Restriction map for
pF1KB8418
(Circular) (Six-base) MAPSORT of: pF1KB8418.seq Check: 768 from: 1 to: 5540
pF1KB8418 5540 bp
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
AccI GT'mk_AC
Cuts at: 2578 2578
Size: 5540
Acc65I G'GTAC_C
Cuts at: 2556 2556
Size: 5540
AclI AA'CG_TT
Cuts at: 5380 5380
Size: 5540
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2504 3365 3797 4150 4812 2504
Size: 861 432 353 662 3232
Fragments arranged by size:
3232 861 662 432 353
AflIII A'CryG_T
Cuts at: 799 1135 2892 4692 4973 799
Size: 336 1757 1800 281 1366
Fragments arranged by size:
1800 1757 1366 336 281
AlwNI CAG_nnn'CTG
Cuts at: 2845 4283 2845
Size: 1438 4102
ApaLI G'TGCA_C
Cuts at: 484 4378 484
Size: 3894 1646
ApoI r'AATT_y
Cuts at: 142 186 577 585 705 958 1025 1109 1210
Size: 44 391 8 120 253 67 84 101
Cuts at: 1210 1277 1445 1529 1613 1865 1882 1949 2285
Size: 67 168 84 84 252 17 67 336
Cuts at: 2285 2369 2453 2544 4893 5149 142
Size: 84 84 91 2349 256 533
Fragments arranged by size:
2349 533 391 336 256 253 252 168
120 101 91 84 84 84 84 84
67 67 67 44 17 8
AseI AT'TA_AT
Cuts at: 20 776 20
Size: 756 4784
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5540
AvaI C'yCGr_G
Cuts at: 2560 2560
Size: 5540
BamHI G'GATC_C
Cuts at: 2565 2565
Size: 5540
BanI G'GyrC_C
Cuts at: 2556 2970 3218 3253 2556
Size: 414 248 35 4843
Fragments arranged by size:
4843 414 248 35
BanII G_rGCy'C
Cuts at: 2554 3584 2554
Size: 1030 4510
BbeI G_GCGC'C
Cuts at: 2974 3222 2974
Size: 248 5292
BbsI GAAGACnn'nnnn_
Cuts at: 222 222
Size: 5540
BciVI GTATCCnnnnn_n'
Cuts at: 3438 4494 5540 3438
Size: 1056 1046 3438
Fragments arranged by size:
3438 1056 1046
BclI T'GATC_A
Cuts at: 263 3060 263
Size: 2797 2743
BglII A'GATC_T
Cuts at: 3055 5092 3055
Size: 2037 3503
BlpI GC'TnA_GC
Cuts at: 2652 2652
Size: 5540
Bme1580I G_kGCm'C
Cuts at: 488 3165 3258 4382 488
Size: 2677 93 1124 1646
Fragments arranged by size:
2677 1646 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 328 2752 2833 3168 5312 328
Size: 2424 81 335 2144 556
Fragments arranged by size:
2424 2144 556 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2488 3907 2488
Size: 1419 4121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 295 803 971 1055 1139 1391 1475 1727
Size: 123 508 168 84 84 252 84 252
Cuts at: 1727 1811 1895 1979 2147 2231 2315 2483 172
Size: 84 84 84 168 84 84 168 3229
Fragments arranged by size:
3229 508 252 252 168 168 168 123
84 84 84 84 84 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2716 3595 4309 4607 2716
Size: 879 714 298 3649
Fragments arranged by size:
3649 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5244 5244
Size: 5540
BsaAI yAC'GTr
Cuts at: 2893 2893
Size: 5540
BsaBI GATnn'nnATC
Cuts at: 6 3054 5091 6
Size: 3048 2037 455
Fragments arranged by size:
3048 2037 455
BsaHI Gr'CG_yC
Cuts at: 2971 3219 3921 5417 2971
Size: 248 702 1496 3094
Fragments arranged by size:
3094 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2729 3250 3928 4339 4486 4760 2729
Size: 521 678 411 147 274 3509
Fragments arranged by size:
3509 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 169 321 506 2059 169
Size: 152 185 1553 3650
Fragments arranged by size:
3650 1553 185 152
BseYI C'CCAG_C
Cuts at: 2965 3723 4388 4796 2965
Size: 758 665 408 3709
Fragments arranged by size:
3709 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 3082 3128 4358 104
Size: 2978 46 1230 1286
Fragments arranged by size:
2978 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 488 2554 3332 3522 4382 488
Size: 2066 778 190 860 1646
Fragments arranged by size:
2066 1646 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 488 2554 3165 3258 3332 3522 3584 4382 488
Size: 2066 611 93 74 190 62 798 1646
Fragments arranged by size:
2066 1646 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 3972 3972
Size: 5540
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2590 2804 3106 3487 2590
Size: 214 302 381 4643
Fragments arranged by size:
4643 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3832 3886 5370 5535 50
Size: 3782 54 1484 165 55
Fragments arranged by size:
3782 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 169 3452 169
Size: 3283 2257
BsrFI r'CCGG_y
Cuts at: 1247 1583 3538 3719 1247
Size: 336 1955 181 3068
Fragments arranged by size:
3068 1955 336 181
BsrGI T'GTAC_A
Cuts at: 441 1222 441
Size: 781 4759
BssSI C'ACGA_G
Cuts at: 3811 4519 3811
Size: 708 4832
BstAPI GCAn_nnn'nTGC
Cuts at: 2917 2917
Size: 5540
BstBI TT'CG_AA
Cuts at: 3901 3901
Size: 5540
BstYI r'GATC_y
Cuts at: 1 387 2565 3032 3055 3390 3636 3942 3954
Size: 386 2178 467 23 335 246 306 12
Cuts at: 3954 4040 5092 1
Size: 86 1052 449
Fragments arranged by size:
2178 1052 467 449 386 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 5540
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 159 3490 3671 3834 159
Size: 3331 181 163 1865
Fragments arranged by size:
3331 1865 181 163
BtsI GCAGTG_nn'
Cuts at: 2783 2783
Size: 5540
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 624 659 624
Size: 35 5505
DraI TTT'AAA
Cuts at: 449 2169 2539 449
Size: 1720 370 3450
Fragments arranged by size:
3450 1720 370
DrdI GACnn_nn'nnGTC
Cuts at: 3246 4590 3246
Size: 1344 4196
EaeI y'GGCC_r
Cuts at: 134 367 3125 3299 3690 3717 5062 134
Size: 233 2758 174 391 27 1345 612
Fragments arranged by size:
2758 1345 612 391 233 174 27
EagI C'GGCC_G
Cuts at: 3125 3125
Size: 5540
EarI CTCTTCn'nnn_
Cuts at: 3563 3773 3563
Size: 210 5330
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3748 4490 4636 5350 3748
Size: 742 146 714 3938
Fragments arranged by size:
3938 742 714 146
EcoICRI GAG'CTC
Cuts at: 2552 2552
Size: 5540
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 295 803 971 1055 1139 1391 1475 1727
Size: 123 508 168 84 84 252 84 252
Cuts at: 1727 1811 1895 1979 2147 2231 2315 2483 2504
Size: 84 84 84 168 84 84 168 21
Cuts at: 2504 3365 3797 4150 4812 172
Size: 861 432 353 662 900
Fragments arranged by size:
900 861 662 508 432 353 252 252
168 168 168 123 84 84 84 84
84 84 84 84 21
EcoO109I rG'GnC_Cy
Cuts at: 2679 2679
Size: 5540
EcoRI G'AATT_C
Cuts at: 142 1109 1445 1529 2369 2544 142
Size: 967 336 84 840 175 3138
Fragments arranged by size:
3138 967 840 336 175 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3559 3559
Size: 5540
FspI TGC'GCA
Cuts at: 3321 3321
Size: 5540
HaeII r_GCGC'y
Cuts at: 2974 3222 4452 5223 2974
Size: 248 1230 771 3291
Fragments arranged by size:
3291 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 255 287 860 892 2496 2528 3382 3412 3414
Size: 32 573 32 1604 32 854 30 2
Cuts at: 3414 3444 255
Size: 30 2351
Fragments arranged by size:
2351 1604 854 573 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1361 2579 1361
Size: 1218 4322
HindIII A'AGCT_T
Cuts at: 734 902 986 1238 1322 1342 1574 1658 1678
Size: 168 84 252 84 20 232 84 20
Cuts at: 1678 1742 1826 1910 1994 2162 2246 2414 734
Size: 64 84 84 84 168 84 168 3860
Fragments arranged by size:
3860 252 232 168 168 168 84 84
84 84 84 84 84 64 20 20
HpaI GTT'AAC
Cuts at: 1361 1361
Size: 5540
KasI G'GCGC_C
Cuts at: 2970 3218 2970
Size: 248 5292
KpnI G_GTAC'C
Cuts at: 2560 2560
Size: 5540
MluI A'CGCG_T
Cuts at: 4973 4973
Size: 5540
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4299 4483 4299
Size: 184 5356
MscI TGG'CCA
Cuts at: 136 369 3301 136
Size: 233 2932 2375
Fragments arranged by size:
2932 2375 233
MslI CAynn'nnrTG
Cuts at: 437 464 3529 3656 437
Size: 27 3065 127 2321
Fragments arranged by size:
3065 2321 127 27
MspA1I CmG'CkG
Cuts at: 2651 2965 3325 4109 4354 2651
Size: 314 360 784 245 3837
Fragments arranged by size:
3837 784 360 314 245
NaeI GCC'GGC
Cuts at: 3721 3721
Size: 5540
NarI GG'CG_CC
Cuts at: 2971 3219 2971
Size: 248 5292
NcoI C'CATG_G
Cuts at: 107 107
Size: 5540
NgoMIV G'CCGG_C
Cuts at: 3719 3719
Size: 5540
NspI r_CATG'y
Cuts at: 663 803 1139 2593 3093 4696 663
Size: 140 336 1454 500 1603 1507
Fragments arranged by size:
1603 1507 1454 500 336 140
PciI A'CATG_T
Cuts at: 799 1135 4692 799
Size: 336 3557 1647
Fragments arranged by size:
3557 1647 336
PfoI T'CCnGG_A
Cuts at: 1032 1032
Size: 5540
PmeI GTTT'AAAC
Cuts at: 2539 2539
Size: 5540
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3986 4018 3986
Size: 32 5508
PsiI TTA'TAA
Cuts at: 500 500
Size: 5540
PstI C_TGCA'G
Cuts at: 1206 1542 2587 3272 1206
Size: 336 1045 685 3474
Fragments arranged by size:
3474 1045 685 336
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5540
PvuII CAG'CTG
Cuts at: 2965 3325 2965
Size: 360 5180
RsrII CG'GwC_CG
Cuts at: 3735 3735
Size: 5540
SacI G_AGCT'C
Cuts at: 2554 2554
Size: 5540
SalI G'TCGA_C
Cuts at: 2577 2577
Size: 5540
SapI GCTCTTCn'nnn_
Cuts at: 3563 3773 3563
Size: 210 5330
SbfI CC_TGCA'GG
Cuts at: 2587 2587
Size: 5540
ScaI AGT'ACT
Cuts at: 5057 5057
Size: 5540
SexAI A'CCwGG_T
Cuts at: 224 224
Size: 5540
SfcI C'TryA_G
Cuts at: 33 216 1202 1538 2583 3268 4236 4427 33
Size: 183 986 336 1045 685 968 191 1146
Fragments arranged by size:
1146 1045 986 968 685 336 191 183
SfoI GGC'GCC
Cuts at: 2972 3220 2972
Size: 248 5292
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5540
SmaI CCC'GGG
Cuts at: 2562 2562
Size: 5540
SmlI C'TyrA_G
Cuts at: 2695 3610 4324 4586 2695
Size: 915 714 262 3649
Fragments arranged by size:
3649 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5540
SphI G_CATG'C
Cuts at: 663 2593 3093 663
Size: 1930 500 3110
Fragments arranged by size:
3110 1930 500
SspI AAT'ATT
Cuts at: 542 2428 542
Size: 1886 3654
StyI C'CwwG_G
Cuts at: 107 292 2674 3028 107
Size: 185 2382 354 2619
Fragments arranged by size:
2619 2382 354 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3123 3256 3715 3924 3123
Size: 133 459 209 4739
Fragments arranged by size:
4739 459 209 133
TatI w'GTAC_w
Cuts at: 441 1222 5055 441
Size: 781 3833 926
Fragments arranged by size:
3833 926 781
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5057 5089 5057
Size: 32 5508
Tth111I GACn'n_nGTC
Cuts at: 3337 3337
Size: 5540
XbaI T'CTAG_A
Cuts at: 2571 2571
Size: 5540
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3035 3035
Size: 5540
XmaI C'CCGG_G
Cuts at: 2560 2560
Size: 5540
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI
PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI
BsmBI BspEI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
DraIII EcoNI EcoRV FseI FspAI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PpuMI
PshAI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI XmnI ZraI