Restriction map for
pF1KB8471
(Circular) (Six-base) MAPSORT of: pF1KB8471.seq Check: 5922 from: 1 to: 5849
pF1KB8471 5849 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1218 1763 1218
Size: 545 5304
AatII G_ACGT'C
Cuts at: 2168 2738 2168
Size: 570 5279
AccI GT'mk_AC
Cuts at: 2887 2887
Size: 5849
Acc65I G'GTAC_C
Cuts at: 1876 2865 1876
Size: 989 4860
AclI AA'CG_TT
Cuts at: 5689 5689
Size: 5849
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 506 1094 2077 2842 3674 4106 4853 5121 506
Size: 588 983 765 832 432 747 268 1234
Fragments arranged by size:
1234 983 832 765 747 588 432 268
AflIII A'CryG_T
Cuts at: 1421 1859 3201 4305 5282 1421
Size: 438 1342 1104 977 1988
Fragments arranged by size:
1988 1342 1104 977 438
AgeI A'CCGG_T
Cuts at: 4237 4237
Size: 5849
AlwNI CAG_nnn'CTG
Cuts at: 2234 2769 3154 4721 2234
Size: 535 385 1567 3362
Fragments arranged by size:
3362 1567 535 385
ApaI G_GGCC'C
Cuts at: 450 806 1338 2206 2481 450
Size: 356 532 868 275 3818
Fragments arranged by size:
3818 868 532 356 275
ApaLI G'TGCA_C
Cuts at: 2688 4619 2688
Size: 1931 3918
ApoI r'AATT_y
Cuts at: 1111 1954 2853 5202 5458 1111
Size: 843 899 2349 256 1502
Fragments arranged by size:
2349 1502 899 843 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5849
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5849
AvaI C'yCGr_G
Cuts at: 133 605 805 2425 2869 133
Size: 472 200 1620 444 3113
Fragments arranged by size:
3113 1620 472 444 200
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1860 1893 1860
Size: 33 5816
BamHI G'GATC_C
Cuts at: 2874 2874
Size: 5849
BanI G'GyrC_C
Cuts at: 355 1078 1876 2135 2865 3279 3527 3562 355
Size: 723 798 259 730 414 248 35 2642
Fragments arranged by size:
2642 798 730 723 414 259 248 35
BanII G_rGCy'C
Cuts at: 230 404 424 450 543 604 806 899 1153
Size: 174 20 26 93 61 202 93 254
Cuts at: 1153 1338 2048 2206 2481 2863 3893 230
Size: 185 710 158 275 382 1030 2186
Fragments arranged by size:
2186 1030 710 382 275 254 202 185
174 158 93 93 61 26 20
BbeI G_GCGC'C
Cuts at: 359 3283 3531 359
Size: 2924 248 2677
Fragments arranged by size:
2924 2677 248
BbsI GAAGACnn'nnnn_
Cuts at: 1847 2649 1847
Size: 802 5047
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5815
BciVI GTATCCnnnnn_n'
Cuts at: 3747 4508 5849 3747
Size: 761 1341 3747
Fragments arranged by size:
3747 1341 761
BclI T'GATC_A
Cuts at: 3369 3369
Size: 5849
BglI GCCn_nnn'nGGC
Cuts at: 1367 1917 1367
Size: 550 5299
BglII A'GATC_T
Cuts at: 3364 5401 3364
Size: 2037 3812
BlpI GC'TnA_GC
Cuts at: 1147 2961 1147
Size: 1814 4035
Bme1580I G_kGCm'C
Cuts at: 450 806 1338 1871 2206 2287 2481 2692 3474
Size: 356 532 533 335 81 194 211 782
Cuts at: 3474 3567 4623 450
Size: 93 1056 1676
Fragments arranged by size:
1676 1056 782 533 532 356 335 211
194 93 81
BmrI ACTGGGnnnn_n'
Cuts at: 2713 3061 3142 3477 5621 2713
Size: 348 81 335 2144 2941
Fragments arranged by size:
2941 2144 348 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4216 4216
Size: 5849
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 108 1393 1693 1906 2566 2584 108
Size: 1285 300 213 660 18 3373
Fragments arranged by size:
3373 1285 660 300 213 18
Bpu10I CC'TnA_GC
Cuts at: 893 893
Size: 5849
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 477 3025 3904 4396 4694 477
Size: 2548 879 492 298 1632
Fragments arranged by size:
2548 1632 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 469 565 5553 469
Size: 96 4988 765
Fragments arranged by size:
4988 765 96
BsaAI yAC'GTr
Cuts at: 1181 3202 1181
Size: 2021 3828
BsaBI GATnn'nnATC
Cuts at: 6 3363 5400 6
Size: 3357 2037 455
Fragments arranged by size:
3357 2037 455
BsaHI Gr'CG_yC
Cuts at: 356 2165 2735 3280 3528 4230 5726 356
Size: 1809 570 545 248 702 1496 479
Fragments arranged by size:
1809 1496 702 570 545 479 248
BsaWI w'CCGG_w
Cuts at: 3038 3559 4237 4511 4658 5069 3038
Size: 521 678 274 147 411 3818
Fragments arranged by size:
3818 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 979 1009 1666 1696 1897 1927 979
Size: 30 657 30 201 30 4901
Fragments arranged by size:
4901 657 201 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 181 202 211 229 427 551 841 181
Size: 21 9 18 198 124 290 5189
Fragments arranged by size:
5189 290 198 124 21 18 9
BseYI C'CCAG_C
Cuts at: 229 404 427 450 632 913 1085 1986 2772
Size: 175 23 23 182 281 172 901 786
Cuts at: 2772 3274 4032 4609 5105 229
Size: 502 758 577 496 973
Fragments arranged by size:
973 901 786 758 577 502 496 281
182 175 172 23 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 488 501 868 1330 1701 2453 488
Size: 13 367 462 371 752 3884
Fragments arranged by size:
3884 752 462 371 367 13
BsiEI CG_ry'CG
Cuts at: 104 969 3391 3437 4645 104
Size: 865 2422 46 1208 1308
Fragments arranged by size:
2422 1308 1208 865 46
BsiHKAI G_wGCw'C
Cuts at: 1153 1489 1748 2692 2863 3641 3831 4623 1153
Size: 336 259 944 171 778 190 792 2379
Fragments arranged by size:
2379 944 792 778 336 259 190 171
BsmI GAATG_Cn'
Cuts at: 786 930 2069 786
Size: 144 1139 4566
Fragments arranged by size:
4566 1139 144
BsmBI CGTCTCn'nnnn_
Cuts at: 1097 2742 1097
Size: 1645 4204
Bsp1286I G_dGCh'C
Cuts at: 230 404 424 450 543 604 806 899 1153
Size: 174 20 26 93 61 202 93 254
Cuts at: 1153 1338 1489 1748 1871 2048 2206 2287 2481
Size: 185 151 259 123 177 158 81 194
Cuts at: 2481 2692 2863 3474 3567 3641 3831 3893 4623
Size: 211 171 611 93 74 190 62 730
Cuts at: 4623 230
Size: 1456
Fragments arranged by size:
1456 730 611 259 254 211 202 194
190 185 177 174 171 158 151 123
93 93 93 81 74 62 61 26
20
BspEI T'CCGG_A
Cuts at: 5069 5069
Size: 5849
BspHI T'CATG_A
Cuts at: 2660 5025 2660
Size: 2365 3484
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1218 1763 2899 3113 3415 3796 1218
Size: 545 1136 214 302 381 3271
Fragments arranged by size:
3271 1136 545 381 302 214
BsrBI CCG'CTC
Cuts at: 50 162 1539 4141 4195 5679 5844 50
Size: 112 1377 2602 54 1484 165 55
Fragments arranged by size:
2602 1484 1377 165 112 55 54
BsrDI GCAATG_nn'
Cuts at: 767 2087 2244 3761 767
Size: 1320 157 1517 2855
Fragments arranged by size:
2855 1517 1320 157
BsrFI r'CCGG_y
Cuts at: 611 2291 2777 3847 4028 4237 611
Size: 1680 486 1070 181 209 2223
Fragments arranged by size:
2223 1680 1070 486 209 181
BsrGI T'GTAC_A
Cuts at: 1412 1412
Size: 5849
BssSI C'ACGA_G
Cuts at: 4120 4478 4120
Size: 358 5491
BstAPI GCAn_nnn'nTGC
Cuts at: 1664 1724 2234 2769 3226 1664
Size: 60 510 535 457 4287
Fragments arranged by size:
4287 535 510 457 60
BstBI TT'CG_AA
Cuts at: 4210 4210
Size: 5849
BstEII G'GTnAC_C
Cuts at: 2494 2494
Size: 5849
BstXI CCAn_nnnn'nTGG
Cuts at: 594 1302 1357 2121 594
Size: 708 55 764 4322
Fragments arranged by size:
4322 764 708 55
BstYI r'GATC_y
Cuts at: 1 2874 3341 3364 3699 3945 4957 5043 5055
Size: 2873 467 23 335 246 1012 86 12
Cuts at: 5055 5401 1
Size: 346 449
Fragments arranged by size:
2873 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1364 1436 1364
Size: 72 5777
BtgI C'CryG_G
Cuts at: 236 338 899 1301 2387 236
Size: 102 561 402 1086 3698
Fragments arranged by size:
3698 1086 561 402 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 956 1292 3799 3980 4143 956
Size: 336 2507 181 163 2662
Fragments arranged by size:
2662 2507 336 181 163
BtsI GCAGTG_nn'
Cuts at: 506 1167 1289 3092 506
Size: 661 122 1803 3263
Fragments arranged by size:
3263 1803 661 122
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 482 517 482
Size: 35 5814
DraI TTT'AAA
Cuts at: 2848 2848
Size: 5849
DrdI GACnn_nn'nnGTC
Cuts at: 3555 4413 3555
Size: 858 4991
EaeI y'GGCC_r
Cuts at: 966 1024 1232 2289 2384 3434 3608 3999 4026
Size: 58 208 1057 95 1050 174 391 27
Cuts at: 4026 5371 966
Size: 1345 1444
Fragments arranged by size:
1444 1345 1057 1050 391 208 174 95
58 27
EagI C'GGCC_G
Cuts at: 966 3434 966
Size: 2468 3381
EarI CTCTTCn'nnn_
Cuts at: 1382 1477 1636 1990 2302 2815 3872 4082 1382
Size: 95 159 354 312 513 1057 210 3149
Fragments arranged by size:
3149 1057 513 354 312 210 159 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2791 4057 4367 4513 5659 2791
Size: 1266 310 146 1146 2981
Fragments arranged by size:
2981 1266 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1151 2861 1151
Size: 1710 4139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 108 506 1094 1393 1693 1906 2077 2566 2584
Size: 398 588 299 300 213 171 489 18
Cuts at: 2584 2842 3674 4106 4853 5121 108
Size: 258 832 432 747 268 836
Fragments arranged by size:
836 832 747 588 489 432 398 300
299 268 258 213 171 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2278 2278
Size: 5849
EcoO109I rG'GnC_Cy
Cuts at: 309 446 447 687 802 1221 1335 1368 2202
Size: 137 1 240 115 419 114 33 834
Cuts at: 2202 2271 2477 2988 309
Size: 69 206 511 3170
Fragments arranged by size:
3170 834 511 419 240 206 137 115
114 69 33 1
EcoRI G'AATT_C
Cuts at: 2853 2853
Size: 5849
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1683 3868 1683
Size: 2185 3664
FspI TGC'GCA
Cuts at: 3630 3630
Size: 5849
HaeII r_GCGC'y
Cuts at: 222 359 3283 3531 4553 5532 222
Size: 137 2924 248 1022 979 539
Fragments arranged by size:
2924 1022 979 539 248 137
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 853 885 1897 1929 3691 3721 3723 3753 853
Size: 32 1012 32 1762 30 2 30 2949
Fragments arranged by size:
2949 1762 1012 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2256 2888 2256
Size: 632 5217
KasI G'GCGC_C
Cuts at: 355 3279 3527 355
Size: 2924 248 2677
Fragments arranged by size:
2924 2677 248
KpnI G_GTAC'C
Cuts at: 1880 2869 1880
Size: 989 4860
MluI A'CGCG_T
Cuts at: 5282 5282
Size: 5849
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1617 2456 2765 4520 4704 1617
Size: 839 309 1755 184 2762
Fragments arranged by size:
2762 1755 839 309 184
MscI TGG'CCA
Cuts at: 1026 1234 2386 3610 1026
Size: 208 1152 1224 3265
Fragments arranged by size:
3265 1224 1152 208
MslI CAynn'nnrTG
Cuts at: 592 1420 1426 2011 2074 2119 2695 3838 3965
Size: 828 6 585 63 45 576 1143 127
Cuts at: 3965 592
Size: 2476
Fragments arranged by size:
2476 1143 828 585 576 127 63 45
6
MspA1I CmG'CkG
Cuts at: 128 472 654 1072 1085 1146 1444 1492 1990
Size: 344 182 418 13 61 298 48 498
Cuts at: 1990 2467 2820 2960 3274 3634 4647 4892 128
Size: 477 353 140 314 360 1013 245 1085
Fragments arranged by size:
1085 1013 498 477 418 360 353 344
314 298 245 182 140 61 48 13
NaeI GCC'GGC
Cuts at: 613 2293 4030 613
Size: 1680 1737 2432
Fragments arranged by size:
2432 1737 1680
NarI GG'CG_CC
Cuts at: 356 3280 3528 356
Size: 2924 248 2677
Fragments arranged by size:
2924 2677 248
NcoI C'CATG_G
Cuts at: 338 2387 338
Size: 2049 3800
NgoMIV G'CCGG_C
Cuts at: 611 2291 4028 611
Size: 1680 1737 2432
Fragments arranged by size:
2432 1737 1680
NruI TCG'CGA
Cuts at: 2418 2418
Size: 5849
NspI r_CATG'y
Cuts at: 1425 1863 2902 3402 4309 1425
Size: 438 1039 500 907 2965
Fragments arranged by size:
2965 1039 907 500 438
PasI CC'CwG_GG
Cuts at: 1338 1338
Size: 5849
PciI A'CATG_T
Cuts at: 1421 1859 4305 1421
Size: 438 2446 2965
Fragments arranged by size:
2965 2446 438
PfoI T'CCnGG_A
Cuts at: 2192 2192
Size: 5849
PmeI GTTT'AAAC
Cuts at: 2848 2848
Size: 5849
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1613 1645 4988 5020 1613
Size: 32 3343 32 2442
Fragments arranged by size:
3343 2442 32 32
PpuMI rG'GwC_Cy
Cuts at: 309 687 1221 309
Size: 378 534 4937
Fragments arranged by size:
4937 534 378
PspOMI G'GGCC_C
Cuts at: 446 802 1334 2202 2477 446
Size: 356 532 868 275 3818
Fragments arranged by size:
3818 868 532 356 275
PstI C_TGCA'G
Cuts at: 549 1229 1774 2555 2896 3581 549
Size: 680 545 781 341 685 2817
Fragments arranged by size:
2817 781 685 680 545 341
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5849
PvuII CAG'CTG
Cuts at: 472 1072 1444 1990 2467 3274 3634 472
Size: 600 372 546 477 807 360 2687
Fragments arranged by size:
2687 807 600 546 477 372 360
RsrII CG'GwC_CG
Cuts at: 4044 4044
Size: 5849
SacI G_AGCT'C
Cuts at: 1153 2863 1153
Size: 1710 4139
SalI G'TCGA_C
Cuts at: 2886 2886
Size: 5849
SanDI GG'GwC_CC
Cuts at: 687 687
Size: 5849
SapI GCTCTTCn'nnn_
Cuts at: 1477 2302 3872 4082 1477
Size: 825 1570 210 3244
Fragments arranged by size:
3244 1570 825 210
SbfI CC_TGCA'GG
Cuts at: 1229 2896 1229
Size: 1667 4182
ScaI AGT'ACT
Cuts at: 5366 5366
Size: 5849
SfcI C'TryA_G
Cuts at: 33 545 1225 1770 2039 2551 2892 3577 4570
Size: 512 680 545 269 512 341 685 993
Cuts at: 4570 4761 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 680 545 512 512 341
269 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1367 1367
Size: 5849
SfoI GGC'GCC
Cuts at: 357 3281 3529 357
Size: 2924 248 2677
Fragments arranged by size:
2924 2677 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5849
SmaI CCC'GGG
Cuts at: 135 607 807 2871 135
Size: 472 200 2064 3113
Fragments arranged by size:
3113 2064 472 200
SmlI C'TyrA_G
Cuts at: 492 2425 3004 3919 4411 4673 492
Size: 1933 579 915 492 262 1668
Fragments arranged by size:
1933 1668 915 579 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5849
SphI G_CATG'C
Cuts at: 2902 3402 2902
Size: 500 5349
StyI C'CwwG_G
Cuts at: 338 524 761 1295 1502 2336 2387 2983 3337
Size: 186 237 534 207 834 51 596 354
Cuts at: 3337 338
Size: 2850
Fragments arranged by size:
2850 834 596 534 354 237 207 186
51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 188 1416 3432 3565 4024 4233 188
Size: 1228 2016 133 459 209 1804
Fragments arranged by size:
2016 1804 1228 459 209 133
TatI w'GTAC_w
Cuts at: 1412 5364 1412
Size: 3952 1897
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 979 1011 5366 5398 979
Size: 32 4355 32 1430
Fragments arranged by size:
4355 1430 32 32
Tth111I GACn'n_nGTC
Cuts at: 3646 3646
Size: 5849
XbaI T'CTAG_A
Cuts at: 2880 2880
Size: 5849
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 381 3344 381
Size: 2963 2886
XhoI C'TCGA_G
Cuts at: 2425 2425
Size: 5849
XmaI C'CCGG_G
Cuts at: 133 605 805 2869 133
Size: 472 200 2064 3113
Fragments arranged by size:
3113 2064 472 200
XmnI GAAnn'nnTTC
Cuts at: 1255 1255
Size: 5849
ZraI GAC'GTC
Cuts at: 2166 2736 2166
Size: 570 5279
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NspI PasI PciI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI AscI AvrII
BbvCI BfrBI BmgBI BmtI BsiWI BssHII BstZ17I ClaI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NsiI PacI PflMI PmlI PshAI PsiI
PspXI PsrI SacII SexAI SgrAI SnaBI SrfI SspI
StuI SwaI