Restriction map for
pF1KB8443
(Circular) (Six-base) MAPSORT of: pF1KB8443.seq Check: 3214 from: 1 to: 4871
pF1KB8443 4871 bp
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AccI GT'mk_AC
Cuts at: 1909 1909
Size: 4871
Acc65I G'GTAC_C
Cuts at: 1887 1887
Size: 4871
AclI AA'CG_TT
Cuts at: 4711 4711
Size: 4871
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 1436 1564 1604 1678 2696 3128 3875 4143
Size: 1048 128 40 74 1018 432 747 268
Cuts at: 4143 388
Size: 1116
Fragments arranged by size:
1116 1048 1018 747 432 268 128 74
40
AflII C'TTAA_G
Cuts at: 1677 1677
Size: 4871
AflIII A'CryG_T
Cuts at: 961 2223 3327 4304 961
Size: 1262 1104 977 1528
Fragments arranged by size:
1528 1262 1104 977
AgeI A'CCGG_T
Cuts at: 3259 3259
Size: 4871
AlwNI CAG_nnn'CTG
Cuts at: 158 436 2176 3743 158
Size: 278 1740 1567 1286
Fragments arranged by size:
1740 1567 1286 278
ApaI G_GGCC'C
Cuts at: 402 402
Size: 4871
ApaLI G'TGCA_C
Cuts at: 3641 3641
Size: 4871
ApoI r'AATT_y
Cuts at: 1061 1875 4224 4480 1061
Size: 814 2349 256 1452
Fragments arranged by size:
2349 1452 814 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4871
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4871
AvaI C'yCGr_G
Cuts at: 113 1891 113
Size: 1778 3093
BamHI G'GATC_C
Cuts at: 1644 1896 1644
Size: 252 4619
BanI G'GyrC_C
Cuts at: 527 922 1410 1822 1887 2301 2549 2584 527
Size: 395 488 412 65 414 248 35 2814
Fragments arranged by size:
2814 488 414 412 395 248 65 35
BanII G_rGCy'C
Cuts at: 292 390 402 1885 2915 292
Size: 98 12 1483 1030 2248
Fragments arranged by size:
2248 1483 1030 98 12
BbeI G_GCGC'C
Cuts at: 1414 2305 2553 1414
Size: 891 248 3732
Fragments arranged by size:
3732 891 248
BbsI GAAGACnn'nnnn_
Cuts at: 1709 1709
Size: 4871
BbvCI CC'TCA_GC
Cuts at: 492 492
Size: 4871
BciVI GTATCCnnnnn_n'
Cuts at: 1517 2769 3530 4871 1517
Size: 1252 761 1341 1517
Fragments arranged by size:
1517 1341 1252 761
BclI T'GATC_A
Cuts at: 858 2391 858
Size: 1533 3338
BglI GCCn_nnn'nGGC
Cuts at: 385 385
Size: 4871
BglII A'GATC_T
Cuts at: 1725 2386 4423 1725
Size: 661 2037 2173
Fragments arranged by size:
2173 2037 661
BlpI GC'TnA_GC
Cuts at: 134 1983 134
Size: 1849 3022
Bme1580I G_kGCm'C
Cuts at: 402 1349 2496 2589 3645 402
Size: 947 1147 93 1056 1628
Fragments arranged by size:
1628 1147 1056 947 93
BmrI ACTGGGnnnn_n'
Cuts at: 180 2083 2164 2499 4643 180
Size: 1903 81 335 2144 408
Fragments arranged by size:
2144 1903 408 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3238 3238
Size: 4871
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 237 255 1488 237
Size: 18 1233 3620
Fragments arranged by size:
3620 1233 18
Bpu10I CC'TnA_GC
Cuts at: 492 492
Size: 4871
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 1292 1517 2047 2926 3418 3716 445
Size: 847 225 530 879 492 298 1600
Fragments arranged by size:
1600 879 847 530 492 298 225
BsaI GGTCTCn'nnnn_
Cuts at: 1360 1822 4575 1360
Size: 462 2753 1656
Fragments arranged by size:
2753 1656 462
BsaAI yAC'GTr
Cuts at: 2224 2224
Size: 4871
BsaBI GATnn'nnATC
Cuts at: 6 2385 4422 6
Size: 2379 2037 455
Fragments arranged by size:
2379 2037 455
BsaHI Gr'CG_yC
Cuts at: 1411 2302 2550 3252 4748 1411
Size: 891 248 702 1496 1534
Fragments arranged by size:
1534 1496 891 702 248
BsaWI w'CCGG_w
Cuts at: 2060 2581 3259 3533 3680 4091 2060
Size: 521 678 274 147 411 2840
Fragments arranged by size:
2840 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 189 192 219 379 189
Size: 3 27 160 4681
Fragments arranged by size:
4681 160 27 3
BseYI C'CCAG_C
Cuts at: 508 1032 1441 1775 1842 2296 3054 3631 4127
Size: 524 409 334 67 454 758 577 496
Cuts at: 4127 508
Size: 1252
Fragments arranged by size:
1252 758 577 524 496 454 409 334
67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1261 1261
Size: 4871
BsiEI CG_ry'CG
Cuts at: 104 2413 2459 3667 104
Size: 2309 46 1208 1308
Fragments arranged by size:
2309 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 292 1885 2663 2853 3645 292
Size: 1593 778 190 792 1518
Fragments arranged by size:
1593 1518 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 292 390 402 1349 1885 2496 2589 2663 2853
Size: 98 12 947 536 611 93 74 190
Cuts at: 2853 2915 3645 292
Size: 62 730 1518
Fragments arranged by size:
1518 947 730 611 536 190 98 93
74 62 12
BspEI T'CCGG_A
Cuts at: 4091 4091
Size: 4871
BspHI T'CATG_A
Cuts at: 1251 1376 4047 1251
Size: 125 2671 2075
Fragments arranged by size:
2671 2075 125
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1213 1921 2135 2437 2818 1213
Size: 708 214 302 381 3266
Fragments arranged by size:
3266 708 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3163 3217 4701 4866 50
Size: 3113 54 1484 165 55
Fragments arranged by size:
3113 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 704 738 2783 704
Size: 34 2045 2792
Fragments arranged by size:
2792 2045 34
BsrFI r'CCGG_y
Cuts at: 2869 3050 3259 2869
Size: 181 209 4481
Fragments arranged by size:
4481 209 181
BssSI C'ACGA_G
Cuts at: 3142 3500 3142
Size: 358 4513
BstAPI GCAn_nnn'nTGC
Cuts at: 2248 2248
Size: 4871
BstBI TT'CG_AA
Cuts at: 3232 3232
Size: 4871
BstXI CCAn_nnnn'nTGG
Cuts at: 508 1359 1407 508
Size: 851 48 3972
Fragments arranged by size:
3972 851 48
BstYI r'GATC_y
Cuts at: 1 1644 1725 1896 2363 2386 2721 2967 3979
Size: 1643 81 171 467 23 335 246 1012
Cuts at: 3979 4065 4077 4423 1
Size: 86 12 346 449
Fragments arranged by size:
1643 1012 467 449 346 335 246 171
86 81 23 12
Bsu36I CC'TnA_GG
Cuts at: 332 347 362 875 332
Size: 15 15 513 4328
Fragments arranged by size:
4328 513 15 15
BtgI C'CryG_G
Cuts at: 171 1687 171
Size: 1516 3355
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2821 3002 3165 2821
Size: 181 163 4527
Fragments arranged by size:
4527 181 163
BtsI GCAGTG_nn'
Cuts at: 159 619 2114 159
Size: 460 1495 2916
Fragments arranged by size:
2916 1495 460
DraI TTT'AAA
Cuts at: 1870 1870
Size: 4871
DrdI GACnn_nn'nnGTC
Cuts at: 1536 2577 3435 1536
Size: 1041 858 2972
Fragments arranged by size:
2972 1041 858
EaeI y'GGCC_r
Cuts at: 438 538 2456 2630 3021 3048 4393 438
Size: 100 1918 174 391 27 1345 916
Fragments arranged by size:
1918 1345 916 391 174 100 27
EagI C'GGCC_G
Cuts at: 2456 2456
Size: 4871
EarI CTCTTCn'nnn_
Cuts at: 1307 1705 2894 3104 1307
Size: 398 1189 210 3074
Fragments arranged by size:
3074 1189 398 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3079 3389 3535 4681 3079
Size: 310 146 1146 3269
Fragments arranged by size:
3269 1146 310 146
EcoICRI GAG'CTC
Cuts at: 290 1883 290
Size: 1593 3278
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 237 255 388 1436 1488 1564 1604 1678 2696
Size: 18 133 1048 52 76 40 74 1018
Cuts at: 2696 3128 3875 4143 237
Size: 432 747 268 965
Fragments arranged by size:
1048 1018 965 747 432 268 133 76
74 52 40 18
EcoO109I rG'GnC_Cy
Cuts at: 399 688 2010 399
Size: 289 1322 3260
Fragments arranged by size:
3260 1322 289
EcoRI G'AATT_C
Cuts at: 1875 1875
Size: 4871
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 681 2890 681
Size: 2209 2662
FspI TGC'GCA
Cuts at: 2652 2652
Size: 4871
HaeII r_GCGC'y
Cuts at: 1414 1842 2305 2553 3575 4554 1414
Size: 428 463 248 1022 979 1731
Fragments arranged by size:
1731 1022 979 463 428 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 289 321 418 450 516 548 847 879 1717
Size: 32 97 32 66 32 299 32 838
Cuts at: 1717 1749 2713 2743 2745 2775 289
Size: 32 964 30 2 30 2385
Fragments arranged by size:
2385 964 838 299 97 66 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1910 1910
Size: 4871
KasI G'GCGC_C
Cuts at: 1410 2301 2549 1410
Size: 891 248 3732
Fragments arranged by size:
3732 891 248
KpnI G_GTAC'C
Cuts at: 1891 1891
Size: 4871
MluI A'CGCG_T
Cuts at: 4304 4304
Size: 4871
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 101 3542 3726 101
Size: 3441 184 1246
Fragments arranged by size:
3441 1246 184
MscI TGG'CCA
Cuts at: 440 2632 440
Size: 2192 2679
MslI CAynn'nnrTG
Cuts at: 2860 2987 2860
Size: 127 4744
MspA1I CmG'CkG
Cuts at: 1846 1982 2296 2656 3669 3914 1846
Size: 136 314 360 1013 245 2803
Fragments arranged by size:
2803 1013 360 314 245 136
NaeI GCC'GGC
Cuts at: 3052 3052
Size: 4871
NarI GG'CG_CC
Cuts at: 1411 2302 2550 1411
Size: 891 248 3732
Fragments arranged by size:
3732 891 248
NcoI C'CATG_G
Cuts at: 171 171
Size: 4871
NgoMIV G'CCGG_C
Cuts at: 3050 3050
Size: 4871
NspI r_CATG'y
Cuts at: 965 1924 2424 3331 965
Size: 959 500 907 2505
Fragments arranged by size:
2505 959 907 500
PasI CC'CwG_GG
Cuts at: 213 383 213
Size: 170 4701
PciI A'CATG_T
Cuts at: 961 3327 961
Size: 2366 2505
PmeI GTTT'AAAC
Cuts at: 1870 1870
Size: 4871
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4010 4042 4010
Size: 32 4839
PpuMI rG'GwC_Cy
Cuts at: 688 688
Size: 4871
PsiI TTA'TAA
Cuts at: 1327 1327
Size: 4871
PspOMI G'GGCC_C
Cuts at: 398 398
Size: 4871
PstI C_TGCA'G
Cuts at: 1918 2603 1918
Size: 685 4186
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4871
PvuII CAG'CTG
Cuts at: 1846 2296 2656 1846
Size: 450 360 4061
Fragments arranged by size:
4061 450 360
RsrII CG'GwC_CG
Cuts at: 3066 3066
Size: 4871
SacI G_AGCT'C
Cuts at: 292 1885 292
Size: 1593 3278
SalI G'TCGA_C
Cuts at: 1908 1908
Size: 4871
SapI GCTCTTCn'nnn_
Cuts at: 2894 3104 2894
Size: 210 4661
SbfI CC_TGCA'GG
Cuts at: 1918 1918
Size: 4871
ScaI AGT'ACT
Cuts at: 4388 4388
Size: 4871
SfcI C'TryA_G
Cuts at: 33 851 1914 2599 3592 3783 33
Size: 818 1063 685 993 191 1121
Fragments arranged by size:
1121 1063 993 818 685 191
SfoI GGC'GCC
Cuts at: 1412 2303 2551 1412
Size: 891 248 3732
Fragments arranged by size:
3732 891 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4871
SmaI CCC'GGG
Cuts at: 1893 1893
Size: 4871
SmlI C'TyrA_G
Cuts at: 424 1307 1532 1677 2026 2941 3433 3695 424
Size: 883 225 145 349 915 492 262 1600
Fragments arranged by size:
1600 915 883 492 349 262 225 145
SpeI A'CTAG_T
Cuts at: 67 1786 67
Size: 1719 3152
SphI G_CATG'C
Cuts at: 1924 2424 1924
Size: 500 4371
SspI AAT'ATT
Cuts at: 604 604
Size: 4871
StyI C'CwwG_G
Cuts at: 171 192 2005 2359 171
Size: 21 1813 354 2683
Fragments arranged by size:
2683 1813 354 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2454 2587 3046 3255 2454
Size: 133 459 209 4070
Fragments arranged by size:
4070 459 209 133
TatI w'GTAC_w
Cuts at: 4386 4386
Size: 4871
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 504 536 4388 4420 504
Size: 32 3852 32 955
Fragments arranged by size:
3852 955 32 32
Tth111I GACn'n_nGTC
Cuts at: 303 432 1621 2668 303
Size: 129 1189 1047 2506
Fragments arranged by size:
2506 1189 1047 129
XbaI T'CTAG_A
Cuts at: 1902 1902
Size: 4871
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2366 2366
Size: 4871
XmaI C'CCGG_G
Cuts at: 1891 1891
Size: 4871
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PmeI PpiI
PpuMI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsaXI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PflMI PfoI
PmlI PshAI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI