Restriction map for
pF1KB8454
(Circular) (Six-base) MAPSORT of: pF1KB8454.seq Check: 3584 from: 1 to: 4712
pF1KB8454 4712 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AatII G_ACGT'C
Cuts at: 1043 1043
Size: 4712
AccI GT'mk_AC
Cuts at: 1750 1750
Size: 4712
Acc65I G'GTAC_C
Cuts at: 557 1728 557
Size: 1171 3541
AclI AA'CG_TT
Cuts at: 4552 4552
Size: 4712
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1266 1591 2537 2969 3322 3984 1266
Size: 325 946 432 353 662 1994
Fragments arranged by size:
1994 946 662 432 353 325
AflIII A'CryG_T
Cuts at: 1547 2064 3864 4145 1547
Size: 517 1800 281 2114
Fragments arranged by size:
2114 1800 517 281
AgeI A'CCGG_T
Cuts at: 1583 1583
Size: 4712
AleI CACnn'nnGTG
Cuts at: 667 667
Size: 4712
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 493 1264 1357 1568 493
Size: 771 93 211 3637
Fragments arranged by size:
3637 771 211 93
AlwNI CAG_nnn'CTG
Cuts at: 357 2017 3455 357
Size: 1660 1438 1614
Fragments arranged by size:
1660 1614 1438
ApaLI G'TGCA_C
Cuts at: 3550 3550
Size: 4712
ApoI r'AATT_y
Cuts at: 1716 4065 4321 1716
Size: 2349 256 2107
Fragments arranged by size:
2349 2107 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4712
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4712
AvaI C'yCGr_G
Cuts at: 212 292 409 495 572 1381 1660 1679 1732
Size: 80 117 86 77 809 279 19 53
Cuts at: 1732 212
Size: 3192
Fragments arranged by size:
3192 809 279 117 86 80 77 53
19
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 541 574 541
Size: 33 4679
BamHI G'GATC_C
Cuts at: 1737 1737
Size: 4712
BanI G'GyrC_C
Cuts at: 469 557 595 705 717 886 1177 1646 1728
Size: 88 38 110 12 169 291 469 82
Cuts at: 1728 2142 2390 2425 469
Size: 414 248 35 2756
Fragments arranged by size:
2756 469 414 291 248 169 110 88
82 38 35 12
BanII G_rGCy'C
Cuts at: 386 1124 1622 1726 2756 386
Size: 738 498 104 1030 2342
Fragments arranged by size:
2342 1030 738 498 104
BbeI G_GCGC'C
Cuts at: 599 1650 2146 2394 599
Size: 1051 496 248 2917
Fragments arranged by size:
2917 1051 496 248
BbsI GAAGACnn'nnnn_
Cuts at: 915 1035 915
Size: 120 4592
BbvCI CC'TCA_GC
Cuts at: 229 229
Size: 4712
BciVI GTATCCnnnnn_n'
Cuts at: 2610 3666 4712 2610
Size: 1056 1046 2610
Fragments arranged by size:
2610 1056 1046
BclI T'GATC_A
Cuts at: 2232 2232
Size: 4712
BglI GCCn_nnn'nGGC
Cuts at: 632 636 632
Size: 4 4708
BglII A'GATC_T
Cuts at: 2227 4264 2227
Size: 2037 2675
BlpI GC'TnA_GC
Cuts at: 1824 1824
Size: 4712
Bme1580I G_kGCm'C
Cuts at: 662 710 1180 2337 2430 3554 662
Size: 48 470 1157 93 1124 1820
Fragments arranged by size:
1820 1157 1124 470 93 48
BmgBI CAC'GTC
Cuts at: 774 798 774
Size: 24 4688
BmrI ACTGGGnnnn_n'
Cuts at: 1924 2005 2340 4484 1924
Size: 81 335 2144 2152
Fragments arranged by size:
2152 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3079 3079
Size: 4712
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 1204 502
Size: 702 4010
Bpu10I CC'TnA_GC
Cuts at: 229 565 229
Size: 336 4376
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 844 1081 1888 2767 3481 3779 844
Size: 237 807 879 714 298 1777
Fragments arranged by size:
1777 879 807 714 298 237
BsaI GGTCTCn'nnnn_
Cuts at: 4416 4416
Size: 4712
BsaAI yAC'GTr
Cuts at: 2065 2065
Size: 4712
BsaBI GATnn'nnATC
Cuts at: 6 2226 4263 6
Size: 2220 2037 455
Fragments arranged by size:
2220 2037 455
BsaHI Gr'CG_yC
Cuts at: 452 596 806 1007 1040 1085 1647 2143 2391
Size: 144 210 201 33 45 562 496 248
Cuts at: 2391 3093 4589 452
Size: 702 1496 575
Fragments arranged by size:
1496 702 575 562 496 248 210 201
144 45 33
BsaWI w'CCGG_w
Cuts at: 1583 1901 2422 3100 3511 3658 3932 1583
Size: 318 521 678 411 147 274 2363
Fragments arranged by size:
2363 678 521 411 318 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 607 637 1177 1207 607
Size: 30 540 30 4112
Fragments arranged by size:
4112 540 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 394 523 525 394
Size: 129 2 4581
Fragments arranged by size:
4581 129 2
BseYI C'CCAG_C
Cuts at: 364 646 789 1329 1433 1537 2137 2895 3560
Size: 282 143 540 104 104 600 758 665
Cuts at: 3560 3968 364
Size: 408 1108
Fragments arranged by size:
1108 758 665 600 540 408 282 143
104 104
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 691 1185 1327 1339 160
Size: 531 494 142 12 3533
Fragments arranged by size:
3533 531 494 142 12
BsiEI CG_ry'CG
Cuts at: 104 377 505 856 951 1584 2254 2300 3530
Size: 273 128 351 95 633 670 46 1230
Cuts at: 3530 104
Size: 1286
Fragments arranged by size:
1286 1230 670 633 351 273 128 95
46
BsiHKAI G_wGCw'C
Cuts at: 386 917 1547 1630 1726 2504 2694 3554 386
Size: 531 630 83 96 778 190 860 1544
Fragments arranged by size:
1544 860 778 630 531 190 96 83
BsmBI CGTCTCn'nnnn_
Cuts at: 111 1014 111
Size: 903 3809
Bsp1286I G_dGCh'C
Cuts at: 386 662 710 917 1124 1180 1547 1622 1630
Size: 276 48 207 207 56 367 75 8
Cuts at: 1630 1726 2337 2430 2504 2694 2756 3554 386
Size: 96 611 93 74 190 62 798 1544
Fragments arranged by size:
1544 798 611 367 276 207 207 190
96 93 75 74 62 56 48 8
BspHI T'CATG_A
Cuts at: 3144 3144
Size: 4712
BspMI ACCTGCnnnn'nnnn_
Cuts at: 320 692 773 989 1034 1574 1604 1762 1976
Size: 372 81 216 45 540 30 158 214
Cuts at: 1976 2278 2659 320
Size: 302 381 2373
Fragments arranged by size:
2373 540 381 372 302 216 214 158
81 45 30
BsrBI CCG'CTC
Cuts at: 50 699 858 3004 3058 4542 4707 50
Size: 649 159 2146 54 1484 165 55
Fragments arranged by size:
2146 1484 649 165 159 55 54
BsrDI GCAATG_nn'
Cuts at: 2624 2624
Size: 4712
BsrFI r'CCGG_y
Cuts at: 151 445 950 1303 1351 1583 2710 2891 151
Size: 294 505 353 48 232 1127 181 1972
Fragments arranged by size:
1972 1127 505 353 294 232 181 48
BssSI C'ACGA_G
Cuts at: 754 784 1402 2983 3691 754
Size: 30 618 1581 708 1775
Fragments arranged by size:
1775 1581 708 618 30
BstAPI GCAn_nnn'nTGC
Cuts at: 357 2089 357
Size: 1732 2980
BstBI TT'CG_AA
Cuts at: 3073 3073
Size: 4712
BstXI CCAn_nnnn'nTGG
Cuts at: 480 480
Size: 4712
BstYI r'GATC_y
Cuts at: 1 1067 1737 2204 2227 2562 2808 3114 3126
Size: 1066 670 467 23 335 246 306 12
Cuts at: 3126 3212 4264 1
Size: 86 1052 449
Fragments arranged by size:
1066 1052 670 467 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 518 518
Size: 4712
BtgI C'CryG_G
Cuts at: 850 1473 850
Size: 623 4089
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2662 2843 3006 2662
Size: 181 163 4368
Fragments arranged by size:
4368 181 163
BtsI GCAGTG_nn'
Cuts at: 1229 1327 1359 1955 1229
Size: 98 32 596 3986
Fragments arranged by size:
3986 596 98 32
DraI TTT'AAA
Cuts at: 1711 1711
Size: 4712
DraIII CAC_nnn'GTG
Cuts at: 393 912 393
Size: 519 4193
DrdI GACnn_nn'nnGTC
Cuts at: 2418 3762 2418
Size: 1344 3368
EaeI y'GGCC_r
Cuts at: 149 374 502 547 853 948 1305 2297 2471
Size: 225 128 45 306 95 357 992 174
Cuts at: 2471 2862 2889 4234 149
Size: 391 27 1345 627
Fragments arranged by size:
1345 992 627 391 357 306 225 174
128 95 45 27
EagI C'GGCC_G
Cuts at: 374 502 853 948 2297 374
Size: 128 351 95 1349 2789
Fragments arranged by size:
2789 1349 351 128 95
EarI CTCTTCn'nnn_
Cuts at: 2735 2945 2735
Size: 210 4502
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 892 1234 1398 1714 2920 3662 3808 4522 892
Size: 342 164 316 1206 742 146 714 1082
Fragments arranged by size:
1206 1082 742 714 342 316 164 146
EcoICRI GAG'CTC
Cuts at: 384 1724 384
Size: 1340 3372
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 1204 1266 1591 2537 2969 3322 3984 502
Size: 702 62 325 946 432 353 662 1230
Fragments arranged by size:
1230 946 702 662 432 353 325 62
EcoNI CCTnn'n_nnAGG
Cuts at: 997 997
Size: 4712
EcoO109I rG'GnC_Cy
Cuts at: 304 1458 1657 1851 304
Size: 1154 199 194 3165
Fragments arranged by size:
3165 1154 199 194
EcoRI G'AATT_C
Cuts at: 1716 1716
Size: 4712
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2731 2731
Size: 4712
FseI GG_CCGG'CC
Cuts at: 155 155
Size: 4712
FspI TGC'GCA
Cuts at: 436 985 1021 1141 2493 436
Size: 549 36 120 1352 2655
Fragments arranged by size:
2655 1352 549 120 36
FspAI rTGC'GCAy
Cuts at: 436 436
Size: 4712
HaeII r_GCGC'y
Cuts at: 572 599 1650 2146 2394 3624 4395 572
Size: 27 1051 496 248 1230 771 889
Fragments arranged by size:
1230 1051 889 771 496 248 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 994 1026 1177 1209 2554 2584 2586 2616 994
Size: 32 151 32 1345 30 2 30 3090
Fragments arranged by size:
3090 1345 151 32 32 30 30 2
HincII GTy'rAC
Cuts at: 750 1751 750
Size: 1001 3711
KasI G'GCGC_C
Cuts at: 595 1646 2142 2390 595
Size: 1051 496 248 2917
Fragments arranged by size:
2917 1051 496 248
KpnI G_GTAC'C
Cuts at: 561 1732 561
Size: 1171 3541
MluI A'CGCG_T
Cuts at: 4145 4145
Size: 4712
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 704 851 3471 3655 704
Size: 147 2620 184 1761
Fragments arranged by size:
2620 1761 184 147
MscI TGG'CCA
Cuts at: 2473 2473
Size: 4712
MslI CAynn'nnrTG
Cuts at: 112 475 667 1315 1550 2701 2828 112
Size: 363 192 648 235 1151 127 1996
Fragments arranged by size:
1996 1151 648 363 235 192 127
MspA1I CmG'CkG
Cuts at: 357 650 852 1475 1823 2137 2497 3281 3526
Size: 293 202 623 348 314 360 784 245
Cuts at: 3526 357
Size: 1543
Fragments arranged by size:
1543 784 623 360 348 314 293 245
202
NaeI GCC'GGC
Cuts at: 153 447 952 1353 2893 153
Size: 294 505 401 1540 1972
Fragments arranged by size:
1972 1540 505 401 294
NarI GG'CG_CC
Cuts at: 596 1647 2143 2391 596
Size: 1051 496 248 2917
Fragments arranged by size:
2917 1051 496 248
NgoMIV G'CCGG_C
Cuts at: 151 445 950 1351 2891 151
Size: 294 505 401 1540 1972
Fragments arranged by size:
1972 1540 505 401 294
NotI GC'GGCC_GC
Cuts at: 853 853
Size: 4712
NspI r_CATG'y
Cuts at: 1140 1215 1551 1765 2265 3868 1140
Size: 75 336 214 500 1603 1984
Fragments arranged by size:
1984 1603 500 336 214 75
PasI CC'CwG_GG
Cuts at: 710 710
Size: 4712
PciI A'CATG_T
Cuts at: 1547 3864 1547
Size: 2317 2395
PflMI CCAn_nnn'nTGG
Cuts at: 393 789 1314 393
Size: 396 525 3791
Fragments arranged by size:
3791 525 396
PfoI T'CCnGG_A
Cuts at: 866 926 866
Size: 60 4652
PmeI GTTT'AAAC
Cuts at: 1711 1711
Size: 4712
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3158 3190 3158
Size: 32 4680
PpuMI rG'GwC_Cy
Cuts at: 1458 1657 1458
Size: 199 4513
PstI C_TGCA'G
Cuts at: 353 1220 1533 1759 2444 353
Size: 867 313 226 685 2621
Fragments arranged by size:
2621 867 685 313 226
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4712
PvuII CAG'CTG
Cuts at: 650 2137 2497 650
Size: 1487 360 2865
Fragments arranged by size:
2865 1487 360
RsrII CG'GwC_CG
Cuts at: 2907 2907
Size: 4712
SacI G_AGCT'C
Cuts at: 386 1726 386
Size: 1340 3372
SacII CC_GC'GG
Cuts at: 853 1476 853
Size: 623 4089
SalI G'TCGA_C
Cuts at: 1749 1749
Size: 4712
SanDI GG'GwC_CC
Cuts at: 1657 1657
Size: 4712
SapI GCTCTTCn'nnn_
Cuts at: 2735 2945 2735
Size: 210 4502
SbfI CC_TGCA'GG
Cuts at: 1759 1759
Size: 4712
ScaI AGT'ACT
Cuts at: 676 1264 4229 676
Size: 588 2965 1159
Fragments arranged by size:
2965 1159 588
SexAI A'CCwGG_T
Cuts at: 239 239
Size: 4712
SfcI C'TryA_G
Cuts at: 33 349 1216 1529 1755 2440 3408 3599 33
Size: 316 867 313 226 685 968 191 1146
Fragments arranged by size:
1146 968 867 685 316 313 226 191
SfoI GGC'GCC
Cuts at: 597 1648 2144 2392 597
Size: 1051 496 248 2917
Fragments arranged by size:
2917 1051 496 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4712
SmaI CCC'GGG
Cuts at: 497 574 1681 1734 497
Size: 77 1107 53 3475
Fragments arranged by size:
3475 1107 77 53
SmlI C'TyrA_G
Cuts at: 859 1096 1867 2782 3496 3758 859
Size: 237 771 915 714 262 1813
Fragments arranged by size:
1813 915 771 714 262 237
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4712
SphI G_CATG'C
Cuts at: 1765 2265 1765
Size: 500 4212
StyI C'CwwG_G
Cuts at: 440 692 1846 2200 440
Size: 252 1154 354 2952
Fragments arranged by size:
2952 1154 354 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 600 1012 1132 2295 2428 2887 3096 600
Size: 412 120 1163 133 459 209 2216
Fragments arranged by size:
2216 1163 459 412 209 133 120
TatI w'GTAC_w
Cuts at: 674 896 1262 4227 674
Size: 222 366 2965 1159
Fragments arranged by size:
2965 1159 366 222
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 607 639 4229 4261 607
Size: 32 3590 32 1058
Fragments arranged by size:
3590 1058 32 32
Tth111I GACn'n_nGTC
Cuts at: 192 1006 1039 1084 2509 192
Size: 814 33 45 1425 2395
Fragments arranged by size:
2395 1425 814 45 33
XbaI T'CTAG_A
Cuts at: 1743 1743
Size: 4712
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 699 2207 699
Size: 1508 3204
XmaI C'CCGG_G
Cuts at: 495 572 1679 1732 495
Size: 77 1107 53 3475
Fragments arranged by size:
3475 1107 77 53
ZraI GAC'GTC
Cuts at: 1041 1041
Size: 4712
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI FspAI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AloI ApaI AscI AvrII
BcgI BfrBI BmtI BsiWI BsmI BspEI BsrGI BssHII
BstEII BstZ17I ClaI CspCI EcoRV HindIII HpaI MfeI
NcoI NdeI NheI NruI NsiI PacI PmlI PshAI
PsiI PspOMI PspXI PsrI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI