Restriction map for
pF1KB8442
(Circular) (Six-base) MAPSORT of: pF1KB8442.seq Check: 3452 from: 1 to: 3491
pF1KB8442 3491 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 529 529
Size: 3491
Acc65I G'GTAC_C
Cuts at: 507 507
Size: 3491
AclI AA'CG_TT
Cuts at: 3331 3331
Size: 3491
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 1316 1748 2101 2763 283
Size: 1033 432 353 662 1011
Fragments arranged by size:
1033 1011 662 432 353
AflIII A'CryG_T
Cuts at: 843 2643 2924 843
Size: 1800 281 1410
Fragments arranged by size:
1800 1410 281
AlwNI CAG_nnn'CTG
Cuts at: 796 2234 796
Size: 1438 2053
ApaLI G'TGCA_C
Cuts at: 2329 2329
Size: 3491
ApoI r'AATT_y
Cuts at: 495 2844 3100 495
Size: 2349 256 886
Fragments arranged by size:
2349 886 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3491
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3491
AvaI C'yCGr_G
Cuts at: 200 511 200
Size: 311 3180
BamHI G'GATC_C
Cuts at: 229 516 229
Size: 287 3204
BanI G'GyrC_C
Cuts at: 507 921 1169 1204 507
Size: 414 248 35 2794
Fragments arranged by size:
2794 414 248 35
BanII G_rGCy'C
Cuts at: 505 1535 505
Size: 1030 2461
BbeI G_GCGC'C
Cuts at: 925 1173 925
Size: 248 3243
BbsI GAAGACnn'nnnn_
Cuts at: 148 148
Size: 3491
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3457
BciVI GTATCCnnnnn_n'
Cuts at: 1389 2445 3491 1389
Size: 1056 1046 1389
Fragments arranged by size:
1389 1056 1046
BclI T'GATC_A
Cuts at: 1011 1011
Size: 3491
BglII A'GATC_T
Cuts at: 1006 3043 1006
Size: 2037 1454
BlpI GC'TnA_GC
Cuts at: 603 603
Size: 3491
Bme1580I G_kGCm'C
Cuts at: 1116 1209 2333 1116
Size: 93 1124 2274
Fragments arranged by size:
2274 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 703 784 1119 3263 703
Size: 81 335 2144 931
Fragments arranged by size:
2144 931 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1858 1858
Size: 3491
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 1546 2260 2558 667
Size: 879 714 298 1600
Fragments arranged by size:
1600 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3195 3195
Size: 3491
BsaAI yAC'GTr
Cuts at: 844 844
Size: 3491
BsaBI GATnn'nnATC
Cuts at: 6 1005 3042 6
Size: 999 2037 455
Fragments arranged by size:
2037 999 455
BsaHI Gr'CG_yC
Cuts at: 922 1170 1872 3368 922
Size: 248 702 1496 1045
Fragments arranged by size:
1496 1045 702 248
BsaWI w'CCGG_w
Cuts at: 317 680 1201 1879 2290 2437 2711 317
Size: 363 521 678 411 147 274 1097
Fragments arranged by size:
1097 678 521 411 363 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 140 140
Size: 3491
BseYI C'CCAG_C
Cuts at: 213 916 1674 2339 2747 213
Size: 703 758 665 408 957
Fragments arranged by size:
957 758 703 665 408
BsiEI CG_ry'CG
Cuts at: 104 1033 1079 2309 104
Size: 929 46 1230 1286
Fragments arranged by size:
1286 1230 929 46
BsiHKAI G_wGCw'C
Cuts at: 505 1283 1473 2333 505
Size: 778 190 860 1663
Fragments arranged by size:
1663 860 778 190
BsmI GAATG_Cn'
Cuts at: 180 180
Size: 3491
Bsp1286I G_dGCh'C
Cuts at: 505 1116 1209 1283 1473 1535 2333 505
Size: 611 93 74 190 62 798 1663
Fragments arranged by size:
1663 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 1923 1923
Size: 3491
BspMI ACCTGCnnnn'nnnn_
Cuts at: 185 541 755 1057 1438 185
Size: 356 214 302 381 2238
Fragments arranged by size:
2238 381 356 302 214
BsrBI CCG'CTC
Cuts at: 50 1783 1837 3321 3486 50
Size: 1733 54 1484 165 55
Fragments arranged by size:
1733 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1403 1403
Size: 3491
BsrFI r'CCGG_y
Cuts at: 1489 1670 1489
Size: 181 3310
BssSI C'ACGA_G
Cuts at: 1762 2470 1762
Size: 708 2783
BstAPI GCAn_nnn'nTGC
Cuts at: 868 868
Size: 3491
BstBI TT'CG_AA
Cuts at: 1852 1852
Size: 3491
BstYI r'GATC_y
Cuts at: 1 229 516 983 1006 1341 1587 1893 1905
Size: 228 287 467 23 335 246 306 12
Cuts at: 1905 1991 3043 1
Size: 86 1052 449
Fragments arranged by size:
1052 467 449 335 306 287 246 228
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1441 1622 1785 1441
Size: 181 163 3147
Fragments arranged by size:
3147 181 163
BtsI GCAGTG_nn'
Cuts at: 422 734 422
Size: 312 3179
ClaI AT'CG_AT
Cuts at: 192 192
Size: 3491
DraI TTT'AAA
Cuts at: 490 490
Size: 3491
DrdI GACnn_nn'nnGTC
Cuts at: 1197 2541 1197
Size: 1344 2147
EaeI y'GGCC_r
Cuts at: 276 1076 1250 1641 1668 3013 276
Size: 800 174 391 27 1345 754
Fragments arranged by size:
1345 800 754 391 174 27
EagI C'GGCC_G
Cuts at: 1076 1076
Size: 3491
EarI CTCTTCn'nnn_
Cuts at: 359 472 1514 1724 359
Size: 113 1042 210 2126
Fragments arranged by size:
2126 1042 210 113
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 360 1699 2441 2587 3301 360
Size: 1339 742 146 714 550
Fragments arranged by size:
1339 742 714 550 146
EcoICRI GAG'CTC
Cuts at: 503 503
Size: 3491
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 1316 1748 2101 2763 283
Size: 1033 432 353 662 1011
Fragments arranged by size:
1033 1011 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 630 630
Size: 3491
EcoRI G'AATT_C
Cuts at: 495 495
Size: 3491
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1510 1510
Size: 3491
FspI TGC'GCA
Cuts at: 1272 1272
Size: 3491
HaeII r_GCGC'y
Cuts at: 336 925 1173 2403 3174 336
Size: 589 248 1230 771 653
Fragments arranged by size:
1230 771 653 589 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 135 167 1333 1363 1365 1395 135
Size: 32 1166 30 2 30 2231
Fragments arranged by size:
2231 1166 32 30 30 2
HincII GTy'rAC
Cuts at: 530 530
Size: 3491
KasI G'GCGC_C
Cuts at: 921 1169 921
Size: 248 3243
KpnI G_GTAC'C
Cuts at: 511 511
Size: 3491
MluI A'CGCG_T
Cuts at: 2924 2924
Size: 3491
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 197 338 2250 2434 197
Size: 141 1912 184 1254
Fragments arranged by size:
1912 1254 184 141
MscI TGG'CCA
Cuts at: 278 1252 278
Size: 974 2517
MslI CAynn'nnrTG
Cuts at: 1480 1607 1480
Size: 127 3364
MspA1I CmG'CkG
Cuts at: 602 916 1276 2060 2305 602
Size: 314 360 784 245 1788
Fragments arranged by size:
1788 784 360 314 245
NaeI GCC'GGC
Cuts at: 1672 1672
Size: 3491
NarI GG'CG_CC
Cuts at: 922 1170 922
Size: 248 3243
NgoMIV G'CCGG_C
Cuts at: 1670 1670
Size: 3491
NspI r_CATG'y
Cuts at: 411 544 1044 2647 411
Size: 133 500 1603 1255
Fragments arranged by size:
1603 1255 500 133
PciI A'CATG_T
Cuts at: 2643 2643
Size: 3491
PflMI CCAn_nnn'nTGG
Cuts at: 357 441 357
Size: 84 3407
PmeI GTTT'AAAC
Cuts at: 490 490
Size: 3491
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1937 1969 1937
Size: 32 3459
PstI C_TGCA'G
Cuts at: 538 1223 538
Size: 685 2806
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3491
PvuII CAG'CTG
Cuts at: 916 1276 916
Size: 360 3131
RsrII CG'GwC_CG
Cuts at: 1686 1686
Size: 3491
SacI G_AGCT'C
Cuts at: 505 505
Size: 3491
SalI G'TCGA_C
Cuts at: 528 528
Size: 3491
SapI GCTCTTCn'nnn_
Cuts at: 1514 1724 1514
Size: 210 3281
SbfI CC_TGCA'GG
Cuts at: 538 538
Size: 3491
ScaI AGT'ACT
Cuts at: 3008 3008
Size: 3491
SfcI C'TryA_G
Cuts at: 33 534 1219 2187 2378 33
Size: 501 685 968 191 1146
Fragments arranged by size:
1146 968 685 501 191
SfoI GGC'GCC
Cuts at: 923 1171 923
Size: 248 3243
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3491
SmaI CCC'GGG
Cuts at: 202 513 202
Size: 311 3180
SmlI C'TyrA_G
Cuts at: 646 1561 2275 2537 646
Size: 915 714 262 1600
Fragments arranged by size:
1600 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3491
SphI G_CATG'C
Cuts at: 544 1044 544
Size: 500 2991
StyI C'CwwG_G
Cuts at: 625 979 625
Size: 354 3137
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1074 1207 1666 1875 1074
Size: 133 459 209 2690
Fragments arranged by size:
2690 459 209 133
TatI w'GTAC_w
Cuts at: 3006 3006
Size: 3491
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3008 3040 3008
Size: 32 3459
Tth111I GACn'n_nGTC
Cuts at: 1288 1288
Size: 3491
XbaI T'CTAG_A
Cuts at: 522 522
Size: 3491
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 986 986
Size: 3491
XmaI C'CCGG_G
Cuts at: 200 511 200
Size: 311 3180
XmnI GAAnn'nnTTC
Cuts at: 127 127
Size: 3491
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI ClaI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PflMI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmBI
BspEI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI