Restriction map for
pF1KB8464
(Circular) (Six-base) MAPSORT of: pF1KB8464.seq Check: 4759 from: 1 to: 5492
pF1KB8464 5492 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2271 2271
Size: 5492
AccI GT'mk_AC
Cuts at: 2530 2530
Size: 5492
Acc65I G'GTAC_C
Cuts at: 2508 2508
Size: 5492
AclI AA'CG_TT
Cuts at: 5332 5332
Size: 5492
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1775 1814 2182 3317 3749 4102 4764 1775
Size: 39 368 1135 432 353 662 2503
Fragments arranged by size:
2503 1135 662 432 368 353 39
AfeI AGC'GCT
Cuts at: 1045 1045
Size: 5492
AflIII A'CryG_T
Cuts at: 2844 4644 4925 2844
Size: 1800 281 3411
Fragments arranged by size:
3411 1800 281
AleI CACnn'nnGTG
Cuts at: 1831 1831
Size: 5492
AlwNI CAG_nnn'CTG
Cuts at: 489 651 1321 2286 2797 4235 489
Size: 162 670 965 511 1438 1746
Fragments arranged by size:
1746 1438 965 670 511 162
ApaI G_GGCC'C
Cuts at: 1026 1026
Size: 5492
ApaLI G'TGCA_C
Cuts at: 4330 4330
Size: 5492
ApoI r'AATT_y
Cuts at: 304 859 2496 4845 5101 304
Size: 555 1637 2349 256 695
Fragments arranged by size:
2349 1637 695 555 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5492
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5492
AvaI C'yCGr_G
Cuts at: 815 2512 815
Size: 1697 3795
BamHI G'GATC_C
Cuts at: 317 2517 317
Size: 2200 3292
BanI G'GyrC_C
Cuts at: 132 641 1713 1992 2082 2238 2508 2922 3170
Size: 509 1072 279 90 156 270 414 248
Cuts at: 3170 3205 132
Size: 35 2419
Fragments arranged by size:
2419 1072 509 414 279 270 248 156
90 35
BanII G_rGCy'C
Cuts at: 164 1026 2147 2506 3536 164
Size: 862 1121 359 1030 2120
Fragments arranged by size:
2120 1121 1030 862 359
BbeI G_GCGC'C
Cuts at: 645 2926 3174 645
Size: 2281 248 2963
Fragments arranged by size:
2963 2281 248
BbsI GAAGACnn'nnnn_
Cuts at: 2170 2283 2170
Size: 113 5379
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1417 1451 90
Size: 34 1293 34 4131
Fragments arranged by size:
4131 1293 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3390 4446 5492 3390
Size: 1056 1046 3390
Fragments arranged by size:
3390 1056 1046
BclI T'GATC_A
Cuts at: 3012 3012
Size: 5492
BglI GCCn_nnn'nGGC
Cuts at: 112 1625 112
Size: 1513 3979
BglII A'GATC_T
Cuts at: 758 3007 5044 758
Size: 2249 2037 1206
Fragments arranged by size:
2249 2037 1206
BlpI GC'TnA_GC
Cuts at: 1517 2604 1517
Size: 1087 4405
Bme1580I G_kGCm'C
Cuts at: 137 155 536 1026 1214 1838 1997 2243 3117
Size: 18 381 490 188 624 159 246 874
Cuts at: 3117 3210 4334 137
Size: 93 1124 1295
Fragments arranged by size:
1295 1124 874 624 490 381 246 188
159 93 18
BmrI ACTGGGnnnn_n'
Cuts at: 1364 2704 2785 3120 5264 1364
Size: 1340 81 335 2144 1592
Fragments arranged by size:
2144 1592 1340 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2166 3859 2166
Size: 1693 3799
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 1241 1298 1310 1444 1657 1844 2081 230
Size: 1011 57 12 134 213 187 237 3641
Fragments arranged by size:
3641 1011 237 213 187 134 57 12
Bpu10I CC'TnA_GC
Cuts at: 425 425
Size: 5492
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 778 2446 2668 3547 4261 4559 778
Size: 1668 222 879 714 298 1711
Fragments arranged by size:
1711 1668 879 714 298 222
BsaI GGTCTCn'nnnn_
Cuts at: 517 2322 5196 517
Size: 1805 2874 813
Fragments arranged by size:
2874 1805 813
BsaAI yAC'GTr
Cuts at: 2845 2845
Size: 5492
BsaBI GATnn'nnATC
Cuts at: 6 1286 1334 3006 5043 6
Size: 1280 48 1672 2037 455
Fragments arranged by size:
2037 1672 1280 455 48
BsaHI Gr'CG_yC
Cuts at: 642 2923 3171 3873 5369 642
Size: 2281 248 702 1496 765
Fragments arranged by size:
2281 1496 765 702 248
BsaWI w'CCGG_w
Cuts at: 2681 3202 3880 4291 4438 4712 2681
Size: 521 678 411 147 274 3461
Fragments arranged by size:
3461 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1075 1105 1075
Size: 30 5462
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 233 536 907 1027 1114 1141 1591 1594 2203
Size: 303 371 120 87 27 450 3 609
Cuts at: 2203 2206 233
Size: 3 3519
Fragments arranged by size:
3519 609 450 371 303 120 87 27
3 3
BseYI C'CCAG_C
Cuts at: 479 656 1017 1139 1643 1747 1807 2091 2917
Size: 177 361 122 504 104 60 284 826
Cuts at: 2917 3675 4340 4748 479
Size: 758 665 408 1223
Fragments arranged by size:
1223 826 758 665 504 408 361 284
177 122 104 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 191 145
Size: 46 5446
BsiEI CG_ry'CG
Cuts at: 104 116 3034 3080 4310 104
Size: 12 2918 46 1230 1286
Fragments arranged by size:
2918 1286 1230 46 12
BsiHKAI G_wGCw'C
Cuts at: 2506 3284 3474 4334 2506
Size: 778 190 860 3664
Fragments arranged by size:
3664 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 137 155 164 536 1026 1214 1838 1997 2147
Size: 18 9 372 490 188 624 159 150
Cuts at: 2147 2243 2506 3117 3210 3284 3474 3536 4334
Size: 96 263 611 93 74 190 62 798
Cuts at: 4334 137
Size: 1295
Fragments arranged by size:
1295 798 624 611 490 372 263 190
188 159 150 96 93 74 62 18
9
BspHI T'CATG_A
Cuts at: 2340 3924 2340
Size: 1584 3908
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2271 2542 2756 3058 3439 2271
Size: 271 214 302 381 4324
Fragments arranged by size:
4324 381 302 271 214
BsrBI CCG'CTC
Cuts at: 50 909 1521 3784 3838 5322 5487 50
Size: 859 612 2263 54 1484 165 55
Fragments arranged by size:
2263 1484 859 612 165 55 54
BsrDI GCAATG_nn'
Cuts at: 563 3404 563
Size: 2841 2651
BsrFI r'CCGG_y
Cuts at: 111 3490 3671 111
Size: 3379 181 1932
Fragments arranged by size:
3379 1932 181
BssSI C'ACGA_G
Cuts at: 3763 4471 3763
Size: 708 4784
BstAPI GCAn_nnn'nTGC
Cuts at: 132 489 1209 2869 132
Size: 357 720 1660 2755
Fragments arranged by size:
2755 1660 720 357
BstBI TT'CG_AA
Cuts at: 3853 3853
Size: 5492
BstEII G'GTnAC_C
Cuts at: 1605 1605
Size: 5492
BstXI CCAn_nnnn'nTGG
Cuts at: 285 1710 2415 285
Size: 1425 705 3362
Fragments arranged by size:
3362 1425 705
BstYI r'GATC_y
Cuts at: 1 317 758 1364 1583 2517 2984 3007 3342
Size: 316 441 606 219 934 467 23 335
Cuts at: 3342 3588 3894 3906 3992 5044 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 934 606 467 449 441 335 316
306 246 219 86 23 12
BtgI C'CryG_G
Cuts at: 155 2132 155
Size: 1977 3515
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1475 3442 3623 3786 1475
Size: 1967 181 163 3181
Fragments arranged by size:
3181 1967 181 163
BtsI GCAGTG_nn'
Cuts at: 2735 2735
Size: 5492
DraI TTT'AAA
Cuts at: 2491 2491
Size: 5492
DrdI GACnn_nn'nnGTC
Cuts at: 3198 4542 3198
Size: 1344 4148
EaeI y'GGCC_r
Cuts at: 113 287 629 1617 2432 3077 3251 3642 3669
Size: 174 342 988 815 645 174 391 27
Cuts at: 3669 5014 113
Size: 1345 591
Fragments arranged by size:
1345 988 815 645 591 391 342 174
174 27
EagI C'GGCC_G
Cuts at: 113 3077 113
Size: 2964 2528
EarI CTCTTCn'nnn_
Cuts at: 724 884 3515 3725 724
Size: 160 2631 210 2491
Fragments arranged by size:
2631 2491 210 160
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 426 691 1138 3700 4442 4588 5302 426
Size: 265 447 2562 742 146 714 616
Fragments arranged by size:
2562 742 714 616 447 265 146
EcoICRI GAG'CTC
Cuts at: 2504 2504
Size: 5492
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 1241 1298 1310 1444 1657 1775 1814 1844
Size: 1011 57 12 134 213 118 39 30
Cuts at: 1844 2081 2182 3317 3749 4102 4764 230
Size: 237 101 1135 432 353 662 958
Fragments arranged by size:
1135 1011 958 662 432 353 237 213
134 118 101 57 39 30 12
EcoNI CCTnn'n_nnAGG
Cuts at: 1036 1036
Size: 5492
EcoO109I rG'GnC_Cy
Cuts at: 1022 2631 1022
Size: 1609 3883
EcoRI G'AATT_C
Cuts at: 2496 2496
Size: 5492
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 369 3511 369
Size: 3142 2350
FspI TGC'GCA
Cuts at: 1396 2203 3273 1396
Size: 807 1070 3615
Fragments arranged by size:
3615 1070 807
FspAI rTGC'GCAy
Cuts at: 1396 1396
Size: 5492
HaeII r_GCGC'y
Cuts at: 645 1047 2926 3174 4404 5175 645
Size: 402 1879 248 1230 771 962
Fragments arranged by size:
1879 1230 962 771 402 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 562 594 1075 1107 1606 1638 2302 2334 2395
Size: 32 481 32 499 32 664 32 61
Cuts at: 2395 2427 3334 3364 3366 3396 562
Size: 32 907 30 2 30 2658
Fragments arranged by size:
2658 907 664 499 481 61 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 267 2531 267
Size: 2264 3228
KasI G'GCGC_C
Cuts at: 641 2922 3170 641
Size: 2281 248 2963
Fragments arranged by size:
2963 2281 248
KpnI G_GTAC'C
Cuts at: 2512 2512
Size: 5492
MluI A'CGCG_T
Cuts at: 4925 4925
Size: 5492
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 345 993 2432 4251 4435 345
Size: 648 1439 1819 184 1402
Fragments arranged by size:
1819 1439 1402 648 184
MscI TGG'CCA
Cuts at: 289 631 1619 2434 3253 289
Size: 342 988 815 819 2528
Fragments arranged by size:
2528 988 819 815 342
MslI CAynn'nnrTG
Cuts at: 1831 2047 2329 2353 3481 3608 1831
Size: 216 282 24 1128 127 3715
Fragments arranged by size:
3715 1128 282 216 127 24
MspA1I CmG'CkG
Cuts at: 489 660 1143 1431 2603 2917 3277 4061 4306
Size: 171 483 288 1172 314 360 784 245
Cuts at: 4306 489
Size: 1675
Fragments arranged by size:
1675 1172 784 483 360 314 288 245
171
NaeI GCC'GGC
Cuts at: 113 3673 113
Size: 3560 1932
NarI GG'CG_CC
Cuts at: 642 2923 3171 642
Size: 2281 248 2963
Fragments arranged by size:
2963 2281 248
NcoI C'CATG_G
Cuts at: 2132 2132
Size: 5492
NgoMIV G'CCGG_C
Cuts at: 111 3671 111
Size: 3560 1932
NspI r_CATG'y
Cuts at: 1395 2250 2358 2545 3045 4648 1395
Size: 855 108 187 500 1603 2239
Fragments arranged by size:
2239 1603 855 500 187 108
PciI A'CATG_T
Cuts at: 4644 4644
Size: 5492
PfoI T'CCnGG_A
Cuts at: 863 1673 863
Size: 810 4682
PmeI GTTT'AAAC
Cuts at: 2491 2491
Size: 5492
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 544 576 2179 2211 3938 3970 544
Size: 32 1603 32 1727 32 2066
Fragments arranged by size:
2066 1727 1603 32 32 32
PspOMI G'GGCC_C
Cuts at: 1022 1022
Size: 5492
PstI C_TGCA'G
Cuts at: 2364 2539 3224 2364
Size: 175 685 4632
Fragments arranged by size:
4632 685 175
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5492
PvuII CAG'CTG
Cuts at: 489 660 1143 2917 3277 489
Size: 171 483 1774 360 2704
Fragments arranged by size:
2704 1774 483 360 171
RsrII CG'GwC_CG
Cuts at: 3687 3687
Size: 5492
SacI G_AGCT'C
Cuts at: 2506 2506
Size: 5492
SalI G'TCGA_C
Cuts at: 2529 2529
Size: 5492
SapI GCTCTTCn'nnn_
Cuts at: 3515 3725 3515
Size: 210 5282
SbfI CC_TGCA'GG
Cuts at: 2364 2539 2364
Size: 175 5317
ScaI AGT'ACT
Cuts at: 5009 5009
Size: 5492
SexAI A'CCwGG_T
Cuts at: 1479 2312 1479
Size: 833 4659
SfcI C'TryA_G
Cuts at: 33 1871 2360 2377 2535 3220 4188 4379 33
Size: 1838 489 17 158 685 968 191 1146
Fragments arranged by size:
1838 1146 968 685 489 191 158 17
SfoI GGC'GCC
Cuts at: 643 2924 3172 643
Size: 2281 248 2963
Fragments arranged by size:
2963 2281 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5492
SmaI CCC'GGG
Cuts at: 2514 2514
Size: 5492
SmlI C'TyrA_G
Cuts at: 793 2461 2647 3562 4276 4538 793
Size: 1668 186 915 714 262 1747
Fragments arranged by size:
1747 1668 915 714 262 186
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5492
SphI G_CATG'C
Cuts at: 2358 2545 3045 2358
Size: 187 500 4805
Fragments arranged by size:
4805 500 187
SspI AAT'ATT
Cuts at: 261 261
Size: 5492
StuI AGG'CCT
Cuts at: 1709 1709
Size: 5492
StyI C'CwwG_G
Cuts at: 449 2132 2626 2980 449
Size: 1683 494 354 2961
Fragments arranged by size:
2961 1683 494 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 493 786 1171 1245 1836 1860 3075 3208 3667
Size: 293 385 74 591 24 1215 133 459
Cuts at: 3667 3876 493
Size: 209 2109
Fragments arranged by size:
2109 1215 591 459 385 293 209 133
74 24
TatI w'GTAC_w
Cuts at: 749 1161 5007 749
Size: 412 3846 1234
Fragments arranged by size:
3846 1234 412
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 820 852 5009 5041 820
Size: 32 4157 32 1271
Fragments arranged by size:
4157 1271 32 32
Tth111I GACn'n_nGTC
Cuts at: 1264 2314 3289 1264
Size: 1050 975 3467
Fragments arranged by size:
3467 1050 975
XbaI T'CTAG_A
Cuts at: 2523 2523
Size: 5492
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1986 2987 1986
Size: 1001 4491
XmaI C'CCGG_G
Cuts at: 2512 2512
Size: 5492
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AgeI AhdI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI BsiWI BsmI BsmBI
BspEI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SwaI XhoI XmnI ZraI