Restriction map for
pF1KB8461
(Circular) (Six-base) MAPSORT of: pF1KB8461.seq Check: 9099 from: 1 to: 3689
pF1KB8461 3689 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AatII G_ACGT'C
Cuts at: 338 338
Size: 3689
AccI GT'mk_AC
Cuts at: 727 727
Size: 3689
Acc65I G'GTAC_C
Cuts at: 705 705
Size: 3689
AclI AA'CG_TT
Cuts at: 3529 3529
Size: 3689
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 1514 1946 2299 2961 514
Size: 1000 432 353 662 1242
Fragments arranged by size:
1242 1000 662 432 353
AflIII A'CryG_T
Cuts at: 1041 2841 3122 1041
Size: 1800 281 1608
Fragments arranged by size:
1800 1608 281
AlwNI CAG_nnn'CTG
Cuts at: 534 994 2432 534
Size: 460 1438 1791
Fragments arranged by size:
1791 1438 460
ApaLI G'TGCA_C
Cuts at: 2527 2527
Size: 3689
ApoI r'AATT_y
Cuts at: 693 3042 3298 693
Size: 2349 256 1084
Fragments arranged by size:
2349 1084 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3689
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3689
AvaI C'yCGr_G
Cuts at: 709 709
Size: 3689
BamHI G'GATC_C
Cuts at: 714 714
Size: 3689
BanI G'GyrC_C
Cuts at: 413 448 705 1119 1367 1402 413
Size: 35 257 414 248 35 2700
Fragments arranged by size:
2700 414 257 248 35 35
BanII G_rGCy'C
Cuts at: 703 1733 703
Size: 1030 2659
BbeI G_GCGC'C
Cuts at: 1123 1371 1123
Size: 248 3441
BbsI GAAGACnn'nnnn_
Cuts at: 330 330
Size: 3689
BbvCI CC'TCA_GC
Cuts at: 644 644
Size: 3689
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 663 697 90
Size: 34 539 34 3082
Fragments arranged by size:
3082 539 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1587 2643 3689 1587
Size: 1056 1046 1587
Fragments arranged by size:
1587 1056 1046
BclI T'GATC_A
Cuts at: 1209 1209
Size: 3689
BglI GCCn_nnn'nGGC
Cuts at: 112 628 654 112
Size: 516 26 3147
Fragments arranged by size:
3147 516 26
BglII A'GATC_T
Cuts at: 1204 3241 1204
Size: 2037 1652
BlpI GC'TnA_GC
Cuts at: 579 801 579
Size: 222 3467
Bme1580I G_kGCm'C
Cuts at: 451 643 1314 1407 2531 451
Size: 192 671 93 1124 1609
Fragments arranged by size:
1609 1124 671 192 93
BmrI ACTGGGnnnn_n'
Cuts at: 901 982 1317 3461 901
Size: 81 335 2144 1129
Fragments arranged by size:
2144 1129 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 573 2056 573
Size: 1483 2206
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 592 592
Size: 3689
Bpu10I CC'TnA_GC
Cuts at: 644 644
Size: 3689
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 865 1744 2458 2756 865
Size: 879 714 298 1798
Fragments arranged by size:
1798 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3393 3393
Size: 3689
BsaAI yAC'GTr
Cuts at: 1042 1042
Size: 3689
BsaBI GATnn'nnATC
Cuts at: 6 1203 3240 6
Size: 1197 2037 455
Fragments arranged by size:
2037 1197 455
BsaHI Gr'CG_yC
Cuts at: 335 1120 1368 2070 3566 335
Size: 785 248 702 1496 458
Fragments arranged by size:
1496 785 702 458 248
BsaWI w'CCGG_w
Cuts at: 878 1399 2077 2488 2635 2909 878
Size: 521 678 411 147 274 1658
Fragments arranged by size:
1658 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 670 670
Size: 3689
BseYI C'CCAG_C
Cuts at: 283 366 424 1114 1872 2537 2945 283
Size: 83 58 690 758 665 408 1027
Fragments arranged by size:
1027 758 690 665 408 83 58
BsiEI CG_ry'CG
Cuts at: 104 303 313 1231 1277 2507 104
Size: 199 10 918 46 1230 1286
Fragments arranged by size:
1286 1230 918 199 46 10
BsiHKAI G_wGCw'C
Cuts at: 580 703 1481 1671 2531 580
Size: 123 778 190 860 1738
Fragments arranged by size:
1738 860 778 190 123
BsmBI CGTCTCn'nnnn_
Cuts at: 310 310
Size: 3689
Bsp1286I G_dGCh'C
Cuts at: 451 580 643 703 1314 1407 1481 1671 1733
Size: 129 63 60 611 93 74 190 62
Cuts at: 1733 2531 451
Size: 798 1609
Fragments arranged by size:
1609 798 611 190 129 93 74 63
62 60
BspHI T'CATG_A
Cuts at: 2121 2121
Size: 3689
BspMI ACCTGCnnnn'nnnn_
Cuts at: 166 470 739 953 1255 1636 166
Size: 304 269 214 302 381 2219
Fragments arranged by size:
2219 381 304 302 269 214
BsrBI CCG'CTC
Cuts at: 50 315 1981 2035 3519 3684 50
Size: 265 1666 54 1484 165 55
Fragments arranged by size:
1666 1484 265 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1601 1601
Size: 3689
BsrFI r'CCGG_y
Cuts at: 1687 1868 1687
Size: 181 3508
BssHII G'CGCG_C
Cuts at: 666 666
Size: 3689
BssSI C'ACGA_G
Cuts at: 1960 2668 1960
Size: 708 2981
BstAPI GCAn_nnn'nTGC
Cuts at: 158 1066 158
Size: 908 2781
BstBI TT'CG_AA
Cuts at: 2050 2050
Size: 3689
BstXI CCAn_nnnn'nTGG
Cuts at: 240 441 240
Size: 201 3488
BstYI r'GATC_y
Cuts at: 1 714 1181 1204 1539 1785 2091 2103 2189
Size: 713 467 23 335 246 306 12 86
Cuts at: 2189 3241 1
Size: 1052 449
Fragments arranged by size:
1052 713 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 146 146
Size: 3689
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1639 1820 1983 1639
Size: 181 163 3345
Fragments arranged by size:
3345 181 163
BtsI GCAGTG_nn'
Cuts at: 278 417 932 278
Size: 139 515 3035
Fragments arranged by size:
3035 515 139
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 198 233 198
Size: 35 3654
DraI TTT'AAA
Cuts at: 688 688
Size: 3689
DrdI GACnn_nn'nnGTC
Cuts at: 1395 2739 1395
Size: 1344 2345
EaeI y'GGCC_r
Cuts at: 310 542 1274 1448 1839 1866 3211 310
Size: 232 732 174 391 27 1345 788
Fragments arranged by size:
1345 788 732 391 232 174 27
EagI C'GGCC_G
Cuts at: 310 1274 310
Size: 964 2725
EarI CTCTTCn'nnn_
Cuts at: 1712 1922 1712
Size: 210 3479
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1897 2639 2785 3499 1897
Size: 742 146 714 2087
Fragments arranged by size:
2087 742 714 146
EcoICRI GAG'CTC
Cuts at: 701 701
Size: 3689
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 592 1514 1946 2299 2961 514
Size: 78 922 432 353 662 1242
Fragments arranged by size:
1242 922 662 432 353 78
EcoO109I rG'GnC_Cy
Cuts at: 531 828 531
Size: 297 3392
EcoRI G'AATT_C
Cuts at: 693 693
Size: 3689
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1708 1708
Size: 3689
FspI TGC'GCA
Cuts at: 1470 1470
Size: 3689
HaeII r_GCGC'y
Cuts at: 1123 1371 2601 3372 1123
Size: 248 1230 771 1440
Fragments arranged by size:
1440 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 188 220 1531 1561 1563 1593 188
Size: 32 1311 30 2 30 2284
Fragments arranged by size:
2284 1311 32 30 30 2
HincII GTy'rAC
Cuts at: 728 728
Size: 3689
KasI G'GCGC_C
Cuts at: 1119 1367 1119
Size: 248 3441
KpnI G_GTAC'C
Cuts at: 709 709
Size: 3689
MluI A'CGCG_T
Cuts at: 3122 3122
Size: 3689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2448 2632 2448
Size: 184 3505
MscI TGG'CCA
Cuts at: 544 1450 544
Size: 906 2783
MslI CAynn'nnrTG
Cuts at: 442 1678 1805 442
Size: 1236 127 2326
Fragments arranged by size:
2326 1236 127
MspA1I CmG'CkG
Cuts at: 800 1114 1474 2258 2503 800
Size: 314 360 784 245 1986
Fragments arranged by size:
1986 784 360 314 245
NaeI GCC'GGC
Cuts at: 1870 1870
Size: 3689
NarI GG'CG_CC
Cuts at: 1120 1368 1120
Size: 248 3441
NgoMIV G'CCGG_C
Cuts at: 1868 1868
Size: 3689
NspI r_CATG'y
Cuts at: 742 1242 2845 742
Size: 500 1603 1586
Fragments arranged by size:
1603 1586 500
PciI A'CATG_T
Cuts at: 2841 2841
Size: 3689
PfoI T'CCnGG_A
Cuts at: 192 192
Size: 3689
PmeI GTTT'AAAC
Cuts at: 688 688
Size: 3689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2135 2167 2135
Size: 32 3657
PshAI GACnn'nnGTC
Cuts at: 334 334
Size: 3689
PstI C_TGCA'G
Cuts at: 676 736 1421 676
Size: 60 685 2944
Fragments arranged by size:
2944 685 60
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3689
PvuII CAG'CTG
Cuts at: 1114 1474 1114
Size: 360 3329
RsrII CG'GwC_CG
Cuts at: 1884 1884
Size: 3689
SacI G_AGCT'C
Cuts at: 703 703
Size: 3689
SalI G'TCGA_C
Cuts at: 726 726
Size: 3689
SapI GCTCTTCn'nnn_
Cuts at: 1712 1922 1712
Size: 210 3479
SbfI CC_TGCA'GG
Cuts at: 736 736
Size: 3689
ScaI AGT'ACT
Cuts at: 3206 3206
Size: 3689
SfcI C'TryA_G
Cuts at: 33 672 732 1417 2385 2576 33
Size: 639 60 685 968 191 1146
Fragments arranged by size:
1146 968 685 639 191 60
SfiI GGCCn_nnn'nGGCC
Cuts at: 628 628
Size: 3689
SfoI GGC'GCC
Cuts at: 1121 1369 1121
Size: 248 3441
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3689
SmaI CCC'GGG
Cuts at: 711 711
Size: 3689
SmlI C'TyrA_G
Cuts at: 844 1759 2473 2735 844
Size: 915 714 262 1798
Fragments arranged by size:
1798 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3689
SphI G_CATG'C
Cuts at: 742 1242 742
Size: 500 3189
StuI AGG'CCT
Cuts at: 599 599
Size: 3689
StyI C'CwwG_G
Cuts at: 545 823 1177 545
Size: 278 354 3057
Fragments arranged by size:
3057 354 278
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 447 604 1272 1405 1864 2073 447
Size: 157 668 133 459 209 2063
Fragments arranged by size:
2063 668 459 209 157 133
TatI w'GTAC_w
Cuts at: 200 3204 200
Size: 3004 685
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 422 454 3206 3238 422
Size: 32 2752 32 873
Fragments arranged by size:
2752 873 32 32
Tth111I GACn'n_nGTC
Cuts at: 1486 1486
Size: 3689
XbaI T'CTAG_A
Cuts at: 720 720
Size: 3689
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 123 240 441 1184 123
Size: 117 201 743 2628
Fragments arranged by size:
2628 743 201 117
XmaI C'CCGG_G
Cuts at: 709 709
Size: 3689
ZraI GAC'GTC
Cuts at: 336 336
Size: 3689
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PfoI PmeI PpiI PshAI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BfrBI BmgBI BmtI BsaXI
BsgI BsiWI BsmI BspEI BsrGI BstEII BstZ17I Bsu36I
ClaI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PpuMI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI