Restriction map for
pF1KB8507
(Circular) (Six-base) MAPSORT of: pF1KB8507.seq Check: 1190 from: 1 to: 5936
pF1KB8507 5936 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 213 2692 213
Size: 2479 3457
AatII G_ACGT'C
Cuts at: 2093 2093
Size: 5936
AccI GT'mk_AC
Cuts at: 872 2974 872
Size: 2102 3834
Acc65I G'GTAC_C
Cuts at: 1649 2492 2952 1649
Size: 843 460 4633
Fragments arranged by size:
4633 843 460
AclI AA'CG_TT
Cuts at: 5776 5776
Size: 5936
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 1675 1819 2089 3761 4193 4940 5208 406
Size: 1269 144 270 1672 432 747 268 1134
Fragments arranged by size:
1672 1269 1134 747 432 270 268 144
AfeI AGC'GCT
Cuts at: 1747 1747
Size: 5936
AflIII A'CryG_T
Cuts at: 3288 4392 5369 3288
Size: 1104 977 3855
Fragments arranged by size:
3855 1104 977
AgeI A'CCGG_T
Cuts at: 4324 4324
Size: 5936
AleI CACnn'nnGTG
Cuts at: 1940 1940
Size: 5936
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 727 759 829 861 727
Size: 32 70 32 5802
Fragments arranged by size:
5802 70 32 32
AlwNI CAG_nnn'CTG
Cuts at: 840 1251 1434 1797 2385 2579 3241 4808 840
Size: 411 183 363 588 194 662 1567 1968
Fragments arranged by size:
1968 1567 662 588 411 363 194 183
ApaI G_GGCC'C
Cuts at: 902 2013 2727 902
Size: 1111 714 4111
Fragments arranged by size:
4111 1111 714
ApaLI G'TGCA_C
Cuts at: 4706 4706
Size: 5936
ApoI r'AATT_y
Cuts at: 2940 5289 5545 2940
Size: 2349 256 3331
Fragments arranged by size:
3331 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5936
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5936
AvaI C'yCGr_G
Cuts at: 193 623 2956 193
Size: 430 2333 3173
Fragments arranged by size:
3173 2333 430
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 361 394 518 551 361
Size: 33 124 33 5746
Fragments arranged by size:
5746 124 33 33
BamHI G'GATC_C
Cuts at: 214 2022 2961 214
Size: 1808 939 3189
Fragments arranged by size:
3189 1808 939
BanI G'GyrC_C
Cuts at: 1649 2492 2952 3366 3614 3649 1649
Size: 843 460 414 248 35 3936
Fragments arranged by size:
3936 843 460 414 248 35
BanII G_rGCy'C
Cuts at: 902 1024 1123 2013 2537 2727 2950 3980 902
Size: 122 99 890 524 190 223 1030 2858
Fragments arranged by size:
2858 1030 890 524 223 190 122 99
BbeI G_GCGC'C
Cuts at: 3370 3618 3370
Size: 248 5688
BbsI GAAGACnn'nnnn_
Cuts at: 1676 1676
Size: 5936
BbvCI CC'TCA_GC
Cuts at: 2278 2278
Size: 5936
BciVI GTATCCnnnnn_n'
Cuts at: 2524 3834 4595 5936 2524
Size: 1310 761 1341 2524
Fragments arranged by size:
2524 1341 1310 761
BclI T'GATC_A
Cuts at: 2751 3456 2751
Size: 705 5231
BglI GCCn_nnn'nGGC
Cuts at: 112 1835 112
Size: 1723 4213
BglII A'GATC_T
Cuts at: 308 449 2742 3451 5488 308
Size: 141 2293 709 2037 756
Fragments arranged by size:
2293 2037 756 709 141
BlpI GC'TnA_GC
Cuts at: 172 813 3048 172
Size: 641 2235 3060
Fragments arranged by size:
3060 2235 641
Bme1580I G_kGCm'C
Cuts at: 902 2013 2727 2929 3561 3654 4710 902
Size: 1111 714 202 632 93 1056 2128
Fragments arranged by size:
2128 1111 1056 714 632 202 93
BmrI ACTGGGnnnn_n'
Cuts at: 2016 3148 3229 3564 5708 2016
Size: 1132 81 335 2144 2244
Fragments arranged by size:
2244 2144 1132 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1137 1315 2619 2673 4303 1137
Size: 178 1304 54 1630 2770
Fragments arranged by size:
2770 1630 1304 178 54
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 481 1210 1537 2859 2901 2902 481
Size: 729 327 1322 42 1 3515
Fragments arranged by size:
3515 1322 729 327 42 1
Bpu10I CC'TnA_GC
Cuts at: 2278 2770 2278
Size: 492 5444
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1483 2008 2317 2521 3112 3991 4483 4781 1483
Size: 525 309 204 591 879 492 298 2638
Fragments arranged by size:
2638 879 591 525 492 309 298 204
BsaI GGTCTCn'nnnn_
Cuts at: 1286 2497 5640 1286
Size: 1211 3143 1582
Fragments arranged by size:
3143 1582 1211
BsaAI yAC'GTr
Cuts at: 1131 1374 3289 1131
Size: 243 1915 3778
Fragments arranged by size:
3778 1915 243
BsaBI GATnn'nnATC
Cuts at: 6 3450 5487 6
Size: 3444 2037 455
Fragments arranged by size:
3444 2037 455
BsaHI Gr'CG_yC
Cuts at: 2090 3367 3615 4317 5813 2090
Size: 1277 248 702 1496 2213
Fragments arranged by size:
2213 1496 1277 702 248
BsaWI w'CCGG_w
Cuts at: 974 2699 3125 3646 4324 4598 4745 5156 974
Size: 1725 426 521 678 274 147 411 1754
Fragments arranged by size:
1754 1725 678 521 426 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1183 1213 2729 2759 2838 2868 1183
Size: 30 1516 30 79 30 4251
Fragments arranged by size:
4251 1516 79 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1468 1534 1894 2314 2515 1468
Size: 66 360 420 201 4889
Fragments arranged by size:
4889 420 360 201 66
BseYI C'CCAG_C
Cuts at: 1397 1825 2887 3361 4119 4696 5192 1397
Size: 428 1062 474 758 577 496 2141
Fragments arranged by size:
2141 1062 758 577 496 474 428
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 474 1420 1969 2398 2592 474
Size: 946 549 429 194 3818
Fragments arranged by size:
3818 946 549 429 194
BsiEI CG_ry'CG
Cuts at: 104 3478 3524 4732 104
Size: 3374 46 1208 1308
Fragments arranged by size:
3374 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1024 1937 2537 2779 2950 3728 3918 4710 1024
Size: 913 600 242 171 778 190 792 2250
Fragments arranged by size:
2250 913 792 778 600 242 190 171
BsmI GAATG_Cn'
Cuts at: 1403 1403
Size: 5936
Bsp1286I G_dGCh'C
Cuts at: 902 1024 1123 1937 2013 2537 2727 2779 2929
Size: 122 99 814 76 524 190 52 150
Cuts at: 2929 2950 3561 3654 3728 3918 3980 4710 902
Size: 21 611 93 74 190 62 730 2128
Fragments arranged by size:
2128 814 730 611 524 190 190 150
122 99 93 76 74 62 52 21
BspEI T'CCGG_A
Cuts at: 5156 5156
Size: 5936
BspHI T'CATG_A
Cuts at: 1641 5112 1641
Size: 3471 2465
BspMI ACCTGCnnnn'nnnn_
Cuts at: 128 213 848 1433 2208 2692 2986 3200 3502
Size: 85 635 585 775 484 294 214 302
Cuts at: 3502 3883 128
Size: 381 2181
Fragments arranged by size:
2181 775 635 585 484 381 302 294
214 85
BsrBI CCG'CTC
Cuts at: 50 190 4228 4282 5766 5931 50
Size: 140 4038 54 1484 165 55
Fragments arranged by size:
4038 1484 165 140 55 54
BsrDI GCAATG_nn'
Cuts at: 183 929 3848 183
Size: 746 2919 2271
Fragments arranged by size:
2919 2271 746
BsrFI r'CCGG_y
Cuts at: 629 2213 3934 4115 4324 629
Size: 1584 1721 181 209 2241
Fragments arranged by size:
2241 1721 1584 209 181
BssSI C'ACGA_G
Cuts at: 2014 2203 4207 4565 2014
Size: 189 2004 358 3385
Fragments arranged by size:
3385 2004 358 189
BstAPI GCAn_nnn'nTGC
Cuts at: 1434 2184 3313 1434
Size: 750 1129 4057
Fragments arranged by size:
4057 1129 750
BstBI TT'CG_AA
Cuts at: 4297 4297
Size: 5936
BstXI CCAn_nnnn'nTGG
Cuts at: 799 799
Size: 5936
BstYI r'GATC_y
Cuts at: 1 214 308 449 1658 1757 1970 2022 2525
Size: 213 94 141 1209 99 213 52 503
Cuts at: 2525 2742 2961 3428 3451 3786 4032 5044 5130
Size: 217 219 467 23 335 246 1012 86
Cuts at: 5130 5142 5488 1
Size: 12 346 449
Fragments arranged by size:
1209 1012 503 467 449 346 335 246
219 217 213 213 141 99 94 86
52 23 12
BtgI C'CryG_G
Cuts at: 107 792 2241 107
Size: 685 1449 3802
Fragments arranged by size:
3802 1449 685
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3886 4067 4230 3886
Size: 181 163 5592
Fragments arranged by size:
5592 181 163
BtsI GCAGTG_nn'
Cuts at: 130 1159 2640 3179 130
Size: 1029 1481 539 2887
Fragments arranged by size:
2887 1481 1029 539
DraI TTT'AAA
Cuts at: 2935 2935
Size: 5936
DrdI GACnn_nn'nnGTC
Cuts at: 2431 3642 4500 2431
Size: 1211 858 3867
Fragments arranged by size:
3867 1211 858
EaeI y'GGCC_r
Cuts at: 572 854 1451 2622 3521 3695 4086 4113 5458
Size: 282 597 1171 899 174 391 27 1345
Cuts at: 5458 572
Size: 1050
Fragments arranged by size:
1345 1171 1050 899 597 391 282 174
27
EagI C'GGCC_G
Cuts at: 3521 3521
Size: 5936
EarI CTCTTCn'nnn_
Cuts at: 419 460 2065 2372 3959 4169 419
Size: 41 1605 307 1587 210 2186
Fragments arranged by size:
2186 1605 1587 307 210 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1639 2506 4144 4454 4600 5746 1639
Size: 867 1638 310 146 1146 1829
Fragments arranged by size:
1829 1638 1146 867 310 146
EcoICRI GAG'CTC
Cuts at: 1022 2535 2948 1022
Size: 1513 413 4010
Fragments arranged by size:
4010 1513 413
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 481 1210 1537 1675 1819 2089 2859 2901
Size: 75 729 327 138 144 270 770 42
Cuts at: 2901 2902 3761 4193 4940 5208 406
Size: 1 859 432 747 268 1134
Fragments arranged by size:
1134 859 770 747 729 432 327 270
268 144 138 75 42 1
EcoNI CCTnn'n_nnAGG
Cuts at: 1588 1588
Size: 5936
EcoO109I rG'GnC_Cy
Cuts at: 837 899 1581 1854 2103 2253 2274 3075 837
Size: 62 682 273 249 150 21 801 3698
Fragments arranged by size:
3698 801 682 273 249 150 62 21
EcoRI G'AATT_C
Cuts at: 2940 2940
Size: 5936
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 845 2059 3955 845
Size: 1214 1896 2826
Fragments arranged by size:
2826 1896 1214
FspI TGC'GCA
Cuts at: 3717 3717
Size: 5936
HaeII r_GCGC'y
Cuts at: 135 1749 2241 3370 3618 4640 5619 135
Size: 1614 492 1129 248 1022 979 452
Fragments arranged by size:
1614 1129 1022 979 492 452 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1233 1265 2838 2870 3778 3808 3810 3840 1233
Size: 32 1573 32 908 30 2 30 3329
Fragments arranged by size:
3329 1573 908 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2975 2975
Size: 5936
HindIII A'AGCT_T
Cuts at: 2041 2041
Size: 5936
KasI G'GCGC_C
Cuts at: 3366 3614 3366
Size: 248 5688
KpnI G_GTAC'C
Cuts at: 1653 2496 2956 1653
Size: 843 460 4633
Fragments arranged by size:
4633 843 460
MluI A'CGCG_T
Cuts at: 5369 5369
Size: 5936
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 393 764 1049 1340 4607 4791 393
Size: 371 285 291 3267 184 1538
Fragments arranged by size:
3267 1538 371 291 285 184
MscI TGG'CCA
Cuts at: 574 1453 2624 3697 574
Size: 879 1171 1073 2813
Fragments arranged by size:
2813 1171 1073 879
MslI CAynn'nnrTG
Cuts at: 797 1559 1940 2227 2713 3925 4052 797
Size: 762 381 287 486 1212 127 2681
Fragments arranged by size:
2681 1212 762 486 381 287 127
MspA1I CmG'CkG
Cuts at: 171 220 2326 3047 3361 3721 4734 4979 171
Size: 49 2106 721 314 360 1013 245 1128
Fragments arranged by size:
2106 1128 1013 721 360 314 245 49
NaeI GCC'GGC
Cuts at: 4117 4117
Size: 5936
NarI GG'CG_CC
Cuts at: 3367 3615 3367
Size: 248 5688
NcoI C'CATG_G
Cuts at: 107 792 2241 107
Size: 685 1449 3802
Fragments arranged by size:
3802 1449 685
NgoMIV G'CCGG_C
Cuts at: 4115 4115
Size: 5936
NspI r_CATG'y
Cuts at: 2199 2989 3489 4396 2199
Size: 790 500 907 3739
Fragments arranged by size:
3739 907 790 500
PasI CC'CwG_GG
Cuts at: 1965 1965
Size: 5936
PciI A'CATG_T
Cuts at: 4392 4392
Size: 5936
PflMI CCAn_nnn'nTGG
Cuts at: 1251 1251
Size: 5936
PfoI T'CCnGG_A
Cuts at: 513 2132 2917 513
Size: 1619 785 3532
Fragments arranged by size:
3532 1619 785
PmeI GTTT'AAAC
Cuts at: 2935 2935
Size: 5936
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5075 5107 5075
Size: 32 5904
PpuMI rG'GwC_Cy
Cuts at: 837 1581 2253 837
Size: 744 672 4520
Fragments arranged by size:
4520 744 672
PspOMI G'GGCC_C
Cuts at: 898 2009 2723 898
Size: 1111 714 4111
Fragments arranged by size:
4111 1111 714
PstI C_TGCA'G
Cuts at: 125 845 1166 1430 1867 2003 2983 3668 125
Size: 720 321 264 437 136 980 685 2393
Fragments arranged by size:
2393 980 720 685 437 321 264 136
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5936
PvuII CAG'CTG
Cuts at: 2326 3361 3721 2326
Size: 1035 360 4541
Fragments arranged by size:
4541 1035 360
RsrII CG'GwC_CG
Cuts at: 4131 4131
Size: 5936
SacI G_AGCT'C
Cuts at: 1024 2537 2950 1024
Size: 1513 413 4010
Fragments arranged by size:
4010 1513 413
SalI G'TCGA_C
Cuts at: 2973 2973
Size: 5936
SapI GCTCTTCn'nnn_
Cuts at: 3959 4169 3959
Size: 210 5726
SbfI CC_TGCA'GG
Cuts at: 2983 2983
Size: 5936
ScaI AGT'ACT
Cuts at: 5453 5453
Size: 5936
SexAI A'CCwGG_T
Cuts at: 2429 2429
Size: 5936
SfcI C'TryA_G
Cuts at: 33 121 331 841 1074 1162 1302 1426 1863
Size: 88 210 510 233 88 140 124 437
Cuts at: 1863 1999 2443 2979 3664 4657 4848 33
Size: 136 444 536 685 993 191 1121
Fragments arranged by size:
1121 993 685 536 510 444 437 233
210 191 140 136 124 88 88
SfoI GGC'GCC
Cuts at: 3368 3616 3368
Size: 248 5688
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5936
SmaI CCC'GGG
Cuts at: 195 625 2958 195
Size: 430 2333 3173
Fragments arranged by size:
3173 2333 430
SmlI C'TyrA_G
Cuts at: 1498 1987 2332 2536 3091 4006 4498 4760 1498
Size: 489 345 204 555 915 492 262 2674
Fragments arranged by size:
2674 915 555 492 489 345 262 204
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5936
SphI G_CATG'C
Cuts at: 2989 3489 2989
Size: 500 5436
SspI AAT'ATT
Cuts at: 399 399
Size: 5936
StyI C'CwwG_G
Cuts at: 107 440 792 2241 2591 3070 3424 107
Size: 333 352 1449 350 479 354 2619
Fragments arranged by size:
2619 1449 479 354 352 350 333
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 805 2863 3519 3652 4111 4320 805
Size: 2058 656 133 459 209 2421
Fragments arranged by size:
2421 2058 656 459 209 133
TatI w'GTAC_w
Cuts at: 1810 5451 1810
Size: 3641 2295
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1429 1461 5453 5485 1429
Size: 32 3992 32 1880
Fragments arranged by size:
3992 1880 32 32
Tth111I GACn'n_nGTC
Cuts at: 3733 3733
Size: 5936
XbaI T'CTAG_A
Cuts at: 2967 2967
Size: 5936
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1356 2617 3431 1356
Size: 1261 814 3861
Fragments arranged by size:
3861 1261 814
XmaI C'CCGG_G
Cuts at: 193 623 2956 193
Size: 430 2333 3173
Fragments arranged by size:
3173 2333 430
XmnI GAAnn'nnTTC
Cuts at: 1694 1924 2170 1694
Size: 230 246 5460
Fragments arranged by size:
5460 246 230
ZraI GAC'GTC
Cuts at: 2091 2091
Size: 5936
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AlfI AscI AvrII BcgI BfrBI BmgBI
BmtI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI