Restriction map for
pF1KB8448
(Circular) (Six-base) MAPSORT of: pF1KB8448.seq Check: 902 from: 1 to: 4448
pF1KB8448 4448 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 262 262
Size: 4448
AccI GT'mk_AC
Cuts at: 1486 1486
Size: 4448
Acc65I G'GTAC_C
Cuts at: 1464 1464
Size: 4448
AclI AA'CG_TT
Cuts at: 4288 4288
Size: 4448
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2273 2705 3058 3720 2273
Size: 432 353 662 3001
Fragments arranged by size:
3001 662 432 353
AfeI AGC'GCT
Cuts at: 229 229
Size: 4448
AflIII A'CryG_T
Cuts at: 194 1800 3600 3881 194
Size: 1606 1800 281 761
Fragments arranged by size:
1800 1606 761 281
AleI CACnn'nnGTG
Cuts at: 256 256
Size: 4448
AlwNI CAG_nnn'CTG
Cuts at: 1753 3191 1753
Size: 1438 3010
ApaI G_GGCC'C
Cuts at: 403 771 403
Size: 368 4080
ApaLI G'TGCA_C
Cuts at: 3286 3286
Size: 4448
ApoI r'AATT_y
Cuts at: 1452 3801 4057 1452
Size: 2349 256 1843
Fragments arranged by size:
2349 1843 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4448
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4448
AvaI C'yCGr_G
Cuts at: 1157 1468 1157
Size: 311 4137
BamHI G'GATC_C
Cuts at: 1473 1473
Size: 4448
BanI G'GyrC_C
Cuts at: 384 475 613 1464 1878 2126 2161 384
Size: 91 138 851 414 248 35 2671
Fragments arranged by size:
2671 851 414 248 138 91 35
BanII G_rGCy'C
Cuts at: 403 771 1462 2492 403
Size: 368 691 1030 2359
Fragments arranged by size:
2359 1030 691 368
BbeI G_GCGC'C
Cuts at: 1882 2130 1882
Size: 248 4200
BbsI GAAGACnn'nnnn_
Cuts at: 1065 1065
Size: 4448
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 150 184 90
Size: 34 26 34 4354
Fragments arranged by size:
4354 34 34 26
BciVI GTATCCnnnnn_n'
Cuts at: 2346 3402 4448 2346
Size: 1056 1046 2346
Fragments arranged by size:
2346 1056 1046
BclI T'GATC_A
Cuts at: 1968 1968
Size: 4448
BglI GCCn_nnn'nGGC
Cuts at: 128 433 631 1297 128
Size: 305 198 666 3279
Fragments arranged by size:
3279 666 305 198
BglII A'GATC_T
Cuts at: 1963 4000 1963
Size: 2037 2411
BlpI GC'TnA_GC
Cuts at: 1560 1560
Size: 4448
Bme1580I G_kGCm'C
Cuts at: 146 403 547 771 2073 2166 3290 146
Size: 257 144 224 1302 93 1124 1304
Fragments arranged by size:
1304 1302 1124 257 224 144 93
BmrI ACTGGGnnnn_n'
Cuts at: 1205 1660 1741 2076 4220 1205
Size: 455 81 335 2144 1433
Fragments arranged by size:
2144 1433 455 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2815 2815
Size: 4448
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 964 997 1336 1378 1402 388
Size: 576 33 339 42 24 3434
Fragments arranged by size:
3434 576 339 42 33 24
Bpu10I CC'TnA_GC
Cuts at: 211 211
Size: 4448
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1624 2503 3217 3515 1624
Size: 879 714 298 2557
Fragments arranged by size:
2557 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4152 4152
Size: 4448
BsaAI yAC'GTr
Cuts at: 141 459 762 1801 141
Size: 318 303 1039 2788
Fragments arranged by size:
2788 1039 318 303
BsaBI GATnn'nnATC
Cuts at: 6 1962 3999 6
Size: 1956 2037 455
Fragments arranged by size:
2037 1956 455
BsaHI Gr'CG_yC
Cuts at: 1879 2127 2829 4325 1879
Size: 248 702 1496 2002
Fragments arranged by size:
2002 1496 702 248
BsaWI w'CCGG_w
Cuts at: 524 1349 1637 2158 2836 3247 3394 3668 524
Size: 825 288 521 678 411 147 274 1304
Fragments arranged by size:
1304 825 678 521 411 288 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 790 820 931 961 1402 1432 790
Size: 30 111 30 441 30 3806
Fragments arranged by size:
3806 441 111 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 322 553 952 1093 1276 1285 1288 1348 322
Size: 231 399 141 183 9 3 60 3422
Fragments arranged by size:
3422 399 231 183 141 60 9 3
BseYI C'CCAG_C
Cuts at: 770 1873 2631 3296 3704 770
Size: 1103 758 665 408 1514
Fragments arranged by size:
1514 1103 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1292 1990 2036 3266 104
Size: 1188 698 46 1230 1286
Fragments arranged by size:
1286 1230 1188 698 46
BsiHKAI G_wGCw'C
Cuts at: 1462 2240 2430 3290 1462
Size: 778 190 860 2620
Fragments arranged by size:
2620 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 490 490
Size: 4448
Bsp1286I G_dGCh'C
Cuts at: 146 403 547 771 1462 2073 2166 2240 2430
Size: 257 144 224 691 611 93 74 190
Cuts at: 2430 2492 3290 146
Size: 62 798 1304
Fragments arranged by size:
1304 798 691 611 257 224 190 144
93 74 62
BspEI T'CCGG_A
Cuts at: 1349 1349
Size: 4448
BspHI T'CATG_A
Cuts at: 284 605 2880 284
Size: 321 2275 1852
Fragments arranged by size:
2275 1852 321
BspMI ACCTGCnnnn'nnnn_
Cuts at: 262 740 818 1498 1712 2014 2395 262
Size: 478 78 680 214 302 381 2315
Fragments arranged by size:
2315 680 478 381 302 214 78
BsrBI CCG'CTC
Cuts at: 50 2740 2794 4278 4443 50
Size: 2690 54 1484 165 55
Fragments arranged by size:
2690 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2360 2360
Size: 4448
BsrFI r'CCGG_y
Cuts at: 1135 2446 2627 1135
Size: 1311 181 2956
Fragments arranged by size:
2956 1311 181
BssSI C'ACGA_G
Cuts at: 895 2719 3427 895
Size: 1824 708 1916
Fragments arranged by size:
1916 1824 708
BstAPI GCAn_nnn'nTGC
Cuts at: 660 707 1145 1825 660
Size: 47 438 680 3283
Fragments arranged by size:
3283 680 438 47
BstBI TT'CG_AA
Cuts at: 2809 2809
Size: 4448
BstEII G'GTnAC_C
Cuts at: 361 361
Size: 4448
BstXI CCAn_nnnn'nTGG
Cuts at: 258 258
Size: 4448
BstYI r'GATC_y
Cuts at: 1 1040 1473 1940 1963 2298 2544 2850 2862
Size: 1039 433 467 23 335 246 306 12
Cuts at: 2862 2948 4000 1
Size: 86 1052 449
Fragments arranged by size:
1052 1039 467 449 433 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 296 357 995 1292 296
Size: 61 638 297 3452
Fragments arranged by size:
3452 638 297 61
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 543 2398 2579 2742 543
Size: 1855 181 163 2249
Fragments arranged by size:
2249 1855 181 163
BtsI GCAGTG_nn'
Cuts at: 1691 1691
Size: 4448
DraI TTT'AAA
Cuts at: 1447 1447
Size: 4448
DraIII CAC_nnn'GTG
Cuts at: 750 750
Size: 4448
DrdI GACnn_nn'nnGTC
Cuts at: 2154 3498 2154
Size: 1344 3104
EaeI y'GGCC_r
Cuts at: 129 183 623 998 1031 1133 1289 1298 2033
Size: 54 440 375 33 102 156 9 735
Cuts at: 2033 2207 2598 2625 3970 129
Size: 174 391 27 1345 607
Fragments arranged by size:
1345 735 607 440 391 375 174 156
102 54 33 27 9
EagI C'GGCC_G
Cuts at: 1289 2033 1289
Size: 744 3704
EarI CTCTTCn'nnn_
Cuts at: 340 1078 1253 2471 2681 340
Size: 738 175 1218 210 2107
Fragments arranged by size:
2107 1218 738 210 175
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 165 253 434 2656 3398 3544 4258 165
Size: 88 181 2222 742 146 714 355
Fragments arranged by size:
2222 742 714 355 181 146 88
EcoICRI GAG'CTC
Cuts at: 1460 1460
Size: 4448
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 964 997 1336 1378 1402 2273 2705 3058
Size: 576 33 339 42 24 871 432 353
Cuts at: 3058 3720 388
Size: 662 1116
Fragments arranged by size:
1116 871 662 576 432 353 339 42
33 24
EcoNI CCTnn'n_nnAGG
Cuts at: 1425 1425
Size: 4448
EcoO109I rG'GnC_Cy
Cuts at: 399 828 1587 399
Size: 429 759 3260
Fragments arranged by size:
3260 759 429
EcoRI G'AATT_C
Cuts at: 1452 1452
Size: 4448
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2467 2467
Size: 4448
FspI TGC'GCA
Cuts at: 736 2229 736
Size: 1493 2955
HaeII r_GCGC'y
Cuts at: 159 231 276 1882 2130 3360 4131 159
Size: 72 45 1606 248 1230 771 476
Fragments arranged by size:
1606 1230 771 476 248 72 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 931 963 1402 1434 2290 2320 2322 2352 931
Size: 32 439 32 856 30 2 30 3027
Fragments arranged by size:
3027 856 439 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1487 1487
Size: 4448
KasI G'GCGC_C
Cuts at: 1878 2126 1878
Size: 248 4200
KpnI G_GTAC'C
Cuts at: 1468 1468
Size: 4448
MluI A'CGCG_T
Cuts at: 3881 3881
Size: 4448
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 929 3207 3391 929
Size: 2278 184 1986
Fragments arranged by size:
2278 1986 184
MscI TGG'CCA
Cuts at: 625 1033 2209 625
Size: 408 1176 2864
Fragments arranged by size:
2864 1176 408
MslI CAynn'nnrTG
Cuts at: 256 283 457 562 619 688 1000 1351 2437
Size: 27 174 105 57 69 312 351 1086
Cuts at: 2437 2564 256
Size: 127 2140
Fragments arranged by size:
2140 1086 351 312 174 127 105 69
57 27
MspA1I CmG'CkG
Cuts at: 298 549 825 1282 1294 1407 1559 1873 2233
Size: 251 276 457 12 113 152 314 360
Cuts at: 2233 3017 3262 298
Size: 784 245 1484
Fragments arranged by size:
1484 784 457 360 314 276 251 245
152 113 12
NaeI GCC'GGC
Cuts at: 1137 2629 1137
Size: 1492 2956
NarI GG'CG_CC
Cuts at: 1879 2127 1879
Size: 248 4200
NgoMIV G'CCGG_C
Cuts at: 1135 2627 1135
Size: 1492 2956
NotI GC'GGCC_GC
Cuts at: 1289 1289
Size: 4448
NspI r_CATG'y
Cuts at: 282 1392 1501 2001 3604 282
Size: 1110 109 500 1603 1126
Fragments arranged by size:
1603 1126 1110 500 109
PasI CC'CwG_GG
Cuts at: 379 379
Size: 4448
PciI A'CATG_T
Cuts at: 3600 3600
Size: 4448
PflMI CCAn_nnn'nTGG
Cuts at: 750 750
Size: 4448
PfoI T'CCnGG_A
Cuts at: 974 974
Size: 4448
PmeI GTTT'AAAC
Cuts at: 1447 1447
Size: 4448
PmlI CAC'GTG
Cuts at: 141 459 762 141
Size: 318 303 3827
Fragments arranged by size:
3827 318 303
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2894 2926 2894
Size: 32 4416
PpuMI rG'GwC_Cy
Cuts at: 828 828
Size: 4448
PspOMI G'GGCC_C
Cuts at: 399 767 399
Size: 368 4080
PstI C_TGCA'G
Cuts at: 1169 1495 2180 1169
Size: 326 685 3437
Fragments arranged by size:
3437 685 326
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4448
PvuII CAG'CTG
Cuts at: 1282 1873 2233 1282
Size: 591 360 3497
Fragments arranged by size:
3497 591 360
RsrII CG'GwC_CG
Cuts at: 2643 2643
Size: 4448
SacI G_AGCT'C
Cuts at: 1462 1462
Size: 4448
SacII CC_GC'GG
Cuts at: 299 1295 299
Size: 996 3452
SalI G'TCGA_C
Cuts at: 1485 1485
Size: 4448
SapI GCTCTTCn'nnn_
Cuts at: 1078 2471 2681 1078
Size: 1393 210 2845
Fragments arranged by size:
2845 1393 210
SbfI CC_TGCA'GG
Cuts at: 1495 1495
Size: 4448
ScaI AGT'ACT
Cuts at: 3965 3965
Size: 4448
SfcI C'TryA_G
Cuts at: 33 1165 1491 2176 3144 3335 33
Size: 1132 326 685 968 191 1146
Fragments arranged by size:
1146 1132 968 685 326 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1297 1297
Size: 4448
SfoI GGC'GCC
Cuts at: 1880 2128 1880
Size: 248 4200
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4448
SmaI CCC'GGG
Cuts at: 1159 1470 1159
Size: 311 4137
SmlI C'TyrA_G
Cuts at: 1603 2518 3232 3494 1603
Size: 915 714 262 2557
Fragments arranged by size:
2557 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4448
SphI G_CATG'C
Cuts at: 1392 1501 2001 1392
Size: 109 500 3839
Fragments arranged by size:
3839 500 109
StuI AGG'CCT
Cuts at: 1307 1307
Size: 4448
StyI C'CwwG_G
Cuts at: 1328 1582 1936 1328
Size: 254 354 3840
Fragments arranged by size:
3840 354 254
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 463 1024 2031 2164 2623 2832 463
Size: 561 1007 133 459 209 2079
Fragments arranged by size:
2079 1007 561 459 209 133
TatI w'GTAC_w
Cuts at: 197 3963 197
Size: 3766 682
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3965 3997 3965
Size: 32 4416
Tth111I GACn'n_nGTC
Cuts at: 2245 2245
Size: 4448
XbaI T'CTAG_A
Cuts at: 1479 1479
Size: 4448
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1943 1943
Size: 4448
XmaI C'CCGG_G
Cuts at: 1157 1468 1157
Size: 311 4137
XmnI GAAnn'nnTTC
Cuts at: 466 466
Size: 4448
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AhdI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI BsgI BsiWI BsmI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NruI
NsiI PacI PshAI PsiI PspXI PsrI SanDI SexAI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI