Restriction map for
pF1KB8430
(Circular) (Six-base) MAPSORT of: pF1KB8430.seq Check: 463 from: 1 to: 3884
pF1KB8430 3884 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 922 922
Size: 3884
Acc65I G'GTAC_C
Cuts at: 900 900
Size: 3884
AclI AA'CG_TT
Cuts at: 3724 3724
Size: 3884
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 1709 2141 2494 3156 376
Size: 1333 432 353 662 1104
Fragments arranged by size:
1333 1104 662 432 353
AflIII A'CryG_T
Cuts at: 1236 3036 3317 1236
Size: 1800 281 1803
Fragments arranged by size:
1803 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 1189 2627 1189
Size: 1438 2446
ApaLI G'TGCA_C
Cuts at: 2722 2722
Size: 3884
ApoI r'AATT_y
Cuts at: 405 888 3237 3493 405
Size: 483 2349 256 796
Fragments arranged by size:
2349 796 483 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3884
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3884
AvaI C'yCGr_G
Cuts at: 904 904
Size: 3884
BamHI G'GATC_C
Cuts at: 909 909
Size: 3884
BanI G'GyrC_C
Cuts at: 900 1314 1562 1597 900
Size: 414 248 35 3187
Fragments arranged by size:
3187 414 248 35
BanII G_rGCy'C
Cuts at: 533 898 1928 533
Size: 365 1030 2489
Fragments arranged by size:
2489 1030 365
BbeI G_GCGC'C
Cuts at: 1318 1566 1318
Size: 248 3636
BbsI GAAGACnn'nnnn_
Cuts at: 188 188
Size: 3884
BciVI GTATCCnnnnn_n'
Cuts at: 1782 2838 3884 1782
Size: 1056 1046 1782
Fragments arranged by size:
1782 1056 1046
BclI T'GATC_A
Cuts at: 1404 1404
Size: 3884
BglII A'GATC_T
Cuts at: 326 606 1399 3436 326
Size: 280 793 2037 774
Fragments arranged by size:
2037 793 774 280
BlpI GC'TnA_GC
Cuts at: 996 996
Size: 3884
Bme1580I G_kGCm'C
Cuts at: 1509 1602 2726 1509
Size: 93 1124 2667
Fragments arranged by size:
2667 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1096 1177 1512 3656 1096
Size: 81 335 2144 1324
Fragments arranged by size:
2144 1324 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2251 2251
Size: 3884
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 698 713 448
Size: 250 15 3619
Fragments arranged by size:
3619 250 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1060 1939 2653 2951 1060
Size: 879 714 298 1993
Fragments arranged by size:
1993 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3588 3588
Size: 3884
BsaAI yAC'GTr
Cuts at: 1237 1237
Size: 3884
BsaBI GATnn'nnATC
Cuts at: 6 1398 3435 6
Size: 1392 2037 455
Fragments arranged by size:
2037 1392 455
BsaHI Gr'CG_yC
Cuts at: 1315 1563 2265 3761 1315
Size: 248 702 1496 1438
Fragments arranged by size:
1496 1438 702 248
BsaWI w'CCGG_w
Cuts at: 1073 1594 2272 2683 2830 3104 1073
Size: 521 678 411 147 274 1853
Fragments arranged by size:
1853 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1309 2067 2732 3140 1309
Size: 758 665 408 2053
Fragments arranged by size:
2053 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 125 1426 1472 2702 104
Size: 21 1301 46 1230 1286
Fragments arranged by size:
1301 1286 1230 46 21
BsiHKAI G_wGCw'C
Cuts at: 898 1676 1866 2726 898
Size: 778 190 860 2056
Fragments arranged by size:
2056 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 533 898 1509 1602 1676 1866 1928 2726 533
Size: 365 611 93 74 190 62 798 1691
Fragments arranged by size:
1691 798 611 365 190 93 74 62
BspHI T'CATG_A
Cuts at: 2316 2316
Size: 3884
BspMI ACCTGCnnnn'nnnn_
Cuts at: 934 1148 1450 1831 934
Size: 214 302 381 2987
Fragments arranged by size:
2987 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2176 2230 3714 3879 50
Size: 2126 54 1484 165 55
Fragments arranged by size:
2126 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1796 1796
Size: 3884
BsrFI r'CCGG_y
Cuts at: 1882 2063 1882
Size: 181 3703
BssSI C'ACGA_G
Cuts at: 2155 2863 2155
Size: 708 3176
BstAPI GCAn_nnn'nTGC
Cuts at: 1261 1261
Size: 3884
BstBI TT'CG_AA
Cuts at: 2245 2245
Size: 3884
BstYI r'GATC_y
Cuts at: 1 326 606 909 1376 1399 1734 1980 2286
Size: 325 280 303 467 23 335 246 306
Cuts at: 2286 2298 2384 3436 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 467 449 335 325 306 303 280
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3884
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1834 2015 2178 1834
Size: 181 163 3540
Fragments arranged by size:
3540 181 163
BtsI GCAGTG_nn'
Cuts at: 407 770 1127 407
Size: 363 357 3164
Fragments arranged by size:
3164 363 357
DraI TTT'AAA
Cuts at: 883 883
Size: 3884
DrdI GACnn_nn'nnGTC
Cuts at: 1590 2934 1590
Size: 1344 2540
EaeI y'GGCC_r
Cuts at: 1469 1643 2034 2061 3406 1469
Size: 174 391 27 1345 1947
Fragments arranged by size:
1947 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1469 1469
Size: 3884
EarI CTCTTCn'nnn_
Cuts at: 270 1907 2117 270
Size: 1637 210 2037
Fragments arranged by size:
2037 1637 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2092 2834 2980 3694 2092
Size: 742 146 714 2282
Fragments arranged by size:
2282 742 714 146
EcoICRI GAG'CTC
Cuts at: 896 896
Size: 3884
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 448 698 713 1709 2141 2494 3156 376
Size: 72 250 15 996 432 353 662 1104
Fragments arranged by size:
1104 996 662 432 353 250 72 15
EcoO109I rG'GnC_Cy
Cuts at: 1023 1023
Size: 3884
EcoRI G'AATT_C
Cuts at: 888 888
Size: 3884
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1903 1903
Size: 3884
FspI TGC'GCA
Cuts at: 1665 1665
Size: 3884
HaeII r_GCGC'y
Cuts at: 1318 1566 2796 3567 1318
Size: 248 1230 771 1635
Fragments arranged by size:
1635 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 776 808 1726 1756 1758 1788 776
Size: 32 918 30 2 30 2872
Fragments arranged by size:
2872 918 32 30 30 2
HincII GTy'rAC
Cuts at: 923 923
Size: 3884
KasI G'GCGC_C
Cuts at: 1314 1562 1314
Size: 248 3636
KpnI G_GTAC'C
Cuts at: 904 904
Size: 3884
MluI A'CGCG_T
Cuts at: 3317 3317
Size: 3884
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 232 2643 2827 232
Size: 2411 184 1289
Fragments arranged by size:
2411 1289 184
MscI TGG'CCA
Cuts at: 1645 1645
Size: 3884
MslI CAynn'nnrTG
Cuts at: 466 1873 2000 466
Size: 1407 127 2350
Fragments arranged by size:
2350 1407 127
MspA1I CmG'CkG
Cuts at: 808 995 1309 1669 2453 2698 808
Size: 187 314 360 784 245 1994
Fragments arranged by size:
1994 784 360 314 245 187
NaeI GCC'GGC
Cuts at: 2065 2065
Size: 3884
NarI GG'CG_CC
Cuts at: 1315 1563 1315
Size: 248 3636
NcoI C'CATG_G
Cuts at: 107 107
Size: 3884
NgoMIV G'CCGG_C
Cuts at: 2063 2063
Size: 3884
NspI r_CATG'y
Cuts at: 937 1437 3040 937
Size: 500 1603 1781
Fragments arranged by size:
1781 1603 500
PasI CC'CwG_GG
Cuts at: 481 481
Size: 3884
PciI A'CATG_T
Cuts at: 3036 3036
Size: 3884
PmeI GTTT'AAAC
Cuts at: 883 883
Size: 3884
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2330 2362 2330
Size: 32 3852
PstI C_TGCA'G
Cuts at: 931 1616 931
Size: 685 3199
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3884
PvuII CAG'CTG
Cuts at: 808 1309 1669 808
Size: 501 360 3023
Fragments arranged by size:
3023 501 360
RsrII CG'GwC_CG
Cuts at: 2079 2079
Size: 3884
SacI G_AGCT'C
Cuts at: 898 898
Size: 3884
SalI G'TCGA_C
Cuts at: 921 921
Size: 3884
SapI GCTCTTCn'nnn_
Cuts at: 1907 2117 1907
Size: 210 3674
SbfI CC_TGCA'GG
Cuts at: 931 931
Size: 3884
ScaI AGT'ACT
Cuts at: 3401 3401
Size: 3884
SfcI C'TryA_G
Cuts at: 33 600 927 1612 2580 2771 33
Size: 567 327 685 968 191 1146
Fragments arranged by size:
1146 968 685 567 327 191
SfoI GGC'GCC
Cuts at: 1316 1564 1316
Size: 248 3636
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3884
SmaI CCC'GGG
Cuts at: 906 906
Size: 3884
SmlI C'TyrA_G
Cuts at: 1039 1954 2668 2930 1039
Size: 915 714 262 1993
Fragments arranged by size:
1993 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3884
SphI G_CATG'C
Cuts at: 937 1437 937
Size: 500 3384
SspI AAT'ATT
Cuts at: 392 392
Size: 3884
StyI C'CwwG_G
Cuts at: 107 255 1018 1372 107
Size: 148 763 354 2619
Fragments arranged by size:
2619 763 354 148
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1467 1600 2059 2268 1467
Size: 133 459 209 3083
Fragments arranged by size:
3083 459 209 133
TatI w'GTAC_w
Cuts at: 3399 3399
Size: 3884
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 690 722 3401 3433 690
Size: 32 2679 32 1141
Fragments arranged by size:
2679 1141 32 32
Tth111I GACn'n_nGTC
Cuts at: 1681 1681
Size: 3884
XbaI T'CTAG_A
Cuts at: 915 915
Size: 3884
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1379 1379
Size: 3884
XmaI C'CCGG_G
Cuts at: 904 904
Size: 3884
XmnI GAAnn'nnTTC
Cuts at: 264 737 264
Size: 473 3411
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI
BglI BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI
BsmI BsmBI BspEI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI