Restriction map for
pF1KB8528
(Circular) (Six-base) MAPSORT of: pF1KB8528.seq Check: 3502 from: 1 to: 5768
pF1KB8528 5768 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1497 1497
Size: 5768
AatII G_ACGT'C
Cuts at: 806 806
Size: 5768
AccI GT'mk_AC
Cuts at: 2806 2806
Size: 5768
Acc65I G'GTAC_C
Cuts at: 2050 2784 2050
Size: 734 5034
AclI AA'CG_TT
Cuts at: 5608 5608
Size: 5768
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 1751 1880 2126 2724 3593 4025 4772 5040
Size: 1417 129 246 598 869 432 747 268
Cuts at: 5040 334
Size: 1062
Fragments arranged by size:
1417 1062 869 747 598 432 268 246
129
AflIII A'CryG_T
Cuts at: 863 1151 1262 3120 4224 5201 863
Size: 288 111 1858 1104 977 1430
Fragments arranged by size:
1858 1430 1104 977 288 111
AgeI A'CCGG_T
Cuts at: 4156 4156
Size: 5768
AhdI GACnn_n'nnGTC
Cuts at: 2416 2416
Size: 5768
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 385 2366 385
Size: 1981 3787
AlwNI CAG_nnn'CTG
Cuts at: 1644 3073 4640 1644
Size: 1429 1567 2772
Fragments arranged by size:
2772 1567 1429
ApaLI G'TGCA_C
Cuts at: 4538 4538
Size: 5768
ApoI r'AATT_y
Cuts at: 1033 1097 1546 2507 2772 5121 5377 1033
Size: 64 449 961 265 2349 256 1424
Fragments arranged by size:
2349 1424 961 449 265 256 64
AseI AT'TA_AT
Cuts at: 20 20
Size: 5768
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5768
AvaI C'yCGr_G
Cuts at: 125 1135 1396 1418 1835 2354 2788 125
Size: 1010 261 22 417 519 434 3105
Fragments arranged by size:
3105 1010 519 434 417 261 22
BamHI G'GATC_C
Cuts at: 2793 2793
Size: 5768
BanI G'GyrC_C
Cuts at: 111 2050 2784 3198 3446 3481 111
Size: 1939 734 414 248 35 2398
Fragments arranged by size:
2398 1939 734 414 248 35
BanII G_rGCy'C
Cuts at: 134 2782 3812 134
Size: 2648 1030 2090
Fragments arranged by size:
2648 2090 1030
BbeI G_GCGC'C
Cuts at: 115 3202 3450 115
Size: 3087 248 2433
Fragments arranged by size:
3087 2433 248
BbsI GAAGACnn'nnnn_
Cuts at: 434 1121 2114 434
Size: 687 993 4088
Fragments arranged by size:
4088 993 687
BbvCI CC'TCA_GC
Cuts at: 656 656
Size: 5768
BciVI GTATCCnnnnn_n'
Cuts at: 3666 4427 5768 3666
Size: 761 1341 3666
Fragments arranged by size:
3666 1341 761
BclI T'GATC_A
Cuts at: 3288 3288
Size: 5768
BfrBI ATG'CAT
Cuts at: 2663 2663
Size: 5768
BglII A'GATC_T
Cuts at: 3283 5320 3283
Size: 2037 3731
BlpI GC'TnA_GC
Cuts at: 1408 2339 2880 1408
Size: 931 541 4296
Fragments arranged by size:
4296 931 541
Bme1580I G_kGCm'C
Cuts at: 3393 3486 4542 3393
Size: 93 1056 4619
Fragments arranged by size:
4619 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 113 933 1771 1892 2212 2980 3061 3396 5540
Size: 820 838 121 320 768 81 335 2144
Cuts at: 5540 113
Size: 341
Fragments arranged by size:
2144 838 820 768 341 335 320 121
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1074 4135 1074
Size: 3061 2707
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 1486 1674 490
Size: 996 188 4584
Fragments arranged by size:
4584 996 188
Bpu10I CC'TnA_GC
Cuts at: 656 656
Size: 5768
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 646 731 1525 1837 2944 3823 4315 4613 646
Size: 85 794 312 1107 879 492 298 1801
Fragments arranged by size:
1801 1107 879 794 492 312 298 85
BsaI GGTCTCn'nnnn_
Cuts at: 5472 5472
Size: 5768
BsaAI yAC'GTr
Cuts at: 3121 3121
Size: 5768
BsaBI GATnn'nnATC
Cuts at: 6 1274 1964 3282 5319 6
Size: 1268 690 1318 2037 455
Fragments arranged by size:
2037 1318 1268 690 455
BsaHI Gr'CG_yC
Cuts at: 112 200 803 3199 3447 4149 5645 112
Size: 88 603 2396 248 702 1496 235
Fragments arranged by size:
2396 1496 702 603 248 235 88
BsaWI w'CCGG_w
Cuts at: 1956 2957 3478 4156 4430 4577 4988 1956
Size: 1001 521 678 274 147 411 2736
Fragments arranged by size:
2736 1001 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 164 194 1927 1957 164
Size: 30 1733 30 3975
Fragments arranged by size:
3975 1733 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1414 1414
Size: 5768
BseYI C'CCAG_C
Cuts at: 845 906 1182 2046 3193 3951 4528 5024 845
Size: 61 276 864 1147 758 577 496 1589
Fragments arranged by size:
1589 1147 864 758 577 496 276 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 797 1519 797
Size: 722 5046
BsiEI CG_ry'CG
Cuts at: 104 3310 3356 4564 104
Size: 3206 46 1208 1308
Fragments arranged by size:
3206 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1055 2217 2782 3560 3750 4542 1055
Size: 1162 565 778 190 792 2281
Fragments arranged by size:
2281 1162 792 778 565 190
BsmBI CGTCTCn'nnnn_
Cuts at: 2226 2226
Size: 5768
Bsp1286I G_dGCh'C
Cuts at: 134 1055 2217 2782 3393 3486 3560 3750 3812
Size: 921 1162 565 611 93 74 190 62
Cuts at: 3812 4542 134
Size: 730 1360
Fragments arranged by size:
1360 1162 921 730 611 565 190 93
74 62
BspEI T'CCGG_A
Cuts at: 1956 4988 1956
Size: 3032 2736
BspHI T'CATG_A
Cuts at: 4944 4944
Size: 5768
BspMI ACCTGCnnnn'nnnn_
Cuts at: 537 840 1093 1445 1497 2406 2818 3032 3334
Size: 303 253 352 52 909 412 214 302
Cuts at: 3334 3715 537
Size: 381 2590
Fragments arranged by size:
2590 909 412 381 352 303 302 253
214 52
BsrBI CCG'CTC
Cuts at: 50 1886 4060 4114 5598 5763 50
Size: 1836 2174 54 1484 165 55
Fragments arranged by size:
2174 1836 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2663 3680 2663
Size: 1017 4751
BsrFI r'CCGG_y
Cuts at: 538 3766 3947 4156 538
Size: 3228 181 209 2150
Fragments arranged by size:
3228 2150 209 181
BsrGI T'GTAC_A
Cuts at: 1154 1154
Size: 5768
BssSI C'ACGA_G
Cuts at: 997 4039 4397 997
Size: 3042 358 2368
Fragments arranged by size:
3042 2368 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1497 2421 2660 3145 1497
Size: 924 239 485 4120
Fragments arranged by size:
4120 924 485 239
BstBI TT'CG_AA
Cuts at: 1143 4129 1143
Size: 2986 2782
BstYI r'GATC_y
Cuts at: 1 1987 2793 3260 3283 3618 3864 4876 4962
Size: 1986 806 467 23 335 246 1012 86
Cuts at: 4962 4974 5320 1
Size: 12 346 449
Fragments arranged by size:
1986 1012 806 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 501 791 1742 1751 501
Size: 290 951 9 4518
Fragments arranged by size:
4518 951 290 9
BtgI C'CryG_G
Cuts at: 107 233 378 107
Size: 126 145 5497
Fragments arranged by size:
5497 145 126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 293 3718 3899 4062 293
Size: 3425 181 163 1999
Fragments arranged by size:
3425 1999 181 163
BtsI GCAGTG_nn'
Cuts at: 923 3011 923
Size: 2088 3680
DraI TTT'AAA
Cuts at: 2767 2767
Size: 5768
DrdI GACnn_nn'nnGTC
Cuts at: 802 3474 4332 802
Size: 2672 858 2238
Fragments arranged by size:
2672 2238 858
EaeI y'GGCC_r
Cuts at: 2752 3353 3527 3918 3945 5290 2752
Size: 601 174 391 27 1345 3230
Fragments arranged by size:
3230 1345 601 391 174 27
EagI C'GGCC_G
Cuts at: 3353 3353
Size: 5768
EarI CTCTTCn'nnn_
Cuts at: 953 1876 2023 2437 3791 4001 953
Size: 923 147 414 1354 210 2720
Fragments arranged by size:
2720 1354 923 414 210 147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3976 4286 4432 5578 3976
Size: 310 146 1146 4166
Fragments arranged by size:
4166 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2780 2780
Size: 5768
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 490 1486 1674 1751 1880 2126 2724 3593
Size: 156 996 188 77 129 246 598 869
Cuts at: 3593 4025 4772 5040 334
Size: 432 747 268 1062
Fragments arranged by size:
1062 996 869 747 598 432 268 246
188 156 129 77
EcoO109I rG'GnC_Cy
Cuts at: 138 261 549 2526 2907 138
Size: 123 288 1977 381 2999
Fragments arranged by size:
2999 1977 381 288 123
EcoRI G'AATT_C
Cuts at: 2772 2772
Size: 5768
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3787 3787
Size: 5768
FspI TGC'GCA
Cuts at: 3549 3549
Size: 5768
HaeII r_GCGC'y
Cuts at: 115 3202 3450 4472 5451 115
Size: 3087 248 1022 979 432
Fragments arranged by size:
3087 1022 979 432 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 484 516 644 676 2402 2434 3610 3640 3642
Size: 32 128 32 1726 32 1176 30 2
Cuts at: 3642 3672 484
Size: 30 2580
Fragments arranged by size:
2580 1726 1176 128 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1044 1628 2807 1044
Size: 584 1179 4005
Fragments arranged by size:
4005 1179 584
KasI G'GCGC_C
Cuts at: 111 3198 3446 111
Size: 3087 248 2433
Fragments arranged by size:
3087 2433 248
KpnI G_GTAC'C
Cuts at: 2054 2788 2054
Size: 734 5034
MluI A'CGCG_T
Cuts at: 5201 5201
Size: 5768
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 191 675 713 2257 2433 2740 4439 4623 191
Size: 484 38 1544 176 307 1699 184 1336
Fragments arranged by size:
1699 1544 1336 484 307 184 176 38
MscI TGG'CCA
Cuts at: 2754 3529 2754
Size: 775 4993
MslI CAynn'nnrTG
Cuts at: 3757 3884 3757
Size: 127 5641
MspA1I CmG'CkG
Cuts at: 572 1368 1503 1641 1893 2074 2338 2350 2879
Size: 796 135 138 252 181 264 12 529
Cuts at: 2879 3193 3553 4566 4811 572
Size: 314 360 1013 245 1529
Fragments arranged by size:
1529 1013 796 529 360 314 264 252
245 181 138 135 12
NaeI GCC'GGC
Cuts at: 540 3949 540
Size: 3409 2359
NarI GG'CG_CC
Cuts at: 112 3199 3447 112
Size: 3087 248 2433
Fragments arranged by size:
3087 2433 248
NcoI C'CATG_G
Cuts at: 107 378 107
Size: 271 5497
NgoMIV G'CCGG_C
Cuts at: 538 3947 538
Size: 3409 2359
NsiI A_TGCA'T
Cuts at: 2665 2665
Size: 5768
NspI r_CATG'y
Cuts at: 867 1155 1266 2060 2709 2821 3321 4228 867
Size: 288 111 794 649 112 500 907 2407
Fragments arranged by size:
2407 907 794 649 500 288 112 111
PasI CC'CwG_GG
Cuts at: 134 134
Size: 5768
PciI A'CATG_T
Cuts at: 863 1151 1262 4224 863
Size: 288 111 2962 2407
Fragments arranged by size:
2962 2407 288 111
PflMI CCAn_nnn'nTGG
Cuts at: 274 1984 274
Size: 1710 4058
PfoI T'CCnGG_A
Cuts at: 194 194
Size: 5768
PmeI GTTT'AAAC
Cuts at: 2767 2767
Size: 5768
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4907 4939 4907
Size: 32 5736
PpuMI rG'GwC_Cy
Cuts at: 138 549 2526 138
Size: 411 1977 3380
Fragments arranged by size:
3380 1977 411
PspXI vC'TCGA_Gb
Cuts at: 1135 1135
Size: 5768
PstI C_TGCA'G
Cuts at: 1508 2388 2815 3500 1508
Size: 880 427 685 3776
Fragments arranged by size:
3776 880 685 427
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5768
PvuII CAG'CTG
Cuts at: 572 1368 1503 1641 2074 2338 2350 3193 3553
Size: 796 135 138 433 264 12 843 360
Cuts at: 3553 572
Size: 2787
Fragments arranged by size:
2787 843 796 433 360 264 138 135
12
RsrII CG'GwC_CG
Cuts at: 1888 3963 1888
Size: 2075 3693
SacI G_AGCT'C
Cuts at: 2782 2782
Size: 5768
SalI G'TCGA_C
Cuts at: 2805 2805
Size: 5768
SapI GCTCTTCn'nnn_
Cuts at: 1876 3791 4001 1876
Size: 1915 210 3643
Fragments arranged by size:
3643 1915 210
SbfI CC_TGCA'GG
Cuts at: 2815 2815
Size: 5768
ScaI AGT'ACT
Cuts at: 5285 5285
Size: 5768
SfcI C'TryA_G
Cuts at: 33 953 1231 1504 1590 2384 2565 2811 3496
Size: 920 278 273 86 794 181 246 685
Cuts at: 3496 4489 4680 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 920 794 685 278 273 246
191 181 86
SfoI GGC'GCC
Cuts at: 113 3200 3448 113
Size: 3087 248 2433
Fragments arranged by size:
3087 2433 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5768
SmaI CCC'GGG
Cuts at: 127 1420 2356 2790 127
Size: 1293 936 434 3105
Fragments arranged by size:
3105 1293 936 434
SmlI C'TyrA_G
Cuts at: 661 746 1135 1540 1852 2923 3838 4330 4592
Size: 85 389 405 312 1071 915 492 262
Cuts at: 4592 661
Size: 1837
Fragments arranged by size:
1837 1071 915 492 405 389 312 262
85
SpeI A'CTAG_T
Cuts at: 67 444 67
Size: 377 5391
SphI G_CATG'C
Cuts at: 2821 3321 2821
Size: 500 5268
StyI C'CwwG_G
Cuts at: 107 248 378 2467 2902 3256 107
Size: 141 130 2089 435 354 2619
Fragments arranged by size:
2619 2089 435 354 141 130
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 132 371 1444 3351 3484 3943 4152 132
Size: 239 1073 1907 133 459 209 1748
Fragments arranged by size:
1907 1748 1073 459 239 209 133
TatI w'GTAC_w
Cuts at: 1154 1698 2298 5283 1154
Size: 544 600 2985 1639
Fragments arranged by size:
2985 1639 600 544
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 164 196 1927 1959 5285 5317 164
Size: 32 1731 32 3326 32 615
Fragments arranged by size:
3326 1731 615 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2646 3565 2646
Size: 919 4849
XbaI T'CTAG_A
Cuts at: 2799 2799
Size: 5768
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3263 3263
Size: 5768
XhoI C'TCGA_G
Cuts at: 1135 1135
Size: 5768
XmaI C'CCGG_G
Cuts at: 125 1418 2354 2788 125
Size: 1293 936 434 3105
Fragments arranged by size:
3105 1293 936 434
ZraI GAC'GTC
Cuts at: 804 804
Size: 5768
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AleI AloI ApaI AscI AvrII BaeI
BcgI BglI BmgBI BmtI BsiWI BsmI BssHII BstEII
BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
PacI PmlI PshAI PsiI PspOMI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XmnI