Restriction map for
pF1KB8515
(Circular) (Six-base) MAPSORT of: pF1KB8515.seq Check: 178 from: 1 to: 5957
pF1KB8515 5957 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 490 490
Size: 5957
AatII G_ACGT'C
Cuts at: 1342 2828 1342
Size: 1486 4471
AccI GT'mk_AC
Cuts at: 2336 2995 2336
Size: 659 5298
Acc65I G'GTAC_C
Cuts at: 2973 2973
Size: 5957
AclI AA'CG_TT
Cuts at: 5797 5797
Size: 5957
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 301 562 1008 1486 2083 2368 2730 3782
Size: 6 261 446 478 597 285 362 1052
Cuts at: 3782 4214 4961 5229 295
Size: 432 747 268 1023
Fragments arranged by size:
1052 1023 747 597 478 446 432 362
285 268 261 6
AfeI AGC'GCT
Cuts at: 846 846
Size: 5957
AflII C'TTAA_G
Cuts at: 1309 1309
Size: 5957
AflIII A'CryG_T
Cuts at: 2195 2255 2492 3309 4413 5390 2195
Size: 60 237 817 1104 977 2762
Fragments arranged by size:
2762 1104 977 817 237 60
AgeI A'CCGG_T
Cuts at: 4345 4345
Size: 5957
AleI CACnn'nnGTG
Cuts at: 1327 1669 1327
Size: 342 5615
AlwNI CAG_nnn'CTG
Cuts at: 141 228 396 405 915 3262 4829 141
Size: 87 168 9 510 2347 1567 1269
Fragments arranged by size:
2347 1567 1269 510 168 87 9
ApaI G_GGCC'C
Cuts at: 340 1046 340
Size: 706 5251
ApaLI G'TGCA_C
Cuts at: 4727 4727
Size: 5957
ApoI r'AATT_y
Cuts at: 1459 1601 2023 2961 5310 5566 1459
Size: 142 422 938 2349 256 1850
Fragments arranged by size:
2349 1850 938 422 256 142
AseI AT'TA_AT
Cuts at: 20 20
Size: 5957
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5957
AvaI C'yCGr_G
Cuts at: 211 1220 1885 2084 2977 211
Size: 1009 665 199 893 3191
Fragments arranged by size:
3191 1009 893 665 199
BamHI G'GATC_C
Cuts at: 1741 1830 2982 1741
Size: 89 1152 4716
Fragments arranged by size:
4716 1152 89
BanI G'GyrC_C
Cuts at: 782 817 1714 2780 2973 3387 3635 3670 782
Size: 35 897 1066 193 414 248 35 3069
Fragments arranged by size:
3069 1066 897 414 248 193 35 35
BanII G_rGCy'C
Cuts at: 340 364 1001 1046 1825 1861 2270 2875 2971
Size: 24 637 45 779 36 409 605 96
Cuts at: 2971 4001 340
Size: 1030 2296
Fragments arranged by size:
2296 1030 779 637 605 409 96 45
36 24
BbeI G_GCGC'C
Cuts at: 2784 3391 3639 2784
Size: 607 248 5102
Fragments arranged by size:
5102 607 248
BbsI GAAGACnn'nnnn_
Cuts at: 2586 2586
Size: 5957
BbvCI CC'TCA_GC
Cuts at: 1541 1541
Size: 5957
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2698 2732 2698
Size: 34 5923
BciVI GTATCCnnnnn_n'
Cuts at: 3855 4616 5957 3855
Size: 761 1341 3855
Fragments arranged by size:
3855 1341 761
BclI T'GATC_A
Cuts at: 3477 3477
Size: 5957
BglI GCCn_nnn'nGGC
Cuts at: 1253 2056 2773 1253
Size: 803 717 4437
Fragments arranged by size:
4437 803 717
BglII A'GATC_T
Cuts at: 3472 5509 3472
Size: 2037 3920
BlpI GC'TnA_GC
Cuts at: 841 3069 841
Size: 2228 3729
Bme1580I G_kGCm'C
Cuts at: 340 1046 1404 1455 1711 2804 3582 3675 4731
Size: 706 358 51 256 1093 778 93 1056
Cuts at: 4731 340
Size: 1566
Fragments arranged by size:
1566 1093 1056 778 706 358 256 93
51
BmgBI CAC'GTC
Cuts at: 2256 2495 2256
Size: 239 5718
BmrI ACTGGGnnnn_n'
Cuts at: 450 2027 2536 3169 3250 3585 5729 450
Size: 1577 509 633 81 335 2144 678
Fragments arranged by size:
2144 1577 678 633 509 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 170 1015 1400 1595 4324 170
Size: 845 385 195 2729 1803
Fragments arranged by size:
2729 1803 845 385 195
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 250 256 538 724 958 1123 1408 1432
Size: 87 6 282 186 234 165 285 24
Cuts at: 1432 1721 163
Size: 289 4399
Fragments arranged by size:
4399 289 285 282 234 186 165 87
24 6
Bpu10I CC'TnA_GC
Cuts at: 499 1207 1541 1855 499
Size: 708 334 314 4601
Fragments arranged by size:
4601 708 334 314
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3133 4012 4504 4802 3133
Size: 879 492 298 4288
Fragments arranged by size:
4288 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1121 1132 1894 1903 5661 1121
Size: 11 762 9 3758 1417
Fragments arranged by size:
3758 1417 762 11 9
BsaAI yAC'GTr
Cuts at: 417 1667 2196 3310 417
Size: 1250 529 1114 3064
Fragments arranged by size:
3064 1250 1114 529
BsaBI GATnn'nnATC
Cuts at: 6 658 1651 3471 5508 6
Size: 652 993 1820 2037 455
Fragments arranged by size:
2037 1820 993 652 455
BsaHI Gr'CG_yC
Cuts at: 1339 2781 2825 3388 3636 4338 5834 1339
Size: 1442 44 563 248 702 1496 1462
Fragments arranged by size:
1496 1462 1442 702 563 248 44
BsaWI w'CCGG_w
Cuts at: 602 1682 2187 2435 3146 3667 4345 4619 4766
Size: 1080 505 248 711 521 678 274 147
Cuts at: 4766 5177 602
Size: 411 1382
Fragments arranged by size:
1382 1080 711 678 521 505 411 274
248 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 511 541 2182 2212 511
Size: 30 1641 30 4256
Fragments arranged by size:
4256 1641 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 354 1002 2005 2599 2602 2857 354
Size: 648 1003 594 3 255 3454
Fragments arranged by size:
3454 1003 648 594 255 3
BseYI C'CCAG_C
Cuts at: 180 623 915 1215 1986 2301 2697 2759 3382
Size: 443 292 300 771 315 396 62 623
Cuts at: 3382 4140 4717 5213 180
Size: 758 577 496 924
Fragments arranged by size:
924 771 758 623 577 496 443 396
315 300 292 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 391 1525 1950 2224 370
Size: 21 1134 425 274 4103
Fragments arranged by size:
4103 1134 425 274 21
BsiEI CG_ry'CG
Cuts at: 104 2222 2645 2817 3499 3545 4753 104
Size: 2118 423 172 682 46 1208 1308
Fragments arranged by size:
2118 1308 1208 682 423 172 46
BsiHKAI G_wGCw'C
Cuts at: 803 1001 1825 1861 2015 2195 2971 3749 3939
Size: 198 824 36 154 180 776 778 190
Cuts at: 3939 4731 803
Size: 792 2029
Fragments arranged by size:
2029 824 792 778 776 198 190 180
154 36
BsmBI CGTCTCn'nnnn_
Cuts at: 2081 2081
Size: 5957
Bsp1286I G_dGCh'C
Cuts at: 340 364 803 1001 1046 1404 1455 1711 1825
Size: 24 439 198 45 358 51 256 114
Cuts at: 1825 1861 2015 2195 2270 2804 2875 2971 3582
Size: 36 154 180 75 534 71 96 611
Cuts at: 3582 3675 3749 3939 4001 4731 340
Size: 93 74 190 62 730 1566
Fragments arranged by size:
1566 730 611 534 439 358 256 198
190 180 154 114 96 93 75 74
71 62 51 45 36 24
BspEI T'CCGG_A
Cuts at: 1682 5177 1682
Size: 3495 2462
BspHI T'CATG_A
Cuts at: 2660 5133 2660
Size: 2473 3484
BspMI ACCTGCnnnn'nnnn_
Cuts at: 308 389 490 3007 3221 3523 3904 308
Size: 81 101 2517 214 302 381 2361
Fragments arranged by size:
2517 2361 381 302 214 101 81
BsrBI CCG'CTC
Cuts at: 50 240 246 942 1089 2691 4249 4303 5787
Size: 190 6 696 147 1602 1558 54 1484
Cuts at: 5787 5952 50
Size: 165 55
Fragments arranged by size:
1602 1558 1484 696 190 165 147 55
54 6
BsrDI GCAATG_nn'
Cuts at: 591 3869 591
Size: 3278 2679
BsrFI r'CCGG_y
Cuts at: 694 2221 3955 4136 4345 694
Size: 1527 1734 181 209 2306
Fragments arranged by size:
2306 1734 1527 209 181
BssSI C'ACGA_G
Cuts at: 4228 4586 4228
Size: 358 5599
BstAPI GCAn_nnn'nTGC
Cuts at: 405 633 1073 1503 3334 405
Size: 228 440 430 1831 3028
Fragments arranged by size:
3028 1831 440 430 228
BstBI TT'CG_AA
Cuts at: 4318 4318
Size: 5957
BstEII G'GTnAC_C
Cuts at: 1545 1545
Size: 5957
BstXI CCAn_nnnn'nTGG
Cuts at: 321 463 321
Size: 142 5815
BstYI r'GATC_y
Cuts at: 1 1741 1830 2701 2982 3449 3472 3807 4053
Size: 1740 89 871 281 467 23 335 246
Cuts at: 4053 5065 5151 5163 5509 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1740 1012 871 467 449 346 335 281
246 89 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2477 2477
Size: 5957
BtgI C'CryG_G
Cuts at: 107 1147 1703 1757 2903 107
Size: 1040 556 54 1146 3161
Fragments arranged by size:
3161 1146 1040 556 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3907 4088 4251 3907
Size: 181 163 5613
Fragments arranged by size:
5613 181 163
BtsI GCAGTG_nn'
Cuts at: 1877 2138 3200 1877
Size: 261 1062 4634
Fragments arranged by size:
4634 1062 261
ClaI AT'CG_AT
Cuts at: 1655 1655
Size: 5957
DraI TTT'AAA
Cuts at: 2956 2956
Size: 5957
DraIII CAC_nnn'GTG
Cuts at: 420 807 1073 420
Size: 387 266 5304
Fragments arranged by size:
5304 387 266
DrdI GACnn_nn'nnGTC
Cuts at: 2493 2605 3663 4521 2493
Size: 112 1058 858 3929
Fragments arranged by size:
3929 1058 858 112
EaeI y'GGCC_r
Cuts at: 188 437 488 530 696 809 1037 1254 2219
Size: 249 51 42 166 113 228 217 965
Cuts at: 2219 2642 2814 2939 3542 3716 4107 4134 5479
Size: 423 172 125 603 174 391 27 1345
Cuts at: 5479 188
Size: 666
Fragments arranged by size:
1345 965 666 603 423 391 249 228
217 174 172 166 125 113 51 42
27
EagI C'GGCC_G
Cuts at: 2219 2642 2814 3542 2219
Size: 423 172 728 4634
Fragments arranged by size:
4634 728 423 172
EarI CTCTTCn'nnn_
Cuts at: 767 989 1813 2173 3980 4190 767
Size: 222 824 360 1807 210 2534
Fragments arranged by size:
2534 1807 824 360 222 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 574 667 2277 4165 4475 4621 5767 127
Size: 447 93 1610 1888 310 146 1146 317
Fragments arranged by size:
1888 1610 1146 447 317 310 146 93
EcoICRI GAG'CTC
Cuts at: 999 1823 1859 2969 999
Size: 824 36 1110 3987
Fragments arranged by size:
3987 1110 824 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 250 256 295 301 538 562 724 958
Size: 87 6 39 6 237 24 162 234
Cuts at: 958 1008 1123 1408 1432 1486 1721 2083 2368
Size: 50 115 285 24 54 235 362 285
Cuts at: 2368 2730 3782 4214 4961 5229 163
Size: 362 1052 432 747 268 891
Fragments arranged by size:
1052 891 747 432 362 362 285 285
268 237 235 234 162 115 87 54
50 39 24 24 6 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2410 2745 2410
Size: 335 5622
EcoO109I rG'GnC_Cy
Cuts at: 337 855 1043 1097 1174 1181 1224 1443 1918
Size: 518 188 54 77 7 43 219 475
Cuts at: 1918 2229 2343 2456 2859 3096 337
Size: 311 114 113 403 237 3198
Fragments arranged by size:
3198 518 475 403 311 237 219 188
114 113 77 54 43 7
EcoRI G'AATT_C
Cuts at: 2023 2961 2023
Size: 938 5019
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3976 3976
Size: 5957
FseI GG_CCGG'CC
Cuts at: 698 698
Size: 5957
FspI TGC'GCA
Cuts at: 766 3738 766
Size: 2972 2985
HaeII r_GCGC'y
Cuts at: 688 848 2784 3391 3639 4661 5640 688
Size: 160 1936 607 248 1022 979 1005
Fragments arranged by size:
1936 1022 1005 979 607 248 160
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2245 2277 3799 3829 3831 3861 2245
Size: 32 1522 30 2 30 4341
Fragments arranged by size:
4341 1522 32 30 30 2
HincII GTy'rAC
Cuts at: 2610 2996 2610
Size: 386 5571
KasI G'GCGC_C
Cuts at: 2780 3387 3635 2780
Size: 607 248 5102
Fragments arranged by size:
5102 607 248
KpnI G_GTAC'C
Cuts at: 2977 2977
Size: 5957
MluI A'CGCG_T
Cuts at: 5390 5390
Size: 5957
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 929 1451 1611 2333 4628 4812 929
Size: 522 160 722 2295 184 2074
Fragments arranged by size:
2295 2074 722 522 184 160
MscI TGG'CCA
Cuts at: 532 3718 532
Size: 3186 2771
MslI CAynn'nnrTG
Cuts at: 667 1327 1669 1771 3946 4073 667
Size: 660 342 102 2175 127 2551
Fragments arranged by size:
2551 2175 660 342 127 102
MspA1I CmG'CkG
Cuts at: 161 180 228 396 702 1018 1440 1509 2905
Size: 19 48 168 306 316 422 69 1396
Cuts at: 2905 3068 3382 3742 4755 5000 161
Size: 163 314 360 1013 245 1118
Fragments arranged by size:
1396 1118 1013 422 360 316 314 306
245 168 163 69 48 19
NaeI GCC'GGC
Cuts at: 696 4138 696
Size: 3442 2515
NarI GG'CG_CC
Cuts at: 2781 3388 3636 2781
Size: 607 248 5102
Fragments arranged by size:
5102 607 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5957
NgoMIV G'CCGG_C
Cuts at: 694 4136 694
Size: 3442 2515
NspI r_CATG'y
Cuts at: 672 3010 3510 4417 672
Size: 2338 500 907 2212
Fragments arranged by size:
2338 2212 907 500
PasI CC'CwG_GG
Cuts at: 1524 1726 2572 1524
Size: 202 846 4909
Fragments arranged by size:
4909 846 202
PciI A'CATG_T
Cuts at: 4413 4413
Size: 5957
PflMI CCAn_nnn'nTGG
Cuts at: 915 1148 1367 915
Size: 233 219 5505
Fragments arranged by size:
5505 233 219
PfoI T'CCnGG_A
Cuts at: 1737 1868 2366 2921 1737
Size: 131 498 555 4773
Fragments arranged by size:
4773 555 498 131
PmeI GTTT'AAAC
Cuts at: 2956 2956
Size: 5957
PmlI CAC'GTG
Cuts at: 417 1667 2196 417
Size: 1250 529 4178
Fragments arranged by size:
4178 1250 529
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5096 5128 5096
Size: 32 5925
PpuMI rG'GwC_Cy
Cuts at: 1097 1174 1181 2229 2343 2456 1097
Size: 77 7 1048 114 113 4598
Fragments arranged by size:
4598 1048 114 113 77 7
PspOMI G'GGCC_C
Cuts at: 336 1042 336
Size: 706 5251
PstI C_TGCA'G
Cuts at: 401 692 1126 3004 3689 401
Size: 291 434 1878 685 2669
Fragments arranged by size:
2669 1878 685 434 291
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5957
PvuII CAG'CTG
Cuts at: 161 180 228 396 702 1509 3382 3742 161
Size: 19 48 168 306 807 1873 360 2376
Fragments arranged by size:
2376 1873 807 360 306 168 48 19
RsrII CG'GwC_CG
Cuts at: 861 4152 861
Size: 3291 2666
SacI G_AGCT'C
Cuts at: 1001 1825 1861 2971 1001
Size: 824 36 1110 3987
Fragments arranged by size:
3987 1110 824 36
SacII CC_GC'GG
Cuts at: 2906 2906
Size: 5957
SalI G'TCGA_C
Cuts at: 2994 2994
Size: 5957
SanDI GG'GwC_CC
Cuts at: 1097 1181 2456 1097
Size: 84 1275 4598
Fragments arranged by size:
4598 1275 84
SapI GCTCTTCn'nnn_
Cuts at: 767 989 1813 3980 4190 767
Size: 222 824 2167 210 2534
Fragments arranged by size:
2534 2167 824 222 210
SbfI CC_TGCA'GG
Cuts at: 3004 3004
Size: 5957
ScaI AGT'ACT
Cuts at: 2215 5474 2215
Size: 3259 2698
SfcI C'TryA_G
Cuts at: 33 397 579 688 1062 1122 2308 2337 3000
Size: 364 182 109 374 60 1186 29 663
Cuts at: 3000 3685 4678 4869 33
Size: 685 993 191 1121
Fragments arranged by size:
1186 1121 993 685 663 374 364 191
182 109 60 29
SfoI GGC'GCC
Cuts at: 2782 3389 3637 2782
Size: 607 248 5102
Fragments arranged by size:
5102 607 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5957
SmaI CCC'GGG
Cuts at: 213 1222 2979 213
Size: 1009 1757 3191
Fragments arranged by size:
3191 1757 1009
SmlI C'TyrA_G
Cuts at: 1309 3112 4027 4519 4781 1309
Size: 1803 915 492 262 2485
Fragments arranged by size:
2485 1803 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5957
SphI G_CATG'C
Cuts at: 672 3010 3510 672
Size: 2338 500 3119
Fragments arranged by size:
3119 2338 500
StyI C'CwwG_G
Cuts at: 107 596 732 851 974 3091 3445 107
Size: 489 136 119 123 2117 354 2619
Fragments arranged by size:
2619 2117 489 354 136 123 119
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 457 745 1659 2518 3540 3673 4132 4341 457
Size: 288 914 859 1022 133 459 209 2073
Fragments arranged by size:
2073 1022 914 859 459 288 209 133
TatI w'GTAC_w
Cuts at: 2213 5472 2213
Size: 3259 2698
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1338 1370 1746 1778 2182 2214 2516 2548 5474
Size: 32 376 32 404 32 302 32 2926
Cuts at: 5474 5506 1338
Size: 32 1789
Fragments arranged by size:
2926 1789 404 376 302 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 3754 3754
Size: 5957
XbaI T'CTAG_A
Cuts at: 2988 2988
Size: 5957
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 318 912 3452 318
Size: 594 2540 2823
Fragments arranged by size:
2823 2540 594
XmaI C'CCGG_G
Cuts at: 211 1220 2977 211
Size: 1009 1757 3191
Fragments arranged by size:
3191 1757 1009
XmnI GAAnn'nnTTC
Cuts at: 1603 1627 2167 1603
Size: 24 540 5393
Fragments arranged by size:
5393 540 24
ZraI GAC'GTC
Cuts at: 1340 2826 1340
Size: 1486 4471
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AhdI AlfI AloI AscI AvrII BaeI BfrBI BmtI
BsiWI BsmI BsrGI BssHII BstZ17I CspCI EcoRV FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PshAI PsiI PspXI PsrI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI