGCC_r
Cuts at: 603 1646 1820 2211 2238 3583 603
Size: 1043 174 391 27 1345 1081
Fragments arranged by size:
1345 1081 1043 391 174 27
EagI C'GGCC_G
Cuts at: 1646 1646
Size: 4061
EarI CTCTTCn'nnn_
Cuts at: 716 1019 2084 2294 716
Size: 303 1065 210 2483
Fragments arranged by size:
2483 1065 303 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2269 3011 3157 3871 2269
Size: 742 146 714 2459
Fragments arranged by size:
2459 742 714 146
EcoICRI GAG'CTC
Cuts at: 686 1073 686
Size: 387 3674
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 371 672 702 1000 1886 2318 2671 3333 371
Size: 301 30 298 886 432 353 662 1099
Fragments arranged by size:
1099 886 662 432 353 301 298 30
EcoNI CCTnn'n_nnAGG
Cuts at: 826 826
Size: 4061
EcoO109I rG'GnC_Cy
Cuts at: 1200 1200
Size: 4061
EcoRI G'AATT_C
Cuts at: 1065 1065
Size: 4061
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 808 2080 808
Size: 1272 2789
FspI TGC'GCA
Cuts at: 286 1842 286
Size: 1556 2505
FspAI rTGC'GCAy
Cuts at: 286 286
Size: 4061
HaeII r_GCGC'y
Cuts at: 1495 1743 2973 3744 1495
Size: 248 1230 771 1812
Fragments arranged by size:
1812 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 382 414 528 560 1903 1933 1935 1965 382
Size: 32 114 32 1343 30 2 30 2478
Fragments arranged by size:
2478 1343 114 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1100 1100
Size: 4061
KasI G'GCGC_C
Cuts at: 1491 1739 1491
Size: 248 3813
KpnI G_GTAC'C
Cuts at: 1081 1081
Size: 4061
MluI A'CGCG_T
Cuts at: 3494 3494
Size: 4061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 947 2820 3004 947
Size: 1873 184 2004
Fragments arranged by size:
2004 1873 184
MscI TGG'CCA
Cuts at: 1822 1822
Size: 4061
MslI CAynn'nnrTG
Cuts at: 112 509 2050 2177 112
Size: 397 1541 127 1996
Fragments arranged by size:
1996 1541 397 127
MspA1I CmG'CkG
Cuts at: 396 947 1172 1486 1846 2630 2875 396
Size: 551 225 314 360 784 245 1582
Fragments arranged by size:
1582 784 551 360 314 245 225
NaeI GCC'GGC
Cuts at: 2242 2242
Size: 4061
NarI GG'CG_CC
Cuts at: 1492 1740 1492
Size: 248 3813
NcoI C'CATG_G
Cuts at: 107 107
Size: 4061
NgoMIV G'CCGG_C
Cuts at: 2240 2240
Size: 4061
NspI r_CATG'y
Cuts at: 508 1114 1614 3217 508
Size: 606 500 1603 1352
Fragments arranged by size:
1603 1352 606 500
PciI A'CATG_T
Cuts at: 504 3213 504
Size: 2709 1352
PflMI CCAn_nnn'nTGG
Cuts at: 540 540
Size: 4061
PmeI GTTT'AAAC
Cuts at: 1060 1060
Size: 4061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2507 2539 2507
Size: 32 4029
PstI C_TGCA'G
Cuts at: 405 1108 1793 405
Size: 703 685 2673
Fragments arranged by size:
2673 703 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4061
PvuII CAG'CTG
Cuts at: 396 1486 1846 396
Size: 1090 360 2611
Fragments arranged by size:
2611 1090 360
RsrII CG'GwC_CG
Cuts at: 2256 2256
Size: 4061
SacI G_AGCT'C
Cuts at: 688 1075 688
Size: 387 3674
SalI G'TCGA_C
Cuts at: 1098 1098
Size: 4061
SapI GCTCTTCn'nnn_
Cuts at: 1019 2084 2294 1019
Size: 1065 210 2786
Fragments arranged by size:
2786 1065 210
SbfI CC_TGCA'GG
Cuts at: 1108 1108
Size: 4061
ScaI AGT'ACT
Cuts at: 3578 3578
Size: 4061
SfcI C'TryA_G
Cuts at: 33 401 958 1104 1789 2757 2948 33
Size: 368 557 146 685 968 191 1146
Fragments arranged by size:
1146 968 685 557 368 191 146
SfoI GGC'GCC
Cuts at: 1493 1741 1493
Size: 248 3813
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4061
SmaI CCC'GGG
Cuts at: 1083 1083
Size: 4061
SmlI C'TyrA_G
Cuts at: 148 1216 2131 2845 3107 148
Size: 1068 915 714 262 1102
Fragments arranged by size:
1102 1068 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4061
SphI G_CATG'C
Cuts at: 1114 1614 1114
Size: 500 3561
StyI C'CwwG_G
Cuts at: 107 1195 1549 107
Size: 1088 354 2619
Fragments arranged by size:
2619 1088 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 386 1644 1777 2236 2445 386
Size: 1258 133 459 209 2002
Fragments arranged by size:
2002 1258 459 209 133
TatI w'GTAC_w
Cuts at: 3576 3576
Size: 4061
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3578 3610 3578
Size: 32 4029
Tth111I GACn'n_nGTC
Cuts at: 1858 1858
Size: 4061
XbaI T'CTAG_A
Cuts at: 1050 1092 1050
Size: 42 4019
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 456 677 1556 456
Size: 221 879 2961
Fragments arranged by size:
2961 879 221
XhoI C'TCGA_G
Cuts at: 148 148
Size: 4061
XmaI C'CCGG_G
Cuts at: 1081 1081
Size: 4061
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BspEI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XmnI
ZraI