Restriction map for
pF1KB8411
(Circular) (Six-base) MAPSORT of: pF1KB8411.seq Check: 9343 from: 1 to: 5711
pF1KB8411 5711 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 2749 2749
Size: 5711
Acc65I G'GTAC_C
Cuts at: 2727 2727
Size: 5711
AclI AA'CG_TT
Cuts at: 5551 5551
Size: 5711
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 725 2183 2339 2381 3536 3968 4321 4983 725
Size: 1458 156 42 1155 432 353 662 1453
Fragments arranged by size:
1458 1453 1155 662 432 353 156 42
AfeI AGC'GCT
Cuts at: 1213 1213
Size: 5711
AflIII A'CryG_T
Cuts at: 1801 3063 4863 5144 1801
Size: 1262 1800 281 2368
Fragments arranged by size:
2368 1800 1262 281
AleI CACnn'nnGTG
Cuts at: 2138 2138
Size: 5711
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 950 950
Size: 5711
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 520 552 520
Size: 32 5679
AlwNI CAG_nnn'CTG
Cuts at: 648 1359 2481 3016 4454 648
Size: 711 1122 535 1438 1905
Fragments arranged by size:
1905 1438 1122 711 535
ApaI G_GGCC'C
Cuts at: 200 752 1287 200
Size: 552 535 4624
Fragments arranged by size:
4624 552 535
ApaLI G'TGCA_C
Cuts at: 4549 4549
Size: 5711
ApoI r'AATT_y
Cuts at: 334 367 739 1669 2715 5064 5320 334
Size: 33 372 930 1046 2349 256 725
Fragments arranged by size:
2349 1046 930 725 372 256 33
AseI AT'TA_AT
Cuts at: 20 2228 20
Size: 2208 3503
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5711
AvaI C'yCGr_G
Cuts at: 2460 2731 2460
Size: 271 5440
BamHI G'GATC_C
Cuts at: 2736 2736
Size: 5711
BanI G'GyrC_C
Cuts at: 1919 2194 2727 3141 3389 3424 1919
Size: 275 533 414 248 35 4206
Fragments arranged by size:
4206 533 414 275 248 35
BanII G_rGCy'C
Cuts at: 200 752 905 956 1287 1298 1778 2274 2725
Size: 552 153 51 331 11 480 496 451
Cuts at: 2725 3755 200
Size: 1030 2156
Fragments arranged by size:
2156 1030 552 496 480 451 331 153
51 11
BbeI G_GCGC'C
Cuts at: 3145 3393 3145
Size: 248 5463
BbsI GAAGACnn'nnnn_
Cuts at: 548 620 842 1109 1592 548
Size: 72 222 267 483 4667
Fragments arranged by size:
4667 483 267 222 72
BbvCI CC'TCA_GC
Cuts at: 260 806 260
Size: 546 5165
BciVI GTATCCnnnnn_n'
Cuts at: 3609 4665 5711 3609
Size: 1056 1046 3609
Fragments arranged by size:
3609 1056 1046
BclI T'GATC_A
Cuts at: 597 3231 597
Size: 2634 3077
BglI GCCn_nnn'nGGC
Cuts at: 1747 1747
Size: 5711
BglII A'GATC_T
Cuts at: 3226 5263 3226
Size: 2037 3674
BlpI GC'TnA_GC
Cuts at: 1777 2823 1777
Size: 1046 4665
Bme1580I G_kGCm'C
Cuts at: 200 752 1287 1924 2199 2603 3336 3429 4553
Size: 552 535 637 275 404 733 93 1124
Cuts at: 4553 200
Size: 1358
Fragments arranged by size:
1358 1124 733 637 552 535 404 275
93
BmgBI CAC'GTC
Cuts at: 1513 1513
Size: 5711
BmrI ACTGGGnnnn_n'
Cuts at: 724 1118 2923 3004 3339 5483 724
Size: 394 1805 81 335 2144 952
Fragments arranged by size:
2144 1805 952 394 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4078 4078
Size: 5711
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1846 2440 2677 1846
Size: 594 237 4880
Fragments arranged by size:
4880 594 237
Bpu10I CC'TnA_GC
Cuts at: 260 806 260
Size: 546 5165
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 881 2887 3766 4480 4778 881
Size: 2006 879 714 298 1814
Fragments arranged by size:
2006 1814 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5415 5415
Size: 5711
BsaAI yAC'GTr
Cuts at: 1802 2598 3064 1802
Size: 796 466 4449
Fragments arranged by size:
4449 796 466
BsaBI GATnn'nnATC
Cuts at: 6 3225 5262 6
Size: 3219 2037 455
Fragments arranged by size:
3219 2037 455
BsaHI Gr'CG_yC
Cuts at: 3142 3390 4092 5588 3142
Size: 248 702 1496 3265
Fragments arranged by size:
3265 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2900 3421 4099 4510 4657 4931 2900
Size: 521 678 411 147 274 3680
Fragments arranged by size:
3680 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 240 270 1502 1532 240
Size: 30 1232 30 4419
Fragments arranged by size:
4419 1232 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 207 248 794 1228 1696 1894 1897 207
Size: 41 546 434 468 198 3 4021
Fragments arranged by size:
4021 546 468 434 198 41 3
BseYI C'CCAG_C
Cuts at: 252 773 2586 2687 3136 3894 4559 4967 252
Size: 521 1813 101 449 758 665 408 996
Fragments arranged by size:
1813 996 758 665 521 449 408 101
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1273 1782 2461 1273
Size: 509 679 4523
Fragments arranged by size:
4523 679 509
BsiEI CG_ry'CG
Cuts at: 104 3253 3299 4529 104
Size: 3149 46 1230 1286
Fragments arranged by size:
3149 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 956 1262 1298 1518 1778 1838 2663 2725 3503
Size: 306 36 220 260 60 825 62 778
Cuts at: 3503 3693 4553 956
Size: 190 860 2114
Fragments arranged by size:
2114 860 825 778 306 260 220 190
62 60 36
BsiWI C'GTAC_G
Cuts at: 2594 2594
Size: 5711
Bsp1286I G_dGCh'C
Cuts at: 200 752 905 956 1262 1287 1298 1518 1778
Size: 552 153 51 306 25 11 220 260
Cuts at: 1778 1838 1924 2199 2274 2603 2663 2725 3336
Size: 60 86 275 75 329 60 62 611
Cuts at: 3336 3429 3503 3693 3755 4553 200
Size: 93 74 190 62 798 1358
Fragments arranged by size:
1358 798 611 552 329 306 275 260
220 190 153 93 86 75 74 62
62 60 60 51 25 11
BspHI T'CATG_A
Cuts at: 4143 4143
Size: 5711
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2489 2675 2761 2975 3277 3658 2489
Size: 186 86 214 302 381 4542
Fragments arranged by size:
4542 381 302 214 186 86
BsrBI CCG'CTC
Cuts at: 50 2261 4003 4057 5541 5706 50
Size: 2211 1742 54 1484 165 55
Fragments arranged by size:
2211 1742 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3623 3623
Size: 5711
BsrFI r'CCGG_y
Cuts at: 3709 3890 3709
Size: 181 5530
BssSI C'ACGA_G
Cuts at: 1291 3982 4690 1291
Size: 2691 708 2312
Fragments arranged by size:
2691 2312 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2183 2481 2667 3088 2183
Size: 298 186 421 4806
Fragments arranged by size:
4806 421 298 186
BstBI TT'CG_AA
Cuts at: 4072 4072
Size: 5711
BstEII G'GTnAC_C
Cuts at: 246 246
Size: 5711
BstXI CCAn_nnnn'nTGG
Cuts at: 781 1824 2140 781
Size: 1043 316 4352
Fragments arranged by size:
4352 1043 316
BstYI r'GATC_y
Cuts at: 1 342 1759 2736 3203 3226 3561 3807 4113
Size: 341 1417 977 467 23 335 246 306
Cuts at: 4113 4125 4211 5263 1
Size: 12 86 1052 449
Fragments arranged by size:
1417 1052 977 467 449 341 335 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 689 689
Size: 5711
BtgI C'CryG_G
Cuts at: 107 2692 107
Size: 2585 3126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2683 3661 3842 4005 2683
Size: 978 181 163 4389
Fragments arranged by size:
4389 978 181 163
BtsI GCAGTG_nn'
Cuts at: 156 1699 1986 2954 156
Size: 1543 287 968 2913
Fragments arranged by size:
2913 1543 968 287
DraI TTT'AAA
Cuts at: 2710 2710
Size: 5711
DrdI GACnn_nn'nnGTC
Cuts at: 1912 3417 4761 1912
Size: 1505 1344 2862
Fragments arranged by size:
2862 1505 1344
EaeI y'GGCC_r
Cuts at: 384 1139 1244 1635 3296 3470 3861 3888 5233
Size: 755 105 391 1661 174 391 27 1345
Cuts at: 5233 384
Size: 862
Fragments arranged by size:
1661 1345 862 755 391 391 174 105
27
EagI C'GGCC_G
Cuts at: 3296 3296
Size: 5711
EarI CTCTTCn'nnn_
Cuts at: 107 125 269 804 857 1384 1957 2048 2357
Size: 18 144 535 53 527 573 91 309
Cuts at: 2357 3734 3944 107
Size: 1377 210 1874
Fragments arranged by size:
1874 1377 573 535 527 309 210 144
91 53 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3919 4661 4807 5521 3919
Size: 742 146 714 4109
Fragments arranged by size:
4109 742 714 146
EcoICRI GAG'CTC
Cuts at: 954 1296 1776 2723 954
Size: 342 480 947 3942
Fragments arranged by size:
3942 947 480 342
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 725 1846 2183 2339 2381 2440 2677 3536 3968
Size: 1121 337 156 42 59 237 859 432
Cuts at: 3968 4321 4983 725
Size: 353 662 1453
Fragments arranged by size:
1453 1121 859 662 432 353 337 237
156 59 42
EcoO109I rG'GnC_Cy
Cuts at: 279 748 793 1284 1499 2454 2578 2850 279
Size: 469 45 491 215 955 124 272 3140
Fragments arranged by size:
3140 955 491 469 272 215 124 45
EcoRI G'AATT_C
Cuts at: 367 739 2715 367
Size: 372 1976 3363
Fragments arranged by size:
3363 1976 372
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 508 530 3730 508
Size: 22 3200 2489
Fragments arranged by size:
3200 2489 22
FspI TGC'GCA
Cuts at: 1698 3492 1698
Size: 1794 3917
HaeII r_GCGC'y
Cuts at: 1215 3145 3393 4623 5394 1215
Size: 1930 248 1230 771 1532
Fragments arranged by size:
1930 1532 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 547 579 1502 1534 1635 1667 2285 2317 3553
Size: 32 923 32 101 32 618 32 1236
Cuts at: 3553 3583 3585 3615 547
Size: 30 2 30 2643
Fragments arranged by size:
2643 1236 923 618 101 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 447 2625 2750 447
Size: 2178 125 3408
Fragments arranged by size:
3408 2178 125
HindIII A'AGCT_T
Cuts at: 2332 2332
Size: 5711
KasI G'GCGC_C
Cuts at: 3141 3389 3141
Size: 248 5463
KpnI G_GTAC'C
Cuts at: 2731 2731
Size: 5711
MluI A'CGCG_T
Cuts at: 5144 5144
Size: 5711
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1751 1928 4470 4654 1751
Size: 177 2542 184 2808
Fragments arranged by size:
2808 2542 184 177
MscI TGG'CCA
Cuts at: 386 1141 1637 3472 386
Size: 755 496 1835 2625
Fragments arranged by size:
2625 1835 755 496
MslI CAynn'nnrTG
Cuts at: 2138 2647 3700 3827 2138
Size: 509 1053 127 4022
Fragments arranged by size:
4022 1053 509 127
MspA1I CmG'CkG
Cuts at: 583 1528 1641 1781 1796 1973 2189 2418 2694
Size: 945 113 140 15 177 216 229 276
Cuts at: 2694 2822 3136 3496 4280 4525 583
Size: 128 314 360 784 245 1769
Fragments arranged by size:
1769 945 784 360 314 276 245 229
216 177 140 128 113 15
NaeI GCC'GGC
Cuts at: 3892 3892
Size: 5711
NarI GG'CG_CC
Cuts at: 3142 3390 3142
Size: 248 5463
NcoI C'CATG_G
Cuts at: 107 107
Size: 5711
NgoMIV G'CCGG_C
Cuts at: 3890 3890
Size: 5711
NspI r_CATG'y
Cuts at: 2181 2652 2764 3264 4867 2181
Size: 471 112 500 1603 3025
Fragments arranged by size:
3025 1603 500 471 112
PasI CC'CwG_GG
Cuts at: 745 745
Size: 5711
PciI A'CATG_T
Cuts at: 4863 4863
Size: 5711
PfoI T'CCnGG_A
Cuts at: 354 354
Size: 5711
PmeI GTTT'AAAC
Cuts at: 2710 2710
Size: 5711
PmlI CAC'GTG
Cuts at: 1802 1802
Size: 5711
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1531 1563 4157 4189 1531
Size: 32 2594 32 3053
Fragments arranged by size:
3053 2594 32 32
PpuMI rG'GwC_Cy
Cuts at: 279 1499 279
Size: 1220 4491
PshAI GACnn'nnGTC
Cuts at: 2636 2636
Size: 5711
PsiI TTA'TAA
Cuts at: 1117 1117
Size: 5711
PspOMI G'GGCC_C
Cuts at: 196 748 1283 196
Size: 552 535 4624
Fragments arranged by size:
4624 552 535
PstI C_TGCA'G
Cuts at: 939 2758 3443 939
Size: 1819 685 3207
Fragments arranged by size:
3207 1819 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5711
PvuII CAG'CTG
Cuts at: 583 1528 1641 1781 1796 1973 3136 3496 583
Size: 945 113 140 15 177 1163 360 2798
Fragments arranged by size:
2798 1163 945 360 177 140 113 15
RsrII CG'GwC_CG
Cuts at: 3906 3906
Size: 5711
SacI G_AGCT'C
Cuts at: 956 1298 1778 2725 956
Size: 342 480 947 3942
Fragments arranged by size:
3942 947 480 342
SacII CC_GC'GG
Cuts at: 2695 2695
Size: 5711
SalI G'TCGA_C
Cuts at: 2748 2748
Size: 5711
SapI GCTCTTCn'nnn_
Cuts at: 857 2048 3734 3944 857
Size: 1191 1686 210 2624
Fragments arranged by size:
2624 1686 1191 210
SbfI CC_TGCA'GG
Cuts at: 2758 2758
Size: 5711
ScaI AGT'ACT
Cuts at: 1861 5228 1861
Size: 3367 2344
SexAI A'CCwGG_T
Cuts at: 677 677
Size: 5711
SfcI C'TryA_G
Cuts at: 33 935 1603 1929 2004 2754 3439 4407 4598
Size: 902 668 326 75 750 685 968 191
Cuts at: 4598 33
Size: 1146
Fragments arranged by size:
1146 968 902 750 685 668 326 191
75
SfoI GGC'GCC
Cuts at: 3143 3391 3143
Size: 248 5463
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5711
SmaI CCC'GGG
Cuts at: 2733 2733
Size: 5711
SmlI C'TyrA_G
Cuts at: 896 2866 3781 4495 4757 896
Size: 1970 915 714 262 1850
Fragments arranged by size:
1970 1850 915 714 262
SpeI A'CTAG_T
Cuts at: 67 966 67
Size: 899 4812
SphI G_CATG'C
Cuts at: 2764 3264 2764
Size: 500 5211
StuI AGG'CCT
Cuts at: 1327 1327
Size: 5711
StyI C'CwwG_G
Cuts at: 107 760 2845 3199 107
Size: 653 2085 354 2619
Fragments arranged by size:
2619 2085 653 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 429 1523 2323 2534 3294 3427 3886 4095 429
Size: 1094 800 211 760 133 459 209 2045
Fragments arranged by size:
2045 1094 800 760 459 211 209 133
TatI w'GTAC_w
Cuts at: 1056 1859 5226 1056
Size: 803 3367 1541
Fragments arranged by size:
3367 1541 803
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 240 272 1264 1296 5228 5260 240
Size: 32 992 32 3932 32 691
Fragments arranged by size:
3932 992 691 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 416 442 3508 416
Size: 26 3066 2619
Fragments arranged by size:
3066 2619 26
XbaI T'CTAG_A
Cuts at: 2742 2742
Size: 5711
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3206 3206
Size: 5711
XmaI C'CCGG_G
Cuts at: 2731 2731
Size: 5711
XmnI GAAnn'nnTTC
Cuts at: 233 1025 1966 2395 233
Size: 792 941 429 3549
Fragments arranged by size:
3549 941 792 429
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AscI AvrII BaeI
BcgI BfrBI BmtI BsmI BsmBI BspEI BsrGI BssHII
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PspXI PsrI SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI