Restriction map for
pF1KB8511
(Circular) (Six-base) MAPSORT of: pF1KB8511.seq Check: 7191 from: 1 to: 5936
pF1KB8511 5936 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1678 1800 1678
Size: 122 5814
AccI GT'mk_AC
Cuts at: 2974 2974
Size: 5936
Acc65I G'GTAC_C
Cuts at: 2952 2952
Size: 5936
AclI AA'CG_TT
Cuts at: 5776 5776
Size: 5936
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1376 2440 2503 2629 3761 4193 4940 5208 1376
Size: 1064 63 126 1132 432 747 268 2104
Fragments arranged by size:
2104 1132 1064 747 432 268 126 63
AfeI AGC'GCT
Cuts at: 2806 2806
Size: 5936
AflIII A'CryG_T
Cuts at: 3288 4392 5369 3288
Size: 1104 977 3855
Fragments arranged by size:
3855 1104 977
AgeI A'CCGG_T
Cuts at: 4324 4324
Size: 5936
AhdI GACnn_n'nnGTC
Cuts at: 904 904
Size: 5936
AleI CACnn'nnGTG
Cuts at: 1405 2340 1405
Size: 935 5001
AlwNI CAG_nnn'CTG
Cuts at: 3241 4808 3241
Size: 1567 4369
ApaI G_GGCC'C
Cuts at: 215 2091 215
Size: 1876 4060
ApaLI G'TGCA_C
Cuts at: 1781 4706 1781
Size: 2925 3011
ApoI r'AATT_y
Cuts at: 939 2940 5289 5545 939
Size: 2001 2349 256 1330
Fragments arranged by size:
2349 2001 1330 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5936
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5936
AvaI C'yCGr_G
Cuts at: 589 1297 1874 2132 2387 2566 2956 589
Size: 708 577 258 255 179 390 3569
Fragments arranged by size:
3569 708 577 390 258 255 179
AvrII C'CTAG_G
Cuts at: 132 132
Size: 5936
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2523 2556 2523
Size: 33 5903
BamHI G'GATC_C
Cuts at: 708 1625 2961 708
Size: 917 1336 3683
Fragments arranged by size:
3683 1336 917
BanI G'GyrC_C
Cuts at: 178 1948 2952 3366 3614 3649 178
Size: 1770 1004 414 248 35 2465
Fragments arranged by size:
2465 1770 1004 414 248 35
BanII G_rGCy'C
Cuts at: 215 1415 2091 2950 3980 215
Size: 1200 676 859 1030 2171
Fragments arranged by size:
2171 1200 1030 859 676
BbeI G_GCGC'C
Cuts at: 182 1952 3370 3618 182
Size: 1770 1418 248 2500
Fragments arranged by size:
2500 1770 1418 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2368 2402 2368
Size: 34 5902
BciVI GTATCCnnnnn_n'
Cuts at: 2551 3834 4595 5936 2551
Size: 1283 761 1341 2551
Fragments arranged by size:
2551 1341 1283 761
BclI T'GATC_A
Cuts at: 3456 3456
Size: 5936
BglI GCCn_nnn'nGGC
Cuts at: 2014 2014
Size: 5936
BglII A'GATC_T
Cuts at: 2036 2193 3451 5488 2036
Size: 157 1258 2037 2484
Fragments arranged by size:
2484 2037 1258 157
BlpI GC'TnA_GC
Cuts at: 580 871 1375 1520 3048 580
Size: 291 504 145 1528 3468
Fragments arranged by size:
3468 1528 504 291 145
Bme1580I G_kGCm'C
Cuts at: 215 1126 1785 2091 2124 2530 3561 3654 4710
Size: 911 659 306 33 406 1031 93 1056
Cuts at: 4710 215
Size: 1441
Fragments arranged by size:
1441 1056 1031 911 659 406 306 93
33
BmrI ACTGGGnnnn_n'
Cuts at: 1082 3148 3229 3564 5708 1082
Size: 2066 81 335 2144 1310
Fragments arranged by size:
2144 2066 1310 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 869 4303 869
Size: 3434 2502
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1456 1546 1795 2080 2179 2383 2485 1456
Size: 90 249 285 99 204 102 4907
Fragments arranged by size:
4907 285 249 204 102 99 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 360 526 985 3112 3991 4483 4781 360
Size: 166 459 2127 879 492 298 1515
Fragments arranged by size:
2127 1515 879 492 459 298 166
BsaI GGTCTCn'nnnn_
Cuts at: 1432 5640 1432
Size: 4208 1728
BsaAI yAC'GTr
Cuts at: 159 3289 159
Size: 3130 2806
BsaBI GATnn'nnATC
Cuts at: 6 1234 3450 5487 6
Size: 1228 2216 2037 455
Fragments arranged by size:
2216 2037 1228 455
BsaHI Gr'CG_yC
Cuts at: 179 787 1949 3367 3615 4317 5813 179
Size: 608 1162 1418 248 702 1496 302
Fragments arranged by size:
1496 1418 1162 702 608 302 248
BsaWI w'CCGG_w
Cuts at: 685 2536 3125 3646 4324 4598 4745 5156 685
Size: 1851 589 521 678 274 147 411 1465
Fragments arranged by size:
1851 1465 678 589 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2827 2857 2827
Size: 30 5906
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 977 988 1726 2047 2218 2278 2380 2598 2794
Size: 11 738 321 171 60 102 218 196
Cuts at: 2794 2806 977
Size: 12 4107
Fragments arranged by size:
4107 738 321 218 196 171 102 60
12 11
BseYI C'CCAG_C
Cuts at: 168 361 1654 1819 3361 4119 4696 5192 168
Size: 193 1293 165 1542 758 577 496 912
Fragments arranged by size:
1542 1293 912 758 577 496 193 165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2837 2837
Size: 5936
BsiEI CG_ry'CG
Cuts at: 104 1572 2913 3478 3524 4732 104
Size: 1468 1341 565 46 1208 1308
Fragments arranged by size:
1468 1341 1308 1208 565 46
BsiHKAI G_wGCw'C
Cuts at: 1415 1785 2394 2639 2950 3728 3918 4710 1415
Size: 370 609 245 311 778 190 792 2641
Fragments arranged by size:
2641 792 778 609 370 311 245 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1009 1009
Size: 5936
Bsp1286I G_dGCh'C
Cuts at: 215 1126 1415 1785 2091 2124 2394 2530 2639
Size: 911 289 370 306 33 270 136 109
Cuts at: 2639 2950 3561 3654 3728 3918 3980 4710 215
Size: 311 611 93 74 190 62 730 1441
Fragments arranged by size:
1441 911 730 611 370 311 306 289
270 190 136 109 93 74 62 33
BspEI T'CCGG_A
Cuts at: 5156 5156
Size: 5936
BspHI T'CATG_A
Cuts at: 1103 2141 5112 1103
Size: 1038 2971 1927
Fragments arranged by size:
2971 1927 1038
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1678 1800 2986 3200 3502 3883 1678
Size: 122 1186 214 302 381 3731
Fragments arranged by size:
3731 1186 381 302 214 122
BsrBI CCG'CTC
Cuts at: 50 2085 4228 4282 5766 5931 50
Size: 2035 2143 54 1484 165 55
Fragments arranged by size:
2143 2035 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3848 3848
Size: 5936
BsrFI r'CCGG_y
Cuts at: 2908 3934 4115 4324 2908
Size: 1026 181 209 4520
Fragments arranged by size:
4520 1026 209 181
BssHII G'CGCG_C
Cuts at: 1842 1842
Size: 5936
BssSI C'ACGA_G
Cuts at: 1784 4207 4565 1784
Size: 2423 358 3155
Fragments arranged by size:
3155 2423 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3313 3313
Size: 5936
BstBI TT'CG_AA
Cuts at: 4297 4297
Size: 5936
BstXI CCAn_nnnn'nTGG
Cuts at: 1188 1407 1602 1965 2598 1188
Size: 219 195 363 633 4526
Fragments arranged by size:
4526 633 363 219 195
BstYI r'GATC_y
Cuts at: 1 321 708 1625 2036 2193 2961 3428 3451
Size: 320 387 917 411 157 768 467 23
Cuts at: 3451 3786 4032 5044 5130 5142 5488 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 917 768 467 449 411 387 346
335 320 246 157 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2896 2896
Size: 5936
BtgI C'CryG_G
Cuts at: 1187 1866 2645 1187
Size: 679 779 4478
Fragments arranged by size:
4478 779 679
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 614 2475 2847 3886 4067 4230 614
Size: 1861 372 1039 181 163 2320
Fragments arranged by size:
2320 1861 1039 372 181 163
BtsI GCAGTG_nn'
Cuts at: 1627 1914 2124 3179 1627
Size: 287 210 1055 4384
Fragments arranged by size:
4384 1055 287 210
DraI TTT'AAA
Cuts at: 938 2935 938
Size: 1997 3939
DrdI GACnn_nn'nnGTC
Cuts at: 3642 4500 3642
Size: 858 5078
EaeI y'GGCC_r
Cuts at: 837 1664 2102 2507 2642 2648 2906 2910 3521
Size: 827 438 405 135 6 258 4 611
Cuts at: 3521 3695 4086 4113 5458 837
Size: 174 391 27 1345 1315
Fragments arranged by size:
1345 1315 827 611 438 405 391 258
174 135 27 6 4
EagI C'GGCC_G
Cuts at: 2910 3521 2910
Size: 611 5325
EarI CTCTTCn'nnn_
Cuts at: 376 566 1822 3959 4169 376
Size: 190 1256 2137 210 2143
Fragments arranged by size:
2143 2137 1256 210 190
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4144 4454 4600 5746 4144
Size: 310 146 1146 4334
Fragments arranged by size:
4334 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1413 2948 1413
Size: 1535 4401
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1376 1456 1546 1795 2080 2179 2383 2440 2485
Size: 80 90 249 285 99 204 57 45
Cuts at: 2485 2503 2629 3761 4193 4940 5208 1376
Size: 18 126 1132 432 747 268 2104
Fragments arranged by size:
2104 1132 747 432 285 268 249 204
126 99 90 80 57 45 18
EcoNI CCTnn'n_nnAGG
Cuts at: 809 809
Size: 5936
EcoO109I rG'GnC_Cy
Cuts at: 2208 2576 3075 2208
Size: 368 499 5069
Fragments arranged by size:
5069 499 368
EcoRI G'AATT_C
Cuts at: 2940 2940
Size: 5936
EcoRV GAT'ATC
Cuts at: 978 978
Size: 5936
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2943 3955 2943
Size: 1012 4924
FseI GG_CCGG'CC
Cuts at: 2912 2912
Size: 5936
FspI TGC'GCA
Cuts at: 3717 3717
Size: 5936
HaeII r_GCGC'y
Cuts at: 182 1952 2518 2808 3370 3618 4640 5619 182
Size: 1770 566 290 562 248 1022 979 499
Fragments arranged by size:
1770 1022 979 566 562 499 290 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 890 922 953 983 985 1008 1015 1040 3778
Size: 32 31 30 2 23 7 25 2738
Cuts at: 3778 3808 3810 3840 890
Size: 30 2 30 2986
Fragments arranged by size:
2986 2738 32 31 30 30 30 25
23 7 2 2
HincII GTy'rAC
Cuts at: 912 2975 912
Size: 2063 3873
KasI G'GCGC_C
Cuts at: 178 1948 3366 3614 178
Size: 1770 1418 248 2500
Fragments arranged by size:
2500 1770 1418 248
KpnI G_GTAC'C
Cuts at: 2956 2956
Size: 5936
MluI A'CGCG_T
Cuts at: 5369 5369
Size: 5936
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 546 2429 4607 4791 546
Size: 1883 2178 184 1691
Fragments arranged by size:
2178 1883 1691 184
MscI TGG'CCA
Cuts at: 839 1666 2104 2509 2650 3697 839
Size: 827 438 405 141 1047 3078
Fragments arranged by size:
3078 1047 827 438 405 141
MslI CAynn'nnrTG
Cuts at: 856 1405 1549 2340 3925 4052 856
Size: 549 144 791 1585 127 2740
Fragments arranged by size:
2740 1585 791 549 144 127
MspA1I CmG'CkG
Cuts at: 621 2670 3047 3361 3721 4734 4979 621
Size: 2049 377 314 360 1013 245 1578
Fragments arranged by size:
2049 1578 1013 377 360 314 245
NaeI GCC'GGC
Cuts at: 2910 4117 2910
Size: 1207 4729
NarI GG'CG_CC
Cuts at: 179 1949 3367 3615 179
Size: 1770 1418 248 2500
Fragments arranged by size:
2500 1770 1418 248
NcoI C'CATG_G
Cuts at: 1866 1866
Size: 5936
NgoMIV G'CCGG_C
Cuts at: 2908 4115 2908
Size: 1207 4729
NspI r_CATG'y
Cuts at: 1306 2989 3489 4396 1306
Size: 1683 500 907 2846
Fragments arranged by size:
2846 1683 907 500
PasI CC'CwG_GG
Cuts at: 2437 2437
Size: 5936
PciI A'CATG_T
Cuts at: 4392 4392
Size: 5936
PflMI CCAn_nnn'nTGG
Cuts at: 2240 2693 2240
Size: 453 5483
PfoI T'CCnGG_A
Cuts at: 1628 1628
Size: 5936
PmeI GTTT'AAAC
Cuts at: 2935 2935
Size: 5936
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 685 717 5075 5107 685
Size: 32 4358 32 1514
Fragments arranged by size:
4358 1514 32 32
PspOMI G'GGCC_C
Cuts at: 211 2087 211
Size: 1876 4060
PspXI vC'TCGA_Gb
Cuts at: 1297 2387 2566 1297
Size: 1090 179 4667
Fragments arranged by size:
4667 1090 179
PstI C_TGCA'G
Cuts at: 1811 1901 1986 2522 2768 2983 3668 1811
Size: 90 85 536 246 215 685 4079
Fragments arranged by size:
4079 685 536 246 215 90 85
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5936
PvuII CAG'CTG
Cuts at: 3361 3721 3361
Size: 360 5576
RsrII CG'GwC_CG
Cuts at: 4131 4131
Size: 5936
SacI G_AGCT'C
Cuts at: 1415 2950 1415
Size: 1535 4401
SalI G'TCGA_C
Cuts at: 2973 2973
Size: 5936
SapI GCTCTTCn'nnn_
Cuts at: 3959 4169 3959
Size: 210 5726
SbfI CC_TGCA'GG
Cuts at: 1811 2983 1811
Size: 1172 4764
ScaI AGT'ACT
Cuts at: 5453 5453
Size: 5936
SfcI C'TryA_G
Cuts at: 33 1490 1807 1897 1982 2518 2764 2979 3664
Size: 1457 317 90 85 536 246 215 685
Cuts at: 3664 4657 4848 33
Size: 993 191 1121
Fragments arranged by size:
1457 1121 993 685 536 317 246 215
191 90 85
SfiI GGCCn_nnn'nGGCC
Cuts at: 2014 2014
Size: 5936
SfoI GGC'GCC
Cuts at: 180 1950 3368 3616 180
Size: 1770 1418 248 2500
Fragments arranged by size:
2500 1770 1418 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5936
SmaI CCC'GGG
Cuts at: 2134 2958 2134
Size: 824 5112
SmlI C'TyrA_G
Cuts at: 375 541 1000 1297 2387 2566 3091 4006 4498
Size: 166 459 297 1090 179 525 915 492
Cuts at: 4498 4760 375
Size: 262 1551
Fragments arranged by size:
1551 1090 915 525 492 459 297 262
179 166
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5936
SphI G_CATG'C
Cuts at: 2989 3489 2989
Size: 500 5436
StyI C'CwwG_G
Cuts at: 132 1181 1866 2105 2510 2681 2855 3070 3424
Size: 1049 685 239 405 171 174 215 354
Cuts at: 3424 132
Size: 2644
Fragments arranged by size:
2644 1049 685 405 354 239 215 174
171
SwaI ATTT'AAAT
Cuts at: 938 938
Size: 5936
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1511 1586 2154 3519 3652 4111 4320 1511
Size: 75 568 1365 133 459 209 3127
Fragments arranged by size:
3127 1365 568 459 209 133 75
TatI w'GTAC_w
Cuts at: 5451 5451
Size: 5936
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 732 764 2793 2825 2827 2859 5453 5485 732
Size: 32 2029 32 2 32 2594 32 1183
Fragments arranged by size:
2594 2029 1183 32 32 32 32 2
Tth111I GACn'n_nGTC
Cuts at: 3733 3733
Size: 5936
XbaI T'CTAG_A
Cuts at: 2967 2967
Size: 5936
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3431 3431
Size: 5936
XhoI C'TCGA_G
Cuts at: 1297 2387 2566 1297
Size: 1090 179 4667
Fragments arranged by size:
4667 1090 179
XmaI C'CCGG_G
Cuts at: 2132 2956 2132
Size: 824 5112
XmnI GAAnn'nnTTC
Cuts at: 127 2926 127
Size: 2799 3137
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AflII AlfI AloI AscI BbsI BbvCI BfrBI
BmgBI BmtI Bpu10I BsiWI BsmI BsrGI BstEII BstZ17I
ClaI CspCI DraIII FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PmlI PpuMI PshAI
PsiI PsrI SacII SanDI SexAI SgrAI SnaBI SrfI
SspI StuI ZraI