Restriction map for
pF1KB8460
(Circular) (Six-base) MAPSORT of: pF1KB8460.seq Check: 1438 from: 1 to: 3869
pF1KB8460 3869 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 907 907
Size: 3869
Acc65I G'GTAC_C
Cuts at: 885 885
Size: 3869
AclI AA'CG_TT
Cuts at: 3709 3709
Size: 3869
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 717 1694 2126 2479 3141 717
Size: 977 432 353 662 1445
Fragments arranged by size:
1445 977 662 432 353
AflIII A'CryG_T
Cuts at: 1221 3021 3302 1221
Size: 1800 281 1788
Fragments arranged by size:
1800 1788 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 706 706
Size: 3869
AlwNI CAG_nnn'CTG
Cuts at: 576 789 1174 2612 576
Size: 213 385 1438 1833
Fragments arranged by size:
1833 1438 385 213
ApaLI G'TGCA_C
Cuts at: 2707 2707
Size: 3869
ApoI r'AATT_y
Cuts at: 873 3222 3478 873
Size: 2349 256 1264
Fragments arranged by size:
2349 1264 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3869
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3869
AvaI C'yCGr_G
Cuts at: 340 538 860 889 340
Size: 198 322 29 3320
Fragments arranged by size:
3320 322 198 29
BamHI G'GATC_C
Cuts at: 894 894
Size: 3869
BanI G'GyrC_C
Cuts at: 885 1299 1547 1582 885
Size: 414 248 35 3172
Fragments arranged by size:
3172 414 248 35
BanII G_rGCy'C
Cuts at: 545 602 754 883 1913 545
Size: 57 152 129 1030 2501
Fragments arranged by size:
2501 1030 152 129 57
BbeI G_GCGC'C
Cuts at: 1303 1551 1303
Size: 248 3621
BbsI GAAGACnn'nnnn_
Cuts at: 723 723
Size: 3869
BciVI GTATCCnnnnn_n'
Cuts at: 301 1767 2823 3869 301
Size: 1466 1056 1046 301
Fragments arranged by size:
1466 1056 1046 301
BclI T'GATC_A
Cuts at: 1389 1389
Size: 3869
BglII A'GATC_T
Cuts at: 246 1384 3421 246
Size: 1138 2037 694
Fragments arranged by size:
2037 1138 694
BlpI GC'TnA_GC
Cuts at: 596 981 596
Size: 385 3484
Bme1580I G_kGCm'C
Cuts at: 1494 1587 2711 1494
Size: 93 1124 2652
Fragments arranged by size:
2652 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 347 424 1081 1162 1497 3641 347
Size: 77 657 81 335 2144 575
Fragments arranged by size:
2144 657 575 335 81 77
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2236 2236
Size: 3869
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 694 804 217
Size: 477 110 3282
Fragments arranged by size:
3282 477 110
Bpu10I CC'TnA_GC
Cuts at: 544 544
Size: 3869
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1045 1924 2638 2936 1045
Size: 879 714 298 1978
Fragments arranged by size:
1978 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 355 3573 355
Size: 3218 651
BsaAI yAC'GTr
Cuts at: 289 1222 289
Size: 933 2936
BsaBI GATnn'nnATC
Cuts at: 6 1383 3420 6
Size: 1377 2037 455
Fragments arranged by size:
2037 1377 455
BsaHI Gr'CG_yC
Cuts at: 1300 1548 2250 3746 1300
Size: 248 702 1496 1423
Fragments arranged by size:
1496 1423 702 248
BsaWI w'CCGG_w
Cuts at: 758 1058 1579 2257 2668 2815 3089 758
Size: 300 521 678 411 147 274 1538
Fragments arranged by size:
1538 678 521 411 300 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 373 373
Size: 3869
BseYI C'CCAG_C
Cuts at: 375 569 1294 2052 2717 3125 375
Size: 194 725 758 665 408 1119
Fragments arranged by size:
1119 758 725 665 408 194
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 412
Size: 3869
BsiEI CG_ry'CG
Cuts at: 104 1411 1457 2687 104
Size: 1307 46 1230 1286
Fragments arranged by size:
1307 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 221 326 883 1661 1851 2711 221
Size: 105 557 778 190 860 1379
Fragments arranged by size:
1379 860 778 557 190 105
Bsp1286I G_dGCh'C
Cuts at: 221 326 545 602 754 883 1494 1587 1661
Size: 105 219 57 152 129 611 93 74
Cuts at: 1661 1851 1913 2711 221
Size: 190 62 798 1379
Fragments arranged by size:
1379 798 611 219 190 152 129 105
93 74 62 57
BspHI T'CATG_A
Cuts at: 839 2301 839
Size: 1462 2407
BspMI ACCTGCnnnn'nnnn_
Cuts at: 674 919 1133 1435 1816 674
Size: 245 214 302 381 2727
Fragments arranged by size:
2727 381 302 245 214
BsrBI CCG'CTC
Cuts at: 50 2161 2215 3699 3864 50
Size: 2111 54 1484 165 55
Fragments arranged by size:
2111 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1781 1781
Size: 3869
BsrFI r'CCGG_y
Cuts at: 1867 2048 1867
Size: 181 3688
BssSI C'ACGA_G
Cuts at: 2140 2848 2140
Size: 708 3161
BstAPI GCAn_nnn'nTGC
Cuts at: 1246 1246
Size: 3869
BstBI TT'CG_AA
Cuts at: 2230 2230
Size: 3869
BstYI r'GATC_y
Cuts at: 1 246 894 1361 1384 1719 1965 2271 2283
Size: 245 648 467 23 335 246 306 12
Cuts at: 2283 2369 3421 1
Size: 86 1052 449
Fragments arranged by size:
1052 648 467 449 335 306 246 245
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 367 790 367
Size: 423 3446
BtgI C'CryG_G
Cuts at: 107 107
Size: 3869
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 688 1819 2000 2163 688
Size: 1131 181 163 2394
Fragments arranged by size:
2394 1131 181 163
BtsI GCAGTG_nn'
Cuts at: 324 1112 324
Size: 788 3081
DraI TTT'AAA
Cuts at: 868 868
Size: 3869
DraIII CAC_nnn'GTG
Cuts at: 720 720
Size: 3869
DrdI GACnn_nn'nnGTC
Cuts at: 1575 2919 1575
Size: 1344 2525
EaeI y'GGCC_r
Cuts at: 225 437 612 1454 1628 2019 2046 3391 225
Size: 212 175 842 174 391 27 1345 703
Fragments arranged by size:
1345 842 703 391 212 175 174 27
EagI C'GGCC_G
Cuts at: 1454 1454
Size: 3869
EarI CTCTTCn'nnn_
Cuts at: 1892 2102 1892
Size: 210 3659
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2077 2819 2965 3679 2077
Size: 742 146 714 2267
Fragments arranged by size:
2267 742 714 146
EcoICRI GAG'CTC
Cuts at: 881 881
Size: 3869
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 694 717 804 1694 2126 2479 3141 217
Size: 477 23 87 890 432 353 662 945
Fragments arranged by size:
945 890 662 477 432 353 87 23
EcoNI CCTnn'n_nnAGG
Cuts at: 460 793 460
Size: 333 3536
EcoO109I rG'GnC_Cy
Cuts at: 344 620 786 1008 344
Size: 276 166 222 3205
Fragments arranged by size:
3205 276 222 166
EcoRI G'AATT_C
Cuts at: 873 873
Size: 3869
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1888 1888
Size: 3869
FspI TGC'GCA
Cuts at: 805 1650 805
Size: 845 3024
HaeII r_GCGC'y
Cuts at: 339 456 1303 1551 2781 3552 339
Size: 117 847 248 1230 771 656
Fragments arranged by size:
1230 847 771 656 248 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1711 1741 1743 1773 1711
Size: 30 2 30 3807
Fragments arranged by size:
3807 30 30 2
HincII GTy'rAC
Cuts at: 908 908
Size: 3869
KasI G'GCGC_C
Cuts at: 1299 1547 1299
Size: 248 3621
KpnI G_GTAC'C
Cuts at: 889 889
Size: 3869
MluI A'CGCG_T
Cuts at: 3302 3302
Size: 3869
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2628 2812 2628
Size: 184 3685
MscI TGG'CCA
Cuts at: 227 1630 227
Size: 1403 2466
MslI CAynn'nnrTG
Cuts at: 1858 1985 1858
Size: 127 3742
MspA1I CmG'CkG
Cuts at: 197 573 855 980 1294 1654 2438 2683 197
Size: 376 282 125 314 360 784 245 1383
Fragments arranged by size:
1383 784 376 360 314 282 245 125
NaeI GCC'GGC
Cuts at: 2050 2050
Size: 3869
NarI GG'CG_CC
Cuts at: 1300 1548 1300
Size: 248 3621
NcoI C'CATG_G
Cuts at: 107 107
Size: 3869
NgoMIV G'CCGG_C
Cuts at: 2048 2048
Size: 3869
NspI r_CATG'y
Cuts at: 922 1422 3025 922
Size: 500 1603 1766
Fragments arranged by size:
1766 1603 500
PciI A'CATG_T
Cuts at: 3021 3021
Size: 3869
PflMI CCAn_nnn'nTGG
Cuts at: 300 474 576 300
Size: 174 102 3593
Fragments arranged by size:
3593 174 102
PfoI T'CCnGG_A
Cuts at: 272 820 272
Size: 548 3321
PmeI GTTT'AAAC
Cuts at: 868 868
Size: 3869
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2315 2347 2315
Size: 32 3837
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 541 573 541
Size: 32 3837
PstI C_TGCA'G
Cuts at: 331 398 518 685 916 1601 331
Size: 67 120 167 231 685 2599
Fragments arranged by size:
2599 685 231 167 120 67
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3869
PvuII CAG'CTG
Cuts at: 197 573 855 1294 1654 197
Size: 376 282 439 360 2412
Fragments arranged by size:
2412 439 376 360 282
RsrII CG'GwC_CG
Cuts at: 2064 2064
Size: 3869
SacI G_AGCT'C
Cuts at: 883 883
Size: 3869
SalI G'TCGA_C
Cuts at: 906 906
Size: 3869
SapI GCTCTTCn'nnn_
Cuts at: 1892 2102 1892
Size: 210 3659
SbfI CC_TGCA'GG
Cuts at: 916 916
Size: 3869
ScaI AGT'ACT
Cuts at: 3386 3386
Size: 3869
SfcI C'TryA_G
Cuts at: 33 188 327 394 514 681 912 1597 2565
Size: 155 139 67 120 167 231 685 968
Cuts at: 2565 2756 33
Size: 191 1146
Fragments arranged by size:
1146 968 685 231 191 167 155 139
120 67
SfoI GGC'GCC
Cuts at: 1301 1549 1301
Size: 248 3621
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3869
SmaI CCC'GGG
Cuts at: 342 862 891 342
Size: 520 29 3320
Fragments arranged by size:
3320 520 29
SmlI C'TyrA_G
Cuts at: 1024 1939 2653 2915 1024
Size: 915 714 262 1978
Fragments arranged by size:
1978 915 714 262
SnaBI TAC'GTA
Cuts at: 289 289
Size: 3869
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3869
SphI G_CATG'C
Cuts at: 922 1422 922
Size: 500 3369
StuI AGG'CCT
Cuts at: 299 299
Size: 3869
StyI C'CwwG_G
Cuts at: 107 222 294 461 1003 1357 107
Size: 115 72 167 542 354 2619
Fragments arranged by size:
2619 542 354 167 115 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1452 1585 2044 2253 1452
Size: 133 459 209 3068
Fragments arranged by size:
3068 459 209 133
TatI w'GTAC_w
Cuts at: 3384 3384
Size: 3869
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3386 3418 3386
Size: 32 3837
Tth111I GACn'n_nGTC
Cuts at: 1666 1666
Size: 3869
XbaI T'CTAG_A
Cuts at: 900 900
Size: 3869
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 270 1364 270
Size: 1094 2775
XmaI C'CCGG_G
Cuts at: 340 860 889 340
Size: 520 29 3320
Fragments arranged by size:
3320 520 29
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BsaXI BsiWI BsmI BsmBI BspEI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PpuMI PshAI PsiI PspOMI
PspXI SacII SanDI SexAI SfiI SgrAI SrfI SspI
SwaI XhoI XmnI ZraI