Restriction map for
pF1KB8496
(Circular) (Six-base) MAPSORT of: pF1KB8496.seq Check: 8821 from: 1 to: 5696
pF1KB8496 5696 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 2734 2734
Size: 5696
Acc65I G'GTAC_C
Cuts at: 537 2712 537
Size: 2175 3521
AclI AA'CG_TT
Cuts at: 5536 5536
Size: 5696
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 1127 3521 3953 4700 4968 151
Size: 976 2394 432 747 268 879
Fragments arranged by size:
2394 976 879 747 432 268
AflIII A'CryG_T
Cuts at: 726 2511 3048 4152 5129 726
Size: 1785 537 1104 977 1293
Fragments arranged by size:
1785 1293 1104 977 537
AgeI A'CCGG_T
Cuts at: 4084 4084
Size: 5696
AleI CACnn'nnGTG
Cuts at: 1474 2268 1474
Size: 794 4902
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 678 1616 2286 678
Size: 938 670 4088
Fragments arranged by size:
4088 938 670
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1622 1654 2092 2124 2420 2452 1622
Size: 32 438 32 296 32 4866
Fragments arranged by size:
4866 438 296 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3001 4568 3001
Size: 1567 4129
ApaLI G'TGCA_C
Cuts at: 431 4466 431
Size: 4035 1661
ApoI r'AATT_y
Cuts at: 598 1636 2412 2700 5049 5305 598
Size: 1038 776 288 2349 256 989
Fragments arranged by size:
2349 1038 989 776 288 256
AseI AT'TA_AT
Cuts at: 20 479 20
Size: 459 5237
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5696
AvaI C'yCGr_G
Cuts at: 359 1928 2716 359
Size: 1569 788 3339
Fragments arranged by size:
3339 1569 788
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2527 2560 2527
Size: 33 5663
BamHI G'GATC_C
Cuts at: 2721 2721
Size: 5696
BanI G'GyrC_C
Cuts at: 537 1149 1476 2270 2712 3126 3374 3409 537
Size: 612 327 794 442 414 248 35 2824
Fragments arranged by size:
2824 794 612 442 414 327 248 35
BanII G_rGCy'C
Cuts at: 706 1835 1973 2590 2615 2710 3740 706
Size: 1129 138 617 25 95 1030 2662
Fragments arranged by size:
2662 1129 1030 617 138 95 25
BbeI G_GCGC'C
Cuts at: 3130 3378 3130
Size: 248 5448
BbsI GAAGACnn'nnnn_
Cuts at: 139 568 1732 139
Size: 429 1164 4103
Fragments arranged by size:
4103 1164 429
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1273 1307 1273
Size: 34 5662
BciVI GTATCCnnnnn_n'
Cuts at: 1252 3594 4355 5696 1252
Size: 2342 761 1341 1252
Fragments arranged by size:
2342 1341 1252 761
BclI T'GATC_A
Cuts at: 3216 3216
Size: 5696
BglII A'GATC_T
Cuts at: 937 1138 2300 3211 5248 937
Size: 201 1162 911 2037 1385
Fragments arranged by size:
2037 1385 1162 911 201
BlpI GC'TnA_GC
Cuts at: 2808 2808
Size: 5696
Bme1580I G_kGCm'C
Cuts at: 435 3321 3414 4470 435
Size: 2886 93 1056 1661
Fragments arranged by size:
2886 1661 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2128 2908 2989 3324 5468 2128
Size: 780 81 335 2144 2356
Fragments arranged by size:
2356 2144 780 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 939 2467 4063 939
Size: 1528 1596 2572
Fragments arranged by size:
2572 1596 1528
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 838 866 953 1591 2276 2497 838
Size: 28 87 638 685 221 4037
Fragments arranged by size:
4037 685 638 221 87 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 1477 2173 2872 3751 4243 4541 334
Size: 1143 696 699 879 492 298 1489
Fragments arranged by size:
1489 1143 879 699 696 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 481 2554 5400 481
Size: 2073 2846 777
Fragments arranged by size:
2846 2073 777
BsaAI yAC'GTr
Cuts at: 3049 3049
Size: 5696
BsaBI GATnn'nnATC
Cuts at: 6 244 3210 5247 6
Size: 238 2966 2037 455
Fragments arranged by size:
2966 2037 455 238
BsaHI Gr'CG_yC
Cuts at: 3127 3375 4077 5573 3127
Size: 248 702 1496 3250
Fragments arranged by size:
3250 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1684 1705 2885 3406 4084 4358 4505 4916 1684
Size: 21 1180 521 678 274 147 411 2464
Fragments arranged by size:
2464 1180 678 521 411 274 147 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 745 775 951 981 1215 1245 2572 2602 745
Size: 30 176 30 234 30 1327 30 3839
Fragments arranged by size:
3839 1327 234 176 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 332 1117 1511 332
Size: 785 394 4517
Fragments arranged by size:
4517 785 394
BseYI C'CCAG_C
Cuts at: 717 851 2315 2614 3121 3879 4456 4952 717
Size: 134 1464 299 507 758 577 496 1461
Fragments arranged by size:
1464 1461 758 577 507 496 299 134
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 674 716 1908 2544 169
Size: 505 42 1192 636 3321
Fragments arranged by size:
3321 1192 636 505 42
BsiEI CG_ry'CG
Cuts at: 104 3238 3284 4492 104
Size: 3134 46 1208 1308
Fragments arranged by size:
3134 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 360 435 1413 1973 2710 3488 3678 4470 360
Size: 75 978 560 737 778 190 792 1586
Fragments arranged by size:
1586 978 792 778 737 560 190 75
Bsp1286I G_dGCh'C
Cuts at: 360 435 706 1413 1835 1973 2590 2615 2710
Size: 75 271 707 422 138 617 25 95
Cuts at: 2710 3321 3414 3488 3678 3740 4470 360
Size: 611 93 74 190 62 730 1586
Fragments arranged by size:
1586 730 707 617 611 422 271 190
138 95 93 75 74 62 25
BspEI T'CCGG_A
Cuts at: 4916 4916
Size: 5696
BspHI T'CATG_A
Cuts at: 1740 2093 4872 1740
Size: 353 2779 2564
Fragments arranged by size:
2779 2564 353
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1442 2087 2746 2960 3262 3643 1442
Size: 645 659 214 302 381 3495
Fragments arranged by size:
3495 659 645 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1519 3988 4042 5526 5691 50
Size: 1469 2469 54 1484 165 55
Fragments arranged by size:
2469 1484 1469 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3608 3608
Size: 5696
BsrFI r'CCGG_y
Cuts at: 3694 3875 4084 3694
Size: 181 209 5306
Fragments arranged by size:
5306 209 181
BsrGI T'GTAC_A
Cuts at: 1697 2006 1697
Size: 309 5387
BssSI C'ACGA_G
Cuts at: 3967 4325 3967
Size: 358 5338
BstAPI GCAn_nnn'nTGC
Cuts at: 1857 3073 1857
Size: 1216 4480
BstBI TT'CG_AA
Cuts at: 323 1081 4057 323
Size: 758 2976 1962
Fragments arranged by size:
2976 1962 758
BstEII G'GTnAC_C
Cuts at: 835 1934 2343 835
Size: 1099 409 4188
Fragments arranged by size:
4188 1099 409
BstXI CCAn_nnnn'nTGG
Cuts at: 1569 2073 2118 2226 1569
Size: 504 45 108 5039
Fragments arranged by size:
5039 504 108 45
BstYI r'GATC_y
Cuts at: 1 937 1138 1526 2171 2300 2721 3188 3211
Size: 936 201 388 645 129 421 467 23
Cuts at: 3211 3546 3792 4804 4890 4902 5248 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 936 645 467 449 421 388 346
335 246 201 129 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1940 2176 1940
Size: 236 5460
BtgI C'CryG_G
Cuts at: 107 2564 107
Size: 2457 3239
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1033 3646 3827 3990 1033
Size: 2613 181 163 2739
Fragments arranged by size:
2739 2613 181 163
BtsI GCAGTG_nn'
Cuts at: 1374 2939 1374
Size: 1565 4131
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 515 550 515
Size: 35 5661
DraI TTT'AAA
Cuts at: 2695 2695
Size: 5696
DraIII CAC_nnn'GTG
Cuts at: 1513 1513
Size: 5696
DrdI GACnn_nn'nnGTC
Cuts at: 3402 4260 3402
Size: 858 4838
EaeI y'GGCC_r
Cuts at: 731 2402 2567 3281 3455 3846 3873 5218 731
Size: 1671 165 714 174 391 27 1345 1209
Fragments arranged by size:
1671 1345 1209 714 391 174 165 27
EagI C'GGCC_G
Cuts at: 3281 3281
Size: 5696
EarI CTCTTCn'nnn_
Cuts at: 1319 1373 1480 1622 3719 3929 1319
Size: 54 107 142 2097 210 3086
Fragments arranged by size:
3086 2097 210 142 107 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 747 3904 4214 4360 5506 747
Size: 3157 310 146 1146 937
Fragments arranged by size:
3157 1146 937 310 146
EcoICRI GAG'CTC
Cuts at: 1971 2708 1971
Size: 737 4959
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 838 866 953 1127 1591 2276 2497 3521
Size: 687 28 87 174 464 685 221 1024
Cuts at: 3521 3953 4700 4968 151
Size: 432 747 268 879
Fragments arranged by size:
1024 879 747 687 685 464 432 268
221 174 87 28
EcoNI CCTnn'n_nnAGG
Cuts at: 1471 1718 1762 1471
Size: 247 44 5405
Fragments arranged by size:
5405 247 44
EcoO109I rG'GnC_Cy
Cuts at: 164 2180 2835 164
Size: 2016 655 3025
Fragments arranged by size:
3025 2016 655
EcoRI G'AATT_C
Cuts at: 1636 2700 1636
Size: 1064 4632
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 585 1473 3715 585
Size: 888 2242 2566
Fragments arranged by size:
2566 2242 888
FspI TGC'GCA
Cuts at: 3477 3477
Size: 5696
HaeII r_GCGC'y
Cuts at: 3130 3378 4400 5379 3130
Size: 248 1022 979 3447
Fragments arranged by size:
3447 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1215 1247 1257 1289 1518 1550 2490 2522 3538
Size: 32 10 32 229 32 940 32 1016
Cuts at: 3538 3568 3570 3600 1215
Size: 30 2 30 3311
Fragments arranged by size:
3311 1016 940 229 32 32 32 32
30 30 10 2
HincII GTy'rAC
Cuts at: 2509 2735 2509
Size: 226 5470
KasI G'GCGC_C
Cuts at: 3126 3374 3126
Size: 248 5448
KpnI G_GTAC'C
Cuts at: 541 2716 541
Size: 2175 3521
MfeI C'AATT_G
Cuts at: 2060 2060
Size: 5696
MluI A'CGCG_T
Cuts at: 5129 5129
Size: 5696
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 152 4367 4551 152
Size: 4215 184 1297
Fragments arranged by size:
4215 1297 184
MscI TGG'CCA
Cuts at: 733 3457 733
Size: 2724 2972
MslI CAynn'nnrTG
Cuts at: 1474 2268 2569 3685 3812 1474
Size: 794 301 1116 127 3358
Fragments arranged by size:
3358 1116 794 301 127
MspA1I CmG'CkG
Cuts at: 2163 2226 2807 3121 3481 4494 4739 2163
Size: 63 581 314 360 1013 245 3120
Fragments arranged by size:
3120 1013 581 360 314 245 63
NaeI GCC'GGC
Cuts at: 3877 3877
Size: 5696
NarI GG'CG_CC
Cuts at: 3127 3375 3127
Size: 248 5448
NcoI C'CATG_G
Cuts at: 107 2564 107
Size: 2457 3239
NgoMIV G'CCGG_C
Cuts at: 3875 3875
Size: 5696
NspI r_CATG'y
Cuts at: 730 1597 1698 2515 2749 3249 4156 730
Size: 867 101 817 234 500 907 2270
Fragments arranged by size:
2270 907 867 817 500 234 101
PasI CC'CwG_GG
Cuts at: 1714 1714
Size: 5696
PciI A'CATG_T
Cuts at: 726 2511 4152 726
Size: 1785 1641 2270
Fragments arranged by size:
2270 1785 1641
PflMI CCAn_nnn'nTGG
Cuts at: 2361 2361
Size: 5696
PfoI T'CCnGG_A
Cuts at: 583 882 1426 583
Size: 299 544 4853
Fragments arranged by size:
4853 544 299
PmeI GTTT'AAAC
Cuts at: 2695 2695
Size: 5696
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4835 4867 4835
Size: 32 5664
PpuMI rG'GwC_Cy
Cuts at: 2180 2180
Size: 5696
PspXI vC'TCGA_Gb
Cuts at: 359 359
Size: 5696
PstI C_TGCA'G
Cuts at: 1763 2168 2287 2743 3428 1763
Size: 405 119 456 685 4031
Fragments arranged by size:
4031 685 456 405 119
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5696
PvuII CAG'CTG
Cuts at: 2163 2226 3121 3481 2163
Size: 63 895 360 4378
Fragments arranged by size:
4378 895 360 63
RsrII CG'GwC_CG
Cuts at: 3891 3891
Size: 5696
SacI G_AGCT'C
Cuts at: 1973 2710 1973
Size: 737 4959
SalI G'TCGA_C
Cuts at: 2733 2733
Size: 5696
SapI GCTCTTCn'nnn_
Cuts at: 3719 3929 3719
Size: 210 5486
SbfI CC_TGCA'GG
Cuts at: 2743 2743
Size: 5696
ScaI AGT'ACT
Cuts at: 5213 5213
Size: 5696
SfcI C'TryA_G
Cuts at: 33 198 1759 2164 2283 2739 3424 4417 4608
Size: 165 1561 405 119 456 685 993 191
Cuts at: 4608 33
Size: 1121
Fragments arranged by size:
1561 1121 993 685 456 405 191 165
119
SfoI GGC'GCC
Cuts at: 3128 3376 3128
Size: 248 5448
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5696
SmaI CCC'GGG
Cuts at: 2718 2718
Size: 5696
SmlI C'TyrA_G
Cuts at: 313 359 1492 2188 2851 3766 4258 4520 313
Size: 46 1133 696 663 915 492 262 1489
Fragments arranged by size:
1489 1133 915 696 663 492 262 46
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5696
SphI G_CATG'C
Cuts at: 2749 3249 2749
Size: 500 5196
SspI AAT'ATT
Cuts at: 443 443
Size: 5696
StuI AGG'CCT
Cuts at: 2203 2203
Size: 5696
StyI C'CwwG_G
Cuts at: 107 872 1402 2564 2830 3184 107
Size: 765 530 1162 266 354 2619
Fragments arranged by size:
2619 1162 765 530 354 266
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1428 3279 3412 3871 4080 1428
Size: 1851 133 459 209 3044
Fragments arranged by size:
3044 1851 459 209 133
TatI w'GTAC_w
Cuts at: 363 1697 2006 5211 363
Size: 1334 309 3205 848
Fragments arranged by size:
3205 1334 848 309
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 248 280 414 446 745 777 2572 2604 5213
Size: 32 134 32 299 32 1795 32 2609
Cuts at: 5213 5245 248
Size: 32 699
Fragments arranged by size:
2609 1795 699 299 134 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2120 3493 2120
Size: 1373 4323
XbaI T'CTAG_A
Cuts at: 2727 2727
Size: 5696
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 813 3191 813
Size: 2378 3318
XhoI C'TCGA_G
Cuts at: 359 359
Size: 5696
XmaI C'CCGG_G
Cuts at: 2716 2716
Size: 5696
XmnI GAAnn'nnTTC
Cuts at: 598 1254 598
Size: 656 5040
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI ApaI AscI AvrII
BbvCI BfrBI BglI BmgBI BmtI Bpu10I BsiWI BsmI
BsmBI BssHII BstZ17I ClaI EcoRV FseI FspAI HindIII
HpaI NdeI NheI NotI NruI NsiI PacI PmlI
PshAI PsiI PspOMI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SwaI ZraI