Restriction map for
pF1KB8501
(Circular) (Six-base) MAPSORT of: pF1KB8501.seq Check: 7334 from: 1 to: 5921
pF1KB8501 5921 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 475 1152 2593 2959 475
Size: 677 1441 366 3437
Fragments arranged by size:
3437 1441 677 366
Acc65I G'GTAC_C
Cuts at: 2937 2937
Size: 5921
AclI AA'CG_TT
Cuts at: 5761 5761
Size: 5921
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1024 1754 2930 3746 4178 4925 5193 1024
Size: 730 1176 816 432 747 268 1752
Fragments arranged by size:
1752 1176 816 747 730 432 268
AflIII A'CryG_T
Cuts at: 2553 3273 4377 5354 2553
Size: 720 1104 977 3120
Fragments arranged by size:
3120 1104 977 720
AgeI A'CCGG_T
Cuts at: 4309 4309
Size: 5921
AhdI GACnn_n'nnGTC
Cuts at: 1956 1956
Size: 5921
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2207 2239 2207
Size: 32 5889
AlwNI CAG_nnn'CTG
Cuts at: 283 3226 4793 283
Size: 2943 1567 1411
Fragments arranged by size:
2943 1567 1411
ApaLI G'TGCA_C
Cuts at: 4691 4691
Size: 5921
ApoI r'AATT_y
Cuts at: 402 713 765 798 889 967 978 1319 1365
Size: 311 52 33 91 78 11 341 46
Cuts at: 1365 1594 1774 1800 2023 2301 2720 2925 5274
Size: 229 180 26 223 278 419 205 2349
Cuts at: 5274 5530 402
Size: 256 793
Fragments arranged by size:
2349 793 419 341 311 278 256 229
223 205 180 91 78 52 46 33
26 11
AseI AT'TA_AT
Cuts at: 20 1079 1514 2377 20
Size: 1059 435 863 3564
Fragments arranged by size:
3564 1059 863 435
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5921
AvaI C'yCGr_G
Cuts at: 1709 2941 1709
Size: 1232 4689
BamHI G'GATC_C
Cuts at: 1401 2820 2946 1401
Size: 1419 126 4376
Fragments arranged by size:
4376 1419 126
BanI G'GyrC_C
Cuts at: 2937 3351 3599 3634 2937
Size: 414 248 35 5224
Fragments arranged by size:
5224 414 248 35
BanII G_rGCy'C
Cuts at: 2935 3965 2935
Size: 1030 4891
BbeI G_GCGC'C
Cuts at: 3355 3603 3355
Size: 248 5673
BbsI GAAGACnn'nnnn_
Cuts at: 1475 1672 1475
Size: 197 5724
BbvCI CC'TCA_GC
Cuts at: 112 112
Size: 5921
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5887
BciVI GTATCCnnnnn_n'
Cuts at: 2528 3819 4580 5921 2528
Size: 1291 761 1341 2528
Fragments arranged by size:
2528 1341 1291 761
BclI T'GATC_A
Cuts at: 698 788 1758 2135 3441 698
Size: 90 970 377 1306 3178
Fragments arranged by size:
3178 1306 970 377 90
BfrBI ATG'CAT
Cuts at: 881 2538 881
Size: 1657 4264
BglII A'GATC_T
Cuts at: 122 3436 5473 122
Size: 3314 2037 570
Fragments arranged by size:
3314 2037 570
BlpI GC'TnA_GC
Cuts at: 3033 3033
Size: 5921
Bme1580I G_kGCm'C
Cuts at: 3546 3639 4695 3546
Size: 93 1056 4772
Fragments arranged by size:
4772 1056 93
BmgBI CAC'GTC
Cuts at: 275 275
Size: 5921
BmrI ACTGGGnnnn_n'
Cuts at: 660 3133 3214 3549 5693 660
Size: 2473 81 335 2144 888
Fragments arranged by size:
2473 2144 888 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4288 4288
Size: 5921
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 1163 196
Size: 967 4954
Bpu10I CC'TnA_GC
Cuts at: 112 112
Size: 5921
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 332 2553 2839 3097 3976 4468 4766 332
Size: 2221 286 258 879 492 298 1487
Fragments arranged by size:
2221 1487 879 492 298 286 258
BsaI GGTCTCn'nnnn_
Cuts at: 255 5625 255
Size: 5370 551
BsaAI yAC'GTr
Cuts at: 3274 3274
Size: 5921
BsaBI GATnn'nnATC
Cuts at: 6 3435 5472 6
Size: 3429 2037 455
Fragments arranged by size:
3429 2037 455
BsaHI Gr'CG_yC
Cuts at: 3352 3600 4302 5798 3352
Size: 248 702 1496 3475
Fragments arranged by size:
3475 1496 702 248
BsaWI w'CCGG_w
Cuts at: 293 3110 3631 4309 4583 4730 5141 293
Size: 2817 521 678 274 147 411 1073
Fragments arranged by size:
2817 1073 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 901 931 1122 1152 901
Size: 30 191 30 5670
Fragments arranged by size:
5670 191 30 30
BseYI C'CCAG_C
Cuts at: 3346 4104 4681 5177 3346
Size: 758 577 496 4090
Fragments arranged by size:
4090 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 416 542 1980 416
Size: 126 1438 4357
Fragments arranged by size:
4357 1438 126
BsiEI CG_ry'CG
Cuts at: 104 294 3463 3509 4717 104
Size: 190 3169 46 1208 1308
Fragments arranged by size:
3169 1308 1208 190 46
BsiHKAI G_wGCw'C
Cuts at: 2935 3713 3903 4695 2935
Size: 778 190 792 4161
Fragments arranged by size:
4161 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 2935 3546 3639 3713 3903 3965 4695 2935
Size: 611 93 74 190 62 730 4161
Fragments arranged by size:
4161 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 5141 5141
Size: 5921
BspHI T'CATG_A
Cuts at: 5097 5097
Size: 5921
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2971 3185 3487 3868 2971
Size: 214 302 381 5024
Fragments arranged by size:
5024 381 302 214
BsrBI CCG'CTC
Cuts at: 50 179 4213 4267 5751 5916 50
Size: 129 4034 54 1484 165 55
Fragments arranged by size:
4034 1484 165 129 55 54
BsrDI GCAATG_nn'
Cuts at: 1929 3833 1929
Size: 1904 4017
BsrFI r'CCGG_y
Cuts at: 3919 4100 4309 3919
Size: 181 209 5531
Fragments arranged by size:
5531 209 181
BssSI C'ACGA_G
Cuts at: 434 4192 4550 434
Size: 3758 358 1805
Fragments arranged by size:
3758 1805 358
BstAPI GCAn_nnn'nTGC
Cuts at: 943 3298 943
Size: 2355 3566
BstBI TT'CG_AA
Cuts at: 4282 4282
Size: 5921
BstYI r'GATC_y
Cuts at: 1 122 1401 2820 2946 3413 3436 3771 4017
Size: 121 1279 1419 126 467 23 335 246
Cuts at: 4017 5029 5115 5127 5473 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1419 1279 1012 467 449 346 335 246
126 121 86 23 12
BstZ17I GTA'TAC
Cuts at: 2594 2594
Size: 5921
Bsu36I CC'TnA_GG
Cuts at: 185 185
Size: 5921
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3871 4052 4215 3871
Size: 181 163 5577
Fragments arranged by size:
5577 181 163
BtsI GCAGTG_nn'
Cuts at: 1580 3164 1580
Size: 1584 4337
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2772 2807 2772
Size: 35 5886
DraI TTT'AAA
Cuts at: 641 1409 2177 2920 641
Size: 768 768 743 3642
Fragments arranged by size:
3642 768 768 743
DrdI GACnn_nn'nnGTC
Cuts at: 3627 4485 3627
Size: 858 5063
EaeI y'GGCC_r
Cuts at: 340 3506 3680 4071 4098 5443 340
Size: 3166 174 391 27 1345 818
Fragments arranged by size:
3166 1345 818 391 174 27
EagI C'GGCC_G
Cuts at: 3506 3506
Size: 5921
EarI CTCTTCn'nnn_
Cuts at: 2618 3944 4154 2618
Size: 1326 210 4385
Fragments arranged by size:
4385 1326 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4129 4439 4585 5731 4129
Size: 310 146 1146 4319
Fragments arranged by size:
4319 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2933 2933
Size: 5921
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 1024 1163 1754 2930 3746 4178 4925 5193
Size: 828 139 591 1176 816 432 747 268
Cuts at: 5193 196
Size: 924
Fragments arranged by size:
1176 924 828 816 747 591 432 268
139
EcoNI CCTnn'n_nnAGG
Cuts at: 907 907
Size: 5921
EcoO109I rG'GnC_Cy
Cuts at: 190 3060 190
Size: 2870 3051
EcoRI G'AATT_C
Cuts at: 765 889 2925 765
Size: 124 2036 3761
Fragments arranged by size:
3761 2036 124
EcoRV GAT'ATC
Cuts at: 1655 2292 1655
Size: 637 5284
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 443 1249 3940 443
Size: 806 2691 2424
Fragments arranged by size:
2691 2424 806
FspI TGC'GCA
Cuts at: 3702 3702
Size: 5921
HaeII r_GCGC'y
Cuts at: 3355 3603 4625 5604 3355
Size: 248 1022 979 3672
Fragments arranged by size:
3672 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 112 144 901 933 1036 1068 1706 1738 1942
Size: 32 757 32 103 32 638 32 204
Cuts at: 1942 1974 2644 2676 3763 3793 3795 3825 112
Size: 32 670 32 1087 30 2 30 2208
Fragments arranged by size:
2208 1087 757 670 638 204 103 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1203 2960 1203
Size: 1757 4164
HpaI GTT'AAC
Cuts at: 1203 1203
Size: 5921
KasI G'GCGC_C
Cuts at: 3351 3599 3351
Size: 248 5673
KpnI G_GTAC'C
Cuts at: 2941 2941
Size: 5921
MfeI C'AATT_G
Cuts at: 2675 2815 2675
Size: 140 5781
MluI A'CGCG_T
Cuts at: 5354 5354
Size: 5921
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4592 4776 4592
Size: 184 5737
MscI TGG'CCA
Cuts at: 3682 3682
Size: 5921
MslI CAynn'nnrTG
Cuts at: 1726 2507 3910 4037 1726
Size: 781 1403 127 3610
Fragments arranged by size:
3610 1403 781 127
MspA1I CmG'CkG
Cuts at: 940 3032 3346 3706 4719 4964 940
Size: 2092 314 360 1013 245 1897
Fragments arranged by size:
2092 1897 1013 360 314 245
NaeI GCC'GGC
Cuts at: 4102 4102
Size: 5921
NarI GG'CG_CC
Cuts at: 3352 3600 3352
Size: 248 5673
NdeI CA'TA_TG
Cuts at: 687 2220 687
Size: 1533 4388
NgoMIV G'CCGG_C
Cuts at: 4100 4100
Size: 5921
NsiI A_TGCA'T
Cuts at: 883 2540 883
Size: 1657 4264
NspI r_CATG'y
Cuts at: 2538 2557 2974 3474 4381 2538
Size: 19 417 500 907 4078
Fragments arranged by size:
4078 907 500 417 19
PacI TTA_AT'TAA
Cuts at: 1079 1832 1079
Size: 753 5168
PciI A'CATG_T
Cuts at: 2553 4377 2553
Size: 1824 4097
PfoI T'CCnGG_A
Cuts at: 212 1140 2489 212
Size: 928 1349 3644
Fragments arranged by size:
3644 1349 928
PmeI GTTT'AAAC
Cuts at: 2920 2920
Size: 5921
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5060 5092 5060
Size: 32 5889
PpuMI rG'GwC_Cy
Cuts at: 190 190
Size: 5921
PsiI TTA'TAA
Cuts at: 503 503
Size: 5921
PspXI vC'TCGA_Gb
Cuts at: 1709 1709
Size: 5921
PstI C_TGCA'G
Cuts at: 2652 2968 3653 2652
Size: 316 685 4920
Fragments arranged by size:
4920 685 316
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5921
PvuII CAG'CTG
Cuts at: 940 3346 3706 940
Size: 2406 360 3155
Fragments arranged by size:
3155 2406 360
RsrII CG'GwC_CG
Cuts at: 4116 4116
Size: 5921
SacI G_AGCT'C
Cuts at: 2935 2935
Size: 5921
SalI G'TCGA_C
Cuts at: 2958 2958
Size: 5921
SanDI GG'GwC_CC
Cuts at: 190 190
Size: 5921
SapI GCTCTTCn'nnn_
Cuts at: 3944 4154 3944
Size: 210 5711
SbfI CC_TGCA'GG
Cuts at: 2968 2968
Size: 5921
ScaI AGT'ACT
Cuts at: 2280 5438 2280
Size: 3158 2763
SfcI C'TryA_G
Cuts at: 33 1092 1733 2648 2964 3649 4642 4833 33
Size: 1059 641 915 316 685 993 191 1121
Fragments arranged by size:
1121 1059 993 915 685 641 316 191
SfoI GGC'GCC
Cuts at: 3353 3601 3353
Size: 248 5673
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5921
SmaI CCC'GGG
Cuts at: 2943 2943
Size: 5921
SmlI C'TyrA_G
Cuts at: 347 1709 2568 2854 3076 3991 4483 4745 347
Size: 1362 859 286 222 915 492 262 1523
Fragments arranged by size:
1523 1362 915 859 492 286 262 222
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5921
SphI G_CATG'C
Cuts at: 2974 3474 2974
Size: 500 5421
SspI AAT'ATT
Cuts at: 2236 2236
Size: 5921
StuI AGG'CCT
Cuts at: 117 117
Size: 5921
StyI C'CwwG_G
Cuts at: 1388 1463 2690 3055 3409 1388
Size: 75 1227 365 354 3900
Fragments arranged by size:
3900 1227 365 354 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 228 3504 3637 4096 4305 228
Size: 3276 133 459 209 1844
Fragments arranged by size:
3276 1844 459 209 133
TatI w'GTAC_w
Cuts at: 863 2278 2864 5436 863
Size: 1415 586 2572 1348
Fragments arranged by size:
2572 1415 1348 586
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5438 5470 5438
Size: 32 5889
Tth111I GACn'n_nGTC
Cuts at: 1336 3718 1336
Size: 2382 3539
XbaI T'CTAG_A
Cuts at: 2952 2952
Size: 5921
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3416 3416
Size: 5921
XhoI C'TCGA_G
Cuts at: 1709 1709
Size: 5921
XmaI C'CCGG_G
Cuts at: 2941 2941
Size: 5921
XmnI GAAnn'nnTTC
Cuts at: 838 971 1232 1925 2876 2894 838
Size: 133 261 693 951 18 3865
Fragments arranged by size:
3865 951 693 261 133 18
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BstZ17I Bsu36I
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NsiI NspI PacI PciI PfoI PmeI PpiI
PpuMI PsiI PspXI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI ApaI AscI
AvrII BaeI BglI BmtI BseRI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BtgI ClaI DraIII FseI
FspAI HindIII NcoI NheI NotI NruI PasI PflMI
PmlI PshAI PspOMI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SwaI ZraI