Restriction map for
pF1KB8457
(Circular) (Six-base) MAPSORT of: pF1KB8457.seq Check: 2227 from: 1 to: 4286
pF1KB8457 4286 bp
With 182 enzymes: SgfI *
June 20, 2008 10:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 900 900
Size: 4286
AccI GT'mk_AC
Cuts at: 1324 1324
Size: 4286
Acc65I G'GTAC_C
Cuts at: 1302 1302
Size: 4286
AclI AA'CG_TT
Cuts at: 4126 4126
Size: 4286
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2111 2543 2896 3558 2111
Size: 432 353 662 2839
Fragments arranged by size:
2839 662 432 353
AflIII A'CryG_T
Cuts at: 287 1638 3438 3719 287
Size: 1351 1800 281 854
Fragments arranged by size:
1800 1351 854 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 451 483 568 600 451
Size: 32 85 32 4137
Fragments arranged by size:
4137 85 32 32
AlwNI CAG_nnn'CTG
Cuts at: 871 892 934 1000 1067 1591 3029 871
Size: 21 42 66 67 524 1438 2128
Fragments arranged by size:
2128 1438 524 67 66 42 21
ApaI G_GGCC'C
Cuts at: 719 994 719
Size: 275 4011
ApaLI G'TGCA_C
Cuts at: 3124 3124
Size: 4286
ApoI r'AATT_y
Cuts at: 1243 1290 3639 3895 1243
Size: 47 2349 256 1634
Fragments arranged by size:
2349 1634 256 47
AseI AT'TA_AT
Cuts at: 20 20
Size: 4286
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4286
AvaI C'yCGr_G
Cuts at: 232 661 1034 1148 1306 232
Size: 429 373 114 158 3212
Fragments arranged by size:
3212 429 373 158 114
BamHI G'GATC_C
Cuts at: 1311 1311
Size: 4286
BanI G'GyrC_C
Cuts at: 133 387 1185 1302 1716 1964 1999 133
Size: 254 798 117 414 248 35 2420
Fragments arranged by size:
2420 798 414 254 248 117 35
BanII G_rGCy'C
Cuts at: 662 719 994 1300 2330 662
Size: 57 275 306 1030 2618
Fragments arranged by size:
2618 1030 306 275 57
BbeI G_GCGC'C
Cuts at: 137 391 1189 1720 1968 137
Size: 254 798 531 248 2455
Fragments arranged by size:
2455 798 531 254 248
BbsI GAAGACnn'nnnn_
Cuts at: 270 270
Size: 4286
BbvCI CC'TCA_GC
Cuts at: 298 727 885 1001 1256 298
Size: 429 158 116 255 3328
Fragments arranged by size:
3328 429 255 158 116
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4252
BciVI GTATCCnnnnn_n'
Cuts at: 2184 3240 4286 2184
Size: 1056 1046 2184
Fragments arranged by size:
2184 1056 1046
BclI T'GATC_A
Cuts at: 218 1806 218
Size: 1588 2698
BfrBI ATG'CAT
Cuts at: 255 255
Size: 4286
BglII A'GATC_T
Cuts at: 1801 3838 1801
Size: 2037 2249
BlpI GC'TnA_GC
Cuts at: 520 1398 520
Size: 878 3408
Bme1580I G_kGCm'C
Cuts at: 719 994 1217 1911 2004 3128 719
Size: 275 223 694 93 1124 1877
Fragments arranged by size:
1877 1124 694 275 223 93
BmgBI CAC'GTC
Cuts at: 290 290
Size: 4286
BmrI ACTGGGnnnn_n'
Cuts at: 988 1012 1077 1204 1498 1579 1914 4058 988
Size: 24 65 127 294 81 335 2144 1216
Fragments arranged by size:
2144 1216 335 294 127 81 65 24
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2653 2653
Size: 4286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 546 764 546
Size: 218 4068
Bpu10I CC'TnA_GC
Cuts at: 298 727 885 1001 1256 298
Size: 429 158 116 255 3328
Fragments arranged by size:
3328 429 255 158 116
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1462 2341 3055 3353 1462
Size: 879 714 298 2395
Fragments arranged by size:
2395 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 626 3990 626
Size: 3364 922
BsaAI yAC'GTr
Cuts at: 1639 1639
Size: 4286
BsaBI GATnn'nnATC
Cuts at: 6 1800 3837 6
Size: 1794 2037 455
Fragments arranged by size:
2037 1794 455
BsaHI Gr'CG_yC
Cuts at: 134 388 1186 1717 1965 2667 4163 134
Size: 254 798 531 248 702 1496 257
Fragments arranged by size:
1496 798 702 531 257 254 248
BsaWI w'CCGG_w
Cuts at: 1475 1996 2674 3085 3232 3506 1475
Size: 521 678 411 147 274 2255
Fragments arranged by size:
2255 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 451 481 544 574 451
Size: 30 63 30 4163
Fragments arranged by size:
4163 63 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 715 1273 715
Size: 558 3728
BseYI C'CCAG_C
Cuts at: 173 476 801 843 864 927 982 1079 1216
Size: 303 325 42 21 63 55 97 137
Cuts at: 1216 1711 2469 3134 3542 173
Size: 495 758 665 408 917
Fragments arranged by size:
917 758 665 495 408 325 303 137
97 63 55 42 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 401 573 401
Size: 172 4114
BsiEI CG_ry'CG
Cuts at: 104 1828 1874 3104 104
Size: 1724 46 1230 1286
Fragments arranged by size:
1724 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 328 1300 2078 2268 3128 328
Size: 972 778 190 860 1486
Fragments arranged by size:
1486 972 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 328 662 719 994 1217 1300 1911 2004 2078
Size: 334 57 275 223 83 611 93 74
Cuts at: 2078 2268 2330 3128 328
Size: 190 62 798 1486
Fragments arranged by size:
1486 798 611 334 275 223 190 93
83 74 62 57
BspHI T'CATG_A
Cuts at: 746 2718 746
Size: 1972 2314
BspMI ACCTGCnnnn'nnnn_
Cuts at: 900 1050 1336 1550 1852 2233 900
Size: 150 286 214 302 381 2953
Fragments arranged by size:
2953 381 302 286 214 150
BsrBI CCG'CTC
Cuts at: 50 611 674 1125 2578 2632 4116 4281 50
Size: 561 63 451 1453 54 1484 165 55
Fragments arranged by size:
1484 1453 561 451 165 63 55 54
BsrDI GCAATG_nn'
Cuts at: 2198 2198
Size: 4286
BsrFI r'CCGG_y
Cuts at: 435 2284 2465 435
Size: 1849 181 2256
Fragments arranged by size:
2256 1849 181
BssSI C'ACGA_G
Cuts at: 505 2557 3265 505
Size: 2052 708 1526
Fragments arranged by size:
2052 1526 708
BstAPI GCAn_nnn'nTGC
Cuts at: 372 1663 372
Size: 1291 2995
BstBI TT'CG_AA
Cuts at: 2647 2647
Size: 4286
BstXI CCAn_nnnn'nTGG
Cuts at: 150 258 1020 150
Size: 108 762 3416
Fragments arranged by size:
3416 762 108
BstYI r'GATC_y
Cuts at: 1 1311 1778 1801 2136 2382 2688 2700 2786
Size: 1310 467 23 335 246 306 12 86
Cuts at: 2786 3838 1
Size: 1052 449
Fragments arranged by size:
1310 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 264 306 548 821 905 954 264
Size: 42 242 273 84 49 3596
Fragments arranged by size:
3596 273 242 84 49 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 316 2236 2417 2580 316
Size: 1920 181 163 2022
Fragments arranged by size:
2022 1920 181 163
BtsI GCAGTG_nn'
Cuts at: 1529 1529
Size: 4286
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 291 326 291
Size: 35 4251
DraI TTT'AAA
Cuts at: 1285 1285
Size: 4286
DrdI GACnn_nn'nnGTC
Cuts at: 1992 3336 1992
Size: 1344 2942
EaeI y'GGCC_r
Cuts at: 353 437 555 1089 1871 2045 2436 2463 3808
Size: 84 118 534 782 174 391 27 1345
Cuts at: 3808 353
Size: 831
Fragments arranged by size:
1345 831 782 534 391 174 118 84
27
EagI C'GGCC_G
Cuts at: 1871 1871
Size: 4286
EarI CTCTTCn'nnn_
Cuts at: 1169 2309 2519 1169
Size: 1140 210 2936
Fragments arranged by size:
2936 1140 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 232 685 2494 3236 3382 4096 232
Size: 453 1809 742 146 714 422
Fragments arranged by size:
1809 742 714 453 422 146
EcoICRI GAG'CTC
Cuts at: 1298 1298
Size: 4286
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 546 764 2111 2543 2896 3558 546
Size: 218 1347 432 353 662 1274
Fragments arranged by size:
1347 1274 662 432 353 218
EcoO109I rG'GnC_Cy
Cuts at: 716 826 910 1030 1039 1205 1425 716
Size: 110 84 120 9 166 220 3577
Fragments arranged by size:
3577 220 166 120 110 84 9
EcoRI G'AATT_C
Cuts at: 1290 1290
Size: 4286
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 540 2305 540
Size: 1765 2521
FspI TGC'GCA
Cuts at: 2067 2067
Size: 4286
HaeII r_GCGC'y
Cuts at: 137 391 871 1189 1720 1968 3198 3969 137
Size: 254 480 318 531 248 1230 771 454
Fragments arranged by size:
1230 771 531 480 454 318 254 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 315 347 393 425 544 576 994 1026 2128
Size: 32 46 32 119 32 418 32 1102
Cuts at: 2128 2158 2160 2190 315
Size: 30 2 30 2411
Fragments arranged by size:
2411 1102 418 119 46 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1325 1325
Size: 4286
KasI G'GCGC_C
Cuts at: 133 387 1185 1716 1964 133
Size: 254 798 531 248 2455
Fragments arranged by size:
2455 798 531 254 248
KpnI G_GTAC'C
Cuts at: 1306 1306
Size: 4286
MluI A'CGCG_T
Cuts at: 3719 3719
Size: 4286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3045 3229 3045
Size: 184 4102
MscI TGG'CCA
Cuts at: 355 2047 355
Size: 1692 2594
MslI CAynn'nnrTG
Cuts at: 256 2275 2402 256
Size: 2019 127 2140
Fragments arranged by size:
2140 2019 127
MspA1I CmG'CkG
Cuts at: 369 772 790 982 1397 1711 2071 2855 3100
Size: 403 18 192 415 314 360 784 245
Cuts at: 3100 369
Size: 1555
Fragments arranged by size:
1555 784 415 403 360 314 245 192
18
NaeI GCC'GGC
Cuts at: 2467 2467
Size: 4286
NarI GG'CG_CC
Cuts at: 134 388 1186 1717 1965 134
Size: 254 798 531 248 2455
Fragments arranged by size:
2455 798 531 254 248
NcoI C'CATG_G
Cuts at: 954 954
Size: 4286
NgoMIV G'CCGG_C
Cuts at: 2465 2465
Size: 4286
NsiI A_TGCA'T
Cuts at: 257 257
Size: 4286
NspI r_CATG'y
Cuts at: 259 1339 1839 3442 259
Size: 1080 500 1603 1103
Fragments arranged by size:
1603 1103 1080 500
PasI CC'CwG_GG
Cuts at: 120 120
Size: 4286
PciI A'CATG_T
Cuts at: 3438 3438
Size: 4286
PfoI T'CCnGG_A
Cuts at: 319 319
Size: 4286
PmeI GTTT'AAAC
Cuts at: 1285 1285
Size: 4286
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 451 483 2732 2764 451
Size: 32 2249 32 1973
Fragments arranged by size:
2249 1973 32 32
PpuMI rG'GwC_Cy
Cuts at: 826 910 1030 1039 1205 826
Size: 84 120 9 166 3907
Fragments arranged by size:
3907 166 120 84 9
PspOMI G'GGCC_C
Cuts at: 715 990 715
Size: 275 4011
PstI C_TGCA'G
Cuts at: 213 368 771 1333 2018 213
Size: 155 403 562 685 2481
Fragments arranged by size:
2481 685 562 403 155
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4286
PvuII CAG'CTG
Cuts at: 369 772 982 1711 2071 369
Size: 403 210 729 360 2584
Fragments arranged by size:
2584 729 403 360 210
RsrII CG'GwC_CG
Cuts at: 2481 2481
Size: 4286
SacI G_AGCT'C
Cuts at: 1300 1300
Size: 4286
SalI G'TCGA_C
Cuts at: 1323 1323
Size: 4286
SanDI GG'GwC_CC
Cuts at: 1030 1030
Size: 4286
SapI GCTCTTCn'nnn_
Cuts at: 2309 2519 2309
Size: 210 4076
SbfI CC_TGCA'GG
Cuts at: 1333 1333
Size: 4286
ScaI AGT'ACT
Cuts at: 3803 3803
Size: 4286
SfcI C'TryA_G
Cuts at: 33 209 364 767 1329 2014 2982 3173 33
Size: 176 155 403 562 685 968 191 1146
Fragments arranged by size:
1146 968 685 562 403 191 176 155
SfoI GGC'GCC
Cuts at: 135 389 1187 1718 1966 135
Size: 254 798 531 248 2455
Fragments arranged by size:
2455 798 531 254 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4286
SmaI CCC'GGG
Cuts at: 663 1150 1308 663
Size: 487 158 3641
Fragments arranged by size:
3641 487 158
SmlI C'TyrA_G
Cuts at: 1441 2356 3070 3332 1441
Size: 915 714 262 2395
Fragments arranged by size:
2395 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4286
SphI G_CATG'C
Cuts at: 1339 1839 1339
Size: 500 3786
SrfI GCCC'GGGC
Cuts at: 663 1150 663
Size: 487 3799
StyI C'CwwG_G
Cuts at: 711 954 1420 1774 711
Size: 243 466 354 3223
Fragments arranged by size:
3223 466 354 243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 165 242 343 644 1869 2002 2461 2670 165
Size: 77 101 301 1225 133 459 209 1781
Fragments arranged by size:
1781 1225 459 301 209 133 101 77
TatI w'GTAC_w
Cuts at: 3801 3801
Size: 4286
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1162 1194 3803 3835 1162
Size: 32 2609 32 1613
Fragments arranged by size:
2609 1613 32 32
Tth111I GACn'n_nGTC
Cuts at: 962 2083 962
Size: 1121 3165
XbaI T'CTAG_A
Cuts at: 1317 1317
Size: 4286
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 147 570 1781 147
Size: 423 1211 2652
Fragments arranged by size:
2652 1211 423
XmaI C'CCGG_G
Cuts at: 661 1148 1306 661
Size: 487 158 3641
Fragments arranged by size:
3641 487 158
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AlfI AscI
AvrII BaeI BglI BmtI BsiWI BsmI BsmBI BspEI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PflMI PmlI PshAI PsiI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SspI StuI
SwaI XhoI XmnI ZraI