Restriction map for
pF1KB8432
(Circular) (Six-base) MAPSORT of: pF1KB8432.seq Check: 2948 from: 1 to: 5912
pF1KB8432 5912 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 781 781
Size: 5912
AccI GT'mk_AC
Cuts at: 1861 2138 2950 1861
Size: 277 812 4823
Fragments arranged by size:
4823 812 277
Acc65I G'GTAC_C
Cuts at: 2928 2928
Size: 5912
AclI AA'CG_TT
Cuts at: 5752 5752
Size: 5912
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 238 1243 2125 3737 4169 4522 5184 217
Size: 21 1005 882 1612 432 353 662 945
Fragments arranged by size:
1612 1005 945 882 662 432 353 21
AfeI AGC'GCT
Cuts at: 742 1792 742
Size: 1050 4862
AflIII A'CryG_T
Cuts at: 848 2713 3264 5064 5345 848
Size: 1865 551 1800 281 1415
Fragments arranged by size:
1865 1800 1415 551 281
AhdI GACnn_n'nnGTC
Cuts at: 460 1944 2237 460
Size: 1484 293 4135
Fragments arranged by size:
4135 1484 293
AleI CACnn'nnGTG
Cuts at: 1008 1008
Size: 5912
AlwNI CAG_nnn'CTG
Cuts at: 261 435 562 3217 4655 261
Size: 174 127 2655 1438 1518
Fragments arranged by size:
2655 1518 1438 174 127
ApaI G_GGCC'C
Cuts at: 1017 1045 1428 1017
Size: 28 383 5501
Fragments arranged by size:
5501 383 28
ApaLI G'TGCA_C
Cuts at: 268 1908 4750 268
Size: 1640 2842 1430
Fragments arranged by size:
2842 1640 1430
ApoI r'AATT_y
Cuts at: 2916 5265 5521 2916
Size: 2349 256 3307
Fragments arranged by size:
3307 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5912
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5912
AvaI C'yCGr_G
Cuts at: 515 551 856 1763 2932 515
Size: 36 305 907 1169 3495
Fragments arranged by size:
3495 1169 907 305 36
BamHI G'GATC_C
Cuts at: 340 2937 340
Size: 2597 3315
BanI G'GyrC_C
Cuts at: 319 865 2345 2928 3342 3590 3625 319
Size: 546 1480 583 414 248 35 2606
Fragments arranged by size:
2606 1480 583 546 414 248 35
BanII G_rGCy'C
Cuts at: 571 1017 1045 1173 1428 2363 2756 2795 2926
Size: 446 28 128 255 935 393 39 131
Cuts at: 2926 3956 571
Size: 1030 2527
Fragments arranged by size:
2527 1030 935 446 393 255 131 128
39 28
BbeI G_GCGC'C
Cuts at: 3346 3594 3346
Size: 248 5664
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 847 881 847
Size: 34 5878
BciVI GTATCCnnnnn_n'
Cuts at: 3810 4866 5912 3810
Size: 1056 1046 3810
Fragments arranged by size:
3810 1056 1046
BclI T'GATC_A
Cuts at: 3432 3432
Size: 5912
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 5912
BglII A'GATC_T
Cuts at: 2684 3427 5464 2684
Size: 743 2037 3132
Fragments arranged by size:
3132 2037 743
BlpI GC'TnA_GC
Cuts at: 3024 3024
Size: 5912
Bme1580I G_kGCm'C
Cuts at: 272 322 1017 1045 1428 1912 2348 3537 3630
Size: 50 695 28 383 484 436 1189 93
Cuts at: 3630 4754 272
Size: 1124 1430
Fragments arranged by size:
1430 1189 1124 695 484 436 383 93
50 28
BmrI ACTGGGnnnn_n'
Cuts at: 149 1671 3124 3205 3540 5684 149
Size: 1522 1453 81 335 2144 377
Fragments arranged by size:
2144 1522 1453 377 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 558 4279 558
Size: 3721 2191
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 141 151 494 625 742 1269 1981 141
Size: 10 343 131 117 527 712 4072
Fragments arranged by size:
4072 712 527 343 131 117 10
Bpu10I CC'TnA_GC
Cuts at: 744 744
Size: 5912
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 964 1157 1252 2565 2815 3088 3967 4681 4979
Size: 193 95 1313 250 273 879 714 298
Cuts at: 4979 964
Size: 1897
Fragments arranged by size:
1897 1313 879 714 298 273 250 193
95
BsaI GGTCTCn'nnnn_
Cuts at: 779 2606 5616 779
Size: 1827 3010 1075
Fragments arranged by size:
3010 1827 1075
BsaAI yAC'GTr
Cuts at: 2142 2742 3265 2142
Size: 600 523 4789
Fragments arranged by size:
4789 600 523
BsaBI GATnn'nnATC
Cuts at: 6 1186 3426 5463 6
Size: 1180 2240 2037 455
Fragments arranged by size:
2240 2037 1180 455
BsaHI Gr'CG_yC
Cuts at: 3343 3591 4293 5789 3343
Size: 248 702 1496 3466
Fragments arranged by size:
3466 1496 702 248
BsaWI w'CCGG_w
Cuts at: 221 734 2399 2435 3101 3622 4300 4711 4858
Size: 513 1665 36 666 521 678 411 147
Cuts at: 4858 5132 221
Size: 274 1001
Fragments arranged by size:
1665 1001 678 666 521 513 411 274
147 36
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1192 1530 1192
Size: 338 5574
BseYI C'CCAG_C
Cuts at: 213 1499 1559 1626 2337 2445 2865 3337 4095
Size: 1286 60 67 711 108 420 472 758
Cuts at: 4095 4760 5168 213
Size: 665 408 957
Fragments arranged by size:
1286 957 758 711 665 472 420 408
108 67 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 342 1005 1051 1360 1495 1687 1972 2200 342
Size: 663 46 309 135 192 285 228 4054
Fragments arranged by size:
4054 663 309 285 228 192 135 46
BsiEI CG_ry'CG
Cuts at: 104 222 3454 3500 4730 104
Size: 118 3232 46 1230 1286
Fragments arranged by size:
3232 1286 1230 118 46
BsiHKAI G_wGCw'C
Cuts at: 272 928 1173 1912 2363 2387 2795 2926 3704
Size: 656 245 739 451 24 408 131 778
Cuts at: 3704 3894 4754 272
Size: 190 860 1430
Fragments arranged by size:
1430 860 778 739 656 451 408 245
190 131 24
BsmI GAATG_Cn'
Cuts at: 1465 1465
Size: 5912
BsmBI CGTCTCn'nnnn_
Cuts at: 1699 2358 2485 1699
Size: 659 127 5126
Fragments arranged by size:
5126 659 127
Bsp1286I G_dGCh'C
Cuts at: 272 322 571 928 1017 1045 1173 1428 1912
Size: 50 249 357 89 28 128 255 484
Cuts at: 1912 2348 2363 2387 2756 2795 2926 3537 3630
Size: 436 15 24 369 39 131 611 93
Cuts at: 3630 3704 3894 3956 4754 272
Size: 74 190 62 798 1430
Fragments arranged by size:
1430 798 611 484 436 369 357 255
249 190 131 128 93 89 74 62
50 39 28 24 15
BspEI T'CCGG_A
Cuts at: 734 734
Size: 5912
BspHI T'CATG_A
Cuts at: 1431 2507 4344 1431
Size: 1076 1837 2999
Fragments arranged by size:
2999 1837 1076
BspMI ACCTGCnnnn'nnnn_
Cuts at: 364 547 781 1123 1517 1856 1994 2525 2962
Size: 183 234 342 394 339 138 531 437
Cuts at: 2962 3176 3478 3859 364
Size: 214 302 381 2417
Fragments arranged by size:
2417 531 437 394 381 342 339 302
234 214 183 138
BsrBI CCG'CTC
Cuts at: 50 4204 4258 5742 5907 50
Size: 4154 54 1484 165 55
Fragments arranged by size:
4154 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3824 3824
Size: 5912
BsrFI r'CCGG_y
Cuts at: 995 1070 1676 1758 3910 4091 995
Size: 75 606 82 2152 181 2816
Fragments arranged by size:
2816 2152 606 181 82 75
BsrGI T'GTAC_A
Cuts at: 845 845
Size: 5912
BssSI C'ACGA_G
Cuts at: 4183 4891 4183
Size: 708 5204
BstAPI GCAn_nnn'nTGC
Cuts at: 945 3289 945
Size: 2344 3568
BstBI TT'CG_AA
Cuts at: 1685 4273 1685
Size: 2588 3324
BstYI r'GATC_y
Cuts at: 1 340 2604 2684 2937 3404 3427 3762 4008
Size: 339 2264 80 253 467 23 335 246
Cuts at: 4008 4314 4326 4412 5464 1
Size: 306 12 86 1052 449
Fragments arranged by size:
2264 1052 467 449 339 335 306 253
246 86 80 23 12
Bsu36I CC'TnA_GG
Cuts at: 563 1211 563
Size: 648 5264
BtgI C'CryG_G
Cuts at: 1723 1749 2183 2279 2747 2762 1723
Size: 26 434 96 468 15 4873
Fragments arranged by size:
4873 468 434 96 26 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2096 2248 2373 3862 4043 4206 2096
Size: 152 125 1489 181 163 3802
Fragments arranged by size:
3802 1489 181 163 152 125
BtsI GCAGTG_nn'
Cuts at: 1912 2559 3155 1912
Size: 647 596 4669
Fragments arranged by size:
4669 647 596
ClaI AT'CG_AT
Cuts at: 953 953
Size: 5912
DraI TTT'AAA
Cuts at: 1314 2911 1314
Size: 1597 4315
DraIII CAC_nnn'GTG
Cuts at: 945 945
Size: 5912
DrdI GACnn_nn'nnGTC
Cuts at: 2404 3618 4962 2404
Size: 1214 1344 3354
Fragments arranged by size:
3354 1344 1214
EaeI y'GGCC_r
Cuts at: 239 750 780 1494 1871 2468 2593 2744 2759
Size: 511 30 714 377 597 125 151 15
Cuts at: 2759 3497 3671 4062 4089 5434 239
Size: 738 174 391 27 1345 717
Fragments arranged by size:
1345 738 717 714 597 511 391 377
174 151 125 30 27 15
EagI C'GGCC_G
Cuts at: 3497 3497
Size: 5912
EarI CTCTTCn'nnn_
Cuts at: 136 3935 4145 136
Size: 3799 210 1903
Fragments arranged by size:
3799 1903 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1294 2073 4120 4862 5008 5722 1294
Size: 779 2047 742 146 714 1484
Fragments arranged by size:
2047 1484 779 742 714 146
EcoICRI GAG'CTC
Cuts at: 1171 2361 2793 2924 1171
Size: 1190 432 131 4159
Fragments arranged by size:
4159 1190 432 131
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 141 151 217 238 494 625 742 1243 1269
Size: 10 66 21 256 131 117 501 26
Cuts at: 1269 1981 2125 3737 4169 4522 5184 141
Size: 712 144 1612 432 353 662 869
Fragments arranged by size:
1612 869 712 662 501 432 353 256
144 131 117 66 26 21 10
EcoNI CCTnn'n_nnAGG
Cuts at: 199 963 199
Size: 764 5148
EcoO109I rG'GnC_Cy
Cuts at: 210 294 444 559 968 1041 1103 1767 2231
Size: 84 150 115 409 73 62 664 464
Cuts at: 2231 3051 210
Size: 820 3071
Fragments arranged by size:
3071 820 664 464 409 150 115 84
73 62
EcoRI G'AATT_C
Cuts at: 2916 2916
Size: 5912
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 852 2580 3931 852
Size: 1728 1351 2833
Fragments arranged by size:
2833 1728 1351
FspI TGC'GCA
Cuts at: 3693 3693
Size: 5912
HaeII r_GCGC'y
Cuts at: 744 1366 1794 3346 3594 4824 5595 744
Size: 622 428 1552 248 1230 771 1061
Fragments arranged by size:
1552 1230 1061 771 622 428 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 241 273 446 478 528 560 1698 1730 1930
Size: 32 173 32 50 32 1138 32 200
Cuts at: 1930 1962 2223 2255 2603 2635 2833 2865 3754
Size: 32 261 32 348 32 198 32 889
Cuts at: 3754 3784 3786 3816 241
Size: 30 2 30 2337
Fragments arranged by size:
2337 1138 889 348 261 200 198 173
50 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2951 2951
Size: 5912
KasI G'GCGC_C
Cuts at: 3342 3590 3342
Size: 248 5664
KpnI G_GTAC'C
Cuts at: 2932 2932
Size: 5912
MluI A'CGCG_T
Cuts at: 2713 5345 2713
Size: 2632 3280
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4671 4855 4671
Size: 184 5728
MscI TGG'CCA
Cuts at: 241 752 2761 3673 241
Size: 511 2009 912 2480
Fragments arranged by size:
2480 2009 912 511
MslI CAynn'nnrTG
Cuts at: 1008 1073 1241 1390 3901 4028 1008
Size: 65 168 149 2511 127 2892
Fragments arranged by size:
2892 2511 168 149 127 65
MspA1I CmG'CkG
Cuts at: 338 687 1065 1499 1552 1671 1943 2693 2865
Size: 349 378 434 53 119 272 750 172
Cuts at: 2865 3023 3337 3697 4481 4726 338
Size: 158 314 360 784 245 1524
Fragments arranged by size:
1524 784 750 434 378 360 349 314
272 245 172 158 119 53
NaeI GCC'GGC
Cuts at: 4093 4093
Size: 5912
NarI GG'CG_CC
Cuts at: 3343 3591 3343
Size: 248 5664
NcoI C'CATG_G
Cuts at: 1723 1749 2279 1723
Size: 26 530 5356
Fragments arranged by size:
5356 530 26
NgoMIV G'CCGG_C
Cuts at: 4091 4091
Size: 5912
NspI r_CATG'y
Cuts at: 583 852 2965 3465 5068 583
Size: 269 2113 500 1603 1427
Fragments arranged by size:
2113 1603 1427 500 269
PciI A'CATG_T
Cuts at: 848 5064 848
Size: 4216 1696
PflMI CCAn_nnn'nTGG
Cuts at: 315 815 1661 315
Size: 500 846 4566
Fragments arranged by size:
4566 846 500
PfoI T'CCnGG_A
Cuts at: 397 671 698 1896 2732 397
Size: 274 27 1198 836 3577
Fragments arranged by size:
3577 1198 836 274 27
PmeI GTTT'AAAC
Cuts at: 2911 2911
Size: 5912
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 345 377 2017 2049 2489 2521 4358 4390 345
Size: 32 1640 32 440 32 1837 32 1867
Fragments arranged by size:
1867 1837 1640 440 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 210 294 444 559 968 1103 1767 2231 210
Size: 84 150 115 409 135 664 464 3891
Fragments arranged by size:
3891 664 464 409 150 135 115 84
PspOMI G'GGCC_C
Cuts at: 1013 1041 1424 1013
Size: 28 383 5501
Fragments arranged by size:
5501 383 28
PstI C_TGCA'G
Cuts at: 2959 3644 2959
Size: 685 5227
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5912
PvuII CAG'CTG
Cuts at: 687 1065 1552 1671 3337 3697 687
Size: 378 487 119 1666 360 2902
Fragments arranged by size:
2902 1666 487 378 360 119
RsrII CG'GwC_CG
Cuts at: 4107 4107
Size: 5912
SacI G_AGCT'C
Cuts at: 1173 2363 2795 2926 1173
Size: 1190 432 131 4159
Fragments arranged by size:
4159 1190 432 131
SalI G'TCGA_C
Cuts at: 2949 2949
Size: 5912
SanDI GG'GwC_CC
Cuts at: 294 294
Size: 5912
SapI GCTCTTCn'nnn_
Cuts at: 3935 4145 3935
Size: 210 5702
SbfI CC_TGCA'GG
Cuts at: 2959 2959
Size: 5912
ScaI AGT'ACT
Cuts at: 5429 5429
Size: 5912
SexAI A'CCwGG_T
Cuts at: 2813 2813
Size: 5912
SfcI C'TryA_G
Cuts at: 33 1060 1350 1638 2955 3640 4608 4799 33
Size: 1027 290 288 1317 685 968 191 1146
Fragments arranged by size:
1317 1146 1027 968 685 290 288 191
SfoI GGC'GCC
Cuts at: 3344 3592 3344
Size: 248 5664
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5912
SmaI CCC'GGG
Cuts at: 553 1765 2934 553
Size: 1212 1169 3531
Fragments arranged by size:
3531 1212 1169
SmlI C'TyrA_G
Cuts at: 856 979 1172 1267 2544 2830 3067 3982 4696
Size: 123 193 95 1277 286 237 915 714
Cuts at: 4696 4958 856
Size: 262 1810
Fragments arranged by size:
1810 1277 915 714 286 262 237 193
123 95
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5912
SphI G_CATG'C
Cuts at: 2965 3465 2965
Size: 500 5412
StyI C'CwwG_G
Cuts at: 275 344 1106 1331 1530 1655 1723 1749 1814
Size: 69 762 225 199 125 68 26 65
Cuts at: 1814 2279 2756 2777 3046 3400 275
Size: 465 477 21 269 354 2787
Fragments arranged by size:
2787 762 477 465 354 269 225 199
125 69 68 65 26 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1418 1653 2774 2812 3495 3628 4087 4296 1418
Size: 235 1121 38 683 133 459 209 3034
Fragments arranged by size:
3034 1121 683 459 235 209 133 38
TatI w'GTAC_w
Cuts at: 845 1823 5427 845
Size: 978 3604 1330
Fragments arranged by size:
3604 1330 978
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 278 310 332 364 2754 2786 5429 5461 278
Size: 32 22 32 2390 32 2643 32 729
Fragments arranged by size:
2643 2390 729 32 32 32 32 22
Tth111I GACn'n_nGTC
Cuts at: 633 3709 633
Size: 3076 2836
XbaI T'CTAG_A
Cuts at: 2943 2943
Size: 5912
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 760 816 3407 760
Size: 56 2591 3265
Fragments arranged by size:
3265 2591 56
XhoI C'TCGA_G
Cuts at: 856 856
Size: 5912
XmaI C'CCGG_G
Cuts at: 551 1763 2932 551
Size: 1212 1169 3531
Fragments arranged by size:
3531 1212 1169
XmnI GAAnn'nnTTC
Cuts at: 2032 2032
Size: 5912
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AlfI AloI AscI AvrII BaeI
BbsI BbvCI BfrBI BmgBI BmtI BsaXI BsiWI BssHII
BstEII BstXI BstZ17I CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PmlI PshAI PsiI PspXI PsrI SacII SfiI
SgrAI SnaBI SrfI SspI StuI SwaI ZraI