Restriction map for
pF1KB8490
(Circular) (Six-base) MAPSORT of: pF1KB8490.seq Check: 6099 from: 1 to: 5690
pF1KB8490 5690 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 270 928 1145 270
Size: 658 217 4815
Fragments arranged by size:
4815 658 217
AatII G_ACGT'C
Cuts at: 2507 2507
Size: 5690
AccI GT'mk_AC
Cuts at: 2507 2728 2507
Size: 221 5469
Acc65I G'GTAC_C
Cuts at: 2706 2706
Size: 5690
AclI AA'CG_TT
Cuts at: 5530 5530
Size: 5690
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 458 1445 2668 3515 3947 4694 4962 458
Size: 987 1223 847 432 747 268 1186
Fragments arranged by size:
1223 1186 987 847 747 432 268
AfeI AGC'GCT
Cuts at: 2086 2086
Size: 5690
AflIII A'CryG_T
Cuts at: 626 3042 4146 5123 626
Size: 2416 1104 977 1193
Fragments arranged by size:
2416 1193 1104 977
AgeI A'CCGG_T
Cuts at: 4078 4078
Size: 5690
AleI CACnn'nnGTG
Cuts at: 1329 1329
Size: 5690
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2686 2686
Size: 5690
AlwNI CAG_nnn'CTG
Cuts at: 149 191 297 330 423 891 2185 2266 2995
Size: 42 106 33 93 468 1294 81 729
Cuts at: 2995 4562 149
Size: 1567 1277
Fragments arranged by size:
1567 1294 1277 729 468 106 93 81
42 33
ApaI G_GGCC'C
Cuts at: 236 567 826 2642 236
Size: 331 259 1816 3284
Fragments arranged by size:
3284 1816 331 259
ApaLI G'TGCA_C
Cuts at: 4460 4460
Size: 5690
ApoI r'AATT_y
Cuts at: 2512 2554 2694 5043 5299 2512
Size: 42 140 2349 256 2903
Fragments arranged by size:
2903 2349 256 140 42
AseI AT'TA_AT
Cuts at: 20 20
Size: 5690
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5690
AvaI C'yCGr_G
Cuts at: 819 1417 2710 819
Size: 598 1293 3799
Fragments arranged by size:
3799 1293 598
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 649 682 649
Size: 33 5657
BamHI G'GATC_C
Cuts at: 2715 2715
Size: 5690
BanI G'GyrC_C
Cuts at: 940 1536 2706 3120 3368 3403 940
Size: 596 1170 414 248 35 3227
Fragments arranged by size:
3227 1170 596 414 248 35
BanII G_rGCy'C
Cuts at: 236 567 731 826 1037 1229 1639 1988 2042
Size: 331 164 95 211 192 410 349 54
Cuts at: 2042 2423 2483 2642 2704 3734 236
Size: 381 60 159 62 1030 2192
Fragments arranged by size:
2192 1030 410 381 349 331 211 192
164 159 95 62 60 54
BbeI G_GCGC'C
Cuts at: 3124 3372 3124
Size: 248 5442
BbsI GAAGACnn'nnnn_
Cuts at: 623 2321 2507 623
Size: 1698 186 3806
Fragments arranged by size:
3806 1698 186
BbvCI CC'TCA_GC
Cuts at: 400 1702 400
Size: 1302 4388
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 800 834 800
Size: 34 5656
BciVI GTATCCnnnnn_n'
Cuts at: 462 2235 3588 4349 5690 462
Size: 1773 1353 761 1341 462
Fragments arranged by size:
1773 1353 1341 761 462
BclI T'GATC_A
Cuts at: 3210 3210
Size: 5690
BfrBI ATG'CAT
Cuts at: 2433 2433
Size: 5690
BglII A'GATC_T
Cuts at: 2651 3205 5242 2651
Size: 554 2037 3099
Fragments arranged by size:
3099 2037 554
BlpI GC'TnA_GC
Cuts at: 602 1036 1091 1223 2802 602
Size: 434 55 132 1579 3490
Fragments arranged by size:
3490 1579 434 132 55
Bme1580I G_kGCm'C
Cuts at: 236 567 678 826 1430 1502 2273 2565 2642
Size: 331 111 148 604 72 771 292 77
Cuts at: 2642 3315 3408 4464 236
Size: 673 93 1056 1462
Fragments arranged by size:
1462 1056 771 673 604 331 292 148
111 93 77 72
BmgBI CAC'GTC
Cuts at: 629 629
Size: 5690
BmrI ACTGGGnnnn_n'
Cuts at: 501 1406 2902 2983 3318 5462 501
Size: 905 1496 81 335 2144 729
Fragments arranged by size:
2144 1496 905 729 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4057 4057
Size: 5690
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 400 1252 1312 1728 2304 2437 370
Size: 30 852 60 416 576 133 3623
Fragments arranged by size:
3623 852 576 416 133 60 30
Bpu10I CC'TnA_GC
Cuts at: 400 725 923 1702 400
Size: 325 198 779 4388
Fragments arranged by size:
4388 779 325 198
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1885 2014 2325 2581 2659 2866 3745 4237 4535
Size: 129 311 256 78 207 879 492 298
Cuts at: 4535 1885
Size: 3040
Fragments arranged by size:
3040 879 492 311 298 256 207 129
78
BsaI GGTCTCn'nnnn_
Cuts at: 5394 5394
Size: 5690
BsaAI yAC'GTr
Cuts at: 3043 3043
Size: 5690
BsaBI GATnn'nnATC
Cuts at: 6 685 3204 5241 6
Size: 679 2519 2037 455
Fragments arranged by size:
2519 2037 679 455
BsaHI Gr'CG_yC
Cuts at: 2504 3121 3369 4071 5567 2504
Size: 617 248 702 1496 2627
Fragments arranged by size:
2627 1496 702 617 248
BsaWI w'CCGG_w
Cuts at: 2159 2879 3400 4078 4352 4499 4910 2159
Size: 720 521 678 274 147 411 2939
Fragments arranged by size:
2939 720 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1094 1124 1726 1756 1094
Size: 30 602 30 5028
Fragments arranged by size:
5028 602 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 544 1102 1996 2023 544
Size: 558 894 27 4211
Fragments arranged by size:
4211 894 558 27
BseYI C'CCAG_C
Cuts at: 310 350 410 537 548 636 850 900 1030
Size: 40 60 127 11 88 214 50 130
Cuts at: 1030 1196 1235 1273 1502 1656 2634 3115 3873
Size: 166 39 38 229 154 978 481 758
Cuts at: 3873 4450 4946 310
Size: 577 496 1054
Fragments arranged by size:
1054 978 758 577 496 481 229 214
166 154 130 127 88 60 50 40
39 38 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 298 739 1333 283
Size: 15 441 594 4640
Fragments arranged by size:
4640 594 441 15
BsiEI CG_ry'CG
Cuts at: 104 320 3232 3278 4486 104
Size: 216 2912 46 1208 1308
Fragments arranged by size:
2912 1308 1208 216 46
BsiHKAI G_wGCw'C
Cuts at: 617 731 1217 2019 2042 2423 2704 3482 3672
Size: 114 486 802 23 381 281 778 190
Cuts at: 3672 4464 617
Size: 792 1843
Fragments arranged by size:
1843 802 792 778 486 381 281 190
114 23
BsmBI CGTCTCn'nnnn_
Cuts at: 1555 1555
Size: 5690
Bsp1286I G_dGCh'C
Cuts at: 236 567 617 678 731 826 1037 1217 1229
Size: 331 50 61 53 95 211 180 12
Cuts at: 1229 1430 1502 1639 1988 2019 2042 2273 2423
Size: 201 72 137 349 31 23 231 150
Cuts at: 2423 2483 2565 2642 2704 3315 3408 3482 3672
Size: 60 82 77 62 611 93 74 190
Cuts at: 3672 3734 4464 236
Size: 62 730 1462
Fragments arranged by size:
1462 730 611 349 331 231 211 201
190 180 150 137 95 93 82 77
74 72 62 62 61 60 53 50
31 23 12
BspEI T'CCGG_A
Cuts at: 4910 4910
Size: 5690
BspHI T'CATG_A
Cuts at: 4866 4866
Size: 5690
BspMI ACCTGCnnnn'nnnn_
Cuts at: 270 338 928 1145 1355 1436 2740 2954 3256
Size: 68 590 217 210 81 1304 214 302
Cuts at: 3256 3637 270
Size: 381 2323
Fragments arranged by size:
2323 1304 590 381 302 217 214 210
81 68
BsrBI CCG'CTC
Cuts at: 50 322 2522 3982 4036 5520 5685 50
Size: 272 2200 1460 54 1484 165 55
Fragments arranged by size:
2200 1484 1460 272 165 55 54
BsrDI GCAATG_nn'
Cuts at: 489 1073 3602 489
Size: 584 2529 2577
Fragments arranged by size:
2577 2529 584
BsrFI r'CCGG_y
Cuts at: 266 1753 2393 3688 3869 4078 266
Size: 1487 640 1295 181 209 1878
Fragments arranged by size:
1878 1487 1295 640 209 181
BsrGI T'GTAC_A
Cuts at: 248 248
Size: 5690
BssSI C'ACGA_G
Cuts at: 1632 1663 3961 4319 1632
Size: 31 2298 358 3003
Fragments arranged by size:
3003 2298 358 31
BstAPI GCAn_nnn'nTGC
Cuts at: 191 276 297 366 612 2185 2586 3067 191
Size: 85 21 69 246 1573 401 481 2814
Fragments arranged by size:
2814 1573 481 401 246 85 69 21
BstBI TT'CG_AA
Cuts at: 4051 4051
Size: 5690
BstXI CCAn_nnnn'nTGG
Cuts at: 2139 2139
Size: 5690
BstYI r'GATC_y
Cuts at: 1 2651 2715 3182 3205 3540 3786 4798 4884
Size: 2650 64 467 23 335 246 1012 86
Cuts at: 4884 4896 5242 1
Size: 12 346 449
Fragments arranged by size:
2650 1012 467 449 346 335 246 86
64 23 12
Bsu36I CC'TnA_GG
Cuts at: 591 1722 591
Size: 1131 4559
BtgI C'CryG_G
Cuts at: 107 377 698 2096 107
Size: 270 321 1398 3701
Fragments arranged by size:
3701 1398 321 270
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 582 1734 3640 3821 3984 582
Size: 1152 1906 181 163 2288
Fragments arranged by size:
2288 1906 1152 181 163
BtsI GCAGTG_nn'
Cuts at: 150 192 213 1008 1337 1378 1513 1601 1757
Size: 42 21 795 329 41 135 88 156
Cuts at: 1757 2933 150
Size: 1176 2907
Fragments arranged by size:
2907 1176 795 329 156 135 88 42
41 21
DraI TTT'AAA
Cuts at: 2689 2689
Size: 5690
DraIII CAC_nnn'GTG
Cuts at: 2489 2489
Size: 5690
DrdI GACnn_nn'nnGTC
Cuts at: 3396 4254 3396
Size: 858 4832
EaeI y'GGCC_r
Cuts at: 219 317 701 2046 3275 3449 3840 3867 5212
Size: 98 384 1345 1229 174 391 27 1345
Cuts at: 5212 219
Size: 697
Fragments arranged by size:
1345 1345 1229 697 391 384 174 98
27
EagI C'GGCC_G
Cuts at: 317 3275 317
Size: 2958 2732
EarI CTCTTCn'nnn_
Cuts at: 3713 3923 3713
Size: 210 5480
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1870 3898 4208 4354 5500 1870
Size: 2028 310 146 1146 2060
Fragments arranged by size:
2060 2028 1146 310 146
EcoICRI GAG'CTC
Cuts at: 729 2040 2421 2702 729
Size: 1311 381 281 3717
Fragments arranged by size:
3717 1311 381 281
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 400 458 1252 1312 1445 1728 2304 2437
Size: 30 58 794 60 133 283 576 133
Cuts at: 2437 2668 3515 3947 4694 4962 370
Size: 231 847 432 747 268 1098
Fragments arranged by size:
1098 847 794 747 576 432 283 268
231 133 133 60 58 30
EcoO109I rG'GnC_Cy
Cuts at: 233 294 498 823 2412 2442 2638 2829 233
Size: 61 204 325 1589 30 196 191 3094
Fragments arranged by size:
3094 1589 325 204 196 191 61 30
EcoRI G'AATT_C
Cuts at: 2512 2554 2694 2512
Size: 42 140 5508
Fragments arranged by size:
5508 140 42
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3709 3709
Size: 5690
FspI TGC'GCA
Cuts at: 494 3471 494
Size: 2977 2713
HaeII r_GCGC'y
Cuts at: 385 410 2088 2621 3124 3372 4394 5373 385
Size: 25 1678 533 503 248 1022 979 702
Fragments arranged by size:
1678 1022 979 702 533 503 248 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1552 1584 3532 3562 3564 3594 1552
Size: 32 1948 30 2 30 3648
Fragments arranged by size:
3648 1948 32 30 30 2
HincII GTy'rAC
Cuts at: 2729 2729
Size: 5690
KasI G'GCGC_C
Cuts at: 3120 3368 3120
Size: 248 5442
KpnI G_GTAC'C
Cuts at: 2710 2710
Size: 5690
MluI A'CGCG_T
Cuts at: 5123 5123
Size: 5690
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4361 4545 4361
Size: 184 5506
MscI TGG'CCA
Cuts at: 3451 3451
Size: 5690
MslI CAynn'nnrTG
Cuts at: 1329 1606 2434 2668 3679 3806 1329
Size: 277 828 234 1011 127 3213
Fragments arranged by size:
3213 1011 828 277 234 127
MspA1I CmG'CkG
Cuts at: 170 379 808 1095 1465 2409 2801 3115 3475
Size: 209 429 287 370 944 392 314 360
Cuts at: 3475 4488 4733 170
Size: 1013 245 1127
Fragments arranged by size:
1127 1013 944 429 392 370 360 314
287 245 209
NaeI GCC'GGC
Cuts at: 1755 2395 3871 1755
Size: 640 1476 3574
Fragments arranged by size:
3574 1476 640
NarI GG'CG_CC
Cuts at: 3121 3369 3121
Size: 248 5442
NcoI C'CATG_G
Cuts at: 107 2096 107
Size: 1989 3701
NdeI CA'TA_TG
Cuts at: 2435 2435
Size: 5690
NgoMIV G'CCGG_C
Cuts at: 1753 2393 3869 1753
Size: 640 1476 3574
Fragments arranged by size:
3574 1476 640
NsiI A_TGCA'T
Cuts at: 2435 2435
Size: 5690
NspI r_CATG'y
Cuts at: 1825 2433 2673 2743 3243 4150 1825
Size: 608 240 70 500 907 3365
Fragments arranged by size:
3365 907 608 500 240 70
PciI A'CATG_T
Cuts at: 4146 4146
Size: 5690
PflMI CCAn_nnn'nTGG
Cuts at: 423 2489 423
Size: 2066 3624
PfoI T'CCnGG_A
Cuts at: 1844 1844
Size: 5690
PmeI GTTT'AAAC
Cuts at: 2689 2689
Size: 5690
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1720 1752 4829 4861 1720
Size: 32 3077 32 2549
Fragments arranged by size:
3077 2549 32 32
PpuMI rG'GwC_Cy
Cuts at: 2412 2442 2412
Size: 30 5660
PshAI GACnn'nnGTC
Cuts at: 1878 1878
Size: 5690
PspOMI G'GGCC_C
Cuts at: 232 563 822 2638 232
Size: 331 259 1816 3284
Fragments arranged by size:
3284 1816 331 259
PstI C_TGCA'G
Cuts at: 335 374 585 939 1100 1142 1484 2174 2737
Size: 39 211 354 161 42 342 690 563
Cuts at: 2737 3422 335
Size: 685 2603
Fragments arranged by size:
2603 690 685 563 354 342 211 161
42 39
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5690
PvuII CAG'CTG
Cuts at: 1095 1465 3115 3475 1095
Size: 370 1650 360 3310
Fragments arranged by size:
3310 1650 370 360
RsrII CG'GwC_CG
Cuts at: 3885 3885
Size: 5690
SacI G_AGCT'C
Cuts at: 731 2042 2423 2704 731
Size: 1311 381 281 3717
Fragments arranged by size:
3717 1311 381 281
SacII CC_GC'GG
Cuts at: 380 380
Size: 5690
SalI G'TCGA_C
Cuts at: 2727 2727
Size: 5690
SapI GCTCTTCn'nnn_
Cuts at: 3713 3923 3713
Size: 210 5480
SbfI CC_TGCA'GG
Cuts at: 939 2737 939
Size: 1798 3892
ScaI AGT'ACT
Cuts at: 5207 5207
Size: 5690
SexAI A'CCwGG_T
Cuts at: 2444 2444
Size: 5690
SfcI C'TryA_G
Cuts at: 33 331 370 581 935 1096 1138 1480 2170
Size: 298 39 211 354 161 42 342 690
Cuts at: 2170 2733 3418 4411 4602 33
Size: 563 685 993 191 1121
Fragments arranged by size:
1121 993 690 685 563 354 342 298
211 191 161 42 39
SfoI GGC'GCC
Cuts at: 3122 3370 3122
Size: 248 5442
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5690
SgrAI Cr'CCGG_yG
Cuts at: 2393 2393
Size: 5690
SmaI CCC'GGG
Cuts at: 2712 2712
Size: 5690
SmlI C'TyrA_G
Cuts at: 1900 2029 2340 2596 2674 2845 3760 4252 4514
Size: 129 311 256 78 171 915 492 262
Cuts at: 4514 1900
Size: 3076
Fragments arranged by size:
3076 915 492 311 262 256 171 129
78
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5690
SphI G_CATG'C
Cuts at: 2673 2743 3243 2673
Size: 70 500 5120
Fragments arranged by size:
5120 500 70
StyI C'CwwG_G
Cuts at: 107 2096 2237 2824 3178 107
Size: 1989 141 587 354 2619
Fragments arranged by size:
2619 1989 587 354 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1711 2379 3273 3406 3865 4074 1711
Size: 668 894 133 459 209 3327
Fragments arranged by size:
3327 894 668 459 209 133
TatI w'GTAC_w
Cuts at: 248 5205 248
Size: 4957 733
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1094 1126 1907 1939 5207 5239 1094
Size: 32 781 32 3268 32 1545
Fragments arranged by size:
3268 1545 781 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1566 3487 1566
Size: 1921 3769
XbaI T'CTAG_A
Cuts at: 2721 2721
Size: 5690
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3185 3185
Size: 5690
XmaI C'CCGG_G
Cuts at: 2710 2710
Size: 5690
XmnI GAAnn'nnTTC
Cuts at: 471 471
Size: 5690
ZraI GAC'GTC
Cuts at: 2505 2505
Size: 5690
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AloI AscI AvrII BglI BmtI BsiWI
BsmI BssHII BstEII BstZ17I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NheI NotI NruI
PacI PasI PmlI PsiI PspXI PsrI SanDI SfiI
SnaBI SrfI SspI StuI SwaI XhoI