Restriction map for
pF1KB8456
(Circular) (Six-base) MAPSORT of: pF1KB8456.seq Check: 4231 from: 1 to: 4568
pF1KB8456 4568 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AccI GT'mk_AC
Cuts at: 1606 1606
Size: 4568
Acc65I G'GTAC_C
Cuts at: 1584 1584
Size: 4568
AclI AA'CG_TT
Cuts at: 4408 4408
Size: 4568
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 977 1145 1307 2393 2825 3178 3840 353
Size: 624 168 162 1086 432 353 662 1081
Fragments arranged by size:
1086 1081 662 624 432 353 168 162
AflIII A'CryG_T
Cuts at: 1920 3720 4001 1920
Size: 1800 281 2487
Fragments arranged by size:
2487 1800 281
AleI CACnn'nnGTG
Cuts at: 1195 1195
Size: 4568
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 959 959
Size: 4568
AlwNI CAG_nnn'CTG
Cuts at: 450 554 1873 3311 450
Size: 104 1319 1438 1707
Fragments arranged by size:
1707 1438 1319 104
ApaLI G'TGCA_C
Cuts at: 1030 3406 1030
Size: 2376 2192
ApoI r'AATT_y
Cuts at: 269 326 1572 3921 4177 269
Size: 57 1246 2349 256 660
Fragments arranged by size:
2349 1246 660 256 57
AseI AT'TA_AT
Cuts at: 20 20
Size: 4568
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4568
AvaI C'yCGr_G
Cuts at: 1054 1588 1054
Size: 534 4034
BamHI G'GATC_C
Cuts at: 951 1593 951
Size: 642 3926
BanI G'GyrC_C
Cuts at: 570 1050 1584 1998 2246 2281 570
Size: 480 534 414 248 35 2857
Fragments arranged by size:
2857 534 480 414 248 35
BanII G_rGCy'C
Cuts at: 1582 2612 1582
Size: 1030 3538
BbeI G_GCGC'C
Cuts at: 2002 2250 2002
Size: 248 4320
BbsI GAAGACnn'nnnn_
Cuts at: 416 416
Size: 4568
BciVI GTATCCnnnnn_n'
Cuts at: 2466 3522 4568 2466
Size: 1056 1046 2466
Fragments arranged by size:
2466 1056 1046
BclI T'GATC_A
Cuts at: 1199 2088 1199
Size: 889 3679
BglI GCCn_nnn'nGGC
Cuts at: 1522 1522
Size: 4568
BglII A'GATC_T
Cuts at: 2083 4120 2083
Size: 2037 2531
BlpI GC'TnA_GC
Cuts at: 1680 1680
Size: 4568
Bme1580I G_kGCm'C
Cuts at: 712 1034 1055 2193 2286 3410 712
Size: 322 21 1138 93 1124 1870
Fragments arranged by size:
1870 1138 1124 322 93 21
BmgBI CAC'GTC
Cuts at: 616 616
Size: 4568
BmrI ACTGGGnnnn_n'
Cuts at: 1780 1861 2196 4340 1780
Size: 81 335 2144 2008
Fragments arranged by size:
2144 2008 335 81
BmtI G_CTAG'C
Cuts at: 884 884
Size: 4568
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1026 2935 1026
Size: 1909 2659
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 356 550 685 1010 356
Size: 194 135 325 3914
Fragments arranged by size:
3914 325 194 135
Bpu10I CC'TnA_GC
Cuts at: 487 487
Size: 4568
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 173 745 1744 2623 3337 3635 173
Size: 572 999 879 714 298 1106
Fragments arranged by size:
1106 999 879 714 572 298
BsaI GGTCTCn'nnnn_
Cuts at: 526 4272 526
Size: 3746 822
BsaAI yAC'GTr
Cuts at: 1035 1921 1035
Size: 886 3682
BsaBI GATnn'nnATC
Cuts at: 6 2082 4119 6
Size: 2076 2037 455
Fragments arranged by size:
2076 2037 455
BsaHI Gr'CG_yC
Cuts at: 112 1999 2247 2949 4445 112
Size: 1887 248 702 1496 235
Fragments arranged by size:
1887 1496 702 248 235
BsaWI w'CCGG_w
Cuts at: 1757 2278 2956 3367 3514 3788 1757
Size: 521 678 411 147 274 2537
Fragments arranged by size:
2537 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 422 452 422
Size: 30 4538
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 172 748 751 1002 1309 172
Size: 576 3 251 307 3431
Fragments arranged by size:
3431 576 307 251 3
BseYI C'CCAG_C
Cuts at: 704 790 1131 1170 1993 2751 3416 3824 704
Size: 86 341 39 823 758 665 408 1448
Fragments arranged by size:
1448 823 758 665 408 341 86 39
BsiEI CG_ry'CG
Cuts at: 104 2110 2156 3386 104
Size: 2006 46 1230 1286
Fragments arranged by size:
2006 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1034 1582 2360 2550 3410 1034
Size: 548 778 190 860 2192
Fragments arranged by size:
2192 860 778 548 190
Bsp1286I G_dGCh'C
Cuts at: 712 1034 1055 1582 2193 2286 2360 2550 2612
Size: 322 21 527 611 93 74 190 62
Cuts at: 2612 3410 712
Size: 798 1870
Fragments arranged by size:
1870 798 611 527 322 190 93 74
62 21
BspHI T'CATG_A
Cuts at: 3000 3000
Size: 4568
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1618 1832 2134 2515 1618
Size: 214 302 381 3671
Fragments arranged by size:
3671 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2860 2914 4398 4563 50
Size: 2810 54 1484 165 55
Fragments arranged by size:
2810 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 472 777 953 2480 472
Size: 305 176 1527 2560
Fragments arranged by size:
2560 1527 305 176
BsrFI r'CCGG_y
Cuts at: 1444 2566 2747 1444
Size: 1122 181 3265
Fragments arranged by size:
3265 1122 181
BsrGI T'GTAC_A
Cuts at: 523 523
Size: 4568
BssSI C'ACGA_G
Cuts at: 2839 3547 2839
Size: 708 3860
BstAPI GCAn_nnn'nTGC
Cuts at: 1945 1945
Size: 4568
BstBI TT'CG_AA
Cuts at: 2929 2929
Size: 4568
BstYI r'GATC_y
Cuts at: 1 951 1593 2060 2083 2418 2664 2970 2982
Size: 950 642 467 23 335 246 306 12
Cuts at: 2982 3068 4120 1
Size: 86 1052 449
Fragments arranged by size:
1052 950 642 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4568
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2518 2699 2862 2518
Size: 181 163 4224
Fragments arranged by size:
4224 181 163
BtsI GCAGTG_nn'
Cuts at: 637 755 1154 1532 1811 637
Size: 118 399 378 279 3394
Fragments arranged by size:
3394 399 378 279 118
DraI TTT'AAA
Cuts at: 1567 1567
Size: 4568
DrdI GACnn_nn'nnGTC
Cuts at: 812 2274 3618 812
Size: 1462 1344 1762
Fragments arranged by size:
1762 1462 1344
EaeI y'GGCC_r
Cuts at: 1529 2153 2327 2718 2745 4090 1529
Size: 624 174 391 27 1345 2007
Fragments arranged by size:
2007 1345 624 391 174 27
EagI C'GGCC_G
Cuts at: 2153 2153
Size: 4568
EarI CTCTTCn'nnn_
Cuts at: 123 296 991 1040 1061 1250 1355 1370 2591
Size: 173 695 49 21 189 105 15 1221
Cuts at: 2591 2801 123
Size: 210 1890
Fragments arranged by size:
1890 1221 695 210 189 173 105 49
21 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2776 3518 3664 4378 2776
Size: 742 146 714 2966
Fragments arranged by size:
2966 742 714 146
EcoICRI GAG'CTC
Cuts at: 1580 1580
Size: 4568
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 356 550 685 977 1010 1145 1307 2393
Size: 3 194 135 292 33 135 162 1086
Cuts at: 2393 2825 3178 3840 353
Size: 432 353 662 1081
Fragments arranged by size:
1086 1081 662 432 353 292 194 162
135 135 33 3
EcoO109I rG'GnC_Cy
Cuts at: 922 1707 922
Size: 785 3783
EcoRI G'AATT_C
Cuts at: 1572 1572
Size: 4568
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1135 1384 2587 1135
Size: 249 1203 3116
Fragments arranged by size:
3116 1203 249
FspI TGC'GCA
Cuts at: 2349 2349
Size: 4568
HaeII r_GCGC'y
Cuts at: 2002 2250 3480 4251 2002
Size: 248 1230 771 2319
Fragments arranged by size:
2319 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 295 327 1411 1443 1465 1497 2410 2440 2442
Size: 32 1084 32 22 32 913 30 2
Cuts at: 2442 2472 295
Size: 30 2391
Fragments arranged by size:
2391 1084 913 32 32 32 30 30
22 2
HincII GTy'rAC
Cuts at: 1607 1607
Size: 4568
HindIII A'AGCT_T
Cuts at: 969 969
Size: 4568
KasI G'GCGC_C
Cuts at: 1998 2246 1998
Size: 248 4320
KpnI G_GTAC'C
Cuts at: 1588 1588
Size: 4568
MluI A'CGCG_T
Cuts at: 4001 4001
Size: 4568
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 853 3327 3511 853
Size: 2474 184 1910
Fragments arranged by size:
2474 1910 184
MscI TGG'CCA
Cuts at: 1531 2329 1531
Size: 798 3770
MslI CAynn'nnrTG
Cuts at: 1195 2557 2684 1195
Size: 1362 127 3079
Fragments arranged by size:
3079 1362 127
MspA1I CmG'CkG
Cuts at: 551 1467 1679 1993 2353 3137 3382 551
Size: 916 212 314 360 784 245 1737
Fragments arranged by size:
1737 916 784 360 314 245 212
NaeI GCC'GGC
Cuts at: 2749 2749
Size: 4568
NarI GG'CG_CC
Cuts at: 1999 2247 1999
Size: 248 4320
NcoI C'CATG_G
Cuts at: 107 107
Size: 4568
NgoMIV G'CCGG_C
Cuts at: 2747 2747
Size: 4568
NheI G'CTAG_C
Cuts at: 880 880
Size: 4568
NspI r_CATG'y
Cuts at: 1621 2121 3724 1621
Size: 500 1603 2465
Fragments arranged by size:
2465 1603 500
PciI A'CATG_T
Cuts at: 3720 3720
Size: 4568
PflMI CCAn_nnn'nTGG
Cuts at: 554 845 554
Size: 291 4277
PmeI GTTT'AAAC
Cuts at: 1567 1567
Size: 4568
PmlI CAC'GTG
Cuts at: 1035 1035
Size: 4568
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 997 1029 3014 3046 997
Size: 32 1985 32 2519
Fragments arranged by size:
2519 1985 32 32
PstI C_TGCA'G
Cuts at: 647 762 1615 2300 647
Size: 115 853 685 2915
Fragments arranged by size:
2915 853 685 115
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4568
PvuII CAG'CTG
Cuts at: 1993 2353 1993
Size: 360 4208
RsrII CG'GwC_CG
Cuts at: 2763 2763
Size: 4568
SacI G_AGCT'C
Cuts at: 1582 1582
Size: 4568
SalI G'TCGA_C
Cuts at: 1605 1605
Size: 4568
SapI GCTCTTCn'nnn_
Cuts at: 296 1370 2591 2801 296
Size: 1074 1221 210 2063
Fragments arranged by size:
2063 1221 1074 210
SbfI CC_TGCA'GG
Cuts at: 1615 1615
Size: 4568
ScaI AGT'ACT
Cuts at: 4085 4085
Size: 4568
SfcI C'TryA_G
Cuts at: 33 643 758 1611 2296 3264 3455 33
Size: 610 115 853 685 968 191 1146
Fragments arranged by size:
1146 968 853 685 610 191 115
SfoI GGC'GCC
Cuts at: 2000 2248 2000
Size: 248 4320
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4568
SgrAI Cr'CCGG_yG
Cuts at: 1444 1444
Size: 4568
SmaI CCC'GGG
Cuts at: 1590 1590
Size: 4568
SmlI C'TyrA_G
Cuts at: 188 724 1723 2638 3352 3614 188
Size: 536 999 915 714 262 1142
Fragments arranged by size:
1142 999 915 714 536 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4568
SphI G_CATG'C
Cuts at: 1621 2121 1621
Size: 500 4068
StyI C'CwwG_G
Cuts at: 107 225 1702 2056 107
Size: 118 1477 354 2619
Fragments arranged by size:
2619 1477 354 118
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2151 2284 2743 2952 2151
Size: 133 459 209 3767
Fragments arranged by size:
3767 459 209 133
TatI w'GTAC_w
Cuts at: 523 683 4083 523
Size: 160 3400 1008
Fragments arranged by size:
3400 1008 160
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 422 454 4085 4117 422
Size: 32 3631 32 873
Fragments arranged by size:
3631 873 32 32
Tth111I GACn'n_nGTC
Cuts at: 617 2365 617
Size: 1748 2820
XbaI T'CTAG_A
Cuts at: 1429 1599 1429
Size: 170 4398
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1167 1524 2063 1167
Size: 357 539 3672
Fragments arranged by size:
3672 539 357
XmaI C'CCGG_G
Cuts at: 1588 1588
Size: 4568
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PciI PflMI PmeI PmlI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BsgI BsiWI
BsmI BsmBI BspEI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI
MfeI NdeI NotI NruI NsiI PacI PasI PfoI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI