Restriction map for
pF1KB8521
(Circular) (Six-base) MAPSORT of: pF1KB8521.seq Check: 9409 from: 1 to: 5981
pF1KB8521 5981 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2565 2612 2565
Size: 47 5934
AccI GT'mk_AC
Cuts at: 3019 3019
Size: 5981
Acc65I G'GTAC_C
Cuts at: 1822 2024 2997 1822
Size: 202 973 4806
Fragments arranged by size:
4806 973 202
AclI AA'CG_TT
Cuts at: 5821 5821
Size: 5981
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 542 2116 2230 2532 2734 2810 3806 4238 4985
Size: 1574 114 302 202 76 996 432 747
Cuts at: 4985 5253 542
Size: 268 1270
Fragments arranged by size:
1574 1270 996 747 432 302 268 202
114 76
AfeI AGC'GCT
Cuts at: 2137 2137
Size: 5981
AflII C'TTAA_G
Cuts at: 1249 1249
Size: 5981
AflIII A'CryG_T
Cuts at: 263 3333 4437 5414 263
Size: 3070 1104 977 830
Fragments arranged by size:
3070 1104 977 830
AgeI A'CCGG_T
Cuts at: 4369 4369
Size: 5981
AlwNI CAG_nnn'CTG
Cuts at: 1659 2094 2192 2313 2325 3286 4853 1659
Size: 435 98 121 12 961 1567 2787
Fragments arranged by size:
2787 1567 961 435 121 98 12
ApaI G_GGCC'C
Cuts at: 1872 2690 1872
Size: 818 5163
ApaLI G'TGCA_C
Cuts at: 2566 4751 2566
Size: 2185 3796
ApoI r'AATT_y
Cuts at: 516 1078 2985 5334 5590 516
Size: 562 1907 2349 256 907
Fragments arranged by size:
2349 1907 907 562 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5981
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5981
AvaI C'yCGr_G
Cuts at: 747 994 1753 3001 747
Size: 247 759 1248 3727
Fragments arranged by size:
3727 1248 759 247
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 272 305 1014 1047 1806 1839 272
Size: 33 709 33 759 33 4414
Fragments arranged by size:
4414 759 709 33 33 33
BamHI G'GATC_C
Cuts at: 3006 3006
Size: 5981
BanI G'GyrC_C
Cuts at: 132 139 816 1822 2024 2640 2997 3411 3659
Size: 7 677 1006 202 616 357 414 248
Cuts at: 3659 3694 132
Size: 35 2419
Fragments arranged by size:
2419 1006 677 616 414 357 248 202
35 7
BanII G_rGCy'C
Cuts at: 973 1872 2434 2690 2962 2995 4025 973
Size: 899 562 256 272 33 1030 2929
Fragments arranged by size:
2929 1030 899 562 272 256 33
BbeI G_GCGC'C
Cuts at: 136 143 3415 3663 136
Size: 7 3272 248 2454
Fragments arranged by size:
3272 2454 248 7
BbsI GAAGACnn'nnnn_
Cuts at: 569 947 1843 2798 569
Size: 378 896 955 3752
Fragments arranged by size:
3752 955 896 378
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5947
BciVI GTATCCnnnnn_n'
Cuts at: 3879 4640 5981 3879
Size: 761 1341 3879
Fragments arranged by size:
3879 1341 761
BclI T'GATC_A
Cuts at: 3501 3501
Size: 5981
BglI GCCn_nnn'nGGC
Cuts at: 999 1204 1354 999
Size: 205 150 5626
Fragments arranged by size:
5626 205 150
BglII A'GATC_T
Cuts at: 3496 5533 3496
Size: 2037 3944
BlpI GC'TnA_GC
Cuts at: 2404 3093 2404
Size: 689 5292
Bme1580I G_kGCm'C
Cuts at: 760 1872 2018 2570 2645 2690 2701 3606 3699
Size: 1112 146 552 75 45 11 905 93
Cuts at: 3699 4755 760
Size: 1056 1986
Fragments arranged by size:
1986 1112 1056 905 552 146 93 75
45 11
BmrI ACTGGGnnnn_n'
Cuts at: 578 1827 2974 3193 3274 3609 5753 578
Size: 1249 1147 219 81 335 2144 806
Fragments arranged by size:
2144 1249 1147 806 335 219 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4348 4348
Size: 5981
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 142 519 644 1372 1588 2263 133
Size: 9 377 125 728 216 675 3851
Fragments arranged by size:
3851 728 675 377 216 125 9
Bpu10I CC'TnA_GC
Cuts at: 967 2126 967
Size: 1159 4822
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1135 1300 1384 2485 3157 4036 4528 4826 1135
Size: 165 84 1101 672 879 492 298 2290
Fragments arranged by size:
2290 1101 879 672 492 298 165 84
BsaI GGTCTCn'nnnn_
Cuts at: 622 846 1853 2239 5685 622
Size: 224 1007 386 3446 918
Fragments arranged by size:
3446 1007 918 386 224
BsaAI yAC'GTr
Cuts at: 1159 2565 3334 1159
Size: 1406 769 3806
Fragments arranged by size:
3806 1406 769
BsaBI GATnn'nnATC
Cuts at: 6 3495 5532 6
Size: 3489 2037 455
Fragments arranged by size:
3489 2037 455
BsaHI Gr'CG_yC
Cuts at: 133 140 1554 2272 3412 3660 4362 5858 133
Size: 7 1414 718 1140 248 702 1496 256
Fragments arranged by size:
1496 1414 1140 718 702 256 248 7
BsaWI w'CCGG_w
Cuts at: 1666 2905 3170 3691 4369 4643 4790 5201 1666
Size: 1239 265 521 678 274 147 411 2446
Fragments arranged by size:
2446 1239 678 521 411 274 265 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 109 139 1930 1960 109
Size: 30 1791 30 4130
Fragments arranged by size:
4130 1791 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 130 673 676 705 1207 1228 1336 1591 1773
Size: 543 3 29 502 21 108 255 182
Cuts at: 1773 1927 1942 2068 2278 2310 2836 2839 2848
Size: 154 15 126 210 32 526 3 9
Cuts at: 2848 2851 130
Size: 3 3260
Fragments arranged by size:
3260 543 526 502 255 210 182 154
126 108 32 29 21 15 9 3
3 3
BseYI C'CCAG_C
Cuts at: 790 2318 2464 2689 2911 3406 4164 4741 5237
Size: 1528 146 225 222 495 758 577 496
Cuts at: 5237 790
Size: 1534
Fragments arranged by size:
1534 1528 758 577 496 495 225 222
146
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 704 1181 2320 2434 704
Size: 477 1139 114 4251
Fragments arranged by size:
4251 1139 477 114
BsiEI CG_ry'CG
Cuts at: 104 3523 3569 4777 104
Size: 3419 46 1208 1308
Fragments arranged by size:
3419 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 448 973 1535 2387 2570 2603 2995 3773 3963
Size: 525 562 852 183 33 392 778 190
Cuts at: 3963 4755 448
Size: 792 1674
Fragments arranged by size:
1674 852 792 778 562 525 392 190
183 33
BsmI GAATG_Cn'
Cuts at: 1698 1698
Size: 5981
BsmBI CGTCTCn'nnnn_
Cuts at: 2294 2294
Size: 5981
Bsp1286I G_dGCh'C
Cuts at: 448 760 973 1535 1872 2018 2387 2434 2570
Size: 312 213 562 337 146 369 47 136
Cuts at: 2570 2603 2645 2690 2701 2962 2995 3606 3699
Size: 33 42 45 11 261 33 611 93
Cuts at: 3699 3773 3963 4025 4755 448
Size: 74 190 62 730 1674
Fragments arranged by size:
1674 730 611 562 369 337 312 261
213 190 146 136 93 74 62 47
45 42 33 33 11
BspEI T'CCGG_A
Cuts at: 1666 2905 5201 1666
Size: 1239 2296 2446
Fragments arranged by size:
2446 2296 1239
BspHI T'CATG_A
Cuts at: 2753 5157 2753
Size: 2404 3577
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2456 2565 2612 3031 3245 3547 3928 2456
Size: 109 47 419 214 302 381 4509
Fragments arranged by size:
4509 419 381 302 214 109 47
BsrBI CCG'CTC
Cuts at: 50 1326 1616 2331 4273 4327 5811 5976 50
Size: 1276 290 715 1942 54 1484 165 55
Fragments arranged by size:
1942 1484 1276 715 290 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2349 3893 2349
Size: 1544 4437
BsrFI r'CCGG_y
Cuts at: 1492 1649 1805 2253 2416 2935 3979 4160 4369
Size: 157 156 448 163 519 1044 181 209
Cuts at: 4369 1492
Size: 3104
Fragments arranged by size:
3104 1044 519 448 209 181 163 157
156
BssHII G'CGCG_C
Cuts at: 1853 1853
Size: 5981
BssSI C'ACGA_G
Cuts at: 1443 4252 4610 1443
Size: 2809 358 2814
Fragments arranged by size:
2814 2809 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2738 3358 2738
Size: 620 5361
BstBI TT'CG_AA
Cuts at: 4342 4342
Size: 5981
BstXI CCAn_nnnn'nTGG
Cuts at: 162 1488 162
Size: 1326 4655
BstYI r'GATC_y
Cuts at: 1 2309 3006 3473 3496 3831 4077 5089 5175
Size: 2308 697 467 23 335 246 1012 86
Cuts at: 5175 5187 5533 1
Size: 12 346 449
Fragments arranged by size:
2308 1012 697 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1660 1660
Size: 5981
BtgI C'CryG_G
Cuts at: 161 718 2019 2425 2681 161
Size: 557 1301 406 256 3461
Fragments arranged by size:
3461 1301 557 406 256
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2216 3931 4112 4275 2216
Size: 1715 181 163 3922
Fragments arranged by size:
3922 1715 181 163
BtsI GCAGTG_nn'
Cuts at: 2385 3224 2385
Size: 839 5142
DraI TTT'AAA
Cuts at: 2980 2980
Size: 5981
DraIII CAC_nnn'GTG
Cuts at: 2968 2968
Size: 5981
DrdI GACnn_nn'nnGTC
Cuts at: 3687 4545 3687
Size: 858 5123
EaeI y'GGCC_r
Cuts at: 438 552 1490 2775 3566 3740 4131 4158 5503
Size: 114 938 1285 791 174 391 27 1345
Cuts at: 5503 438
Size: 916
Fragments arranged by size:
1345 1285 938 916 791 391 174 114
27
EagI C'GGCC_G
Cuts at: 3566 3566
Size: 5981
EarI CTCTTCn'nnn_
Cuts at: 984 1232 1382 1403 1919 2710 2825 4004 4214
Size: 248 150 21 516 791 115 1179 210
Cuts at: 4214 984
Size: 2751
Fragments arranged by size:
2751 1179 791 516 248 210 150 115
21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1315 2074 4189 4499 4645 5791 1315
Size: 759 2115 310 146 1146 1505
Fragments arranged by size:
2115 1505 1146 759 310 146
EcoICRI GAG'CTC
Cuts at: 971 2993 971
Size: 2022 3959
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 142 519 542 644 1372 1588 2116 2230
Size: 9 377 23 102 728 216 528 114
Cuts at: 2230 2263 2532 2734 2810 3806 4238 4985 5253
Size: 33 269 202 76 996 432 747 268
Cuts at: 5253 133
Size: 861
Fragments arranged by size:
996 861 747 728 528 432 377 269
268 216 202 114 102 76 33 23
9
EcoNI CCTnn'n_nnAGG
Cuts at: 2032 2125 2032
Size: 93 5888
EcoO109I rG'GnC_Cy
Cuts at: 394 1476 2686 3120 394
Size: 1082 1210 434 3255
Fragments arranged by size:
3255 1210 1082 434
EcoRI G'AATT_C
Cuts at: 2985 2985
Size: 5981
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 414 4000 414
Size: 3586 2395
FspI TGC'GCA
Cuts at: 2196 3762 2196
Size: 1566 4415
HaeII r_GCGC'y
Cuts at: 136 143 231 2139 3415 3663 4685 5664 136
Size: 7 88 1908 1276 248 1022 979 453
Fragments arranged by size:
1908 1276 1022 979 453 248 88 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 250 282 1930 1962 3823 3853 3855 3885 250
Size: 32 1648 32 1861 30 2 30 2346
Fragments arranged by size:
2346 1861 1648 32 32 30 30 2
HincII GTy'rAC
Cuts at: 384 3020 384
Size: 2636 3345
KasI G'GCGC_C
Cuts at: 132 139 3411 3659 132
Size: 7 3272 248 2454
Fragments arranged by size:
3272 2454 248 7
KpnI G_GTAC'C
Cuts at: 1826 2028 3001 1826
Size: 202 973 4806
Fragments arranged by size:
4806 973 202
MluI A'CGCG_T
Cuts at: 5414 5414
Size: 5981
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 760 1537 4652 4836 760
Size: 777 3115 184 1905
Fragments arranged by size:
3115 1905 777 184
MscI TGG'CCA
Cuts at: 440 554 3742 440
Size: 114 3188 2679
Fragments arranged by size:
3188 2679 114
MslI CAynn'nnrTG
Cuts at: 160 2342 3970 4097 160
Size: 2182 1628 127 2044
Fragments arranged by size:
2182 2044 1628 127
MspA1I CmG'CkG
Cuts at: 1311 1590 2322 2427 2728 3092 3406 3766 4779
Size: 279 732 105 301 364 314 360 1013
Cuts at: 4779 5024 1311
Size: 245 2268
Fragments arranged by size:
2268 1013 732 364 360 314 301 279
245 105
NaeI GCC'GGC
Cuts at: 1494 1651 2255 4162 1494
Size: 157 604 1907 3313
Fragments arranged by size:
3313 1907 604 157
NarI GG'CG_CC
Cuts at: 133 140 3412 3660 133
Size: 7 3272 248 2454
Fragments arranged by size:
3272 2454 248 7
NcoI C'CATG_G
Cuts at: 161 2019 161
Size: 1858 4123
NgoMIV G'CCGG_C
Cuts at: 1492 1649 2253 4160 1492
Size: 157 604 1907 3313
Fragments arranged by size:
3313 1907 604 157
NspI r_CATG'y
Cuts at: 267 2739 3034 3534 4441 267
Size: 2472 295 500 907 1807
Fragments arranged by size:
2472 1807 907 500 295
PasI CC'CwG_GG
Cuts at: 1865 1865
Size: 5981
PciI A'CATG_T
Cuts at: 263 4437 263
Size: 4174 1807
PflMI CCAn_nnn'nTGG
Cuts at: 2108 2108
Size: 5981
PfoI T'CCnGG_A
Cuts at: 621 1886 2240 2369 2378 2864 621
Size: 1265 354 129 9 486 3738
Fragments arranged by size:
3738 1265 486 354 129 9
PmeI GTTT'AAAC
Cuts at: 2980 2980
Size: 5981
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5120 5152 5120
Size: 32 5949
PpuMI rG'GwC_Cy
Cuts at: 394 394
Size: 5981
PspOMI G'GGCC_C
Cuts at: 1868 2686 1868
Size: 818 5163
PstI C_TGCA'G
Cuts at: 1585 1790 2222 3028 3713 1585
Size: 205 432 806 685 3853
Fragments arranged by size:
3853 806 685 432 205
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5981
PvuII CAG'CTG
Cuts at: 1590 2322 3406 3766 1590
Size: 732 1084 360 3805
Fragments arranged by size:
3805 1084 732 360
RsrII CG'GwC_CG
Cuts at: 1559 4176 1559
Size: 2617 3364
SacI G_AGCT'C
Cuts at: 973 2995 973
Size: 2022 3959
SacII CC_GC'GG
Cuts at: 2428 2428
Size: 5981
SalI G'TCGA_C
Cuts at: 3018 3018
Size: 5981
SanDI GG'GwC_CC
Cuts at: 394 394
Size: 5981
SapI GCTCTTCn'nnn_
Cuts at: 4004 4214 4004
Size: 210 5771
SbfI CC_TGCA'GG
Cuts at: 3028 3028
Size: 5981
ScaI AGT'ACT
Cuts at: 5498 5498
Size: 5981
SfcI C'TryA_G
Cuts at: 33 1094 1581 1786 2218 2581 3024 3709 4702
Size: 1061 487 205 432 363 443 685 993
Cuts at: 4702 4893 33
Size: 191 1121
Fragments arranged by size:
1121 1061 993 685 487 443 432 363
205 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1204 1204
Size: 5981
SfoI GGC'GCC
Cuts at: 134 141 3413 3661 134
Size: 7 3272 248 2454
Fragments arranged by size:
3272 2454 248 7
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5981
SmaI CCC'GGG
Cuts at: 3003 3003
Size: 5981
SmlI C'TyrA_G
Cuts at: 1150 1249 1315 1363 2500 3136 4051 4543 4805
Size: 99 66 48 1137 636 915 492 262
Cuts at: 4805 1150
Size: 2326
Fragments arranged by size:
2326 1137 915 636 492 262 99 66
48
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5981
SphI G_CATG'C
Cuts at: 3034 3534 3034
Size: 500 5481
StuI AGG'CCT
Cuts at: 316 2281 316
Size: 1965 4016
StyI C'CwwG_G
Cuts at: 161 329 888 1208 2019 2102 2276 3115 3469
Size: 168 559 320 811 83 174 839 354
Cuts at: 3469 161
Size: 2673
Fragments arranged by size:
2673 839 811 559 354 320 174 168
83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 387 2761 2956 3564 3697 4156 4365 387
Size: 2374 195 608 133 459 209 2003
Fragments arranged by size:
2374 2003 608 459 209 195 133
TatI w'GTAC_w
Cuts at: 948 5496 948
Size: 4548 1433
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 109 141 5498 5530 109
Size: 32 5357 32 560
Fragments arranged by size:
5357 560 32 32
Tth111I GACn'n_nGTC
Cuts at: 3778 3778
Size: 5981
XbaI T'CTAG_A
Cuts at: 3012 3012
Size: 5981
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2890 3476 2890
Size: 586 5395
XmaI C'CCGG_G
Cuts at: 3001 3001
Size: 5981
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AhdI AleI AlfI AloI AscI AvrII BbvCI
BfrBI BmgBI BmtI BsiWI BsrGI BstEII BstZ17I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PmlI PshAI PsiI
PspXI PsrI SexAI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI