(Circular) (Six-base) MAPSORT of: pF1KB8419.seq Check: 9531 from: 1 to: 4700 pF1KB8419 4700 bp With 182 enzymes: SgfI * June 20, 2008 10:28 .. AccI GT'mk_AC Cuts at: 491 1738 491 Size: 1247 3453 Acc65I G'GTAC_C Cuts at: 1716 1716 Size: 4700 AclI AA'CG_TT Cuts at: 4540 4540 Size: 4700 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 245 513 681 849 1437 1521 2525 2957 3310 Size: 268 168 168 588 84 1004 432 353 Cuts at: 3310 3972 245 Size: 662 973 Fragments arranged by size: 1004 973 662 588 432 353 268 168 168 84 AflIII A'CryG_T Cuts at: 2052 3852 4133 2052 Size: 1800 281 2619 Fragments arranged by size: 2619 1800 281 AleI CACnn'nnGTG Cuts at: 992 1387 992 Size: 395 4305 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 323 323 Size: 4700 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1626 1658 1626 Size: 32 4668 AlwNI CAG_nnn'CTG Cuts at: 2005 3443 2005 Size: 1438 3262 ApaLI G'TGCA_C Cuts at: 3538 3538 Size: 4700 ApoI r'AATT_y Cuts at: 396 477 1113 1571 1683 1704 4053 4309 396 Size: 81 636 458 112 21 2349 256 787 Fragments arranged by size: 2349 787 636 458 256 112 81 21 AseI AT'TA_AT Cuts at: 20 20 Size: 4700 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4700 AvaI C'yCGr_G Cuts at: 763 1720 763 Size: 957 3743 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 1500 1533 1500 Size: 33 4667 BamHI G'GATC_C Cuts at: 1725 1725 Size: 4700 BanI G'GyrC_C Cuts at: 1478 1716 2130 2378 2413 1478 Size: 238 414 248 35 3765 Fragments arranged by size: 3765 414 248 238 35 BanII G_rGCy'C Cuts at: 1714 2744 1714 Size: 1030 3670 BbeI G_GCGC'C Cuts at: 2134 2382 2134 Size: 248 4452 BciVI GTATCCnnnnn_n' Cuts at: 2598 3654 4700 2598 Size: 1056 1046 2598 Fragments arranged by size: 2598 1056 1046 BclI T'GATC_A Cuts at: 2220 2220 Size: 4700 BglII A'GATC_T Cuts at: 2215 4252 2215 Size: 2037 2663 BlpI GC'TnA_GC Cuts at: 1812 1812 Size: 4700 Bme1580I G_kGCm'C Cuts at: 931 2325 2418 3542 931 Size: 1394 93 1124 2089 Fragments arranged by size: 2089 1394 1124 93 BmrI ACTGGGnnnn_n' Cuts at: 1912 1993 2328 4472 1912 Size: 81 335 2144 2140 Fragments arranged by size: 2144 2140 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3067 3067 Size: 4700 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 929 929 Size: 4700 Bpu10I CC'TnA_GC Cuts at: 1291 1291 Size: 4700 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1036 1876 2755 3469 3767 1036 Size: 840 879 714 298 1969 Fragments arranged by size: 1969 879 840 714 298 BsaI GGTCTCn'nnnn_ Cuts at: 138 4404 138 Size: 4266 434 BsaAI yAC'GTr Cuts at: 1578 2053 1578 Size: 475 4225 BsaBI GATnn'nnATC Cuts at: 6 2214 4251 6 Size: 2208 2037 455 Fragments arranged by size: 2208 2037 455 BsaHI Gr'CG_yC Cuts at: 2131 2379 3081 4577 2131 Size: 248 702 1496 2254 Fragments arranged by size: 2254 1496 702 248 BsaWI w'CCGG_w Cuts at: 1481 1889 2410 3088 3499 3646 3920 1481 Size: 408 521 678 411 147 274 2261 Fragments arranged by size: 2261 678 521 411 408 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 427 427 Size: 4700 BseYI C'CCAG_C Cuts at: 2125 2883 3548 3956 2125 Size: 758 665 408 2869 Fragments arranged by size: 2869 758 665 408 BsiEI CG_ry'CG Cuts at: 104 2242 2288 3518 104 Size: 2138 46 1230 1286 Fragments arranged by size: 2138 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 1714 2492 2682 3542 1714 Size: 778 190 860 2872 Fragments arranged by size: 2872 860 778 190 BsmI GAATG_Cn' Cuts at: 653 1234 653 Size: 581 4119 BsmBI CGTCTCn'nnnn_ Cuts at: 1683 1683 Size: 4700 Bsp1286I G_dGCh'C Cuts at: 931 1714 2325 2418 2492 2682 2744 3542 931 Size: 783 611 93 74 190 62 798 2089 Fragments arranged by size: 2089 798 783 611 190 93 74 62 BspHI T'CATG_A Cuts at: 354 1620 3132 354 Size: 1266 1512 1922 Fragments arranged by size: 1922 1512 1266 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1750 1964 2266 2647 1750 Size: 214 302 381 3803 Fragments arranged by size: 3803 381 302 214 BsrBI CCG'CTC Cuts at: 50 2992 3046 4530 4695 50 Size: 2942 54 1484 165 55 Fragments arranged by size: 2942 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1221 2612 1221 Size: 1391 3309 BsrFI r'CCGG_y Cuts at: 2698 2879 2698 Size: 181 4519 BssSI C'ACGA_G Cuts at: 2971 3679 2971 Size: 708 3992 BstAPI GCAn_nnn'nTGC Cuts at: 2077 2077 Size: 4700 BstBI TT'CG_AA Cuts at: 3061 3061 Size: 4700 BstXI CCAn_nnnn'nTGG Cuts at: 329 329 Size: 4700 BstYI r'GATC_y Cuts at: 1 1725 2192 2215 2550 2796 3102 3114 3200 Size: 1724 467 23 335 246 306 12 86 Cuts at: 3200 4252 1 Size: 1052 449 Fragments arranged by size: 1724 1052 467 449 335 306 246 86 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 4700 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2650 2831 2994 2650 Size: 181 163 4356 Fragments arranged by size: 4356 181 163 BtsI GCAGTG_nn' Cuts at: 1943 1943 Size: 4700 DraI TTT'AAA Cuts at: 1699 1699 Size: 4700 DrdI GACnn_nn'nnGTC Cuts at: 2406 3750 2406 Size: 1344 3356 EaeI y'GGCC_r Cuts at: 2285 2459 2850 2877 4222 2285 Size: 174 391 27 1345 2763 Fragments arranged by size: 2763 1345 391 174 27 EagI C'GGCC_G Cuts at: 2285 2285 Size: 4700 EarI CTCTTCn'nnn_ Cuts at: 769 937 1273 1671 2723 2933 769 Size: 168 336 398 1052 210 2536 Fragments arranged by size: 2536 1052 398 336 210 168 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2908 3650 3796 4510 2908 Size: 742 146 714 3098 Fragments arranged by size: 3098 742 714 146 EcoICRI GAG'CTC Cuts at: 1712 1712 Size: 4700 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 245 513 681 849 929 1437 1521 2525 2957 Size: 268 168 168 80 508 84 1004 432 Cuts at: 2957 3310 3972 245 Size: 353 662 973 Fragments arranged by size: 1004 973 662 508 432 353 268 168 168 84 80 EcoNI CCTnn'n_nnAGG Cuts at: 182 182 Size: 4700 EcoO109I rG'GnC_Cy Cuts at: 563 1487 1839 563 Size: 924 352 3424 Fragments arranged by size: 3424 924 352 EcoRI G'AATT_C Cuts at: 477 1704 477 Size: 1227 3473 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2719 2719 Size: 4700 FspI TGC'GCA Cuts at: 2481 2481 Size: 4700 HaeII r_GCGC'y Cuts at: 2134 2382 3612 4383 2134 Size: 248 1230 771 2451 Fragments arranged by size: 2451 1230 771 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 336 364 368 396 624 656 936 968 2542 Size: 28 4 28 228 32 280 32 1574 Cuts at: 2542 2572 2574 2604 336 Size: 30 2 30 2432 Fragments arranged by size: 2432 1574 280 228 32 32 30 30 28 28 4 2 HincII GTy'rAC Cuts at: 1739 1739 Size: 4700 HindIII A'AGCT_T Cuts at: 525 693 1029 1449 525 Size: 168 336 420 3776 Fragments arranged by size: 3776 420 336 168 KasI G'GCGC_C Cuts at: 2130 2378 2130 Size: 248 4452 KpnI G_GTAC'C Cuts at: 1720 1720 Size: 4700 MluI A'CGCG_T Cuts at: 4133 4133 Size: 4700 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 499 3459 3643 499 Size: 2960 184 1556 Fragments arranged by size: 2960 1556 184 MscI TGG'CCA Cuts at: 2461 2461 Size: 4700 MslI CAynn'nnrTG Cuts at: 305 327 458 824 840 875 992 1260 1387 Size: 22 131 366 16 35 117 268 127 Cuts at: 1387 2689 2816 305 Size: 1302 127 2189 Fragments arranged by size: 2189 1302 366 268 131 127 127 117 35 22 16 MspA1I CmG'CkG Cuts at: 981 1523 1811 2125 2485 3269 3514 981 Size: 542 288 314 360 784 245 2167 Fragments arranged by size: 2167 784 542 360 314 288 245 NaeI GCC'GGC Cuts at: 2881 2881 Size: 4700 NarI GG'CG_CC Cuts at: 2131 2379 2131 Size: 248 4452 NcoI C'CATG_G Cuts at: 107 107 Size: 4700 NdeI CA'TA_TG Cuts at: 837 837 Size: 4700 NgoMIV G'CCGG_C Cuts at: 2879 2879 Size: 4700 NspI r_CATG'y Cuts at: 1753 2253 3856 1753 Size: 500 1603 2597 Fragments arranged by size: 2597 1603 500 PciI A'CATG_T Cuts at: 3852 3852 Size: 4700 PflMI CCAn_nnn'nTGG Cuts at: 1105 1105 Size: 4700 PmeI GTTT'AAAC Cuts at: 1699 1699 Size: 4700 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3146 3178 3146 Size: 32 4668 PpuMI rG'GwC_Cy Cuts at: 563 1487 563 Size: 924 3776 PsiI TTA'TAA Cuts at: 296 587 923 1427 296 Size: 291 336 504 3569 Fragments arranged by size: 3569 504 336 291 PstI C_TGCA'G Cuts at: 1747 2432 1747 Size: 685 4015 PvuI CG_AT'CG Cuts at: 104 104 Size: 4700 PvuII CAG'CTG Cuts at: 1523 2125 2485 1523 Size: 602 360 3738 Fragments arranged by size: 3738 602 360 RsrII CG'GwC_CG Cuts at: 2895 2895 Size: 4700 SacI G_AGCT'C Cuts at: 1714 1714 Size: 4700 SalI G'TCGA_C Cuts at: 1737 1737 Size: 4700 SapI GCTCTTCn'nnn_ Cuts at: 2723 2933 2723 Size: 210 4490 SbfI CC_TGCA'GG Cuts at: 1747 1747 Size: 4700 ScaI AGT'ACT Cuts at: 4217 4217 Size: 4700 SfcI C'TryA_G Cuts at: 33 1743 2428 3396 3587 33 Size: 1710 685 968 191 1146 Fragments arranged by size: 1710 1146 968 685 191 SfoI GGC'GCC Cuts at: 2132 2380 2132 Size: 248 4452 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4700 SmaI CCC'GGG Cuts at: 1722 1722 Size: 4700 SmlI C'TyrA_G Cuts at: 1015 1855 2770 3484 3746 1015 Size: 840 915 714 262 1969 Fragments arranged by size: 1969 915 840 714 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4700 SphI G_CATG'C Cuts at: 1753 2253 1753 Size: 500 4200 StyI C'CwwG_G Cuts at: 107 1834 2188 107 Size: 1727 354 2619 Fragments arranged by size: 2619 1727 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2283 2416 2875 3084 2283 Size: 133 459 209 3899 Fragments arranged by size: 3899 459 209 133 TatI w'GTAC_w Cuts at: 4215 4215 Size: 4700 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4217 4249 4217 Size: 32 4668 Tth111I GACn'n_nGTC Cuts at: 2497 2497 Size: 4700 XbaI T'CTAG_A Cuts at: 1731 1731 Size: 4700 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2195 2195 Size: 4700 XmaI C'CCGG_G Cuts at: 1720 1720 Size: 4700 XmnI GAAnn'nnTTC Cuts at: 215 526 694 778 1366 1534 215 Size: 311 168 84 588 168 3381 Fragments arranged by size: 3381 588 311 168 168 84 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AgeI AhdI ApaI AscI AvrII BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI BsaXI BsgI BsiWI BspEI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI NheI NotI NruI NsiI PacI PasI PfoI PmlI PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI