Restriction map for
pF1KB8445
(Circular) (Six-base) MAPSORT of: pF1KB8445.seq Check: 6289 from: 1 to: 4016
pF1KB8445 4016 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 663 731 663
Size: 68 3948
AccI GT'mk_AC
Cuts at: 557 1054 557
Size: 497 3519
Acc65I G'GTAC_C
Cuts at: 1032 1032
Size: 4016
AclI AA'CG_TT
Cuts at: 3856 3856
Size: 4016
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 857 1841 2273 2626 3288 857
Size: 984 432 353 662 1585
Fragments arranged by size:
1585 984 662 432 353
AfeI AGC'GCT
Cuts at: 193 997 193
Size: 804 3212
AflIII A'CryG_T
Cuts at: 1368 3168 3449 1368
Size: 1800 281 1935
Fragments arranged by size:
1935 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 597 597
Size: 4016
AlwNI CAG_nnn'CTG
Cuts at: 498 1321 2759 498
Size: 823 1438 1755
Fragments arranged by size:
1755 1438 823
ApaI G_GGCC'C
Cuts at: 117 541 117
Size: 424 3592
ApaLI G'TGCA_C
Cuts at: 2854 2854
Size: 4016
ApoI r'AATT_y
Cuts at: 1020 3369 3625 1020
Size: 2349 256 1411
Fragments arranged by size:
2349 1411 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4016
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4016
AvaI C'yCGr_G
Cuts at: 214 1036 214
Size: 822 3194
BamHI G'GATC_C
Cuts at: 1041 1041
Size: 4016
BanI G'GyrC_C
Cuts at: 1032 1446 1694 1729 1032
Size: 414 248 35 3319
Fragments arranged by size:
3319 414 248 35
BanII G_rGCy'C
Cuts at: 117 223 541 834 1030 2060 117
Size: 106 318 293 196 1030 2073
Fragments arranged by size:
2073 1030 318 293 196 106
BbeI G_GCGC'C
Cuts at: 1450 1698 1450
Size: 248 3768
BbsI GAAGACnn'nnnn_
Cuts at: 999 999
Size: 4016
BciVI GTATCCnnnnn_n'
Cuts at: 1914 2970 4016 1914
Size: 1056 1046 1914
Fragments arranged by size:
1914 1056 1046
BclI T'GATC_A
Cuts at: 1536 1536
Size: 4016
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4016
BglII A'GATC_T
Cuts at: 1531 3568 1531
Size: 2037 1979
BlpI GC'TnA_GC
Cuts at: 121 1128 121
Size: 1007 3009
Bme1580I G_kGCm'C
Cuts at: 117 541 1641 1734 2858 117
Size: 424 1100 93 1124 1275
Fragments arranged by size:
1275 1124 1100 424 93
BmrI ACTGGGnnnn_n'
Cuts at: 145 536 1228 1309 1644 3788 145
Size: 391 692 81 335 2144 373
Fragments arranged by size:
2144 692 391 373 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2383 2383
Size: 4016
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 384 497 520 793 384
Size: 113 23 273 3607
Fragments arranged by size:
3607 273 113 23
Bpu10I CC'TnA_GC
Cuts at: 767 767
Size: 4016
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1192 2071 2785 3083 1192
Size: 879 714 298 2125
Fragments arranged by size:
2125 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3720 3720
Size: 4016
BsaAI yAC'GTr
Cuts at: 978 1369 978
Size: 391 3625
BsaBI GATnn'nnATC
Cuts at: 6 1530 3567 6
Size: 1524 2037 455
Fragments arranged by size:
2037 1524 455
BsaHI Gr'CG_yC
Cuts at: 1447 1695 2397 3893 1447
Size: 248 702 1496 1570
Fragments arranged by size:
1570 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1205 1726 2404 2815 2962 3236 1205
Size: 521 678 411 147 274 1985
Fragments arranged by size:
1985 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 886 919 922 886
Size: 33 3 3980
Fragments arranged by size:
3980 33 3
BseYI C'CCAG_C
Cuts at: 116 308 1441 2199 2864 3272 116
Size: 192 1133 758 665 408 860
Fragments arranged by size:
1133 860 758 665 408 192
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 782 782
Size: 4016
BsiEI CG_ry'CG
Cuts at: 104 255 1558 1604 2834 104
Size: 151 1303 46 1230 1286
Fragments arranged by size:
1303 1286 1230 151 46
BsiHKAI G_wGCw'C
Cuts at: 146 1030 1808 1998 2858 146
Size: 884 778 190 860 1304
Fragments arranged by size:
1304 884 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 117 146 223 541 834 1030 1641 1734 1808
Size: 29 77 318 293 196 611 93 74
Cuts at: 1808 1998 2060 2858 117
Size: 190 62 798 1275
Fragments arranged by size:
1275 798 611 318 293 196 190 93
77 74 62 29
BspHI T'CATG_A
Cuts at: 176 689 2448 176
Size: 513 1759 1744
Fragments arranged by size:
1759 1744 513
BspMI ACCTGCnnnn'nnnn_
Cuts at: 663 731 1066 1280 1582 1963 663
Size: 68 335 214 302 381 2716
Fragments arranged by size:
2716 381 335 302 214 68
BsrBI CCG'CTC
Cuts at: 50 2308 2362 3846 4011 50
Size: 2258 54 1484 165 55
Fragments arranged by size:
2258 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1928 1928
Size: 4016
BsrFI r'CCGG_y
Cuts at: 2014 2195 2014
Size: 181 3835
BsrGI T'GTAC_A
Cuts at: 164 164
Size: 4016
BssSI C'ACGA_G
Cuts at: 2287 2995 2287
Size: 708 3308
BstAPI GCAn_nnn'nTGC
Cuts at: 1393 1393
Size: 4016
BstBI TT'CG_AA
Cuts at: 2377 2377
Size: 4016
BstYI r'GATC_y
Cuts at: 1 1041 1508 1531 1866 2112 2418 2430 2516
Size: 1040 467 23 335 246 306 12 86
Cuts at: 2516 3568 1
Size: 1052 449
Fragments arranged by size:
1052 1040 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 989 107
Size: 882 3134
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 419 1966 2147 2310 419
Size: 1547 181 163 2125
Fragments arranged by size:
2125 1547 181 163
BtsI GCAGTG_nn'
Cuts at: 1259 1259
Size: 4016
DraI TTT'AAA
Cuts at: 1015 1015
Size: 4016
DraIII CAC_nnn'GTG
Cuts at: 714 716 714
Size: 2 4014
DrdI GACnn_nn'nnGTC
Cuts at: 624 1722 3066 624
Size: 1098 1344 1574
Fragments arranged by size:
1574 1344 1098
EaeI y'GGCC_r
Cuts at: 333 852 1601 1775 2166 2193 3538 333
Size: 519 749 174 391 27 1345 811
Fragments arranged by size:
1345 811 749 519 391 174 27
EagI C'GGCC_G
Cuts at: 1601 1601
Size: 4016
EarI CTCTTCn'nnn_
Cuts at: 151 2039 2249 151
Size: 1888 210 1918
Fragments arranged by size:
1918 1888 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2224 2966 3112 3826 2224
Size: 742 146 714 2414
Fragments arranged by size:
2414 742 714 146
EcoICRI GAG'CTC
Cuts at: 1028 1028
Size: 4016
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 384 497 520 793 857 1841 2273 2626 3288
Size: 113 23 273 64 984 432 353 662
Cuts at: 3288 384
Size: 1112
Fragments arranged by size:
1112 984 662 432 353 273 113 64
23
EcoO109I rG'GnC_Cy
Cuts at: 113 537 538 696 1155 113
Size: 424 1 158 459 2974
Fragments arranged by size:
2974 459 424 158 1
EcoRI G'AATT_C
Cuts at: 1020 1020
Size: 4016
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 462 2035 462
Size: 1573 2443
FspI TGC'GCA
Cuts at: 1797 1797
Size: 4016
HaeII r_GCGC'y
Cuts at: 195 285 999 1450 1698 2928 3699 195
Size: 90 714 451 248 1230 771 512
Fragments arranged by size:
1230 771 714 512 451 248 90
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 583 615 1858 1888 1890 1920 583
Size: 32 1243 30 2 30 2679
Fragments arranged by size:
2679 1243 32 30 30 2
HincII GTy'rAC
Cuts at: 1055 1055
Size: 4016
KasI G'GCGC_C
Cuts at: 1446 1694 1446
Size: 248 3768
KpnI G_GTAC'C
Cuts at: 1036 1036
Size: 4016
MluI A'CGCG_T
Cuts at: 3449 3449
Size: 4016
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 609 626 2775 2959 609
Size: 17 2149 184 1666
Fragments arranged by size:
2149 1666 184 17
MscI TGG'CCA
Cuts at: 1777 1777
Size: 4016
MslI CAynn'nnrTG
Cuts at: 664 2005 2132 664
Size: 1341 127 2548
Fragments arranged by size:
2548 1341 127
MspA1I CmG'CkG
Cuts at: 240 475 915 1127 1441 1801 2585 2830 240
Size: 235 440 212 314 360 784 245 1426
Fragments arranged by size:
1426 784 440 360 314 245 235 212
NaeI GCC'GGC
Cuts at: 2197 2197
Size: 4016
NarI GG'CG_CC
Cuts at: 1447 1695 1447
Size: 248 3768
NcoI C'CATG_G
Cuts at: 107 107
Size: 4016
NdeI CA'TA_TG
Cuts at: 137 137
Size: 4016
NgoMIV G'CCGG_C
Cuts at: 2195 2195
Size: 4016
NspI r_CATG'y
Cuts at: 510 1069 1569 3172 510
Size: 559 500 1603 1354
Fragments arranged by size:
1603 1354 559 500
PciI A'CATG_T
Cuts at: 3168 3168
Size: 4016
PfoI T'CCnGG_A
Cuts at: 881 881
Size: 4016
PmeI GTTT'AAAC
Cuts at: 1015 1015
Size: 4016
PmlI CAC'GTG
Cuts at: 978 978
Size: 4016
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2462 2494 2462
Size: 32 3984
PpuMI rG'GwC_Cy
Cuts at: 696 696
Size: 4016
PspOMI G'GGCC_C
Cuts at: 113 537 113
Size: 424 3592
PstI C_TGCA'G
Cuts at: 674 1063 1748 674
Size: 389 685 2942
Fragments arranged by size:
2942 685 389
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4016
PvuII CAG'CTG
Cuts at: 1441 1801 1441
Size: 360 3656
RsrII CG'GwC_CG
Cuts at: 2211 2211
Size: 4016
SacI G_AGCT'C
Cuts at: 1030 1030
Size: 4016
SalI G'TCGA_C
Cuts at: 1053 1053
Size: 4016
SapI GCTCTTCn'nnn_
Cuts at: 151 2039 2249 151
Size: 1888 210 1918
Fragments arranged by size:
1918 1888 210
SbfI CC_TGCA'GG
Cuts at: 1063 1063
Size: 4016
ScaI AGT'ACT
Cuts at: 3533 3533
Size: 4016
SexAI A'CCwGG_T
Cuts at: 371 428 371
Size: 57 3959
SfcI C'TryA_G
Cuts at: 33 670 1059 1744 2712 2903 33
Size: 637 389 685 968 191 1146
Fragments arranged by size:
1146 968 685 637 389 191
SfoI GGC'GCC
Cuts at: 1448 1696 1448
Size: 248 3768
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4016
SmaI CCC'GGG
Cuts at: 1038 1038
Size: 4016
SmlI C'TyrA_G
Cuts at: 214 1171 2086 2800 3062 214
Size: 957 915 714 262 1168
Fragments arranged by size:
1168 957 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4016
SphI G_CATG'C
Cuts at: 510 1069 1569 510
Size: 559 500 2957
Fragments arranged by size:
2957 559 500
StyI C'CwwG_G
Cuts at: 107 1150 1504 107
Size: 1043 354 2619
Fragments arranged by size:
2619 1043 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 408 472 757 954 1599 1732 2191 2400 408
Size: 64 285 197 645 133 459 209 2024
Fragments arranged by size:
2024 645 459 285 209 197 133 64
TatI w'GTAC_w
Cuts at: 164 3531 164
Size: 3367 649
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 929 961 3533 3565 929
Size: 32 2572 32 1380
Fragments arranged by size:
2572 1380 32 32
Tth111I GACn'n_nGTC
Cuts at: 430 1813 430
Size: 1383 2633
XbaI T'CTAG_A
Cuts at: 1047 1047
Size: 4016
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 549 1511 549
Size: 962 3054
XhoI C'TCGA_G
Cuts at: 214 214
Size: 4016
XmaI C'CCGG_G
Cuts at: 1036 1036
Size: 4016
XmnI GAAnn'nnTTC
Cuts at: 184 184
Size: 4016
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PfoI PmeI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AleI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BmtI BsaXI BsiWI
BsmI BsmBI BspEI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NheI NotI NruI NsiI PacI PasI PflMI
PshAI PsiI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI ZraI