Restriction map for
pF1KB8499
(Circular) (Six-base) MAPSORT of: pF1KB8499.seq Check: 1750 from: 1 to: 5909
pF1KB8499 5909 bp
With 182 enzymes: SgfI *
September 25, 2012 15:38 ..
AccI GT'mk_AC
Cuts at: 900 2712 2947 900
Size: 1812 235 3862
Fragments arranged by size:
3862 1812 235
Acc65I G'GTAC_C
Cuts at: 2925 2925
Size: 5909
AclI AA'CG_TT
Cuts at: 5749 5749
Size: 5909
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 815 1247 2137 3734 4166 4913 5181 815
Size: 432 890 1597 432 747 268 1543
Fragments arranged by size:
1597 1543 890 747 432 432 268
AflII C'TTAA_G
Cuts at: 663 663
Size: 5909
AflIII A'CryG_T
Cuts at: 3261 4365 5342 3261
Size: 1104 977 3828
Fragments arranged by size:
3828 1104 977
AgeI A'CCGG_T
Cuts at: 4297 4297
Size: 5909
AleI CACnn'nnGTG
Cuts at: 166 166
Size: 5909
AlwNI CAG_nnn'CTG
Cuts at: 3214 4781 3214
Size: 1567 4342
ApaLI G'TGCA_C
Cuts at: 4679 4679
Size: 5909
ApoI r'AATT_y
Cuts at: 363 1032 1137 1379 1583 1623 1655 2088 2232
Size: 669 105 242 204 40 32 433 144
Cuts at: 2232 2663 2913 5262 5518 363
Size: 431 250 2349 256 754
Fragments arranged by size:
2349 754 669 433 431 256 250 242
204 144 105 40 32
AseI AT'TA_AT
Cuts at: 20 20
Size: 5909
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5909
AvaI C'yCGr_G
Cuts at: 2929 2929
Size: 5909
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 518 551 1250 1283 1989 2022 518
Size: 33 699 33 706 33 4405
Fragments arranged by size:
4405 706 699 33 33 33
BamHI G'GATC_C
Cuts at: 2934 2934
Size: 5909
BanI G'GyrC_C
Cuts at: 214 2925 3339 3587 3622 214
Size: 2711 414 248 35 2501
Fragments arranged by size:
2711 2501 414 248 35
BanII G_rGCy'C
Cuts at: 2923 3953 2923
Size: 1030 4879
BbeI G_GCGC'C
Cuts at: 3343 3591 3343
Size: 248 5661
BbsI GAAGACnn'nnnn_
Cuts at: 1942 1942
Size: 5909
BbvCI CC'TCA_GC
Cuts at: 419 419
Size: 5909
BciVI GTATCCnnnnn_n'
Cuts at: 658 992 3807 4568 5909 658
Size: 334 2815 761 1341 658
Fragments arranged by size:
2815 1341 761 658 334
BclI T'GATC_A
Cuts at: 3429 3429
Size: 5909
BfrBI ATG'CAT
Cuts at: 1290 1411 2311 1290
Size: 121 900 4888
Fragments arranged by size:
4888 900 121
BglII A'GATC_T
Cuts at: 354 627 3424 5461 354
Size: 273 2797 2037 802
Fragments arranged by size:
2797 2037 802 273
BlpI GC'TnA_GC
Cuts at: 3021 3021
Size: 5909
Bme1580I G_kGCm'C
Cuts at: 3534 3627 4683 3534
Size: 93 1056 4760
Fragments arranged by size:
4760 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 459 1497 3121 3202 3537 5681 459
Size: 1038 1624 81 335 2144 687
Fragments arranged by size:
2144 1624 1038 687 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4276 4276
Size: 5909
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 259
Size: 5909
Bpu10I CC'TnA_GC
Cuts at: 419 419
Size: 5909
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2413 3085 3964 4456 4754 2413
Size: 672 879 492 298 3568
Fragments arranged by size:
3568 879 672 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5613 5613
Size: 5909
BsaAI yAC'GTr
Cuts at: 3262 3262
Size: 5909
BsaBI GATnn'nnATC
Cuts at: 6 3423 5460 6
Size: 3417 2037 455
Fragments arranged by size:
3417 2037 455
BsaHI Gr'CG_yC
Cuts at: 3340 3588 4290 5786 3340
Size: 248 702 1496 3463
Fragments arranged by size:
3463 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3098 3619 4297 4571 4718 5129 3098
Size: 521 678 274 147 411 3878
Fragments arranged by size:
3878 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2735 2765 2735
Size: 30 5879
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 622 622
Size: 5909
BseYI C'CCAG_C
Cuts at: 3334 4092 4669 5165 3334
Size: 758 577 496 4078
Fragments arranged by size:
4078 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 3451 3497 4705 104
Size: 3347 46 1208 1308
Fragments arranged by size:
3347 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1845 2923 3701 3891 4683 1845
Size: 1078 778 190 792 3071
Fragments arranged by size:
3071 1078 792 778 190
BsmI GAATG_Cn'
Cuts at: 1413 1413
Size: 5909
Bsp1286I G_dGCh'C
Cuts at: 1845 2923 3534 3627 3701 3891 3953 4683 1845
Size: 1078 611 93 74 190 62 730 3071
Fragments arranged by size:
3071 1078 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 5129 5129
Size: 5909
BspHI T'CATG_A
Cuts at: 348 635 5085 348
Size: 287 4450 1172
Fragments arranged by size:
4450 1172 287
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1552 2959 3173 3475 3856 1552
Size: 1407 214 302 381 3605
Fragments arranged by size:
3605 1407 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4201 4255 5739 5904 50
Size: 4151 54 1484 165 55
Fragments arranged by size:
4151 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 323 3821 323
Size: 3498 2411
BsrFI r'CCGG_y
Cuts at: 3907 4088 4297 3907
Size: 181 209 5519
Fragments arranged by size:
5519 209 181
BsrGI T'GTAC_A
Cuts at: 678 678
Size: 5909
BssSI C'ACGA_G
Cuts at: 4180 4538 4180
Size: 358 5551
BstAPI GCAn_nnn'nTGC
Cuts at: 3286 3286
Size: 5909
BstBI TT'CG_AA
Cuts at: 4270 4270
Size: 5909
BstYI r'GATC_y
Cuts at: 1 354 620 627 2201 2461 2934 3401 3424
Size: 353 266 7 1574 260 473 467 23
Cuts at: 3424 3759 4005 5017 5103 5115 5461 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1574 1012 473 467 449 353 346 335
266 260 246 86 23 12 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3859 4040 4203 3859
Size: 181 163 5565
Fragments arranged by size:
5565 181 163
BtsI GCAGTG_nn'
Cuts at: 489 2756 3152 489
Size: 2267 396 3246
Fragments arranged by size:
3246 2267 396
ClaI AT'CG_AT
Cuts at: 1004 1004
Size: 5909
DraI TTT'AAA
Cuts at: 1328 2382 2657 2908 1328
Size: 1054 275 251 4329
Fragments arranged by size:
4329 1054 275 251
DrdI GACnn_nn'nnGTC
Cuts at: 3615 4473 3615
Size: 858 5051
EaeI y'GGCC_r
Cuts at: 486 3494 3668 4059 4086 5431 486
Size: 3008 174 391 27 1345 964
Fragments arranged by size:
3008 1345 964 391 174 27
EagI C'GGCC_G
Cuts at: 3494 3494
Size: 5909
EarI CTCTTCn'nnn_
Cuts at: 1581 1943 1964 2008 2258 3932 4142 1581
Size: 362 21 44 250 1674 210 3348
Fragments arranged by size:
3348 1674 362 250 210 44 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4117 4427 4573 5719 4117
Size: 310 146 1146 4307
Fragments arranged by size:
4307 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2921 2921
Size: 5909
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 815 1247 2137 3734 4166 4913 5181 259
Size: 556 432 890 1597 432 747 268 987
Fragments arranged by size:
1597 987 890 747 556 432 432 268
EcoO109I rG'GnC_Cy
Cuts at: 3048 3048
Size: 5909
EcoRI G'AATT_C
Cuts at: 2913 2913
Size: 5909
EcoRV GAT'ATC
Cuts at: 921 921
Size: 5909
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1582 2683 3928 1582
Size: 1101 1245 3563
Fragments arranged by size:
3563 1245 1101
FspI TGC'GCA
Cuts at: 3690 3690
Size: 5909
HaeII r_GCGC'y
Cuts at: 3343 3591 4613 5592 3343
Size: 248 1022 979 3660
Fragments arranged by size:
3660 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 612 644 1759 1791 2277 2309 3751 3781 3783
Size: 32 1115 32 486 32 1442 30 2
Cuts at: 3783 3813 612
Size: 30 2708
Fragments arranged by size:
2708 1442 1115 486 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 901 2256 2441 2948 901
Size: 1355 185 507 3862
Fragments arranged by size:
3862 1355 507 185
HpaI GTT'AAC
Cuts at: 2441 2441
Size: 5909
KasI G'GCGC_C
Cuts at: 3339 3587 3339
Size: 248 5661
KpnI G_GTAC'C
Cuts at: 2929 2929
Size: 5909
MfeI C'AATT_G
Cuts at: 115 115
Size: 5909
MluI A'CGCG_T
Cuts at: 5342 5342
Size: 5909
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 960 4580 4764 960
Size: 3620 184 2105
Fragments arranged by size:
3620 2105 184
MscI TGG'CCA
Cuts at: 488 3670 488
Size: 3182 2727
MslI CAynn'nnrTG
Cuts at: 166 1295 1426 1532 3898 4025 166
Size: 1129 131 106 2366 127 2050
Fragments arranged by size:
2366 2050 1129 131 127 106
MspA1I CmG'CkG
Cuts at: 1018 1174 3020 3334 3694 4707 4952 1018
Size: 156 1846 314 360 1013 245 1975
Fragments arranged by size:
1975 1846 1013 360 314 245 156
NaeI GCC'GGC
Cuts at: 4090 4090
Size: 5909
NarI GG'CG_CC
Cuts at: 3340 3588 3340
Size: 248 5661
NgoMIV G'CCGG_C
Cuts at: 4088 4088
Size: 5909
NsiI A_TGCA'T
Cuts at: 1292 1413 2313 1292
Size: 121 900 4888
Fragments arranged by size:
4888 900 121
NspI r_CATG'y
Cuts at: 2962 3462 4369 2962
Size: 500 907 4502
Fragments arranged by size:
4502 907 500
PciI A'CATG_T
Cuts at: 4365 4365
Size: 5909
PflMI CCAn_nnn'nTGG
Cuts at: 2084 2641 2084
Size: 557 5352
PmeI GTTT'AAAC
Cuts at: 2908 2908
Size: 5909
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5048 5080 5048
Size: 32 5877
PsiI TTA'TAA
Cuts at: 1483 1483
Size: 5909
PstI C_TGCA'G
Cuts at: 1593 2415 2956 3641 1593
Size: 822 541 685 3861
Fragments arranged by size:
3861 822 685 541
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5909
PvuII CAG'CTG
Cuts at: 1018 1174 3334 3694 1018
Size: 156 2160 360 3233
Fragments arranged by size:
3233 2160 360 156
RsrII CG'GwC_CG
Cuts at: 4104 4104
Size: 5909
SacI G_AGCT'C
Cuts at: 2923 2923
Size: 5909
SalI G'TCGA_C
Cuts at: 899 2946 899
Size: 2047 3862
SapI GCTCTTCn'nnn_
Cuts at: 3932 4142 3932
Size: 210 5699
SbfI CC_TGCA'GG
Cuts at: 2956 2956
Size: 5909
ScaI AGT'ACT
Cuts at: 325 5426 325
Size: 5101 808
SfcI C'TryA_G
Cuts at: 33 1013 1589 2411 2811 2900 2952 3637 4630
Size: 980 576 822 400 89 52 685 993
Cuts at: 4630 4821 33
Size: 191 1121
Fragments arranged by size:
1121 993 980 822 685 576 400 191
89 52
SfoI GGC'GCC
Cuts at: 3341 3589 3341
Size: 248 5661
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5909
SmaI CCC'GGG
Cuts at: 2931 2931
Size: 5909
SmlI C'TyrA_G
Cuts at: 663 2392 3064 3979 4471 4733 663
Size: 1729 672 915 492 262 1839
Fragments arranged by size:
1839 1729 915 672 492 262
SpeI A'CTAG_T
Cuts at: 67 856 1894 67
Size: 789 1038 4082
Fragments arranged by size:
4082 1038 789
SphI G_CATG'C
Cuts at: 2962 3462 2962
Size: 500 5409
SspI AAT'ATT
Cuts at: 1641 2187 1641
Size: 546 5363
StyI C'CwwG_G
Cuts at: 203 395 3043 3397 203
Size: 192 2648 354 2715
Fragments arranged by size:
2715 2648 354 192
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 478 1516 3492 3625 4084 4293 478
Size: 1038 1976 133 459 209 2094
Fragments arranged by size:
2094 1976 1038 459 209 133
TatI w'GTAC_w
Cuts at: 323 678 906 1132 2777 5424 323
Size: 355 228 226 1645 2647 808
Fragments arranged by size:
2647 1645 808 355 228 226
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2735 2767 5426 5458 2735
Size: 32 2659 32 3186
Fragments arranged by size:
3186 2659 32 32
Tth111I GACn'n_nGTC
Cuts at: 3706 3706
Size: 5909
XbaI T'CTAG_A
Cuts at: 2940 2940
Size: 5909
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1342 1510 2512 3404 1342
Size: 168 1002 892 3847
Fragments arranged by size:
3847 1002 892 168
XmaI C'CCGG_G
Cuts at: 2929 2929
Size: 5909
XmnI GAAnn'nnTTC
Cuts at: 779 2150 2557 779
Size: 1371 407 4131
Fragments arranged by size:
4131 1371 407
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PciI PflMI PmeI PpiI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI ApaI AscI
AvrII BcgI BglI BmgBI BmtI BsgI BsiWI BsmBI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI CspCI DraIII
EcoNI FseI FspAI HindIII NcoI NdeI NheI NotI
NruI PacI PasI PfoI PmlI PpuMI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI