Restriction map for
pF1KB8465
(Circular) (Six-base) MAPSORT of: pF1KB8465.seq Check: 8171 from: 1 to: 3491
pF1KB8465 3491 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 290 529 290
Size: 239 3252
Acc65I G'GTAC_C
Cuts at: 507 507
Size: 3491
AclI AA'CG_TT
Cuts at: 3331 3331
Size: 3491
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 1316 1748 2101 2763 358
Size: 958 432 353 662 1086
Fragments arranged by size:
1086 958 662 432 353
AflIII A'CryG_T
Cuts at: 163 843 2643 2924 163
Size: 680 1800 281 730
Fragments arranged by size:
1800 730 680 281
AgeI A'CCGG_T
Cuts at: 178 178
Size: 3491
AlwNI CAG_nnn'CTG
Cuts at: 391 796 2234 391
Size: 405 1438 1648
Fragments arranged by size:
1648 1438 405
ApaLI G'TGCA_C
Cuts at: 2329 2329
Size: 3491
ApoI r'AATT_y
Cuts at: 495 2844 3100 495
Size: 2349 256 886
Fragments arranged by size:
2349 886 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3491
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3491
AvaI C'yCGr_G
Cuts at: 511 511
Size: 3491
BamHI G'GATC_C
Cuts at: 516 516
Size: 3491
BanI G'GyrC_C
Cuts at: 507 921 1169 1204 507
Size: 414 248 35 2794
Fragments arranged by size:
2794 414 248 35
BanII G_rGCy'C
Cuts at: 505 1535 505
Size: 1030 2461
BbeI G_GCGC'C
Cuts at: 925 1173 925
Size: 248 3243
BciVI GTATCCnnnnn_n'
Cuts at: 1389 2445 3491 1389
Size: 1056 1046 1389
Fragments arranged by size:
1389 1056 1046
BclI T'GATC_A
Cuts at: 1011 1011
Size: 3491
BglI GCCn_nnn'nGGC
Cuts at: 427 427
Size: 3491
BglII A'GATC_T
Cuts at: 1006 3043 1006
Size: 2037 1454
BlpI GC'TnA_GC
Cuts at: 603 603
Size: 3491
Bme1580I G_kGCm'C
Cuts at: 1116 1209 2333 1116
Size: 93 1124 2274
Fragments arranged by size:
2274 1124 93
BmgBI CAC'GTC
Cuts at: 448 460 448
Size: 12 3479
BmrI ACTGGGnnnn_n'
Cuts at: 703 784 1119 3263 703
Size: 81 335 2144 931
Fragments arranged by size:
2144 931 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1858 1858
Size: 3491
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 301
Size: 3491
Bpu10I CC'TnA_GC
Cuts at: 384 384
Size: 3491
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 667 1546 2260 2558 400
Size: 267 879 714 298 1333
Fragments arranged by size:
1333 879 714 298 267
BsaI GGTCTCn'nnnn_
Cuts at: 3195 3195
Size: 3491
BsaAI yAC'GTr
Cuts at: 844 844
Size: 3491
BsaBI GATnn'nnATC
Cuts at: 6 1005 3042 6
Size: 999 2037 455
Fragments arranged by size:
2037 999 455
BsaHI Gr'CG_yC
Cuts at: 922 1170 1872 3368 922
Size: 248 702 1496 1045
Fragments arranged by size:
1496 1045 702 248
BsaWI w'CCGG_w
Cuts at: 178 680 1201 1879 2290 2437 2711 178
Size: 502 521 678 411 147 274 958
Fragments arranged by size:
958 678 521 502 411 274 147
BseYI C'CCAG_C
Cuts at: 916 1674 2339 2747 916
Size: 758 665 408 1660
Fragments arranged by size:
1660 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1033 1079 2309 104
Size: 929 46 1230 1286
Fragments arranged by size:
1286 1230 929 46
BsiHKAI G_wGCw'C
Cuts at: 505 1283 1473 2333 505
Size: 778 190 860 1663
Fragments arranged by size:
1663 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 163 163
Size: 3491
Bsp1286I G_dGCh'C
Cuts at: 505 1116 1209 1283 1473 1535 2333 505
Size: 611 93 74 190 62 798 1663
Fragments arranged by size:
1663 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 1923 1923
Size: 3491
BspMI ACCTGCnnnn'nnnn_
Cuts at: 276 541 755 1057 1438 276
Size: 265 214 302 381 2329
Fragments arranged by size:
2329 381 302 265 214
BsrBI CCG'CTC
Cuts at: 50 1783 1837 3321 3486 50
Size: 1733 54 1484 165 55
Fragments arranged by size:
1733 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1403 1403
Size: 3491
BsrFI r'CCGG_y
Cuts at: 178 1489 1670 178
Size: 1311 181 1999
Fragments arranged by size:
1999 1311 181
BssSI C'ACGA_G
Cuts at: 1762 2470 1762
Size: 708 2783
BstAPI GCAn_nnn'nTGC
Cuts at: 868 868
Size: 3491
BstBI TT'CG_AA
Cuts at: 1852 1852
Size: 3491
BstYI r'GATC_y
Cuts at: 1 516 983 1006 1341 1587 1893 1905 1991
Size: 515 467 23 335 246 306 12 86
Cuts at: 1991 3043 1
Size: 1052 449
Fragments arranged by size:
1052 515 467 449 335 306 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 291 291
Size: 3491
BtgI C'CryG_G
Cuts at: 297 297
Size: 3491
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1441 1622 1785 1441
Size: 181 163 3147
Fragments arranged by size:
3147 181 163
BtsI GCAGTG_nn'
Cuts at: 734 734
Size: 3491
DraI TTT'AAA
Cuts at: 490 490
Size: 3491
DraIII CAC_nnn'GTG
Cuts at: 159 159
Size: 3491
DrdI GACnn_nn'nnGTC
Cuts at: 1197 2541 1197
Size: 1344 2147
EaeI y'GGCC_r
Cuts at: 264 393 428 1076 1250 1641 1668 3013 264
Size: 129 35 648 174 391 27 1345 742
Fragments arranged by size:
1345 742 648 391 174 129 35 27
EagI C'GGCC_G
Cuts at: 1076 1076
Size: 3491
EarI CTCTTCn'nnn_
Cuts at: 435 477 1514 1724 435
Size: 42 1037 210 2202
Fragments arranged by size:
2202 1037 210 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1699 2441 2587 3301 1699
Size: 742 146 714 1889
Fragments arranged by size:
1889 742 714 146
EcoICRI GAG'CTC
Cuts at: 503 503
Size: 3491
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 358 1316 1748 2101 2763 301
Size: 57 958 432 353 662 1029
Fragments arranged by size:
1029 958 662 432 353 57
EcoO109I rG'GnC_Cy
Cuts at: 630 630
Size: 3491
EcoRI G'AATT_C
Cuts at: 495 495
Size: 3491
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1510 1510
Size: 3491
FspI TGC'GCA
Cuts at: 1272 1272
Size: 3491
HaeII r_GCGC'y
Cuts at: 925 1173 2403 3174 925
Size: 248 1230 771 1242
Fragments arranged by size:
1242 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1333 1363 1365 1395 1333
Size: 30 2 30 3429
Fragments arranged by size:
3429 30 30 2
HincII GTy'rAC
Cuts at: 530 530
Size: 3491
KasI G'GCGC_C
Cuts at: 921 1169 921
Size: 248 3243
KpnI G_GTAC'C
Cuts at: 511 511
Size: 3491
MluI A'CGCG_T
Cuts at: 2924 2924
Size: 3491
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 263 2250 2434 263
Size: 1987 184 1320
Fragments arranged by size:
1987 1320 184
MscI TGG'CCA
Cuts at: 1252 1252
Size: 3491
MslI CAynn'nnrTG
Cuts at: 1480 1607 1480
Size: 127 3364
MspA1I CmG'CkG
Cuts at: 279 602 916 1276 2060 2305 279
Size: 323 314 360 784 245 1465
Fragments arranged by size:
1465 784 360 323 314 245
NaeI GCC'GGC
Cuts at: 1672 1672
Size: 3491
NarI GG'CG_CC
Cuts at: 922 1170 922
Size: 248 3243
NgoMIV G'CCGG_C
Cuts at: 1670 1670
Size: 3491
NspI r_CATG'y
Cuts at: 167 336 544 1044 2647 167
Size: 169 208 500 1603 1011
Fragments arranged by size:
1603 1011 500 208 169
PciI A'CATG_T
Cuts at: 163 2643 163
Size: 2480 1011
PmeI GTTT'AAAC
Cuts at: 490 490
Size: 3491
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1937 1969 1937
Size: 32 3459
PstI C_TGCA'G
Cuts at: 538 1223 538
Size: 685 2806
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3491
PvuII CAG'CTG
Cuts at: 279 916 1276 279
Size: 637 360 2494
Fragments arranged by size:
2494 637 360
RsrII CG'GwC_CG
Cuts at: 1686 1686
Size: 3491
SacI G_AGCT'C
Cuts at: 505 505
Size: 3491
SalI G'TCGA_C
Cuts at: 528 528
Size: 3491
SapI GCTCTTCn'nnn_
Cuts at: 1514 1724 1514
Size: 210 3281
SbfI CC_TGCA'GG
Cuts at: 538 538
Size: 3491
ScaI AGT'ACT
Cuts at: 134 3008 134
Size: 2874 617
SfcI C'TryA_G
Cuts at: 33 534 1219 2187 2378 33
Size: 501 685 968 191 1146
Fragments arranged by size:
1146 968 685 501 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 427 427
Size: 3491
SfoI GGC'GCC
Cuts at: 923 1171 923
Size: 248 3243
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3491
SgrAI Cr'CCGG_yG
Cuts at: 178 178
Size: 3491
SmaI CCC'GGG
Cuts at: 513 513
Size: 3491
SmlI C'TyrA_G
Cuts at: 415 646 1561 2275 2537 415
Size: 231 915 714 262 1369
Fragments arranged by size:
1369 915 714 262 231
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3491
SphI G_CATG'C
Cuts at: 544 1044 544
Size: 500 2991
StyI C'CwwG_G
Cuts at: 378 625 979 378
Size: 247 354 2890
Fragments arranged by size:
2890 354 247
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 363 1074 1207 1666 1875 363
Size: 711 133 459 209 1979
Fragments arranged by size:
1979 711 459 209 133
TatI w'GTAC_w
Cuts at: 132 3006 132
Size: 2874 617
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3008 3040 3008
Size: 32 3459
Tth111I GACn'n_nGTC
Cuts at: 1288 1288
Size: 3491
XbaI T'CTAG_A
Cuts at: 522 522
Size: 3491
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 986 986
Size: 3491
XmaI C'CCGG_G
Cuts at: 511 511
Size: 3491
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BstZ17I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmtI BsaXI BseRI BsgI BsiWI BsmI BspEI BsrGI
BssHII BstEII BstXI Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI