Restriction map for
pF1KB8500
(Circular) (Six-base) MAPSORT of: pF1KB8500.seq Check: 4456 from: 1 to: 5699
pF1KB8500 5699 bp
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 186 186
Size: 5699
AatII G_ACGT'C
Cuts at: 2456 2456
Size: 5699
AccI GT'mk_AC
Cuts at: 1034 2737 1034
Size: 1703 3996
Acc65I G'GTAC_C
Cuts at: 2715 2715
Size: 5699
AclI AA'CG_TT
Cuts at: 2693 5539 2693
Size: 2846 2853
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1466 3524 3956 4703 4971 1466
Size: 2058 432 747 268 2194
Fragments arranged by size:
2194 2058 747 432 268
AflII C'TTAA_G
Cuts at: 280 280
Size: 5699
AflIII A'CryG_T
Cuts at: 1040 1349 1701 1904 3051 4155 5132 1040
Size: 309 352 203 1147 1104 977 1607
Fragments arranged by size:
1607 1147 1104 977 352 309 203
AgeI A'CCGG_T
Cuts at: 4087 4087
Size: 5699
AleI CACnn'nnGTG
Cuts at: 1606 1606
Size: 5699
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1029 2543 1029
Size: 1514 4185
AlwNI CAG_nnn'CTG
Cuts at: 139 1236 1649 2392 2540 3004 4571 139
Size: 1097 413 743 148 464 1567 1267
Fragments arranged by size:
1567 1267 1097 743 464 413 148
ApaLI G'TGCA_C
Cuts at: 452 4469 452
Size: 4017 1682
ApoI r'AATT_y
Cuts at: 622 1133 1789 1856 2703 5052 5308 622
Size: 511 656 67 847 2349 256 1013
Fragments arranged by size:
2349 1013 847 656 511 256 67
AseI AT'TA_AT
Cuts at: 20 1940 20
Size: 1920 3779
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5699
AvaI C'yCGr_G
Cuts at: 126 1587 2510 2719 126
Size: 1461 923 209 3106
Fragments arranged by size:
3106 1461 923 209
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1042 1075 1042
Size: 33 5666
BamHI G'GATC_C
Cuts at: 2724 2724
Size: 5699
BanI G'GyrC_C
Cuts at: 256 1486 2715 3129 3377 3412 256
Size: 1230 1229 414 248 35 2543
Fragments arranged by size:
2543 1230 1229 414 248 35
BanII G_rGCy'C
Cuts at: 127 445 1444 1693 2713 3743 127
Size: 318 999 249 1020 1030 2083
Fragments arranged by size:
2083 1030 1020 999 318 249
BbeI G_GCGC'C
Cuts at: 3133 3381 3133
Size: 248 5451
BbsI GAAGACnn'nnnn_
Cuts at: 1463 1463
Size: 5699
BbvCI CC'TCA_GC
Cuts at: 1805 1805
Size: 5699
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2362 2396 2362
Size: 34 5665
BciVI GTATCCnnnnn_n'
Cuts at: 1070 3597 4358 5699 1070
Size: 2527 761 1341 1070
Fragments arranged by size:
2527 1341 1070 761
BclI T'GATC_A
Cuts at: 519 2597 3219 519
Size: 2078 622 2999
Fragments arranged by size:
2999 2078 622
BfrBI ATG'CAT
Cuts at: 676 1269 676
Size: 593 5106
BglII A'GATC_T
Cuts at: 1513 3214 5251 1513
Size: 1701 2037 1961
Fragments arranged by size:
2037 1961 1701
BlpI GC'TnA_GC
Cuts at: 2476 2811 2476
Size: 335 5364
Bme1580I G_kGCm'C
Cuts at: 456 1595 1958 3324 3417 4473 456
Size: 1139 363 1366 93 1056 1682
Fragments arranged by size:
1682 1366 1139 1056 363 93
BmgBI CAC'GTC
Cuts at: 1350 1350
Size: 5699
BmrI ACTGGGnnnn_n'
Cuts at: 1544 2911 2992 3327 5471 1544
Size: 1367 81 335 2144 1772
Fragments arranged by size:
2144 1772 1367 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1602 4066 1602
Size: 2464 3235
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1103 1352 1409 2090 2245 2369 2517 1103
Size: 249 57 681 155 124 148 4285
Fragments arranged by size:
4285 681 249 155 148 124 57
Bpu10I CC'TnA_GC
Cuts at: 143 1805 143
Size: 1662 4037
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2875 3754 4246 4544 2875
Size: 879 492 298 4030
Fragments arranged by size:
4030 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5403 5403
Size: 5699
BsaAI yAC'GTr
Cuts at: 3052 3052
Size: 5699
BsaBI GATnn'nnATC
Cuts at: 6 3213 5250 6
Size: 3207 2037 455
Fragments arranged by size:
3207 2037 455
BsaHI Gr'CG_yC
Cuts at: 1505 2453 3130 3378 4080 5576 1505
Size: 948 677 248 702 1496 1628
Fragments arranged by size:
1628 1496 948 702 677 248
BsaWI w'CCGG_w
Cuts at: 1780 2888 3409 4087 4361 4508 4919 1780
Size: 1108 521 678 274 147 411 2560
Fragments arranged by size:
2560 1108 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 769 1002 3124 3882 4459 4955 769
Size: 233 2122 758 577 496 1513
Fragments arranged by size:
2122 1513 758 577 496 233
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 604
Size: 5699
BsiEI CG_ry'CG
Cuts at: 104 3241 3287 4495 104
Size: 3137 46 1208 1308
Fragments arranged by size:
3137 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 127 445 456 2261 2713 3491 3681 4473 127
Size: 318 11 1805 452 778 190 792 1353
Fragments arranged by size:
1805 1353 792 778 452 318 190 11
BsmI GAATG_Cn'
Cuts at: 1102 1102
Size: 5699
Bsp1286I G_dGCh'C
Cuts at: 127 445 456 1444 1595 1693 1958 2261 2713
Size: 318 11 988 151 98 265 303 452
Cuts at: 2713 3324 3417 3491 3681 3743 4473 127
Size: 611 93 74 190 62 730 1353
Fragments arranged by size:
1353 988 730 611 452 318 303 265
190 151 98 93 74 62 11
BspEI T'CCGG_A
Cuts at: 4919 4919
Size: 5699
BspHI T'CATG_A
Cuts at: 2007 4875 2007
Size: 2868 2831
BspMI ACCTGCnnnn'nnnn_
Cuts at: 147 186 487 2749 2963 3265 3646 147
Size: 39 301 2262 214 302 381 2200
Fragments arranged by size:
2262 2200 381 302 301 214 39
BsrBI CCG'CTC
Cuts at: 50 1570 3991 4045 5529 5694 50
Size: 1520 2421 54 1484 165 55
Fragments arranged by size:
2421 1520 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3611 3611
Size: 5699
BsrFI r'CCGG_y
Cuts at: 986 2501 3697 3878 4087 986
Size: 1515 1196 181 209 2598
Fragments arranged by size:
2598 1515 1196 209 181
BssSI C'ACGA_G
Cuts at: 1021 3970 4328 1021
Size: 2949 358 2392
Fragments arranged by size:
2949 2392 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1137 1236 1649 2256 3076 1137
Size: 99 413 607 820 3760
Fragments arranged by size:
3760 820 607 413 99
BstBI TT'CG_AA
Cuts at: 347 4060 347
Size: 3713 1986
BstYI r'GATC_y
Cuts at: 1 297 1513 2724 3191 3214 3549 3795 4807
Size: 296 1216 1211 467 23 335 246 1012
Cuts at: 4807 4893 4905 5251 1
Size: 86 12 346 449
Fragments arranged by size:
1216 1211 1012 467 449 346 335 296
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 1292 1607 2213 2640 107
Size: 1185 315 606 427 3166
Fragments arranged by size:
3166 1185 606 427 315
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 476 2649 3649 3830 3993 476
Size: 2173 1000 181 163 2182
Fragments arranged by size:
2182 2173 1000 181 163
BtsI GCAGTG_nn'
Cuts at: 215 1234 1535 1647 2942 215
Size: 1019 301 112 1295 2972
Fragments arranged by size:
2972 1295 1019 301 112
ClaI AT'CG_AT
Cuts at: 2372 2372
Size: 5699
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 249 284 306 341 249
Size: 35 22 35 5607
Fragments arranged by size:
5607 35 35 22
DraI TTT'AAA
Cuts at: 1172 1845 2698 1172
Size: 673 853 4173
Fragments arranged by size:
4173 853 673
DraIII CAC_nnn'GTG
Cuts at: 451 2256 451
Size: 1805 3894
DrdI GACnn_nn'nnGTC
Cuts at: 1504 3405 4263 1504
Size: 1901 858 2940
Fragments arranged by size:
2940 1901 858
EaeI y'GGCC_r
Cuts at: 1667 3284 3458 3849 3876 5221 1667
Size: 1617 174 391 27 1345 2145
Fragments arranged by size:
2145 1617 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3284 3284
Size: 5699
EarI CTCTTCn'nnn_
Cuts at: 860 1388 3722 3932 860
Size: 528 2334 210 2627
Fragments arranged by size:
2627 2334 528 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3907 4217 4363 5509 3907
Size: 310 146 1146 4097
Fragments arranged by size:
4097 1146 310 146
EcoICRI GAG'CTC
Cuts at: 125 443 2711 125
Size: 318 2268 3113
Fragments arranged by size:
3113 2268 318
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1103 1352 1409 1466 2090 2245 2369 2517 3524
Size: 249 57 57 624 155 124 148 1007
Cuts at: 3524 3956 4703 4971 1103
Size: 432 747 268 1831
Fragments arranged by size:
1831 1007 747 624 432 268 249 155
148 124 57 57
EcoNI CCTnn'n_nnAGG
Cuts at: 403 403
Size: 5699
EcoO109I rG'GnC_Cy
Cuts at: 2838 2838
Size: 5699
EcoRI G'AATT_C
Cuts at: 2703 2703
Size: 5699
EcoRV GAT'ATC
Cuts at: 1107 2190 1107
Size: 1083 4616
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1876 2247 2373 3718 1876
Size: 371 126 1345 3857
Fragments arranged by size:
3857 1345 371 126
FspI TGC'GCA
Cuts at: 3480 3480
Size: 5699
HaeII r_GCGC'y
Cuts at: 1726 3133 3381 4403 5382 1726
Size: 1407 248 1022 979 2043
Fragments arranged by size:
2043 1407 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 154 186 504 536 1524 1556 1675 1707 2316
Size: 32 318 32 988 32 119 32 609
Cuts at: 2316 2348 3541 3571 3573 3603 154
Size: 32 1193 30 2 30 2250
Fragments arranged by size:
2250 1193 988 609 318 119 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1209 2738 1209
Size: 1529 4170
HindIII A'AGCT_T
Cuts at: 277 1153 277
Size: 876 4823
KasI G'GCGC_C
Cuts at: 3129 3377 3129
Size: 248 5451
KpnI G_GTAC'C
Cuts at: 2719 2719
Size: 5699
MluI A'CGCG_T
Cuts at: 5132 5132
Size: 5699
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 480 927 4370 4554 480
Size: 447 3443 184 1625
Fragments arranged by size:
3443 1625 447 184
MscI TGG'CCA
Cuts at: 3460 3460
Size: 5699
MslI CAynn'nnrTG
Cuts at: 1606 2605 3688 3815 1606
Size: 999 1083 127 3490
Fragments arranged by size:
3490 1083 999 127
MspA1I CmG'CkG
Cuts at: 840 1276 2810 3124 3484 4497 4742 840
Size: 436 1534 314 360 1013 245 1797
Fragments arranged by size:
1797 1534 1013 436 360 314 245
NaeI GCC'GGC
Cuts at: 2503 3880 2503
Size: 1377 4322
NarI GG'CG_CC
Cuts at: 3130 3378 3130
Size: 248 5451
NcoI C'CATG_G
Cuts at: 107 1292 2640 107
Size: 1185 1348 3166
Fragments arranged by size:
3166 1348 1185
NgoMIV G'CCGG_C
Cuts at: 2501 3878 2501
Size: 1377 4322
NsiI A_TGCA'T
Cuts at: 678 1271 678
Size: 593 5106
NspI r_CATG'y
Cuts at: 676 1044 1273 1705 1908 2212 2752 3252 4159
Size: 368 229 432 203 304 540 500 907
Cuts at: 4159 676
Size: 2216
Fragments arranged by size:
2216 907 540 500 432 368 304 229
203
PasI CC'CwG_GG
Cuts at: 1678 2488 1678
Size: 810 4889
PciI A'CATG_T
Cuts at: 1040 1701 1904 4155 1040
Size: 661 203 2251 2584
Fragments arranged by size:
2584 2251 661 203
PfoI T'CCnGG_A
Cuts at: 2067 2067
Size: 5699
PmeI GTTT'AAAC
Cuts at: 2698 2698
Size: 5699
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4838 4870 4838
Size: 32 5667
PshAI GACnn'nnGTC
Cuts at: 574 1877 574
Size: 1303 4396
PsiI TTA'TAA
Cuts at: 2167 2167
Size: 5699
PspXI vC'TCGA_Gb
Cuts at: 126 126
Size: 5699
PstI C_TGCA'G
Cuts at: 1018 1654 2246 2746 3431 1018
Size: 636 592 500 685 3286
Fragments arranged by size:
3286 685 636 592 500
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5699
PvuII CAG'CTG
Cuts at: 840 3124 3484 840
Size: 2284 360 3055
Fragments arranged by size:
3055 2284 360
RsrII CG'GwC_CG
Cuts at: 3894 3894
Size: 5699
SacI G_AGCT'C
Cuts at: 127 445 2713 127
Size: 318 2268 3113
Fragments arranged by size:
3113 2268 318
SalI G'TCGA_C
Cuts at: 2736 2736
Size: 5699
SapI GCTCTTCn'nnn_
Cuts at: 3722 3932 3722
Size: 210 5489
SbfI CC_TGCA'GG
Cuts at: 2746 2746
Size: 5699
ScaI AGT'ACT
Cuts at: 5216 5216
Size: 5699
SfcI C'TryA_G
Cuts at: 33 1014 1650 2242 2742 3427 4420 4611 33
Size: 981 636 592 500 685 993 191 1121
Fragments arranged by size:
1121 993 981 685 636 592 500 191
SfoI GGC'GCC
Cuts at: 3131 3379 3131
Size: 248 5451
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5699
SmaI CCC'GGG
Cuts at: 2721 2721
Size: 5699
SmlI C'TyrA_G
Cuts at: 126 280 2854 3769 4261 4523 126
Size: 154 2574 915 492 262 1302
Fragments arranged by size:
2574 1302 915 492 262 154
SpeI A'CTAG_T
Cuts at: 67 2422 67
Size: 2355 3344
SphI G_CATG'C
Cuts at: 1273 2212 2752 3252 1273
Size: 939 540 500 3720
Fragments arranged by size:
3720 939 540 500
SspI AAT'ATT
Cuts at: 267 636 2342 267
Size: 369 1706 3624
Fragments arranged by size:
3624 1706 369
StuI AGG'CCT
Cuts at: 1375 1375
Size: 5699
StyI C'CwwG_G
Cuts at: 107 1292 2640 2833 3187 107
Size: 1185 1348 193 354 2619
Fragments arranged by size:
2619 1348 1185 354 193
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2617 2665 3282 3415 3874 4083 2617
Size: 48 617 133 459 209 4233
Fragments arranged by size:
4233 617 459 209 133 48
TatI w'GTAC_w
Cuts at: 130 5214 130
Size: 5084 615
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5216 5248 5216
Size: 32 5667
Tth111I GACn'n_nGTC
Cuts at: 3496 3496
Size: 5699
XbaI T'CTAG_A
Cuts at: 2730 2730
Size: 5699
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3194 3194
Size: 5699
XhoI C'TCGA_G
Cuts at: 126 126
Size: 5699
XmaI C'CCGG_G
Cuts at: 2719 2719
Size: 5699
XmnI GAAnn'nnTTC
Cuts at: 954 1856 2318 954
Size: 902 462 4335
Fragments arranged by size:
4335 902 462
ZraI GAC'GTC
Cuts at: 2454 2454
Size: 5699
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PfoI PmeI PpiI PshAI PsiI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AhdI AloI ApaI AscI AvrII BglI BmtI
BsaXI BseRI BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I FseI FspAI HpaI MfeI NdeI NheI
NotI NruI PacI PflMI PmlI PpuMI PspOMI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI