Restriction map for
pF1KB8428
(Circular) (Six-base) MAPSORT of: pF1KB8428.seq Check: 4508 from: 1 to: 4514
pF1KB8428 4514 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 816 1051 816
Size: 235 4279
AccI GT'mk_AC
Cuts at: 1552 1552
Size: 4514
Acc65I G'GTAC_C
Cuts at: 1530 1530
Size: 4514
AclI AA'CG_TT
Cuts at: 4354 4354
Size: 4514
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 700 2339 2771 3124 3786 700
Size: 1639 432 353 662 1428
Fragments arranged by size:
1639 1428 662 432 353
AflII C'TTAA_G
Cuts at: 745 745
Size: 4514
AflIII A'CryG_T
Cuts at: 1193 1866 3666 3947 1193
Size: 673 1800 281 1760
Fragments arranged by size:
1800 1760 673 281
AgeI A'CCGG_T
Cuts at: 635 635
Size: 4514
AhdI GACnn_n'nnGTC
Cuts at: 1053 1053
Size: 4514
AleI CACnn'nnGTG
Cuts at: 701 701
Size: 4514
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1265 1265
Size: 4514
AlwNI CAG_nnn'CTG
Cuts at: 172 577 858 909 918 1140 1278 1378 1819
Size: 405 281 51 9 222 138 100 441
Cuts at: 1819 3257 172
Size: 1438 1429
Fragments arranged by size:
1438 1429 441 405 281 222 138 100
51 9
ApaI G_GGCC'C
Cuts at: 1327 1327
Size: 4514
ApaLI G'TGCA_C
Cuts at: 1189 3352 1189
Size: 2163 2351
ApoI r'AATT_y
Cuts at: 1518 3867 4123 1518
Size: 2349 256 1909
Fragments arranged by size:
2349 1909 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4514
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4514
AvaI C'yCGr_G
Cuts at: 145 256 1304 1534 145
Size: 111 1048 230 3125
Fragments arranged by size:
3125 1048 230 111
BamHI G'GATC_C
Cuts at: 1539 1539
Size: 4514
BanI G'GyrC_C
Cuts at: 1283 1530 1944 2192 2227 1283
Size: 247 414 248 35 3570
Fragments arranged by size:
3570 414 248 247 35
BanII G_rGCy'C
Cuts at: 373 1168 1238 1327 1528 2558 373
Size: 795 70 89 201 1030 2329
Fragments arranged by size:
2329 1030 795 201 89 70
BbeI G_GCGC'C
Cuts at: 1948 2196 1948
Size: 248 4266
BbsI GAAGACnn'nnnn_
Cuts at: 680 680
Size: 4514
BbvCI CC'TCA_GC
Cuts at: 595 595
Size: 4514
BciVI GTATCCnnnnn_n'
Cuts at: 337 2412 3468 4514 337
Size: 2075 1056 1046 337
Fragments arranged by size:
2075 1056 1046 337
BclI T'GATC_A
Cuts at: 920 2034 920
Size: 1114 3400
BglII A'GATC_T
Cuts at: 2029 4066 2029
Size: 2037 2477
BlpI GC'TnA_GC
Cuts at: 707 1162 1364 1626 707
Size: 455 202 262 3595
Fragments arranged by size:
3595 455 262 202
Bme1580I G_kGCm'C
Cuts at: 773 1193 1327 2139 2232 3356 773
Size: 420 134 812 93 1124 1931
Fragments arranged by size:
1931 1124 812 420 134 93
BmgBI CAC'GTC
Cuts at: 454 454
Size: 4514
BmrI ACTGGGnnnn_n'
Cuts at: 320 1726 1807 2142 4286 320
Size: 1406 81 335 2144 548
Fragments arranged by size:
2144 1406 548 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1488 2881 1488
Size: 1393 3121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1165 1458 1165
Size: 293 4221
Bpu10I CC'TnA_GC
Cuts at: 595 595
Size: 4514
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 817 1222 1690 2569 3283 3581 817
Size: 405 468 879 714 298 1750
Fragments arranged by size:
1750 879 714 468 405 298
BsaI GGTCTCn'nnnn_
Cuts at: 4218 4218
Size: 4514
BsaAI yAC'GTr
Cuts at: 1867 1867
Size: 4514
BsaBI GATnn'nnATC
Cuts at: 6 2028 4065 6
Size: 2022 2037 455
Fragments arranged by size:
2037 2022 455
BsaHI Gr'CG_yC
Cuts at: 1945 2193 2895 4391 1945
Size: 248 702 1496 2068
Fragments arranged by size:
2068 1496 702 248
BsaWI w'CCGG_w
Cuts at: 635 812 1703 2224 2902 3313 3460 3734 635
Size: 177 891 521 678 411 147 274 1415
Fragments arranged by size:
1415 891 678 521 411 274 177 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 502 517 532 547 502
Size: 15 15 15 4469
Fragments arranged by size:
4469 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 478 850 853 1195 478
Size: 372 3 342 3797
Fragments arranged by size:
3797 372 342 3
BseYI C'CCAG_C
Cuts at: 766 1939 2697 3362 3770 766
Size: 1173 758 665 408 1510
Fragments arranged by size:
1510 1173 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 476 526 476
Size: 50 4464
BsiEI CG_ry'CG
Cuts at: 104 2056 2102 3332 104
Size: 1952 46 1230 1286
Fragments arranged by size:
1952 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1193 1238 1528 2306 2496 3356 1193
Size: 45 290 778 190 860 2351
Fragments arranged by size:
2351 860 778 290 190 45
BsmBI CGTCTCn'nnnn_
Cuts at: 808 808
Size: 4514
Bsp1286I G_dGCh'C
Cuts at: 373 773 1168 1193 1238 1327 1528 2139 2232
Size: 400 395 25 45 89 201 611 93
Cuts at: 2232 2306 2496 2558 3356 373
Size: 74 190 62 798 1531
Fragments arranged by size:
1531 798 611 400 395 201 190 93
89 74 62 45 25
BspHI T'CATG_A
Cuts at: 1290 2946 1290
Size: 1656 2858
BspMI ACCTGCnnnn'nnnn_
Cuts at: 620 816 854 917 1051 1564 1778 2080 2461
Size: 196 38 63 134 513 214 302 381
Cuts at: 2461 620
Size: 2673
Fragments arranged by size:
2673 513 381 302 214 196 134 63
38
BsrBI CCG'CTC
Cuts at: 50 687 888 2806 2860 4344 4509 50
Size: 637 201 1918 54 1484 165 55
Fragments arranged by size:
1918 1484 637 201 165 55 54
BsrDI GCAATG_nn'
Cuts at: 440 2426 440
Size: 1986 2528
BsrFI r'CCGG_y
Cuts at: 635 1011 1368 1506 2512 2693 635
Size: 376 357 138 1006 181 2456
Fragments arranged by size:
2456 1006 376 357 181 138
BssSI C'ACGA_G
Cuts at: 2785 3493 2785
Size: 708 3806
BstAPI GCAn_nnn'nTGC
Cuts at: 1278 1891 1278
Size: 613 3901
BstBI TT'CG_AA
Cuts at: 2875 2875
Size: 4514
BstYI r'GATC_y
Cuts at: 1 1539 2006 2029 2364 2610 2916 2928 3014
Size: 1538 467 23 335 246 306 12 86
Cuts at: 3014 4066 1
Size: 1052 449
Fragments arranged by size:
1538 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4514
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2464 2645 2808 2464
Size: 181 163 4170
Fragments arranged by size:
4170 181 163
BtsI GCAGTG_nn'
Cuts at: 316 938 1379 1757 316
Size: 622 441 378 3073
Fragments arranged by size:
3073 622 441 378
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1085 1120 1085
Size: 35 4479
DraI TTT'AAA
Cuts at: 1513 1513
Size: 4514
DrdI GACnn_nn'nnGTC
Cuts at: 2220 3564 2220
Size: 1344 3170
EaeI y'GGCC_r
Cuts at: 336 2099 2273 2664 2691 4036 336
Size: 1763 174 391 27 1345 814
Fragments arranged by size:
1763 1345 814 391 174 27
EagI C'GGCC_G
Cuts at: 2099 2099
Size: 4514
EarI CTCTTCn'nnn_
Cuts at: 2537 2747 2537
Size: 210 4304
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 798 2722 3464 3610 4324 798
Size: 1924 742 146 714 988
Fragments arranged by size:
1924 988 742 714 146
EcoICRI GAG'CTC
Cuts at: 1236 1526 1236
Size: 290 4224
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 700 1165 1458 2339 2771 3124 3786 700
Size: 465 293 881 432 353 662 1428
Fragments arranged by size:
1428 881 662 465 432 353 293
EcoNI CCTnn'n_nnAGG
Cuts at: 486 850 486
Size: 364 4150
EcoO109I rG'GnC_Cy
Cuts at: 906 969 1125 1323 1324 1653 906
Size: 63 156 198 1 329 3767
Fragments arranged by size:
3767 329 198 156 63 1
EcoRI G'AATT_C
Cuts at: 1518 1518
Size: 4514
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2533 2533
Size: 4514
FspI TGC'GCA
Cuts at: 862 2295 862
Size: 1433 3081
HaeII r_GCGC'y
Cuts at: 1948 2196 3426 4197 1948
Size: 248 1230 771 2265
Fragments arranged by size:
2265 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 154 186 1039 1071 2356 2386 2388 2418 154
Size: 32 853 32 1285 30 2 30 2250
Fragments arranged by size:
2250 1285 853 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1553 1553
Size: 4514
HindIII A'AGCT_T
Cuts at: 709 709
Size: 4514
KasI G'GCGC_C
Cuts at: 1944 2192 1944
Size: 248 4266
KpnI G_GTAC'C
Cuts at: 1534 1534
Size: 4514
MluI A'CGCG_T
Cuts at: 3947 3947
Size: 4514
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3273 3457 3273
Size: 184 4330
MscI TGG'CCA
Cuts at: 338 2275 338
Size: 1937 2577
MslI CAynn'nnrTG
Cuts at: 554 701 1289 1420 2503 2630 554
Size: 147 588 131 1083 127 2438
Fragments arranged by size:
2438 1083 588 147 131 127
MspA1I CmG'CkG
Cuts at: 460 928 951 1625 1939 2299 3083 3328 460
Size: 468 23 674 314 360 784 245 1646
Fragments arranged by size:
1646 784 674 468 360 314 245 23
NaeI GCC'GGC
Cuts at: 1013 1370 2695 1013
Size: 357 1325 2832
Fragments arranged by size:
2832 1325 357
NarI GG'CG_CC
Cuts at: 1945 2193 1945
Size: 248 4266
NcoI C'CATG_G
Cuts at: 107 107
Size: 4514
NgoMIV G'CCGG_C
Cuts at: 1011 1368 2693 1011
Size: 357 1325 2832
Fragments arranged by size:
2832 1325 357
NspI r_CATG'y
Cuts at: 267 1197 1567 2067 3670 267
Size: 930 370 500 1603 1111
Fragments arranged by size:
1603 1111 930 500 370
PciI A'CATG_T
Cuts at: 1193 3666 1193
Size: 2473 2041
PmeI GTTT'AAAC
Cuts at: 1513 1513
Size: 4514
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2960 2992 2960
Size: 32 4482
PpuMI rG'GwC_Cy
Cuts at: 906 906
Size: 4514
PshAI GACnn'nnGTC
Cuts at: 1464 1464
Size: 4514
PspOMI G'GGCC_C
Cuts at: 1323 1323
Size: 4514
PstI C_TGCA'G
Cuts at: 914 945 1032 1062 1274 1561 2246 914
Size: 31 87 30 212 287 685 3182
Fragments arranged by size:
3182 685 287 212 87 31 30
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4514
PvuII CAG'CTG
Cuts at: 1939 2299 1939
Size: 360 4154
RsrII CG'GwC_CG
Cuts at: 2709 2709
Size: 4514
SacI G_AGCT'C
Cuts at: 1238 1528 1238
Size: 290 4224
SalI G'TCGA_C
Cuts at: 1551 1551
Size: 4514
SapI GCTCTTCn'nnn_
Cuts at: 2537 2747 2537
Size: 210 4304
SbfI CC_TGCA'GG
Cuts at: 1561 1561
Size: 4514
ScaI AGT'ACT
Cuts at: 4031 4031
Size: 4514
SexAI A'CCwGG_T
Cuts at: 1217 1217
Size: 4514
SfcI C'TryA_G
Cuts at: 33 850 910 941 1028 1058 1270 1557 2242
Size: 817 60 31 87 30 212 287 685
Cuts at: 2242 3210 3401 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 817 685 287 212 191 87
60 31 30
SfoI GGC'GCC
Cuts at: 1946 2194 1946
Size: 248 4266
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4514
SmaI CCC'GGG
Cuts at: 1306 1536 1306
Size: 230 4284
SmlI C'TyrA_G
Cuts at: 745 796 1237 1669 2584 3298 3560 745
Size: 51 441 432 915 714 262 1699
Fragments arranged by size:
1699 915 714 441 432 262 51
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4514
SphI G_CATG'C
Cuts at: 267 1567 2067 267
Size: 1300 500 2714
Fragments arranged by size:
2714 1300 500
SrfI GCCC'GGGC
Cuts at: 1306 1306
Size: 4514
StuI AGG'CCT
Cuts at: 1246 1246
Size: 4514
StyI C'CwwG_G
Cuts at: 107 388 1403 1648 2002 107
Size: 281 1015 245 354 2619
Fragments arranged by size:
2619 1015 354 281 245
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2097 2230 2689 2898 2097
Size: 133 459 209 3713
Fragments arranged by size:
3713 459 209 133
TatI w'GTAC_w
Cuts at: 518 4029 518
Size: 3511 1003
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4031 4063 4031
Size: 32 4482
Tth111I GACn'n_nGTC
Cuts at: 1391 2311 1391
Size: 920 3594
XbaI T'CTAG_A
Cuts at: 138 1545 138
Size: 1407 3107
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1419 2009 1419
Size: 590 3924
XmaI C'CCGG_G
Cuts at: 1304 1534 1304
Size: 230 4284
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AloI AscI AvrII BaeI BcgI BfrBI
BglI BmtI BsiWI BsmI BspEI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI DraIII EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PsiI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SspI SwaI XhoI XmnI
ZraI