Restriction map for
pF1KB8532
(Circular) (Six-base) MAPSORT of: pF1KB8532.seq Check: 2689 from: 1 to: 5807
pF1KB8532 5807 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AccI GT'mk_AC
Cuts at: 557 2845 557
Size: 2288 3519
Acc65I G'GTAC_C
Cuts at: 2823 2823
Size: 5807
AclI AA'CG_TT
Cuts at: 5647 5647
Size: 5807
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 796 1363 1541 3632 4064 4811 5079 796
Size: 567 178 2091 432 747 268 1524
Fragments arranged by size:
2091 1524 747 567 432 268 178
AfeI AGC'GCT
Cuts at: 1780 1780
Size: 5807
AflIII A'CryG_T
Cuts at: 3159 4263 5240 3159
Size: 1104 977 3726
Fragments arranged by size:
3726 1104 977
AgeI A'CCGG_T
Cuts at: 2292 4195 2292
Size: 1903 3904
AleI CACnn'nnGTG
Cuts at: 823 823
Size: 5807
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 841 976 841
Size: 135 5672
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 196 228 2680 2712 196
Size: 32 2452 32 3291
Fragments arranged by size:
3291 2452 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3112 4679 3112
Size: 1567 4240
ApaI G_GGCC'C
Cuts at: 1385 1451 1385
Size: 66 5741
ApaLI G'TGCA_C
Cuts at: 816 4577 816
Size: 3761 2046
ApoI r'AATT_y
Cuts at: 122 1957 2536 2811 5160 5416 122
Size: 1835 579 275 2349 256 513
Fragments arranged by size:
2349 1835 579 513 275 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5807
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5807
AvaI C'yCGr_G
Cuts at: 859 1533 2358 2497 2700 2827 859
Size: 674 825 139 203 127 3839
Fragments arranged by size:
3839 825 674 203 139 127
AvrII C'CTAG_G
Cuts at: 1107 1107
Size: 5807
BamHI G'GATC_C
Cuts at: 2512 2832 2512
Size: 320 5487
BanI G'GyrC_C
Cuts at: 2095 2295 2823 3237 3485 3520 2095
Size: 200 528 414 248 35 4382
Fragments arranged by size:
4382 528 414 248 200 35
BanII G_rGCy'C
Cuts at: 866 1304 1385 1451 1736 1748 1932 2407 2821
Size: 438 81 66 285 12 184 475 414
Cuts at: 2821 3851 866
Size: 1030 2822
Fragments arranged by size:
2822 1030 475 438 414 285 184 81
66 12
BbeI G_GCGC'C
Cuts at: 2099 3241 3489 2099
Size: 1142 248 4417
Fragments arranged by size:
4417 1142 248
BbsI GAAGACnn'nnnn_
Cuts at: 355 536 355
Size: 181 5626
BbvCI CC'TCA_GC
Cuts at: 1556 1556
Size: 5807
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 547 581 1416 1450 90
Size: 34 423 34 835 34 4447
Fragments arranged by size:
4447 835 423 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3705 4466 5807 3705
Size: 761 1341 3705
Fragments arranged by size:
3705 1341 761
BclI T'GATC_A
Cuts at: 3327 3327
Size: 5807
BglI GCCn_nnn'nGGC
Cuts at: 2567 2567
Size: 5807
BglII A'GATC_T
Cuts at: 3322 5359 3322
Size: 2037 3770
BlpI GC'TnA_GC
Cuts at: 1967 2919 1967
Size: 952 4855
Bme1580I G_kGCm'C
Cuts at: 311 820 830 1385 1451 2021 2300 3432 3525
Size: 509 10 555 66 570 279 1132 93
Cuts at: 3525 4581 311
Size: 1056 1537
Fragments arranged by size:
1537 1132 1056 570 555 509 279 93
66 10
BmrI ACTGGGnnnn_n'
Cuts at: 1156 1388 1591 1696 1927 3019 3100 3435 5579
Size: 232 203 105 231 1092 81 335 2144
Cuts at: 5579 1156
Size: 1384
Fragments arranged by size:
2144 1384 1092 335 232 231 203 105
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1767 4174 1767
Size: 2407 3400
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 457 683 955 211
Size: 246 226 272 5063
Fragments arranged by size:
5063 272 246 226
Bpu10I CC'TnA_GC
Cuts at: 1556 2586 1556
Size: 1030 4777
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 825 1720 2983 3862 4354 4652 403
Size: 422 895 1263 879 492 298 1558
Fragments arranged by size:
1558 1263 895 879 492 422 298
BsaI GGTCTCn'nnnn_
Cuts at: 1555 1750 5511 1555
Size: 195 3761 1851
Fragments arranged by size:
3761 1851 195
BsaAI yAC'GTr
Cuts at: 1476 3160 1476
Size: 1684 4123
BsaBI GATnn'nnATC
Cuts at: 6 3321 5358 6
Size: 3315 2037 455
Fragments arranged by size:
3315 2037 455
BsaHI Gr'CG_yC
Cuts at: 730 946 2096 3238 3486 4188 5684 730
Size: 216 1150 1142 248 702 1496 853
Fragments arranged by size:
1496 1150 1142 853 702 248 216
BsaWI w'CCGG_w
Cuts at: 2292 2996 3517 4195 4469 4616 5027 2292
Size: 704 521 678 274 147 411 3072
Fragments arranged by size:
3072 704 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1234 1264 2467 2497 2680 2710 1234
Size: 30 1203 30 183 30 4331
Fragments arranged by size:
4331 1203 183 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 199 1246 1258 1303 1573 2238 2640 199
Size: 1047 12 45 270 665 402 3366
Fragments arranged by size:
3366 1047 665 402 270 45 12
BseYI C'CCAG_C
Cuts at: 337 1255 2128 2311 2731 3232 3990 4567 5063
Size: 918 873 183 420 501 758 577 496
Cuts at: 5063 337
Size: 1081
Fragments arranged by size:
1081 918 873 758 577 501 496 420
183
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1456 1456
Size: 5807
BsiEI CG_ry'CG
Cuts at: 104 3349 3395 4603 104
Size: 3245 46 1208 1308
Fragments arranged by size:
3245 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 359 820 866 1748 2821 3599 3789 4581 359
Size: 461 46 882 1073 778 190 792 1585
Fragments arranged by size:
1585 1073 882 792 778 461 190 46
BsmI GAATG_Cn'
Cuts at: 159 1323 2124 2202 2217 159
Size: 1164 801 78 15 3749
Fragments arranged by size:
3749 1164 801 78 15
Bsp1286I G_dGCh'C
Cuts at: 311 359 820 830 866 1304 1385 1451 1736
Size: 48 461 10 36 438 81 66 285
Cuts at: 1736 1748 1932 2021 2300 2407 2821 3432 3525
Size: 12 184 89 279 107 414 611 93
Cuts at: 3525 3599 3789 3851 4581 311
Size: 74 190 62 730 1537
Fragments arranged by size:
1537 730 611 461 438 414 285 279
190 184 107 93 89 81 74 66
62 48 36 12 10
BspEI T'CCGG_A
Cuts at: 5027 5027
Size: 5807
BspHI T'CATG_A
Cuts at: 4983 4983
Size: 5807
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2857 3071 3373 3754 2857
Size: 214 302 381 4910
Fragments arranged by size:
4910 381 302 214
BsrBI CCG'CTC
Cuts at: 50 225 4099 4153 5637 5802 50
Size: 175 3874 54 1484 165 55
Fragments arranged by size:
3874 1484 175 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3719 3719
Size: 5807
BsrFI r'CCGG_y
Cuts at: 2122 2292 3805 3986 4195 2122
Size: 170 1513 181 209 3734
Fragments arranged by size:
3734 1513 209 181 170
BssSI C'ACGA_G
Cuts at: 4078 4436 4078
Size: 358 5449
BstAPI GCAn_nnn'nTGC
Cuts at: 1071 3184 1071
Size: 2113 3694
BstBI TT'CG_AA
Cuts at: 4168 4168
Size: 5807
BstXI CCAn_nnnn'nTGG
Cuts at: 1413 1825 1413
Size: 412 5395
BstYI r'GATC_y
Cuts at: 1 432 1181 2512 2832 3299 3322 3657 3903
Size: 431 749 1331 320 467 23 335 246
Cuts at: 3903 4915 5001 5013 5359 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1331 1012 749 467 449 431 346 335
320 246 86 23 12
BtgI C'CryG_G
Cuts at: 2103 2454 2103
Size: 351 5456
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3757 3938 4101 3757
Size: 181 163 5463
Fragments arranged by size:
5463 181 163
BtsI GCAGTG_nn'
Cuts at: 1069 1586 1646 3050 1069
Size: 517 60 1404 3826
Fragments arranged by size:
3826 1404 517 60
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2529 2564 2529
Size: 35 5772
DraI TTT'AAA
Cuts at: 2806 2806
Size: 5807
DraIII CAC_nnn'GTG
Cuts at: 1057 1057
Size: 5807
DrdI GACnn_nn'nnGTC
Cuts at: 3513 4371 3513
Size: 858 4949
EaeI y'GGCC_r
Cuts at: 491 503 890 1089 1463 1478 2709 3392 3566
Size: 12 387 199 374 15 1231 683 174
Cuts at: 3566 3957 3984 5329 491
Size: 391 27 1345 969
Fragments arranged by size:
1345 1231 969 683 391 387 374 199
174 27 15 12
EagI C'GGCC_G
Cuts at: 3392 3392
Size: 5807
EarI CTCTTCn'nnn_
Cuts at: 1717 1918 2043 3830 4040 1717
Size: 201 125 1787 210 3484
Fragments arranged by size:
3484 1787 210 201 125
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2525 2645 4015 4325 4471 5617 2525
Size: 120 1370 310 146 1146 2715
Fragments arranged by size:
2715 1370 1146 310 146 120
EcoICRI GAG'CTC
Cuts at: 864 1746 2819 864
Size: 882 1073 3852
Fragments arranged by size:
3852 1073 882
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 457 683 796 955 1363 1541 3632 4064
Size: 246 226 113 159 408 178 2091 432
Cuts at: 4064 4811 5079 211
Size: 747 268 939
Fragments arranged by size:
2091 939 747 432 408 268 246 226
178 159 113
EcoNI CCTnn'n_nnAGG
Cuts at: 418 484 418
Size: 66 5741
EcoO109I rG'GnC_Cy
Cuts at: 408 810 838 918 1448 1999 2946 408
Size: 402 28 80 530 551 947 3269
Fragments arranged by size:
3269 947 551 530 402 80 28
EcoRI G'AATT_C
Cuts at: 2811 2811
Size: 5807
EcoRV GAT'ATC
Cuts at: 1047 1047
Size: 5807
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 444 3826 444
Size: 3382 2425
FspI TGC'GCA
Cuts at: 1061 3588 1061
Size: 2527 3280
HaeII r_GCGC'y
Cuts at: 232 1782 2099 3241 3489 4511 5490 232
Size: 1550 317 1142 248 1022 979 549
Fragments arranged by size:
1550 1142 1022 979 549 317 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1234 1266 3649 3679 3681 3711 1234
Size: 32 2383 30 2 30 3330
Fragments arranged by size:
3330 2383 32 30 30 2
HincII GTy'rAC
Cuts at: 558 681 2846 558
Size: 123 2165 3519
Fragments arranged by size:
3519 2165 123
HindIII A'AGCT_T
Cuts at: 1879 1879
Size: 5807
KasI G'GCGC_C
Cuts at: 2095 3237 3485 2095
Size: 1142 248 4417
Fragments arranged by size:
4417 1142 248
KpnI G_GTAC'C
Cuts at: 2827 2827
Size: 5807
MluI A'CGCG_T
Cuts at: 5240 5240
Size: 5807
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 199 269 762 1856 2540 4478 4662 199
Size: 70 493 1094 684 1938 184 1344
Fragments arranged by size:
1938 1344 1094 684 493 184 70
MscI TGG'CCA
Cuts at: 505 3568 505
Size: 3063 2744
MslI CAynn'nnrTG
Cuts at: 304 823 958 1411 3796 3923 304
Size: 519 135 453 2385 127 2188
Fragments arranged by size:
2385 2188 519 453 135 127
MspA1I CmG'CkG
Cuts at: 1341 2918 3232 3592 4605 4850 1341
Size: 1577 314 360 1013 245 2298
Fragments arranged by size:
2298 1577 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3988 3988
Size: 5807
NarI GG'CG_CC
Cuts at: 2096 3238 3486 2096
Size: 1142 248 4417
Fragments arranged by size:
4417 1142 248
NcoI C'CATG_G
Cuts at: 2103 2454 2103
Size: 351 5456
NgoMIV G'CCGG_C
Cuts at: 3986 3986
Size: 5807
NspI r_CATG'y
Cuts at: 2860 3360 4267 2860
Size: 500 907 4400
Fragments arranged by size:
4400 907 500
PasI CC'CwG_GG
Cuts at: 2608 2608
Size: 5807
PciI A'CATG_T
Cuts at: 4263 4263
Size: 5807
PflMI CCAn_nnn'nTGG
Cuts at: 150 207 150
Size: 57 5750
PfoI T'CCnGG_A
Cuts at: 479 479
Size: 5807
PmeI GTTT'AAAC
Cuts at: 2806 2806
Size: 5807
PmlI CAC'GTG
Cuts at: 1476 1476
Size: 5807
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2680 2712 4946 4978 2680
Size: 32 2234 32 3509
Fragments arranged by size:
3509 2234 32 32
PpuMI rG'GwC_Cy
Cuts at: 810 838 810
Size: 28 5779
PspOMI G'GGCC_C
Cuts at: 1381 1447 1381
Size: 66 5741
PspXI vC'TCGA_Gb
Cuts at: 1533 1533
Size: 5807
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2779 2811 2779
Size: 32 5775
PstI C_TGCA'G
Cuts at: 1358 2854 3539 1358
Size: 1496 685 3626
Fragments arranged by size:
3626 1496 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5807
PvuII CAG'CTG
Cuts at: 1341 3232 3592 1341
Size: 1891 360 3556
Fragments arranged by size:
3556 1891 360
RsrII CG'GwC_CG
Cuts at: 4002 4002
Size: 5807
SacI G_AGCT'C
Cuts at: 866 1748 2821 866
Size: 882 1073 3852
Fragments arranged by size:
3852 1073 882
SalI G'TCGA_C
Cuts at: 556 2844 556
Size: 2288 3519
SanDI GG'GwC_CC
Cuts at: 838 838
Size: 5807
SapI GCTCTTCn'nnn_
Cuts at: 3830 4040 3830
Size: 210 5597
SbfI CC_TGCA'GG
Cuts at: 2854 2854
Size: 5807
ScaI AGT'ACT
Cuts at: 5324 5324
Size: 5807
SfcI C'TryA_G
Cuts at: 33 1354 2175 2187 2409 2544 2718 2850 3535
Size: 1321 821 12 222 135 174 132 685
Cuts at: 3535 4528 4719 33
Size: 993 191 1121
Fragments arranged by size:
1321 1121 993 821 685 222 191 174
135 132 12
SfoI GGC'GCC
Cuts at: 2097 3239 3487 2097
Size: 1142 248 4417
Fragments arranged by size:
4417 1142 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5807
SgrAI Cr'CCGG_yG
Cuts at: 2292 2292
Size: 5807
SmaI CCC'GGG
Cuts at: 2499 2702 2829 2499
Size: 203 127 5477
Fragments arranged by size:
5477 203 127
SmlI C'TyrA_G
Cuts at: 418 804 859 1533 1735 2962 3877 4369 4631
Size: 386 55 674 202 1227 915 492 262
Cuts at: 4631 418
Size: 1594
Fragments arranged by size:
1594 1227 915 674 492 386 262 202
55
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5807
SphI G_CATG'C
Cuts at: 2860 3360 2860
Size: 500 5307
StuI AGG'CCT
Cuts at: 1441 1852 1441
Size: 411 5396
StyI C'CwwG_G
Cuts at: 725 1107 1818 2103 2271 2454 2658 2941 3295
Size: 382 711 285 168 183 204 283 354
Cuts at: 3295 725
Size: 3237
Fragments arranged by size:
3237 711 382 354 285 283 204 183
168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 895 915 1579 1968 3390 3523 3982 4191 895
Size: 20 664 389 1422 133 459 209 2511
Fragments arranged by size:
2511 1422 664 459 389 209 133 20
TatI w'GTAC_w
Cuts at: 1358 2795 5322 1358
Size: 1437 2527 1843
Fragments arranged by size:
2527 1843 1437
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 607 639 822 854 2202 2234 5324 5356 607
Size: 32 183 32 1348 32 3090 32 1058
Fragments arranged by size:
3090 1348 1058 183 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3604 3604
Size: 5807
XbaI T'CTAG_A
Cuts at: 1023 2838 1023
Size: 1815 3992
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 900 1473 1839 2719 3302 900
Size: 573 366 880 583 3405
Fragments arranged by size:
3405 880 583 573 366
XhoI C'TCGA_G
Cuts at: 859 1533 859
Size: 674 5133
XmaI C'CCGG_G
Cuts at: 2497 2700 2827 2497
Size: 203 127 5477
Fragments arranged by size:
5477 203 127
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AscI BaeI BfrBI BmgBI
BmtI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PshAI PsiI SacII SexAI SfiI
SnaBI SrfI SspI SwaI XmnI ZraI