Restriction map for
pF1KB8508
(Circular) (Six-base) MAPSORT of: pF1KB8508.seq Check: 1262 from: 1 to: 5711
pF1KB8508 5711 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AccI GT'mk_AC
Cuts at: 2749 2749
Size: 5711
Acc65I G'GTAC_C
Cuts at: 837 2032 2727 837
Size: 1195 695 3821
Fragments arranged by size:
3821 1195 695
AclI AA'CG_TT
Cuts at: 5551 5551
Size: 5711
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 550 709 814 2012 2372 3536 3968 4715
Size: 335 159 105 1198 360 1164 432 747
Cuts at: 4715 4983 215
Size: 268 943
Fragments arranged by size:
1198 1164 943 747 432 360 335 268
159 105
AflIII A'CryG_T
Cuts at: 1477 3063 4167 5144 1477
Size: 1586 1104 977 2044
Fragments arranged by size:
2044 1586 1104 977
AgeI A'CCGG_T
Cuts at: 4099 4099
Size: 5711
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1173 1205 1173
Size: 32 5679
AlwNI CAG_nnn'CTG
Cuts at: 570 1306 1759 2000 3016 4583 570
Size: 736 453 241 1016 1567 1698
Fragments arranged by size:
1698 1567 1016 736 453 241
ApaLI G'TGCA_C
Cuts at: 4481 4481
Size: 5711
ApoI r'AATT_y
Cuts at: 375 440 813 1813 2157 2224 2715 5064 5320
Size: 65 373 1000 344 67 491 2349 256
Cuts at: 5320 375
Size: 766
Fragments arranged by size:
2349 1000 766 491 373 344 256 67
65
AseI AT'TA_AT
Cuts at: 20 20
Size: 5711
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5711
AvaI C'yCGr_G
Cuts at: 917 1582 2731 917
Size: 665 1149 3897
Fragments arranged by size:
3897 1149 665
BamHI G'GATC_C
Cuts at: 2068 2736 2068
Size: 668 5043
BanI G'GyrC_C
Cuts at: 837 1643 2032 2727 3141 3389 3424 837
Size: 806 389 695 414 248 35 3124
Fragments arranged by size:
3124 806 695 414 389 248 35
BanII G_rGCy'C
Cuts at: 591 1006 1045 1084 1123 1162 1240 1433 1753
Size: 415 39 39 39 39 78 193 320
Cuts at: 1753 2272 2584 2725 3755 591
Size: 519 312 141 1030 2547
Fragments arranged by size:
2547 1030 519 415 320 312 193 141
78 39 39 39 39
BbeI G_GCGC'C
Cuts at: 3145 3393 3145
Size: 248 5463
BbsI GAAGACnn'nnnn_
Cuts at: 287 287
Size: 5711
BbvCI CC'TCA_GC
Cuts at: 462 462
Size: 5711
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 638 672 638
Size: 34 5677
BciVI GTATCCnnnnn_n'
Cuts at: 3609 4370 5711 3609
Size: 761 1341 3609
Fragments arranged by size:
3609 1341 761
BclI T'GATC_A
Cuts at: 3231 3231
Size: 5711
BglI GCCn_nnn'nGGC
Cuts at: 305 305
Size: 5711
BglII A'GATC_T
Cuts at: 684 3226 5263 684
Size: 2542 2037 1132
Fragments arranged by size:
2542 2037 1132
BlpI GC'TnA_GC
Cuts at: 2823 2823
Size: 5711
Bme1580I G_kGCm'C
Cuts at: 154 1223 1342 3336 3429 4485 154
Size: 1069 119 1994 93 1056 1380
Fragments arranged by size:
1994 1380 1069 1056 119 93
BmrI ACTGGGnnnn_n'
Cuts at: 1344 2307 2923 3004 3339 5483 1344
Size: 963 616 81 335 2144 1572
Fragments arranged by size:
2144 1572 963 616 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2457 4078 2457
Size: 1621 4090
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 634 1073 634
Size: 439 5272
Bpu10I CC'TnA_GC
Cuts at: 462 1432 462
Size: 970 4741
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2887 3766 4258 4556 2887
Size: 879 492 298 4042
Fragments arranged by size:
4042 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 888 2098 5415 888
Size: 1210 3317 1184
Fragments arranged by size:
3317 1210 1184
BsaAI yAC'GTr
Cuts at: 3064 3064
Size: 5711
BsaBI GATnn'nnATC
Cuts at: 6 3225 5262 6
Size: 3219 2037 455
Fragments arranged by size:
3219 2037 455
BsaHI Gr'CG_yC
Cuts at: 3142 3390 4092 5588 3142
Size: 248 702 1496 3265
Fragments arranged by size:
3265 1496 702 248
BsaWI w'CCGG_w
Cuts at: 943 1177 2165 2900 3421 4099 4373 4520 4931
Size: 234 988 735 521 678 274 147 411
Cuts at: 4931 943
Size: 1723
Fragments arranged by size:
1723 988 735 678 521 411 274 234
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 191 221 1712 1742 191
Size: 30 1491 30 4160
Fragments arranged by size:
4160 1491 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 215 935 1895 2364 215
Size: 720 960 469 3562
Fragments arranged by size:
3562 960 720 469
BseYI C'CCAG_C
Cuts at: 1216 2302 3136 3894 4471 4967 1216
Size: 1086 834 758 577 496 1960
Fragments arranged by size:
1960 1086 834 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2525 2525
Size: 5711
BsiEI CG_ry'CG
Cuts at: 104 3253 3299 4507 104
Size: 3149 46 1208 1308
Fragments arranged by size:
3149 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 591 1006 1045 1084 1123 1240 1433 2725 3503
Size: 415 39 39 39 117 193 1292 778
Cuts at: 3503 3693 4485 591
Size: 190 792 1817
Fragments arranged by size:
1817 1292 792 778 415 193 190 117
39 39 39
BsmBI CGTCTCn'nnnn_
Cuts at: 946 946
Size: 5711
Bsp1286I G_dGCh'C
Cuts at: 154 591 1006 1045 1084 1123 1162 1223 1240
Size: 437 415 39 39 39 39 61 17
Cuts at: 1240 1342 1433 1753 2272 2584 2725 3336 3429
Size: 102 91 320 519 312 141 611 93
Cuts at: 3429 3503 3693 3755 4485 154
Size: 74 190 62 730 1380
Fragments arranged by size:
1380 730 611 519 437 415 320 312
190 141 102 93 91 74 62 61
39 39 39 39 17
BspEI T'CCGG_A
Cuts at: 4931 4931
Size: 5711
BspHI T'CATG_A
Cuts at: 1944 4887 1944
Size: 2943 2768
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2761 2975 3277 3658 2761
Size: 214 302 381 4814
Fragments arranged by size:
4814 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4003 4057 5541 5706 50
Size: 3953 54 1484 165 55
Fragments arranged by size:
3953 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 728 1595 2244 3623 728
Size: 867 649 1379 2816
Fragments arranged by size:
2816 1379 867 649
BsrFI r'CCGG_y
Cuts at: 3709 3890 4099 3709
Size: 181 209 5321
Fragments arranged by size:
5321 209 181
BssSI C'ACGA_G
Cuts at: 674 1866 3982 4340 674
Size: 1192 2116 358 2045
Fragments arranged by size:
2116 2045 1192 358
BstAPI GCAn_nnn'nTGC
Cuts at: 163 3088 163
Size: 2925 2786
BstBI TT'CG_AA
Cuts at: 4072 4072
Size: 5711
BstEII G'GTnAC_C
Cuts at: 1452 1554 1452
Size: 102 5609
BstXI CCAn_nnnn'nTGG
Cuts at: 1153 1551 1153
Size: 398 5313
BstYI r'GATC_y
Cuts at: 1 504 635 684 2068 2736 3203 3226 3561
Size: 503 131 49 1384 668 467 23 335
Cuts at: 3561 3807 4819 4905 4917 5263 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1384 1012 668 503 467 449 346 335
246 131 86 49 23 12
Bsu36I CC'TnA_GG
Cuts at: 2665 2665
Size: 5711
BtgI C'CryG_G
Cuts at: 1824 1824
Size: 5711
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3661 3842 4005 3661
Size: 181 163 5367
Fragments arranged by size:
5367 181 163
BtsI GCAGTG_nn'
Cuts at: 984 1670 2387 2954 984
Size: 686 717 567 3741
Fragments arranged by size:
3741 717 686 567
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 206 241 206
Size: 35 5676
DraI TTT'AAA
Cuts at: 2710 2710
Size: 5711
DrdI GACnn_nn'nnGTC
Cuts at: 3417 4275 3417
Size: 858 4853
EaeI y'GGCC_r
Cuts at: 3296 3470 3861 3888 5233 3296
Size: 174 391 27 1345 3774
Fragments arranged by size:
3774 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3296 3296
Size: 5711
EarI CTCTTCn'nnn_
Cuts at: 1343 2489 3734 3944 1343
Size: 1146 1245 210 3110
Fragments arranged by size:
3110 1245 1146 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1624 3919 4229 4375 5521 1624
Size: 2295 310 146 1146 1814
Fragments arranged by size:
2295 1814 1146 310 146
EcoICRI GAG'CTC
Cuts at: 589 1004 1043 1082 1121 1238 1431 2723 589
Size: 415 39 39 39 117 193 1292 3577
Fragments arranged by size:
3577 1292 415 193 117 39 39 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 550 634 709 814 1073 2012 2372 3536
Size: 335 84 75 105 259 939 360 1164
Cuts at: 3536 3968 4715 4983 215
Size: 432 747 268 943
Fragments arranged by size:
1164 943 939 747 432 360 335 268
259 105 84 75
EcoNI CCTnn'n_nnAGG
Cuts at: 1715 2472 1715
Size: 757 4954
EcoO109I rG'GnC_Cy
Cuts at: 2690 2850 2690
Size: 160 5551
EcoRI G'AATT_C
Cuts at: 1813 2224 2715 1813
Size: 411 491 4809
Fragments arranged by size:
4809 491 411
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 760 3730 760
Size: 2970 2741
FspI TGC'GCA
Cuts at: 3492 3492
Size: 5711
HaeII r_GCGC'y
Cuts at: 3145 3393 4415 5394 3145
Size: 248 1022 979 3462
Fragments arranged by size:
3462 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 445 477 1660 1692 1712 1726 1744 1758 3553
Size: 32 1183 32 20 14 18 14 1795
Cuts at: 3553 3583 3585 3615 445
Size: 30 2 30 2541
Fragments arranged by size:
2541 1795 1183 32 32 30 30 20
18 14 14 2
HincII GTy'rAC
Cuts at: 422 2750 422
Size: 2328 3383
HindIII A'AGCT_T
Cuts at: 2312 2312
Size: 5711
KasI G'GCGC_C
Cuts at: 3141 3389 3141
Size: 248 5463
KpnI G_GTAC'C
Cuts at: 841 2036 2731 841
Size: 1195 695 3821
Fragments arranged by size:
3821 1195 695
MluI A'CGCG_T
Cuts at: 1477 5144 1477
Size: 3667 2044
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4382 4566 4382
Size: 184 5527
MscI TGG'CCA
Cuts at: 3472 3472
Size: 5711
MslI CAynn'nnrTG
Cuts at: 1649 3700 3827 1649
Size: 2051 127 3533
Fragments arranged by size:
3533 2051 127
MspA1I CmG'CkG
Cuts at: 2822 3136 3496 4509 4754 2822
Size: 314 360 1013 245 3779
Fragments arranged by size:
3779 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3892 3892
Size: 5711
NarI GG'CG_CC
Cuts at: 3142 3390 3142
Size: 248 5463
NgoMIV G'CCGG_C
Cuts at: 3890 3890
Size: 5711
NspI r_CATG'y
Cuts at: 870 2457 2764 3264 4171 870
Size: 1587 307 500 907 2410
Fragments arranged by size:
2410 1587 907 500 307
PasI CC'CwG_GG
Cuts at: 1036 1153 1801 2575 1036
Size: 117 648 774 4172
Fragments arranged by size:
4172 774 648 117
PciI A'CATG_T
Cuts at: 4167 4167
Size: 5711
PflMI CCAn_nnn'nTGG
Cuts at: 1306 1306
Size: 5711
PfoI T'CCnGG_A
Cuts at: 807 807
Size: 5711
PmeI GTTT'AAAC
Cuts at: 2710 2710
Size: 5711
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4850 4882 4850
Size: 32 5679
PspXI vC'TCGA_Gb
Cuts at: 917 917
Size: 5711
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1119 1151 1119
Size: 32 5679
PstI C_TGCA'G
Cuts at: 168 313 575 2758 3443 168
Size: 145 262 2183 685 2436
Fragments arranged by size:
2436 2183 685 262 145
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5711
PvuII CAG'CTG
Cuts at: 3136 3496 3136
Size: 360 5351
RsrII CG'GwC_CG
Cuts at: 3906 3906
Size: 5711
SacI G_AGCT'C
Cuts at: 591 1006 1045 1084 1123 1240 1433 2725 591
Size: 415 39 39 39 117 193 1292 3577
Fragments arranged by size:
3577 1292 415 193 117 39 39 39
SalI G'TCGA_C
Cuts at: 2748 2748
Size: 5711
SapI GCTCTTCn'nnn_
Cuts at: 3734 3944 3734
Size: 210 5501
SbfI CC_TGCA'GG
Cuts at: 2758 2758
Size: 5711
ScaI AGT'ACT
Cuts at: 1020 1098 1137 1254 5228 1020
Size: 78 39 117 3974 1503
Fragments arranged by size:
3974 1503 117 78 39
SexAI A'CCwGG_T
Cuts at: 897 1167 897
Size: 270 5441
SfcI C'TryA_G
Cuts at: 33 164 309 571 2754 3439 4432 4623 33
Size: 131 145 262 2183 685 993 191 1121
Fragments arranged by size:
2183 1121 993 685 262 191 145 131
SfoI GGC'GCC
Cuts at: 3143 3391 3143
Size: 248 5463
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5711
SmaI CCC'GGG
Cuts at: 2733 2733
Size: 5711
SmlI C'TyrA_G
Cuts at: 917 2866 3781 4273 4535 917
Size: 1949 915 492 262 2093
Fragments arranged by size:
2093 1949 915 492 262
SpeI A'CTAG_T
Cuts at: 67 1858 67
Size: 1791 3920
SphI G_CATG'C
Cuts at: 2764 3264 2764
Size: 500 5211
StuI AGG'CCT
Cuts at: 1320 1320
Size: 5711
StyI C'CwwG_G
Cuts at: 217 2593 2845 3199 217
Size: 2376 252 354 2729
Fragments arranged by size:
2729 2376 354 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3294 3427 3886 4095 3294
Size: 133 459 209 4910
Fragments arranged by size:
4910 459 209 133
TatI w'GTAC_w
Cuts at: 552 1018 1096 1135 1252 2435 5226 552
Size: 466 78 39 117 1183 2791 1037
Fragments arranged by size:
2791 1183 1037 466 117 78 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 191 223 5228 5260 191
Size: 32 5005 32 642
Fragments arranged by size:
5005 642 32 32
Tth111I GACn'n_nGTC
Cuts at: 1740 3508 1740
Size: 1768 3943
XbaI T'CTAG_A
Cuts at: 751 2742 751
Size: 1991 3720
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 432 3206 432
Size: 2774 2937
XhoI C'TCGA_G
Cuts at: 917 917
Size: 5711
XmaI C'CCGG_G
Cuts at: 2731 2731
Size: 5711
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PspXI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BfrBI BmgBI BmtI BsiWI BsmI
BsrGI BssHII BstZ17I ClaI DraIII EcoRV FseI FspAI
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PmlI PpuMI PshAI PsiI PspOMI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI SwaI XmnI ZraI