Restriction map for
pF1KB8453
(Circular) (Six-base) MAPSORT of: pF1KB8453.seq Check: 5503 from: 1 to: 4076
pF1KB8453 4076 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AatII G_ACGT'C
Cuts at: 602 602
Size: 4076
AccI GT'mk_AC
Cuts at: 1114 1114
Size: 4076
Acc65I G'GTAC_C
Cuts at: 182 1092 182
Size: 910 3166
AclI AA'CG_TT
Cuts at: 3916 3916
Size: 4076
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1901 2333 2686 3348 1901
Size: 432 353 662 2629
Fragments arranged by size:
2629 662 432 353
AflIII A'CryG_T
Cuts at: 335 1428 3228 3509 335
Size: 1093 1800 281 902
Fragments arranged by size:
1800 1093 902 281
AleI CACnn'nnGTG
Cuts at: 1047 1047
Size: 4076
AlwNI CAG_nnn'CTG
Cuts at: 573 894 1381 2819 573
Size: 321 487 1438 1830
Fragments arranged by size:
1830 1438 487 321
ApaI G_GGCC'C
Cuts at: 251 979 251
Size: 728 3348
ApaLI G'TGCA_C
Cuts at: 2914 2914
Size: 4076
ApoI r'AATT_y
Cuts at: 1080 3429 3685 1080
Size: 2349 256 1471
Fragments arranged by size:
2349 1471 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4076
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4076
AvaI C'yCGr_G
Cuts at: 223 243 296 1067 1096 223
Size: 20 53 771 29 3203
Fragments arranged by size:
3203 771 53 29 20
BamHI G'GATC_C
Cuts at: 1101 1101
Size: 4076
BanI G'GyrC_C
Cuts at: 182 1063 1092 1506 1754 1789 182
Size: 881 29 414 248 35 2469
Fragments arranged by size:
2469 881 414 248 35 29
BanII G_rGCy'C
Cuts at: 144 170 251 979 1090 2120 144
Size: 26 81 728 111 1030 2100
Fragments arranged by size:
2100 1030 728 111 81 26
BbeI G_GCGC'C
Cuts at: 1510 1758 1510
Size: 248 3828
BbsI GAAGACnn'nnnn_
Cuts at: 267 295 363 267
Size: 28 68 3980
Fragments arranged by size:
3980 68 28
BbvCI CC'TCA_GC
Cuts at: 895 928 895
Size: 33 4043
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4042
BciVI GTATCCnnnnn_n'
Cuts at: 1974 3030 4076 1974
Size: 1056 1046 1974
Fragments arranged by size:
1974 1056 1046
BclI T'GATC_A
Cuts at: 1596 1596
Size: 4076
BglII A'GATC_T
Cuts at: 1591 3628 1591
Size: 2037 2039
BlpI GC'TnA_GC
Cuts at: 229 1188 229
Size: 959 3117
Bme1580I G_kGCm'C
Cuts at: 251 979 1068 1701 1794 2918 251
Size: 728 89 633 93 1124 1409
Fragments arranged by size:
1409 1124 728 633 93 89
BmrI ACTGGGnnnn_n'
Cuts at: 418 704 1288 1369 1704 3848 418
Size: 286 584 81 335 2144 646
Fragments arranged by size:
2144 646 584 335 286 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 163 1017 2443 163
Size: 854 1426 1796
Fragments arranged by size:
1796 1426 854
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 158 340 382 898 987 158
Size: 182 42 516 89 3247
Fragments arranged by size:
3247 516 182 89 42
Bpu10I CC'TnA_GC
Cuts at: 254 511 895 928 254
Size: 257 384 33 3402
Fragments arranged by size:
3402 384 257 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1252 2131 2845 3143 1252
Size: 879 714 298 2185
Fragments arranged by size:
2185 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 874 3780 874
Size: 2906 1170
BsaAI yAC'GTr
Cuts at: 338 1429 338
Size: 1091 2985
BsaBI GATnn'nnATC
Cuts at: 6 1590 3627 6
Size: 1584 2037 455
Fragments arranged by size:
2037 1584 455
BsaHI Gr'CG_yC
Cuts at: 599 1507 1755 2457 3953 599
Size: 908 248 702 1496 722
Fragments arranged by size:
1496 908 722 702 248
BsaWI w'CCGG_w
Cuts at: 1265 1786 2464 2875 3022 3296 1265
Size: 521 678 411 147 274 2045
Fragments arranged by size:
2045 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 136 1006 136
Size: 870 3206
BseYI C'CCAG_C
Cuts at: 456 616 834 853 1055 1059 1501 2259 2924
Size: 160 218 19 202 4 442 758 665
Cuts at: 2924 3332 456
Size: 408 1200
Fragments arranged by size:
1200 758 665 442 408 218 202 160
19 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 789 887 789
Size: 98 3978
BsiEI CG_ry'CG
Cuts at: 104 843 1618 1664 2894 104
Size: 739 775 46 1230 1286
Fragments arranged by size:
1286 1230 775 739 46
BsiHKAI G_wGCw'C
Cuts at: 1090 1868 2058 2918 1090
Size: 778 190 860 2248
Fragments arranged by size:
2248 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 144 170 251 979 1068 1090 1701 1794 1868
Size: 26 81 728 89 22 611 93 74
Cuts at: 1868 2058 2120 2918 144
Size: 190 62 798 1302
Fragments arranged by size:
1302 798 728 611 190 93 89 81
74 62 26 22
BspHI T'CATG_A
Cuts at: 2508 2508
Size: 4076
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1126 1340 1642 2023 1126
Size: 214 302 381 3179
Fragments arranged by size:
3179 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2368 2422 3906 4071 50
Size: 2318 54 1484 165 55
Fragments arranged by size:
2318 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 278 1988 278
Size: 1710 2366
BsrFI r'CCGG_y
Cuts at: 116 626 2074 2255 116
Size: 510 1448 181 1937
Fragments arranged by size:
1937 1448 510 181
BssSI C'ACGA_G
Cuts at: 666 2347 3055 666
Size: 1681 708 1687
Fragments arranged by size:
1687 1681 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1453 1453
Size: 4076
BstBI TT'CG_AA
Cuts at: 2437 2437
Size: 4076
BstEII G'GTnAC_C
Cuts at: 426 921 426
Size: 495 3581
BstYI r'GATC_y
Cuts at: 1 1101 1568 1591 1926 2172 2478 2490 2576
Size: 1100 467 23 335 246 306 12 86
Cuts at: 2576 3628 1
Size: 1052 449
Fragments arranged by size:
1100 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 124 440 745 809 124
Size: 316 305 64 3391
Fragments arranged by size:
3391 316 305 64
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2026 2207 2370 2026
Size: 181 163 3732
Fragments arranged by size:
3732 181 163
BtsI GCAGTG_nn'
Cuts at: 118 290 1319 118
Size: 172 1029 2875
Fragments arranged by size:
2875 1029 172
DraI TTT'AAA
Cuts at: 1075 1075
Size: 4076
DraIII CAC_nnn'GTG
Cuts at: 423 423
Size: 4076
DrdI GACnn_nn'nnGTC
Cuts at: 1782 3126 1782
Size: 1344 2732
EaeI y'GGCC_r
Cuts at: 500 840 1661 1835 2226 2253 3598 500
Size: 340 821 174 391 27 1345 978
Fragments arranged by size:
1345 978 821 391 340 174 27
EagI C'GGCC_G
Cuts at: 840 1661 840
Size: 821 3255
EarI CTCTTCn'nnn_
Cuts at: 2099 2309 2099
Size: 210 3866
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2284 3026 3172 3886 2284
Size: 742 146 714 2474
Fragments arranged by size:
2474 742 714 146
EcoICRI GAG'CTC
Cuts at: 1088 1088
Size: 4076
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 158 340 382 898 987 1901 2333 2686 3348
Size: 182 42 516 89 914 432 353 662
Cuts at: 3348 158
Size: 886
Fragments arranged by size:
914 886 662 516 432 353 182 89
42
EcoO109I rG'GnC_Cy
Cuts at: 129 218 247 248 413 975 976 996 1215
Size: 89 29 1 165 562 1 20 219
Cuts at: 1215 129
Size: 2990
Fragments arranged by size:
2990 562 219 165 89 29 20 1
1
EcoRI G'AATT_C
Cuts at: 1080 1080
Size: 4076
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2095 2095
Size: 4076
FspI TGC'GCA
Cuts at: 1857 1857
Size: 4076
HaeII r_GCGC'y
Cuts at: 562 1510 1758 2988 3759 562
Size: 948 248 1230 771 879
Fragments arranged by size:
1230 948 879 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1918 1948 1950 1980 1918
Size: 30 2 30 4014
Fragments arranged by size:
4014 30 30 2
HincII GTy'rAC
Cuts at: 1115 1115
Size: 4076
KasI G'GCGC_C
Cuts at: 1506 1754 1506
Size: 248 3828
KpnI G_GTAC'C
Cuts at: 186 1096 186
Size: 910 3166
MluI A'CGCG_T
Cuts at: 3509 3509
Size: 4076
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2835 3019 2835
Size: 184 3892
MscI TGG'CCA
Cuts at: 502 1837 502
Size: 1335 2741
MslI CAynn'nnrTG
Cuts at: 283 1047 2065 2192 283
Size: 764 1018 127 2167
Fragments arranged by size:
2167 1018 764 127
MspA1I CmG'CkG
Cuts at: 126 207 442 588 891 1059 1187 1501 1861
Size: 81 235 146 303 168 128 314 360
Cuts at: 1861 2645 2890 126
Size: 784 245 1312
Fragments arranged by size:
1312 784 360 314 303 245 235 168
146 128 81
NaeI GCC'GGC
Cuts at: 118 2257 118
Size: 2139 1937
NarI GG'CG_CC
Cuts at: 1507 1755 1507
Size: 248 3828
NcoI C'CATG_G
Cuts at: 809 809
Size: 4076
NgoMIV G'CCGG_C
Cuts at: 116 2255 116
Size: 2139 1937
NotI GC'GGCC_GC
Cuts at: 840 840
Size: 4076
NspI r_CATG'y
Cuts at: 1129 1629 3232 1129
Size: 500 1603 1973
Fragments arranged by size:
1973 1603 500
PciI A'CATG_T
Cuts at: 3228 3228
Size: 4076
PflMI CCAn_nnn'nTGG
Cuts at: 423 423
Size: 4076
PfoI T'CCnGG_A
Cuts at: 346 398 346
Size: 52 4024
PmeI GTTT'AAAC
Cuts at: 1075 1075
Size: 4076
PmlI CAC'GTG
Cuts at: 338 338
Size: 4076
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2522 2554 2522
Size: 32 4044
PpuMI rG'GwC_Cy
Cuts at: 218 413 996 218
Size: 195 583 3298
Fragments arranged by size:
3298 583 195
PshAI GACnn'nnGTC
Cuts at: 367 411 367
Size: 44 4032
PspOMI G'GGCC_C
Cuts at: 247 975 247
Size: 728 3348
PstI C_TGCA'G
Cuts at: 569 851 890 1123 1808 569
Size: 282 39 233 685 2837
Fragments arranged by size:
2837 685 282 233 39
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4076
PvuII CAG'CTG
Cuts at: 891 1059 1501 1861 891
Size: 168 442 360 3106
Fragments arranged by size:
3106 442 360 168
RsrII CG'GwC_CG
Cuts at: 2271 2271
Size: 4076
SacI G_AGCT'C
Cuts at: 1090 1090
Size: 4076
SacII CC_GC'GG
Cuts at: 127 443 127
Size: 316 3760
SalI G'TCGA_C
Cuts at: 1113 1113
Size: 4076
SanDI GG'GwC_CC
Cuts at: 218 413 218
Size: 195 3881
SapI GCTCTTCn'nnn_
Cuts at: 2099 2309 2099
Size: 210 3866
SbfI CC_TGCA'GG
Cuts at: 1123 1123
Size: 4076
ScaI AGT'ACT
Cuts at: 3593 3593
Size: 4076
SfcI C'TryA_G
Cuts at: 33 259 565 847 886 970 1119 1804 2772
Size: 226 306 282 39 84 149 685 968
Cuts at: 2772 2963 33
Size: 191 1146
Fragments arranged by size:
1146 968 685 306 282 226 191 149
84 39
SfoI GGC'GCC
Cuts at: 1508 1756 1508
Size: 248 3828
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4076
SmaI CCC'GGG
Cuts at: 245 298 1098 245
Size: 53 800 3223
Fragments arranged by size:
3223 800 53
SmlI C'TyrA_G
Cuts at: 1231 2146 2860 3122 1231
Size: 915 714 262 2185
Fragments arranged by size:
2185 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4076
SphI G_CATG'C
Cuts at: 1129 1629 1129
Size: 500 3576
StuI AGG'CCT
Cuts at: 395 395
Size: 4076
StyI C'CwwG_G
Cuts at: 553 809 1210 1564 553
Size: 256 401 354 3065
Fragments arranged by size:
3065 401 354 256
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 470 1051 1659 1792 2251 2460 470
Size: 581 608 133 459 209 2086
Fragments arranged by size:
2086 608 581 459 209 133
TatI w'GTAC_w
Cuts at: 3591 3591
Size: 4076
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3593 3625 3593
Size: 32 4044
Tth111I GACn'n_nGTC
Cuts at: 1873 1873
Size: 4076
XbaI T'CTAG_A
Cuts at: 1107 1107
Size: 4076
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1043 1571 1043
Size: 528 3548
XmaI C'CCGG_G
Cuts at: 243 296 1096 243
Size: 53 800 3223
Fragments arranged by size:
3223 800 53
ZraI GAC'GTC
Cuts at: 600 600
Size: 4076
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AlfI AloI AscI
AvrII BaeI BfrBI BglI BmgBI BmtI BsaXI BsiWI
BsmI BsmBI BspEI BsrGI BssHII BstXI BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NruI NsiI PacI PasI PsiI
PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI