Restriction map for
pF1KB8446
(Circular) (Six-base) MAPSORT of: pF1KB8446.seq Check: 4832 from: 1 to: 4040
pF1KB8446 4040 bp
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AccI GT'mk_AC
Cuts at: 1078 1078
Size: 4040
Acc65I G'GTAC_C
Cuts at: 1056 1056
Size: 4040
AclI AA'CG_TT
Cuts at: 3880 3880
Size: 4040
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1865 2297 3044 3312 1865
Size: 432 747 268 2593
Fragments arranged by size:
2593 747 432 268
AflII C'TTAA_G
Cuts at: 256 256
Size: 4040
AflIII A'CryG_T
Cuts at: 1392 2496 3473 1392
Size: 1104 977 1959
Fragments arranged by size:
1959 1104 977
AgeI A'CCGG_T
Cuts at: 2428 2428
Size: 4040
AlwNI CAG_nnn'CTG
Cuts at: 504 1345 2912 504
Size: 841 1567 1632
Fragments arranged by size:
1632 1567 841
ApaI G_GGCC'C
Cuts at: 698 698
Size: 4040
ApaLI G'TGCA_C
Cuts at: 2810 2810
Size: 4040
ApoI r'AATT_y
Cuts at: 229 322 361 1044 3393 3649 229
Size: 93 39 683 2349 256 620
Fragments arranged by size:
2349 683 620 256 93 39
AseI AT'TA_AT
Cuts at: 20 20
Size: 4040
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
AvaI C'yCGr_G
Cuts at: 1060 1060
Size: 4040
BamHI G'GATC_C
Cuts at: 1065 1065
Size: 4040
BanI G'GyrC_C
Cuts at: 1056 1470 1718 1753 1056
Size: 414 248 35 3343
Fragments arranged by size:
3343 414 248 35
BanII G_rGCy'C
Cuts at: 698 855 915 1054 2084 698
Size: 157 60 139 1030 2654
Fragments arranged by size:
2654 1030 157 139 60
BbeI G_GCGC'C
Cuts at: 1474 1722 1474
Size: 248 3792
BbvCI CC'TCA_GC
Cuts at: 790 931 790
Size: 141 3899
BciVI GTATCCnnnnn_n'
Cuts at: 1938 2699 4040 1938
Size: 761 1341 1938
Fragments arranged by size:
1938 1341 761
BclI T'GATC_A
Cuts at: 1560 1560
Size: 4040
BglII A'GATC_T
Cuts at: 264 1555 3592 264
Size: 1291 2037 712
Fragments arranged by size:
2037 1291 712
BlpI GC'TnA_GC
Cuts at: 1152 1152
Size: 4040
Bme1580I G_kGCm'C
Cuts at: 698 1665 1758 2814 698
Size: 967 93 1056 1924
Fragments arranged by size:
1924 1056 967 93
BmrI ACTGGGnnnn_n'
Cuts at: 837 1252 1333 1668 3812 837
Size: 415 81 335 2144 1065
Fragments arranged by size:
2144 1065 415 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 4040
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 766 781 481
Size: 285 15 3740
Fragments arranged by size:
3740 285 15
Bpu10I CC'TnA_GC
Cuts at: 790 931 790
Size: 141 3899
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1216 2095 2587 2885 1216
Size: 879 492 298 2371
Fragments arranged by size:
2371 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 812 3744 812
Size: 2932 1108
BsaAI yAC'GTr
Cuts at: 1393 1393
Size: 4040
BsaBI GATnn'nnATC
Cuts at: 6 1554 3591 6
Size: 1548 2037 455
Fragments arranged by size:
2037 1548 455
BsaHI Gr'CG_yC
Cuts at: 1471 1719 2421 3917 1471
Size: 248 702 1496 1594
Fragments arranged by size:
1594 1496 702 248
BsaWI w'CCGG_w
Cuts at: 146 1229 1750 2428 2702 2849 3260 146
Size: 1083 521 678 274 147 411 926
Fragments arranged by size:
1083 926 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 643 673 643
Size: 30 4010
BseYI C'CCAG_C
Cuts at: 1465 2223 2800 3296 1465
Size: 758 577 496 2209
Fragments arranged by size:
2209 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1582 1628 2836 104
Size: 1478 46 1208 1308
Fragments arranged by size:
1478 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 915 1054 1832 2022 2814 915
Size: 139 778 190 792 2141
Fragments arranged by size:
2141 792 778 190 139
BsmI GAATG_Cn'
Cuts at: 320 320
Size: 4040
Bsp1286I G_dGCh'C
Cuts at: 698 855 915 1054 1665 1758 1832 2022 2084
Size: 157 60 139 611 93 74 190 62
Cuts at: 2084 2814 698
Size: 730 1924
Fragments arranged by size:
1924 730 611 190 157 139 93 74
62 60
BspEI T'CCGG_A
Cuts at: 3260 3260
Size: 4040
BspHI T'CATG_A
Cuts at: 3216 3216
Size: 4040
BspMI ACCTGCnnnn'nnnn_
Cuts at: 662 911 1090 1304 1606 1987 662
Size: 249 179 214 302 381 2715
Fragments arranged by size:
2715 381 302 249 214 179
BsrBI CCG'CTC
Cuts at: 50 876 2332 2386 3870 4035 50
Size: 826 1456 54 1484 165 55
Fragments arranged by size:
1484 1456 826 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1952 1952
Size: 4040
BsrFI r'CCGG_y
Cuts at: 833 2038 2219 2428 833
Size: 1205 181 209 2445
Fragments arranged by size:
2445 1205 209 181
BssSI C'ACGA_G
Cuts at: 2311 2669 2311
Size: 358 3682
BstAPI GCAn_nnn'nTGC
Cuts at: 532 954 1417 532
Size: 422 463 3155
Fragments arranged by size:
3155 463 422
BstBI TT'CG_AA
Cuts at: 2401 2401
Size: 4040
BstXI CCAn_nnnn'nTGG
Cuts at: 690 690
Size: 4040
BstYI r'GATC_y
Cuts at: 1 264 752 1065 1532 1555 1890 2136 3148
Size: 263 488 313 467 23 335 246 1012
Cuts at: 3148 3234 3246 3592 1
Size: 86 12 346 449
Fragments arranged by size:
1012 488 467 449 346 335 313 263
246 86 23 12
BtgI C'CryG_G
Cuts at: 995 995
Size: 4040
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1990 2171 2334 1990
Size: 181 163 3696
Fragments arranged by size:
3696 181 163
BtsI GCAGTG_nn'
Cuts at: 889 1283 889
Size: 394 3646
DraI TTT'AAA
Cuts at: 341 1039 341
Size: 698 3342
DraIII CAC_nnn'GTG
Cuts at: 705 705
Size: 4040
DrdI GACnn_nn'nnGTC
Cuts at: 1746 2604 1746
Size: 858 3182
EaeI y'GGCC_r
Cuts at: 938 1625 1799 2190 2217 3562 938
Size: 687 174 391 27 1345 1416
Fragments arranged by size:
1416 1345 687 391 174 27
EagI C'GGCC_G
Cuts at: 1625 1625
Size: 4040
EarI CTCTTCn'nnn_
Cuts at: 2063 2273 2063
Size: 210 3830
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 745 852 2248 2558 2704 3850 745
Size: 107 1396 310 146 1146 935
Fragments arranged by size:
1396 1146 935 310 146 107
EcoICRI GAG'CTC
Cuts at: 913 1052 913
Size: 139 3901
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 766 781 1865 2297 3044 3312 481
Size: 285 15 1084 432 747 268 1209
Fragments arranged by size:
1209 1084 747 432 285 268 15
EcoNI CCTnn'n_nnAGG
Cuts at: 940 940
Size: 4040
EcoO109I rG'GnC_Cy
Cuts at: 447 651 694 695 1179 447
Size: 204 43 1 484 3308
Fragments arranged by size:
3308 484 204 43 1
EcoRI G'AATT_C
Cuts at: 1044 1044
Size: 4040
EcoRV GAT'ATC
Cuts at: 308 308
Size: 4040
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2059 2059
Size: 4040
FspI TGC'GCA
Cuts at: 441 907 1821 441
Size: 466 914 2660
Fragments arranged by size:
2660 914 466
HaeII r_GCGC'y
Cuts at: 813 1474 1722 2744 3723 813
Size: 661 248 1022 979 1130
Fragments arranged by size:
1130 1022 979 661 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 643 675 1882 1912 1914 1944 643
Size: 32 1207 30 2 30 2739
Fragments arranged by size:
2739 1207 32 30 30 2
HincII GTy'rAC
Cuts at: 1079 1079
Size: 4040
KasI G'GCGC_C
Cuts at: 1470 1718 1470
Size: 248 3792
KpnI G_GTAC'C
Cuts at: 1060 1060
Size: 4040
MluI A'CGCG_T
Cuts at: 3473 3473
Size: 4040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 219 266 2711 2895 219
Size: 47 2445 184 1364
Fragments arranged by size:
2445 1364 184 47
MscI TGG'CCA
Cuts at: 940 1801 940
Size: 861 3179
MslI CAynn'nnrTG
Cuts at: 2029 2156 2029
Size: 127 3913
MspA1I CmG'CkG
Cuts at: 719 744 1151 1465 1825 2838 3083 719
Size: 25 407 314 360 1013 245 1676
Fragments arranged by size:
1676 1013 407 360 314 245 25
NaeI GCC'GGC
Cuts at: 2221 2221
Size: 4040
NarI GG'CG_CC
Cuts at: 1471 1719 1471
Size: 248 3792
NgoMIV G'CCGG_C
Cuts at: 2219 2219
Size: 4040
NspI r_CATG'y
Cuts at: 1093 1593 2500 1093
Size: 500 907 2633
Fragments arranged by size:
2633 907 500
PciI A'CATG_T
Cuts at: 2496 2496
Size: 4040
PflMI CCAn_nnn'nTGG
Cuts at: 504 705 504
Size: 201 3839
PfoI T'CCnGG_A
Cuts at: 779 797 779
Size: 18 4022
PmeI GTTT'AAAC
Cuts at: 1039 1039
Size: 4040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3179 3211 3179
Size: 32 4008
PpuMI rG'GwC_Cy
Cuts at: 651 651
Size: 4040
PspOMI G'GGCC_C
Cuts at: 694 694
Size: 4040
PstI C_TGCA'G
Cuts at: 473 1087 1772 473
Size: 614 685 2741
Fragments arranged by size:
2741 685 614
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4040
PvuII CAG'CTG
Cuts at: 744 1465 1825 744
Size: 721 360 2959
Fragments arranged by size:
2959 721 360
RsrII CG'GwC_CG
Cuts at: 2235 2235
Size: 4040
SacI G_AGCT'C
Cuts at: 915 1054 915
Size: 139 3901
SalI G'TCGA_C
Cuts at: 1077 1077
Size: 4040
SapI GCTCTTCn'nnn_
Cuts at: 2063 2273 2063
Size: 210 3830
SbfI CC_TGCA'GG
Cuts at: 1087 1087
Size: 4040
ScaI AGT'ACT
Cuts at: 3557 3557
Size: 4040
SfcI C'TryA_G
Cuts at: 33 174 469 1083 1768 2761 2952 33
Size: 141 295 614 685 993 191 1121
Fragments arranged by size:
1121 993 685 614 295 191 141
SfoI GGC'GCC
Cuts at: 1472 1720 1472
Size: 248 3792
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
SmaI CCC'GGG
Cuts at: 1062 1062
Size: 4040
SmlI C'TyrA_G
Cuts at: 256 1195 2110 2602 2864 256
Size: 939 915 492 262 1432
Fragments arranged by size:
1432 939 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4040
SphI G_CATG'C
Cuts at: 1093 1593 1093
Size: 500 3540
StyI C'CwwG_G
Cuts at: 689 941 1174 1528 689
Size: 252 233 354 3201
Fragments arranged by size:
3201 354 252 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1623 1756 2215 2424 1623
Size: 133 459 209 3239
Fragments arranged by size:
3239 459 209 133
TatI w'GTAC_w
Cuts at: 3555 3555
Size: 4040
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3557 3589 3557
Size: 32 4008
Tth111I GACn'n_nGTC
Cuts at: 1837 1837
Size: 4040
XbaI T'CTAG_A
Cuts at: 1071 1071
Size: 4040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 573 1535 573
Size: 962 3078
XmaI C'CCGG_G
Cuts at: 1060 1060
Size: 4040
XmnI GAAnn'nnTTC
Cuts at: 361 547 361
Size: 186 3854
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BcgI BfrBI BglI BmgBI BmtI
BseRI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI