Restriction map for
pF1KB8450
(Circular) (Six-base) MAPSORT of: pF1KB8450.seq Check: 4948 from: 1 to: 5027
pF1KB8450 5027 bp
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1722 1722
Size: 5027
AccI GT'mk_AC
Cuts at: 2065 2065
Size: 5027
Acc65I G'GTAC_C
Cuts at: 2043 2043
Size: 5027
AclI AA'CG_TT
Cuts at: 4867 4867
Size: 5027
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 605 784 1366 2852 3284 4031 4299 605
Size: 179 582 1486 432 747 268 1333
Fragments arranged by size:
1486 1333 747 582 432 268 179
AfeI AGC'GCT
Cuts at: 1615 1615
Size: 5027
AflIII A'CryG_T
Cuts at: 2379 3483 4460 2379
Size: 1104 977 2946
Fragments arranged by size:
2946 1104 977
AgeI A'CCGG_T
Cuts at: 3415 3415
Size: 5027
AleI CACnn'nnGTG
Cuts at: 643 1624 643
Size: 981 4046
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1754 1966 1754
Size: 212 4815
AlwNI CAG_nnn'CTG
Cuts at: 529 1747 2332 3899 529
Size: 1218 585 1567 1657
Fragments arranged by size:
1657 1567 1218 585
ApaI G_GGCC'C
Cuts at: 1557 1557
Size: 5027
ApaLI G'TGCA_C
Cuts at: 3797 3797
Size: 5027
ApoI r'AATT_y
Cuts at: 2031 4380 4636 2031
Size: 2349 256 2422
Fragments arranged by size:
2422 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5027
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5027
AvaI C'yCGr_G
Cuts at: 2006 2047 2006
Size: 41 4986
BamHI G'GATC_C
Cuts at: 1368 2052 1368
Size: 684 4343
BanI G'GyrC_C
Cuts at: 498 2043 2457 2705 2740 498
Size: 1545 414 248 35 2785
Fragments arranged by size:
2785 1545 414 248 35
BanII G_rGCy'C
Cuts at: 1013 1557 2041 3071 1013
Size: 544 484 1030 2969
Fragments arranged by size:
2969 1030 544 484
BbeI G_GCGC'C
Cuts at: 2461 2709 2461
Size: 248 4779
BbvCI CC'TCA_GC
Cuts at: 904 904
Size: 5027
BciVI GTATCCnnnnn_n'
Cuts at: 2925 3686 5027 2925
Size: 761 1341 2925
Fragments arranged by size:
2925 1341 761
BclI T'GATC_A
Cuts at: 416 2547 416
Size: 2131 2896
BfrBI ATG'CAT
Cuts at: 436 436
Size: 5027
BglII A'GATC_T
Cuts at: 1079 2542 4579 1079
Size: 1463 2037 1527
Fragments arranged by size:
2037 1527 1463
BlpI GC'TnA_GC
Cuts at: 2139 2139
Size: 5027
Bme1580I G_kGCm'C
Cuts at: 1342 1557 2652 2745 3801 1342
Size: 215 1095 93 1056 2568
Fragments arranged by size:
2568 1095 1056 215 93
BmrI ACTGGGnnnn_n'
Cuts at: 309 2239 2320 2655 4799 309
Size: 1930 81 335 2144 537
Fragments arranged by size:
2144 1930 537 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 534 1935 3394 534
Size: 1401 1459 2167
Fragments arranged by size:
2167 1459 1401
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 742 874 952 1885 1984 156
Size: 586 132 78 933 99 3199
Fragments arranged by size:
3199 933 586 132 99 78
Bpu10I CC'TnA_GC
Cuts at: 904 904
Size: 5027
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1258 2203 3082 3574 3872 1258
Size: 945 879 492 298 2413
Fragments arranged by size:
2413 945 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1204 4731 1204
Size: 3527 1500
BsaAI yAC'GTr
Cuts at: 2380 2380
Size: 5027
BsaBI GATnn'nnATC
Cuts at: 6 673 2541 4578 6
Size: 667 1868 2037 455
Fragments arranged by size:
2037 1868 667 455
BsaHI Gr'CG_yC
Cuts at: 2458 2706 3408 4904 2458
Size: 248 702 1496 2581
Fragments arranged by size:
2581 1496 702 248
BsaWI w'CCGG_w
Cuts at: 221 665 1417 2216 2737 3415 3689 3836 4247
Size: 444 752 799 521 678 274 147 411
Cuts at: 4247 221
Size: 1001
Fragments arranged by size:
1001 799 752 678 521 444 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 361 391 811 841 961 991 361
Size: 30 420 30 120 30 4397
Fragments arranged by size:
4397 420 120 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 775 860 960 1222 1981 775
Size: 85 100 262 759 3821
Fragments arranged by size:
3821 759 262 100 85
BseYI C'CCAG_C
Cuts at: 364 994 1938 2452 3210 3787 4283 364
Size: 630 944 514 758 577 496 1108
Fragments arranged by size:
1108 944 758 630 577 514 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 469
Size: 5027
BsiEI CG_ry'CG
Cuts at: 104 1605 2569 2615 3823 104
Size: 1501 964 46 1208 1308
Fragments arranged by size:
1501 1308 1208 964 46
BsiHKAI G_wGCw'C
Cuts at: 334 1274 2041 2819 3009 3801 334
Size: 940 767 778 190 792 1560
Fragments arranged by size:
1560 940 792 778 767 190
BsmI GAATG_Cn'
Cuts at: 238 888 1885 238
Size: 650 997 3380
Fragments arranged by size:
3380 997 650
BsmBI CGTCTCn'nnnn_
Cuts at: 831 942 1675 831
Size: 111 733 4183
Fragments arranged by size:
4183 733 111
Bsp1286I G_dGCh'C
Cuts at: 334 1013 1274 1342 1557 2041 2652 2745 2819
Size: 679 261 68 215 484 611 93 74
Cuts at: 2819 3009 3071 3801 334
Size: 190 62 730 1560
Fragments arranged by size:
1560 730 679 611 484 261 215 190
93 74 68 62
BspEI T'CCGG_A
Cuts at: 1417 4247 1417
Size: 2830 2197
BspHI T'CATG_A
Cuts at: 1181 4203 1181
Size: 3022 2005
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1322 1722 2077 2291 2593 2974 1322
Size: 400 355 214 302 381 3375
Fragments arranged by size:
3375 400 381 355 302 214
BsrBI CCG'CTC
Cuts at: 50 1040 3319 3373 4857 5022 50
Size: 990 2279 54 1484 165 55
Fragments arranged by size:
2279 1484 990 165 55 54
BsrDI GCAATG_nn'
Cuts at: 161 2939 161
Size: 2778 2249
BsrFI r'CCGG_y
Cuts at: 573 3025 3206 3415 573
Size: 2452 181 209 2185
Fragments arranged by size:
2452 2185 209 181
BsrGI T'GTAC_A
Cuts at: 1292 1292
Size: 5027
BssSI C'ACGA_G
Cuts at: 3298 3656 3298
Size: 358 4669
BstAPI GCAn_nnn'nTGC
Cuts at: 2404 2404
Size: 5027
BstBI TT'CG_AA
Cuts at: 3388 3388
Size: 5027
BstYI r'GATC_y
Cuts at: 1 1079 1368 1458 1743 2052 2519 2542 2877
Size: 1078 289 90 285 309 467 23 335
Cuts at: 2877 3123 4135 4221 4233 4579 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1078 1012 467 449 346 335 309 289
285 246 90 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 958 958
Size: 5027
BtgI C'CryG_G
Cuts at: 611 1104 1163 1559 611
Size: 493 59 396 4079
Fragments arranged by size:
4079 493 396 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2977 3158 3321 2977
Size: 181 163 4683
Fragments arranged by size:
4683 181 163
BtsI GCAGTG_nn'
Cuts at: 2270 2270
Size: 5027
DraI TTT'AAA
Cuts at: 2026 2026
Size: 5027
DraIII CAC_nnn'GTG
Cuts at: 1983 1983
Size: 5027
DrdI GACnn_nn'nnGTC
Cuts at: 2733 3591 2733
Size: 858 4169
EaeI y'GGCC_r
Cuts at: 575 1844 2612 2786 3177 3204 4549 575
Size: 1269 768 174 391 27 1345 1053
Fragments arranged by size:
1345 1269 1053 768 391 174 27
EagI C'GGCC_G
Cuts at: 2612 2612
Size: 5027
EarI CTCTTCn'nnn_
Cuts at: 1535 3050 3260 1535
Size: 1515 210 3302
Fragments arranged by size:
3302 1515 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3235 3545 3691 4837 3235
Size: 310 146 1146 3425
Fragments arranged by size:
3425 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2039 2039
Size: 5027
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 605 742 784 874 952 1366 1885 1984
Size: 449 137 42 90 78 414 519 99
Cuts at: 1984 2852 3284 4031 4299 156
Size: 868 432 747 268 884
Fragments arranged by size:
884 868 747 519 449 432 414 268
137 99 90 78 42
EcoNI CCTnn'n_nnAGG
Cuts at: 1120 1120
Size: 5027
EcoO109I rG'GnC_Cy
Cuts at: 349 734 1113 1553 1554 1914 2166 349
Size: 385 379 440 1 360 252 3210
Fragments arranged by size:
3210 440 385 379 360 252 1
EcoRI G'AATT_C
Cuts at: 2031 2031
Size: 5027
EcoRV GAT'ATC
Cuts at: 1692 1692
Size: 5027
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1146 3046 1146
Size: 1900 3127
FspI TGC'GCA
Cuts at: 1249 1360 1594 2808 1249
Size: 111 234 1214 3468
Fragments arranged by size:
3468 1214 234 111
HaeII r_GCGC'y
Cuts at: 1617 2461 2709 3731 4710 1617
Size: 844 248 1022 979 1934
Fragments arranged by size:
1934 1022 979 844 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 811 843 1450 1482 1681 1713 2869 2899 2901
Size: 32 607 32 199 32 1156 30 2
Cuts at: 2901 2931 811
Size: 30 2907
Fragments arranged by size:
2907 1156 607 199 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 195 663 2066 195
Size: 468 1403 3156
Fragments arranged by size:
3156 1403 468
HindIII A'AGCT_T
Cuts at: 1789 1789
Size: 5027
KasI G'GCGC_C
Cuts at: 2457 2705 2457
Size: 248 4779
KpnI G_GTAC'C
Cuts at: 2047 2047
Size: 5027
MluI A'CGCG_T
Cuts at: 4460 4460
Size: 5027
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 653 1469 1952 3698 3882 653
Size: 816 483 1746 184 1798
Fragments arranged by size:
1798 1746 816 483 184
MscI TGG'CCA
Cuts at: 2788 2788
Size: 5027
MslI CAynn'nnrTG
Cuts at: 568 643 1265 1285 1624 3016 3143 568
Size: 75 622 20 339 1392 127 2452
Fragments arranged by size:
2452 1392 622 339 127 75 20
MspA1I CmG'CkG
Cuts at: 543 804 846 1842 1950 2138 2452 2812 3825
Size: 261 42 996 108 188 314 360 1013
Cuts at: 3825 4070 543
Size: 245 1500
Fragments arranged by size:
1500 1013 996 360 314 261 245 188
108 42
NaeI GCC'GGC
Cuts at: 575 3208 575
Size: 2633 2394
NarI GG'CG_CC
Cuts at: 2458 2706 2458
Size: 248 4779
NcoI C'CATG_G
Cuts at: 1104 1559 1104
Size: 455 4572
NdeI CA'TA_TG
Cuts at: 438 438
Size: 5027
NgoMIV G'CCGG_C
Cuts at: 573 3206 573
Size: 2633 2394
NsiI A_TGCA'T
Cuts at: 438 438
Size: 5027
NspI r_CATG'y
Cuts at: 457 879 1992 2080 2580 3487 457
Size: 422 1113 88 500 907 1997
Fragments arranged by size:
1997 1113 907 500 422 88
PasI CC'CwG_GG
Cuts at: 706 706
Size: 5027
PciI A'CATG_T
Cuts at: 3483 3483
Size: 5027
PflMI CCAn_nnn'nTGG
Cuts at: 151 837 151
Size: 686 4341
PfoI T'CCnGG_A
Cuts at: 719 719
Size: 5027
PmeI GTTT'AAAC
Cuts at: 2026 2026
Size: 5027
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 936 968 4166 4198 936
Size: 32 3198 32 1765
Fragments arranged by size:
3198 1765 32 32
PpuMI rG'GwC_Cy
Cuts at: 734 1113 734
Size: 379 4648
PshAI GACnn'nnGTC
Cuts at: 739 739
Size: 5027
PspOMI G'GGCC_C
Cuts at: 1553 1553
Size: 5027
PstI C_TGCA'G
Cuts at: 1861 1959 2074 2759 1861
Size: 98 115 685 4129
Fragments arranged by size:
4129 685 115 98
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5027
PvuII CAG'CTG
Cuts at: 543 804 846 2452 2812 543
Size: 261 42 1606 360 2758
Fragments arranged by size:
2758 1606 360 261 42
RsrII CG'GwC_CG
Cuts at: 3222 3222
Size: 5027
SacI G_AGCT'C
Cuts at: 2041 2041
Size: 5027
SalI G'TCGA_C
Cuts at: 2064 2064
Size: 5027
SanDI GG'GwC_CC
Cuts at: 734 734
Size: 5027
SapI GCTCTTCn'nnn_
Cuts at: 3050 3260 3050
Size: 210 4817
SbfI CC_TGCA'GG
Cuts at: 2074 2074
Size: 5027
ScaI AGT'ACT
Cuts at: 4544 4544
Size: 5027
SfcI C'TryA_G
Cuts at: 33 1857 1955 2070 2755 3748 3939 33
Size: 1824 98 115 685 993 191 1121
Fragments arranged by size:
1824 1121 993 685 191 115 98
SfoI GGC'GCC
Cuts at: 2459 2707 2459
Size: 248 4779
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5027
SmaI CCC'GGG
Cuts at: 2008 2049 2008
Size: 41 4986
SmlI C'TyrA_G
Cuts at: 1273 2182 3097 3589 3851 1273
Size: 909 915 492 262 2449
Fragments arranged by size:
2449 915 909 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5027
SphI G_CATG'C
Cuts at: 457 1992 2080 2580 457
Size: 1535 88 500 2904
Fragments arranged by size:
2904 1535 500 88
StyI C'CwwG_G
Cuts at: 290 920 1104 1559 2161 2515 290
Size: 630 184 455 602 354 2802
Fragments arranged by size:
2802 630 602 455 354 184
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 317 398 516 2610 2743 3202 3411 317
Size: 81 118 2094 133 459 209 1933
Fragments arranged by size:
2094 1933 459 209 133 118 81
TatI w'GTAC_w
Cuts at: 1292 1325 4542 1292
Size: 33 3217 1777
Fragments arranged by size:
3217 1777 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1806 1838 4544 4576 1806
Size: 32 2706 32 2257
Fragments arranged by size:
2706 2257 32 32
Tth111I GACn'n_nGTC
Cuts at: 823 2824 823
Size: 2001 3026
XbaI T'CTAG_A
Cuts at: 2058 2058
Size: 5027
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 176 927 2522 114
Size: 62 751 1595 2619
Fragments arranged by size:
2619 1595 751 62
XmaI C'CCGG_G
Cuts at: 2006 2047 2006
Size: 41 4986
XmnI GAAnn'nnTTC
Cuts at: 1049 1126 1792 1049
Size: 77 666 4284
Fragments arranged by size:
4284 666 77
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbvCI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BbsI
BcgI BglI BmgBI BmtI BsiWI BssHII BstEII BstXI
BstZ17I ClaI CspCI FseI FspAI HpaI MfeI NheI
NotI NruI PacI PmlI PsiI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI