Restriction map for
pF1KB8472
(Circular) (Six-base) MAPSORT of: pF1KB8472.seq Check: 7801 from: 1 to: 5633
pF1KB8472 5633 bp
With 182 enzymes: SgfI *
June 19, 2019 14:13 ..
AccI GT'mk_AC
Cuts at: 710 2671 710
Size: 1961 3672
Acc65I G'GTAC_C
Cuts at: 2649 2649
Size: 5633
AclI AA'CG_TT
Cuts at: 5473 5473
Size: 5633
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 935 1358 1766 2204 3458 3890 4637 4905
Size: 433 423 408 438 1254 432 747 268
Cuts at: 4905 502
Size: 1230
Fragments arranged by size:
1254 1230 747 438 433 432 423 408
268
AflII C'TTAA_G
Cuts at: 505 505
Size: 5633
AflIII A'CryG_T
Cuts at: 1695 2985 4089 5066 1695
Size: 1290 1104 977 2262
Fragments arranged by size:
2262 1290 1104 977
AgeI A'CCGG_T
Cuts at: 4021 4021
Size: 5633
AhdI GACnn_n'nnGTC
Cuts at: 876 876
Size: 5633
AleI CACnn'nnGTG
Cuts at: 839 2581 839
Size: 1742 3891
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 476 476
Size: 5633
AlwNI CAG_nnn'CTG
Cuts at: 1477 1744 2938 4505 1477
Size: 267 1194 1567 2605
Fragments arranged by size:
2605 1567 1194 267
ApaLI G'TGCA_C
Cuts at: 4403 4403
Size: 5633
ApoI r'AATT_y
Cuts at: 438 811 847 1021 1174 1279 1326 2637 4986
Size: 373 36 174 153 105 47 1311 2349
Cuts at: 4986 5242 438
Size: 256 829
Fragments arranged by size:
2349 1311 829 373 256 174 153 105
47 36
AseI AT'TA_AT
Cuts at: 20 616 20
Size: 596 5037
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5633
AvaI C'yCGr_G
Cuts at: 1226 2653 1226
Size: 1427 4206
BamHI G'GATC_C
Cuts at: 1771 2385 2658 1771
Size: 614 273 4746
Fragments arranged by size:
4746 614 273
BanI G'GyrC_C
Cuts at: 2649 3063 3311 3346 2649
Size: 414 248 35 4936
Fragments arranged by size:
4936 414 248 35
BanII G_rGCy'C
Cuts at: 2647 3677 2647
Size: 1030 4603
BbeI G_GCGC'C
Cuts at: 3067 3315 3067
Size: 248 5385
BbsI GAAGACnn'nnnn_
Cuts at: 1831 2105 1831
Size: 274 5359
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1161 1195 1161
Size: 34 5599
BciVI GTATCCnnnnn_n'
Cuts at: 3531 4292 5633 3531
Size: 761 1341 3531
Fragments arranged by size:
3531 1341 761
BclI T'GATC_A
Cuts at: 1919 2277 3153 1919
Size: 358 876 4399
Fragments arranged by size:
4399 876 358
BglI GCCn_nnn'nGGC
Cuts at: 1813 1813
Size: 5633
BglII A'GATC_T
Cuts at: 954 3148 5185 954
Size: 2194 2037 1402
Fragments arranged by size:
2194 2037 1402
BlpI GC'TnA_GC
Cuts at: 2561 2745 2561
Size: 184 5449
Bme1580I G_kGCm'C
Cuts at: 3258 3351 4407 3258
Size: 93 1056 4484
Fragments arranged by size:
4484 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2845 2926 3261 5405 2845
Size: 81 335 2144 3073
Fragments arranged by size:
3073 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1903 4000 1903
Size: 2097 3536
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1048 1316 1512 1957 1048
Size: 268 196 445 4724
Fragments arranged by size:
4724 445 268 196
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 910 1453 1748 2809 3688 4180 4478 910
Size: 543 295 1061 879 492 298 2065
Fragments arranged by size:
2065 1061 879 543 492 298 295
BsaI GGTCTCn'nnnn_
Cuts at: 1750 5337 1750
Size: 3587 2046
BsaAI yAC'GTr
Cuts at: 2986 2986
Size: 5633
BsaBI GATnn'nnATC
Cuts at: 6 3147 5184 6
Size: 3141 2037 455
Fragments arranged by size:
3141 2037 455
BsaHI Gr'CG_yC
Cuts at: 3064 3312 4014 5510 3064
Size: 248 702 1496 3187
Fragments arranged by size:
3187 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2822 3343 4021 4295 4442 4853 2822
Size: 521 678 274 147 411 3602
Fragments arranged by size:
3602 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2257 2287 2257
Size: 30 5603
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1442 1502 1642 1442
Size: 60 140 5433
Fragments arranged by size:
5433 140 60
BseYI C'CCAG_C
Cuts at: 3058 3816 4393 4889 3058
Size: 758 577 496 3802
Fragments arranged by size:
3802 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 192 403 192
Size: 211 5422
BsiEI CG_ry'CG
Cuts at: 104 204 3175 3221 4429 104
Size: 100 2971 46 1208 1308
Fragments arranged by size:
2971 1308 1208 100 46
BsiHKAI G_wGCw'C
Cuts at: 2647 3425 3615 4407 2647
Size: 778 190 792 3873
Fragments arranged by size:
3873 792 778 190
BsmI GAATG_Cn'
Cuts at: 328 1656 328
Size: 1328 4305
Bsp1286I G_dGCh'C
Cuts at: 2647 3258 3351 3425 3615 3677 4407 2647
Size: 611 93 74 190 62 730 3873
Fragments arranged by size:
3873 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4853 4853
Size: 5633
BspHI T'CATG_A
Cuts at: 4809 4809
Size: 5633
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1850 2683 2897 3199 3580 1850
Size: 833 214 302 381 3903
Fragments arranged by size:
3903 833 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1947 3925 3979 5463 5628 50
Size: 1897 1978 54 1484 165 55
Fragments arranged by size:
1978 1897 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1044 1237 2336 3545 1044
Size: 193 1099 1209 3132
Fragments arranged by size:
3132 1209 1099 193
BsrFI r'CCGG_y
Cuts at: 166 213 3631 3812 4021 166
Size: 47 3418 181 209 1778
Fragments arranged by size:
3418 1778 209 181 47
BsrGI T'GTAC_A
Cuts at: 1895 1895
Size: 5633
BssSI C'ACGA_G
Cuts at: 3904 4262 3904
Size: 358 5275
BstAPI GCAn_nnn'nTGC
Cuts at: 3010 3010
Size: 5633
BstBI TT'CG_AA
Cuts at: 3994 3994
Size: 5633
BstXI CCAn_nnnn'nTGG
Cuts at: 114 1836 114
Size: 1722 3911
BstYI r'GATC_y
Cuts at: 1 666 954 1473 1771 2222 2385 2658 3125
Size: 665 288 519 298 451 163 273 467
Cuts at: 3125 3148 3483 3729 4741 4827 4839 5185 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 665 519 467 451 449 346 335
298 288 273 246 163 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1454 1454
Size: 5633
BtgI C'CryG_G
Cuts at: 318 1566 2381 318
Size: 1248 815 3570
Fragments arranged by size:
3570 1248 815
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3583 3764 3927 3583
Size: 181 163 5289
Fragments arranged by size:
5289 181 163
BtsI GCAGTG_nn'
Cuts at: 629 779 1124 1249 1973 2876 629
Size: 150 345 125 724 903 3386
Fragments arranged by size:
3386 903 724 345 150 125
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 618 653 768 803 1986 2021 618
Size: 35 115 35 1183 35 4230
Fragments arranged by size:
4230 1183 115 35 35 35
DraI TTT'AAA
Cuts at: 309 2632 309
Size: 2323 3310
DrdI GACnn_nn'nnGTC
Cuts at: 1360 3339 4197 1360
Size: 1979 858 2796
Fragments arranged by size:
2796 1979 858
EaeI y'GGCC_r
Cuts at: 321 776 3218 3392 3783 3810 5155 321
Size: 455 2442 174 391 27 1345 799
Fragments arranged by size:
2442 1345 799 455 391 174 27
EagI C'GGCC_G
Cuts at: 3218 3218
Size: 5633
EarI CTCTTCn'nnn_
Cuts at: 425 677 1658 1697 3656 3866 425
Size: 252 981 39 1959 210 2192
Fragments arranged by size:
2192 1959 981 252 210 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3841 4151 4297 5443 3841
Size: 310 146 1146 4031
Fragments arranged by size:
4031 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2645 2645
Size: 5633
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 935 1048 1316 1358 1512 1766 1957 2204
Size: 433 113 268 42 154 254 191 247
Cuts at: 2204 3458 3890 4637 4905 502
Size: 1254 432 747 268 1230
Fragments arranged by size:
1254 1230 747 433 432 268 268 254
247 191 154 113 42
EcoO109I rG'GnC_Cy
Cuts at: 2548 2772 2548
Size: 224 5409
EcoRI G'AATT_C
Cuts at: 1326 2637 1326
Size: 1311 4322
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3652 3652
Size: 5633
FspI TGC'GCA
Cuts at: 3414 3414
Size: 5633
HaeII r_GCGC'y
Cuts at: 3067 3315 4337 5316 3067
Size: 248 1022 979 3384
Fragments arranged by size:
3384 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 582 614 862 894 1547 1579 2370 2402 3475
Size: 32 248 32 653 32 791 32 1073
Cuts at: 3475 3505 3507 3537 582
Size: 30 2 30 2678
Fragments arranged by size:
2678 1073 791 653 248 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 522 2155 2672 522
Size: 1633 517 3483
Fragments arranged by size:
3483 1633 517
HindIII A'AGCT_T
Cuts at: 1999 2605 1999
Size: 606 5027
KasI G'GCGC_C
Cuts at: 3063 3311 3063
Size: 248 5385
KpnI G_GTAC'C
Cuts at: 2653 2653
Size: 5633
MfeI C'AATT_G
Cuts at: 248 248
Size: 5633
MluI A'CGCG_T
Cuts at: 5066 5066
Size: 5633
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2250 4304 4488 2250
Size: 2054 184 3395
Fragments arranged by size:
3395 2054 184
MscI TGG'CCA
Cuts at: 778 3394 778
Size: 2616 3017
MslI CAynn'nnrTG
Cuts at: 112 839 1242 2285 2581 3622 3749 112
Size: 727 403 1043 296 1041 127 1996
Fragments arranged by size:
1996 1043 1041 727 403 296 127
MspA1I CmG'CkG
Cuts at: 206 1336 2581 2744 3058 3418 4431 4676 206
Size: 1130 1245 163 314 360 1013 245 1163
Fragments arranged by size:
1245 1163 1130 1013 360 314 245 163
NaeI GCC'GGC
Cuts at: 168 215 3814 168
Size: 47 3599 1987
Fragments arranged by size:
3599 1987 47
NarI GG'CG_CC
Cuts at: 3064 3312 3064
Size: 248 5385
NcoI C'CATG_G
Cuts at: 318 1566 2381 318
Size: 1248 815 3570
Fragments arranged by size:
3570 1248 815
NdeI CA'TA_TG
Cuts at: 372 648 372
Size: 276 5357
NgoMIV G'CCGG_C
Cuts at: 166 213 3812 166
Size: 47 3599 1987
Fragments arranged by size:
3599 1987 47
NspI r_CATG'y
Cuts at: 1699 2686 3186 4093 1699
Size: 987 500 907 3239
Fragments arranged by size:
3239 987 907 500
PciI A'CATG_T
Cuts at: 1695 4089 1695
Size: 2394 3239
PflMI CCAn_nnn'nTGG
Cuts at: 2527 2527
Size: 5633
PfoI T'CCnGG_A
Cuts at: 2174 2174
Size: 5633
PmeI GTTT'AAAC
Cuts at: 2632 2632
Size: 5633
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4772 4804 4772
Size: 32 5601
PspXI vC'TCGA_Gb
Cuts at: 1226 1226
Size: 5633
PstI C_TGCA'G
Cuts at: 636 2363 2680 3365 636
Size: 1727 317 685 2904
Fragments arranged by size:
2904 1727 685 317
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5633
PvuII CAG'CTG
Cuts at: 1336 2581 3058 3418 1336
Size: 1245 477 360 3551
Fragments arranged by size:
3551 1245 477 360
RsrII CG'GwC_CG
Cuts at: 3828 3828
Size: 5633
SacI G_AGCT'C
Cuts at: 2647 2647
Size: 5633
SalI G'TCGA_C
Cuts at: 2670 2670
Size: 5633
SapI GCTCTTCn'nnn_
Cuts at: 425 1697 3656 3866 425
Size: 1272 1959 210 2192
Fragments arranged by size:
2192 1959 1272 210
SbfI CC_TGCA'GG
Cuts at: 2680 2680
Size: 5633
ScaI AGT'ACT
Cuts at: 1833 2439 5150 1833
Size: 606 2711 2316
Fragments arranged by size:
2711 2316 606
SfcI C'TryA_G
Cuts at: 33 392 515 632 1443 1481 2359 2676 3361
Size: 359 123 117 811 38 878 317 685
Cuts at: 3361 4354 4545 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 878 811 685 359 317 191
123 117 38
SfoI GGC'GCC
Cuts at: 3065 3313 3065
Size: 248 5385
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5633
SgrAI Cr'CCGG_yG
Cuts at: 166 166
Size: 5633
SmaI CCC'GGG
Cuts at: 2655 2655
Size: 5633
SmlI C'TyrA_G
Cuts at: 505 925 1226 1432 1763 2788 3703 4195 4457
Size: 420 301 206 331 1025 915 492 262
Cuts at: 4457 505
Size: 1681
Fragments arranged by size:
1681 1025 915 492 420 331 301 262
206
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5633
SphI G_CATG'C
Cuts at: 2686 3186 2686
Size: 500 5133
StuI AGG'CCT
Cuts at: 1438 1438
Size: 5633
StyI C'CwwG_G
Cuts at: 300 318 1566 1775 2381 2767 3121 300
Size: 18 1248 209 606 386 354 2812
Fragments arranged by size:
2812 1248 606 386 354 209 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3216 3349 3808 4017 3216
Size: 133 459 209 4832
Fragments arranged by size:
4832 459 209 133
TatI w'GTAC_w
Cuts at: 1195 1831 1895 2437 5148 1195
Size: 636 64 542 2711 1680
Fragments arranged by size:
2711 1680 636 542 64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5150 5182 5150
Size: 32 5601
Tth111I GACn'n_nGTC
Cuts at: 3430 3430
Size: 5633
XbaI T'CTAG_A
Cuts at: 2664 2664
Size: 5633
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 2472 3128 114
Size: 2358 656 2619
Fragments arranged by size:
2619 2358 656
XhoI C'TCGA_G
Cuts at: 1226 1226
Size: 5633
XmaI C'CCGG_G
Cuts at: 2653 2653
Size: 5633
XmnI GAAnn'nnTTC
Cuts at: 500 500
Size: 5633
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AloI ApaI AscI AvrII BaeI
BbvCI BfrBI BmgBI BmtI Bpu10I BsiWI BsmBI BssHII
BstEII BstZ17I ClaI DraIII EcoNI EcoRV FseI FspAI
HpaI NheI NotI NruI NsiI PacI PasI PmlI
PpuMI PshAI PsiI PspOMI PsrI SacII SanDI SexAI
SfiI SnaBI SrfI SspI SwaI ZraI