Restriction map for
pF1KB8426
(Circular) (Six-base) MAPSORT of: pF1KB8426.seq Check: 5892 from: 1 to: 3767
pF1KB8426 3767 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 805 805
Size: 3767
Acc65I G'GTAC_C
Cuts at: 783 783
Size: 3767
AclI AA'CG_TT
Cuts at: 3607 3607
Size: 3767
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1592 2024 2377 3039 1592
Size: 432 353 662 2320
Fragments arranged by size:
2320 662 432 353
AflIII A'CryG_T
Cuts at: 1119 2919 3200 1119
Size: 1800 281 1686
Fragments arranged by size:
1800 1686 281
AlwNI CAG_nnn'CTG
Cuts at: 178 1072 2510 178
Size: 894 1438 1435
Fragments arranged by size:
1438 1435 894
ApaLI G'TGCA_C
Cuts at: 2605 2605
Size: 3767
ApoI r'AATT_y
Cuts at: 217 253 771 3120 3376 217
Size: 36 518 2349 256 608
Fragments arranged by size:
2349 608 518 256 36
AseI AT'TA_AT
Cuts at: 20 20
Size: 3767
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3767
AvaI C'yCGr_G
Cuts at: 787 787
Size: 3767
BamHI G'GATC_C
Cuts at: 792 792
Size: 3767
BanI G'GyrC_C
Cuts at: 783 1197 1445 1480 783
Size: 414 248 35 3070
Fragments arranged by size:
3070 414 248 35
BanII G_rGCy'C
Cuts at: 375 676 781 1811 375
Size: 301 105 1030 2331
Fragments arranged by size:
2331 1030 301 105
BbeI G_GCGC'C
Cuts at: 1201 1449 1201
Size: 248 3519
BbvCI CC'TCA_GC
Cuts at: 712 712
Size: 3767
BciVI GTATCCnnnnn_n'
Cuts at: 303 1665 2721 3767 303
Size: 1362 1056 1046 303
Fragments arranged by size:
1362 1056 1046 303
BclI T'GATC_A
Cuts at: 467 1287 467
Size: 820 2947
BglII A'GATC_T
Cuts at: 1282 3319 1282
Size: 2037 1730
BlpI GC'TnA_GC
Cuts at: 879 879
Size: 3767
Bme1580I G_kGCm'C
Cuts at: 1392 1485 2609 1392
Size: 93 1124 2550
Fragments arranged by size:
2550 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 979 1060 1395 3539 979
Size: 81 335 2144 1207
Fragments arranged by size:
2144 1207 335 81
BmtI G_CTAG'C
Cuts at: 451 451
Size: 3767
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2134 2134
Size: 3767
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 516 516
Size: 3767
Bpu10I CC'TnA_GC
Cuts at: 675 712 746 675
Size: 37 34 3696
Fragments arranged by size:
3696 37 34
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 527 723 943 1822 2536 2834 527
Size: 196 220 879 714 298 1460
Fragments arranged by size:
1460 879 714 298 220 196
BsaI GGTCTCn'nnnn_
Cuts at: 3471 3471
Size: 3767
BsaAI yAC'GTr
Cuts at: 1120 1120
Size: 3767
BsaBI GATnn'nnATC
Cuts at: 6 503 1281 3318 6
Size: 497 778 2037 455
Fragments arranged by size:
2037 778 497 455
BsaHI Gr'CG_yC
Cuts at: 1198 1446 2148 3644 1198
Size: 248 702 1496 1321
Fragments arranged by size:
1496 1321 702 248
BsaWI w'CCGG_w
Cuts at: 956 1477 2155 2566 2713 2987 956
Size: 521 678 411 147 274 1736
Fragments arranged by size:
1736 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 367 367
Size: 3767
BseYI C'CCAG_C
Cuts at: 1192 1950 2615 3023 1192
Size: 758 665 408 1936
Fragments arranged by size:
1936 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 368 1309 1355 2585 104
Size: 264 941 46 1230 1286
Fragments arranged by size:
1286 1230 941 264 46
BsiHKAI G_wGCw'C
Cuts at: 781 1559 1749 2609 781
Size: 778 190 860 1939
Fragments arranged by size:
1939 860 778 190
BsmI GAATG_Cn'
Cuts at: 449 449
Size: 3767
Bsp1286I G_dGCh'C
Cuts at: 375 676 781 1392 1485 1559 1749 1811 2609
Size: 301 105 611 93 74 190 62 798
Cuts at: 2609 375
Size: 1533
Fragments arranged by size:
1533 798 611 301 190 105 93 74
62
BspHI T'CATG_A
Cuts at: 2199 2199
Size: 3767
BspMI ACCTGCnnnn'nnnn_
Cuts at: 817 1031 1333 1714 817
Size: 214 302 381 2870
Fragments arranged by size:
2870 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2059 2113 3597 3762 50
Size: 2009 54 1484 165 55
Fragments arranged by size:
2009 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1679 1679
Size: 3767
BsrFI r'CCGG_y
Cuts at: 1765 1946 1765
Size: 181 3586
BsrGI T'GTAC_A
Cuts at: 609 609
Size: 3767
BssHII G'CGCG_C
Cuts at: 726 726
Size: 3767
BssSI C'ACGA_G
Cuts at: 2038 2746 2038
Size: 708 3059
BstAPI GCAn_nnn'nTGC
Cuts at: 188 1144 188
Size: 956 2811
BstBI TT'CG_AA
Cuts at: 2128 2128
Size: 3767
BstEII G'GTnAC_C
Cuts at: 716 716
Size: 3767
BstYI r'GATC_y
Cuts at: 1 792 1259 1282 1617 1863 2169 2181 2267
Size: 791 467 23 335 246 306 12 86
Cuts at: 2267 3319 1
Size: 1052 449
Fragments arranged by size:
1052 791 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 721 721
Size: 3767
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1717 1898 2061 1717
Size: 181 163 3423
Fragments arranged by size:
3423 181 163
BtsI GCAGTG_nn'
Cuts at: 1010 1010
Size: 3767
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 230 265 230
Size: 35 3732
DraI TTT'AAA
Cuts at: 766 766
Size: 3767
DrdI GACnn_nn'nnGTC
Cuts at: 123 1473 2817 123
Size: 1350 1344 1073
Fragments arranged by size:
1350 1344 1073
EaeI y'GGCC_r
Cuts at: 119 365 1352 1526 1917 1944 3289 119
Size: 246 987 174 391 27 1345 597
Fragments arranged by size:
1345 987 597 391 246 174 27
EagI C'GGCC_G
Cuts at: 365 1352 365
Size: 987 2780
EarI CTCTTCn'nnn_
Cuts at: 593 1790 2000 593
Size: 1197 210 2360
Fragments arranged by size:
2360 1197 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1975 2717 2863 3577 1975
Size: 742 146 714 2165
Fragments arranged by size:
2165 742 714 146
EcoICRI GAG'CTC
Cuts at: 779 779
Size: 3767
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 516 1592 2024 2377 3039 516
Size: 1076 432 353 662 1244
Fragments arranged by size:
1244 1076 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 492 735 906 492
Size: 243 171 3353
Fragments arranged by size:
3353 243 171
EcoRI G'AATT_C
Cuts at: 771 771
Size: 3767
EcoRV GAT'ATC
Cuts at: 390 390
Size: 3767
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1786 1786
Size: 3767
FspI TGC'GCA
Cuts at: 1548 1548
Size: 3767
HaeII r_GCGC'y
Cuts at: 1201 1449 2679 3450 1201
Size: 248 1230 771 1518
Fragments arranged by size:
1518 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1609 1639 1641 1671 1609
Size: 30 2 30 3705
Fragments arranged by size:
3705 30 30 2
HincII GTy'rAC
Cuts at: 431 806 431
Size: 375 3392
HpaI GTT'AAC
Cuts at: 431 431
Size: 3767
KasI G'GCGC_C
Cuts at: 1197 1445 1197
Size: 248 3519
KpnI G_GTAC'C
Cuts at: 787 787
Size: 3767
MluI A'CGCG_T
Cuts at: 3200 3200
Size: 3767
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2526 2710 2526
Size: 184 3583
MscI TGG'CCA
Cuts at: 1528 1528
Size: 3767
MslI CAynn'nnrTG
Cuts at: 1756 1883 1756
Size: 127 3640
MspA1I CmG'CkG
Cuts at: 711 723 878 1192 1552 2336 2581 711
Size: 12 155 314 360 784 245 1897
Fragments arranged by size:
1897 784 360 314 245 155 12
NaeI GCC'GGC
Cuts at: 1948 1948
Size: 3767
NarI GG'CG_CC
Cuts at: 1198 1446 1198
Size: 248 3519
NgoMIV G'CCGG_C
Cuts at: 1946 1946
Size: 3767
NheI G'CTAG_C
Cuts at: 447 447
Size: 3767
NspI r_CATG'y
Cuts at: 820 1320 2923 820
Size: 500 1603 1664
Fragments arranged by size:
1664 1603 500
PciI A'CATG_T
Cuts at: 2919 2919
Size: 3767
PmeI GTTT'AAAC
Cuts at: 766 766
Size: 3767
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2213 2245 2213
Size: 32 3735
PpuMI rG'GwC_Cy
Cuts at: 492 492
Size: 3767
PstI C_TGCA'G
Cuts at: 814 1499 814
Size: 685 3082
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3767
PvuII CAG'CTG
Cuts at: 711 1192 1552 711
Size: 481 360 2926
Fragments arranged by size:
2926 481 360
RsrII CG'GwC_CG
Cuts at: 1962 1962
Size: 3767
SacI G_AGCT'C
Cuts at: 781 781
Size: 3767
SacII CC_GC'GG
Cuts at: 724 724
Size: 3767
SalI G'TCGA_C
Cuts at: 804 804
Size: 3767
SapI GCTCTTCn'nnn_
Cuts at: 1790 2000 1790
Size: 210 3557
SbfI CC_TGCA'GG
Cuts at: 814 814
Size: 3767
ScaI AGT'ACT
Cuts at: 3284 3284
Size: 3767
SfcI C'TryA_G
Cuts at: 33 810 1495 2463 2654 33
Size: 777 685 968 191 1146
Fragments arranged by size:
1146 968 777 685 191
SfoI GGC'GCC
Cuts at: 1199 1447 1199
Size: 248 3519
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3767
SmaI CCC'GGG
Cuts at: 789 789
Size: 3767
SmlI C'TyrA_G
Cuts at: 542 702 922 1837 2551 2813 542
Size: 160 220 915 714 262 1496
Fragments arranged by size:
1496 915 714 262 220 160
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3767
SphI G_CATG'C
Cuts at: 820 1320 820
Size: 500 3267
StyI C'CwwG_G
Cuts at: 901 1255 901
Size: 354 3413
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1350 1483 1942 2151 1350
Size: 133 459 209 2966
Fragments arranged by size:
2966 459 209 133
TatI w'GTAC_w
Cuts at: 609 3282 609
Size: 2673 1094
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3284 3316 3284
Size: 32 3735
Tth111I GACn'n_nGTC
Cuts at: 1564 1564
Size: 3767
XbaI T'CTAG_A
Cuts at: 798 798
Size: 3767
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1262 1262
Size: 3767
XmaI C'CCGG_G
Cuts at: 787 787
Size: 3767
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI
BciVI BclI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NheI NspI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbsI BcgI BfrBI
BglI BmgBI BsaXI BsgI BsiWI BsmBI BspEI BstXI
BstZ17I Bsu36I ClaI DraIII EcoNI FseI FspAI HindIII
MfeI NcoI NdeI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI