Restriction map for
pF1KB8434
(Circular) (Six-base) MAPSORT of: pF1KB8434.seq Check: 1014 from: 1 to: 3527
pF1KB8434 3527 bp
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
AccI GT'mk_AC
Cuts at: 565 565
Size: 3527
Acc65I G'GTAC_C
Cuts at: 497 543 497
Size: 46 3481
AclI AA'CG_TT
Cuts at: 3367 3367
Size: 3527
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1352 1784 2137 2799 1352
Size: 432 353 662 2080
Fragments arranged by size:
2080 662 432 353
AflIII A'CryG_T
Cuts at: 879 2679 2960 879
Size: 1800 281 1446
Fragments arranged by size:
1800 1446 281
AgeI A'CCGG_T
Cuts at: 354 354
Size: 3527
AleI CACnn'nnGTG
Cuts at: 394 394
Size: 3527
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 383 383
Size: 3527
AlwNI CAG_nnn'CTG
Cuts at: 207 337 832 2270 207
Size: 130 495 1438 1464
Fragments arranged by size:
1464 1438 495 130
ApaLI G'TGCA_C
Cuts at: 2365 2365
Size: 3527
ApoI r'AATT_y
Cuts at: 531 2880 3136 531
Size: 2349 256 922
Fragments arranged by size:
2349 922 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3527
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3527
AvaI C'yCGr_G
Cuts at: 371 547 371
Size: 176 3351
BamHI G'GATC_C
Cuts at: 222 552 222
Size: 330 3197
BanI G'GyrC_C
Cuts at: 497 543 957 1205 1240 497
Size: 46 414 248 35 2784
Fragments arranged by size:
2784 414 248 46 35
BanII G_rGCy'C
Cuts at: 323 541 1571 323
Size: 218 1030 2279
Fragments arranged by size:
2279 1030 218
BbeI G_GCGC'C
Cuts at: 961 1209 961
Size: 248 3279
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3493
BciVI GTATCCnnnnn_n'
Cuts at: 1425 2481 3527 1425
Size: 1056 1046 1425
Fragments arranged by size:
1425 1056 1046
BclI T'GATC_A
Cuts at: 1047 1047
Size: 3527
BglI GCCn_nnn'nGGC
Cuts at: 289 289
Size: 3527
BglII A'GATC_T
Cuts at: 1042 3079 1042
Size: 2037 1490
BlpI GC'TnA_GC
Cuts at: 639 639
Size: 3527
Bme1580I G_kGCm'C
Cuts at: 1152 1245 2369 1152
Size: 93 1124 2310
Fragments arranged by size:
2310 1124 93
BmgBI CAC'GTC
Cuts at: 429 429
Size: 3527
BmrI ACTGGGnnnn_n'
Cuts at: 128 739 820 1155 3299 128
Size: 611 81 335 2144 356
Fragments arranged by size:
2144 611 356 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1894 1894
Size: 3527
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 703 1582 2296 2594 703
Size: 879 714 298 1636
Fragments arranged by size:
1636 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3231 3231
Size: 3527
BsaAI yAC'GTr
Cuts at: 880 880
Size: 3527
BsaBI GATnn'nnATC
Cuts at: 6 1041 3078 6
Size: 1035 2037 455
Fragments arranged by size:
2037 1035 455
BsaHI Gr'CG_yC
Cuts at: 958 1206 1908 3404 958
Size: 248 702 1496 1081
Fragments arranged by size:
1496 1081 702 248
BsaWI w'CCGG_w
Cuts at: 354 716 1237 1915 2326 2473 2747 354
Size: 362 521 678 411 147 274 1134
Fragments arranged by size:
1134 678 521 411 362 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 130 484 130
Size: 354 3173
BseYI C'CCAG_C
Cuts at: 952 1710 2375 2783 952
Size: 758 665 408 1696
Fragments arranged by size:
1696 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 388
Size: 3527
BsiEI CG_ry'CG
Cuts at: 104 355 1069 1115 2345 104
Size: 251 714 46 1230 1286
Fragments arranged by size:
1286 1230 714 251 46
BsiHKAI G_wGCw'C
Cuts at: 541 1319 1509 2369 541
Size: 778 190 860 1699
Fragments arranged by size:
1699 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 323 541 1152 1245 1319 1509 1571 2369 323
Size: 218 611 93 74 190 62 798 1481
Fragments arranged by size:
1481 798 611 218 190 93 74 62
BspHI T'CATG_A
Cuts at: 266 1959 266
Size: 1693 1834
BspMI ACCTGCnnnn'nnnn_
Cuts at: 509 577 791 1093 1474 509
Size: 68 214 302 381 2562
Fragments arranged by size:
2562 381 302 214 68
BsrBI CCG'CTC
Cuts at: 50 474 1819 1873 3357 3522 50
Size: 424 1345 54 1484 165 55
Fragments arranged by size:
1484 1345 424 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1439 1439
Size: 3527
BsrFI r'CCGG_y
Cuts at: 214 354 1525 1706 214
Size: 140 1171 181 2035
Fragments arranged by size:
2035 1171 181 140
BssHII G'CGCG_C
Cuts at: 252 252
Size: 3527
BssSI C'ACGA_G
Cuts at: 1798 2506 1798
Size: 708 2819
BstAPI GCAn_nnn'nTGC
Cuts at: 337 904 337
Size: 567 2960
BstBI TT'CG_AA
Cuts at: 1888 1888
Size: 3527
BstYI r'GATC_y
Cuts at: 1 222 552 1019 1042 1377 1623 1929 1941
Size: 221 330 467 23 335 246 306 12
Cuts at: 1941 2027 3079 1
Size: 86 1052 449
Fragments arranged by size:
1052 467 449 335 330 306 246 221
86 23 12
BtgI C'CryG_G
Cuts at: 170 309 455 512 170
Size: 139 146 57 3185
Fragments arranged by size:
3185 146 139 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 164 449 1477 1658 1821 164
Size: 285 1028 181 163 1870
Fragments arranged by size:
1870 1028 285 181 163
BtsI GCAGTG_nn'
Cuts at: 770 770
Size: 3527
DraI TTT'AAA
Cuts at: 526 526
Size: 3527
DrdI GACnn_nn'nnGTC
Cuts at: 1233 2577 1233
Size: 1344 2183
EaeI y'GGCC_r
Cuts at: 161 464 1112 1286 1677 1704 3049 161
Size: 303 648 174 391 27 1345 639
Fragments arranged by size:
1345 648 639 391 303 174 27
EagI C'GGCC_G
Cuts at: 1112 1112
Size: 3527
EarI CTCTTCn'nnn_
Cuts at: 1550 1760 1550
Size: 210 3317
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 307 1735 2477 2623 3337 307
Size: 1428 742 146 714 497
Fragments arranged by size:
1428 742 714 497 146
EcoICRI GAG'CTC
Cuts at: 539 539
Size: 3527
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1352 1784 2137 2799 1352
Size: 432 353 662 2080
Fragments arranged by size:
2080 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 666 666
Size: 3527
EcoRI G'AATT_C
Cuts at: 531 531
Size: 3527
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1546 1546
Size: 3527
FspI TGC'GCA
Cuts at: 330 505 1308 330
Size: 175 803 2549
Fragments arranged by size:
2549 803 175
HaeII r_GCGC'y
Cuts at: 170 284 961 1209 2439 3210 170
Size: 114 677 248 1230 771 487
Fragments arranged by size:
1230 771 677 487 248 114
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1369 1399 1401 1431 1369
Size: 30 2 30 3465
Fragments arranged by size:
3465 30 30 2
HincII GTy'rAC
Cuts at: 237 566 237
Size: 329 3198
KasI G'GCGC_C
Cuts at: 957 1205 957
Size: 248 3279
KpnI G_GTAC'C
Cuts at: 501 547 501
Size: 46 3481
MluI A'CGCG_T
Cuts at: 2960 2960
Size: 3527
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2286 2470 2286
Size: 184 3343
MscI TGG'CCA
Cuts at: 163 1288 163
Size: 1125 2402
MslI CAynn'nnrTG
Cuts at: 271 394 1516 1643 271
Size: 123 1122 127 2155
Fragments arranged by size:
2155 1122 127 123
MspA1I CmG'CkG
Cuts at: 172 638 952 1312 2096 2341 172
Size: 466 314 360 784 245 1358
Fragments arranged by size:
1358 784 466 360 314 245
NaeI GCC'GGC
Cuts at: 216 1708 216
Size: 1492 2035
NarI GG'CG_CC
Cuts at: 958 1206 958
Size: 248 3279
NgoMIV G'CCGG_C
Cuts at: 214 1706 214
Size: 1492 2035
NspI r_CATG'y
Cuts at: 132 580 1080 2683 132
Size: 448 500 1603 976
Fragments arranged by size:
1603 976 500 448
PciI A'CATG_T
Cuts at: 2679 2679
Size: 3527
PfoI T'CCnGG_A
Cuts at: 206 383 206
Size: 177 3350
PmeI GTTT'AAAC
Cuts at: 526 526
Size: 3527
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1973 2005 1973
Size: 32 3495
PstI C_TGCA'G
Cuts at: 574 1259 574
Size: 685 2842
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3527
PvuII CAG'CTG
Cuts at: 952 1312 952
Size: 360 3167
RsrII CG'GwC_CG
Cuts at: 1722 1722
Size: 3527
SacI G_AGCT'C
Cuts at: 541 541
Size: 3527
SacII CC_GC'GG
Cuts at: 173 173
Size: 3527
SalI G'TCGA_C
Cuts at: 564 564
Size: 3527
SapI GCTCTTCn'nnn_
Cuts at: 1550 1760 1550
Size: 210 3317
SbfI CC_TGCA'GG
Cuts at: 574 574
Size: 3527
ScaI AGT'ACT
Cuts at: 3044 3044
Size: 3527
SfcI C'TryA_G
Cuts at: 33 570 1255 2223 2414 33
Size: 537 685 968 191 1146
Fragments arranged by size:
1146 968 685 537 191
SfoI GGC'GCC
Cuts at: 959 1207 959
Size: 248 3279
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3527
SmaI CCC'GGG
Cuts at: 373 549 373
Size: 176 3351
SmlI C'TyrA_G
Cuts at: 682 1597 2311 2573 682
Size: 915 714 262 1636
Fragments arranged by size:
1636 915 714 262
SpeI A'CTAG_T
Cuts at: 67 183 67
Size: 116 3411
SphI G_CATG'C
Cuts at: 132 580 1080 132
Size: 448 500 2579
Fragments arranged by size:
2579 500 448
StyI C'CwwG_G
Cuts at: 661 1015 661
Size: 354 3173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1110 1243 1702 1911 1110
Size: 133 459 209 2726
Fragments arranged by size:
2726 459 209 133
TatI w'GTAC_w
Cuts at: 3042 3042
Size: 3527
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3044 3076 3044
Size: 32 3495
Tth111I GACn'n_nGTC
Cuts at: 1324 1324
Size: 3527
XbaI T'CTAG_A
Cuts at: 558 558
Size: 3527
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1022 1022
Size: 3527
XmaI C'CCGG_G
Cuts at: 371 547 371
Size: 176 3351
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BmtI BpmI Bpu10I
BsaXI BsiWI BsmI BsmBI BspEI BsrGI BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI