Restriction map for
pF1KB8538
(Circular) (Six-base) MAPSORT of: pF1KB8538.seq Check: 4055 from: 1 to: 5807
pF1KB8538 5807 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AccI GT'mk_AC
Cuts at: 2845 2845
Size: 5807
Acc65I G'GTAC_C
Cuts at: 2823 2823
Size: 5807
AclI AA'CG_TT
Cuts at: 5647 5647
Size: 5807
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 329 569 571 1427 1585 1889 2108 3632
Size: 178 240 2 856 158 304 219 1524
Cuts at: 3632 4064 4811 5079 151
Size: 432 747 268 879
Fragments arranged by size:
1524 879 856 747 432 304 268 240
219 178 158 2
AflII C'TTAA_G
Cuts at: 1084 1084
Size: 5807
AflIII A'CryG_T
Cuts at: 1556 1853 2684 3159 4263 5240 1556
Size: 297 831 475 1104 977 2123
Fragments arranged by size:
2123 1104 977 831 475 297
AgeI A'CCGG_T
Cuts at: 4195 4195
Size: 5807
AlwNI CAG_nnn'CTG
Cuts at: 3112 4679 3112
Size: 1567 4240
ApaLI G'TGCA_C
Cuts at: 2523 4577 2523
Size: 2054 3753
ApoI r'AATT_y
Cuts at: 664 964 2290 2579 2811 5160 5416 664
Size: 300 1326 289 232 2349 256 1055
Fragments arranged by size:
2349 1326 1055 300 289 256 232
AseI AT'TA_AT
Cuts at: 20 1573 20
Size: 1553 4254
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5807
AvaI C'yCGr_G
Cuts at: 2827 2827
Size: 5807
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1694 1727 1939 1972 2131 2164 1694
Size: 33 212 33 159 33 5337
Fragments arranged by size:
5337 212 159 33 33 33
BamHI G'GATC_C
Cuts at: 2832 2832
Size: 5807
BanI G'GyrC_C
Cuts at: 580 2823 3237 3485 3520 580
Size: 2243 414 248 35 2867
Fragments arranged by size:
2867 2243 414 248 35
BanII G_rGCy'C
Cuts at: 2821 3851 2821
Size: 1030 4777
BbeI G_GCGC'C
Cuts at: 3241 3489 3241
Size: 248 5559
BbsI GAAGACnn'nnnn_
Cuts at: 1109 1990 1109
Size: 881 4926
BciVI GTATCCnnnnn_n'
Cuts at: 269 3705 4466 5807 269
Size: 3436 761 1341 269
Fragments arranged by size:
3436 1341 761 269
BclI T'GATC_A
Cuts at: 470 2591 3327 470
Size: 2121 736 2950
Fragments arranged by size:
2950 2121 736
BfrBI ATG'CAT
Cuts at: 894 894
Size: 5807
BglI GCCn_nnn'nGGC
Cuts at: 2320 2320
Size: 5807
BglII A'GATC_T
Cuts at: 1875 3322 5359 1875
Size: 1447 2037 2323
Fragments arranged by size:
2323 2037 1447
BlpI GC'TnA_GC
Cuts at: 1169 2919 1169
Size: 1750 4057
Bme1580I G_kGCm'C
Cuts at: 585 2527 3432 3525 4581 585
Size: 1942 905 93 1056 1811
Fragments arranged by size:
1942 1811 1056 905 93
BmrI ACTGGGnnnn_n'
Cuts at: 192 1542 2600 3019 3100 3435 5579 192
Size: 1350 1058 419 81 335 2144 420
Fragments arranged by size:
2144 1350 1058 420 419 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4174 4174
Size: 5807
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2021 2021
Size: 5807
Bpu10I CC'TnA_GC
Cuts at: 1442 1442
Size: 5807
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 734 911 1912 2465 2983 3862 4354 4652 734
Size: 177 1001 553 518 879 492 298 1889
Fragments arranged by size:
1889 1001 879 553 518 492 298 177
BsaI GGTCTCn'nnnn_
Cuts at: 710 5511 710
Size: 4801 1006
BsaAI yAC'GTr
Cuts at: 1559 3160 1559
Size: 1601 4206
BsaBI GATnn'nnATC
Cuts at: 6 1874 3321 5358 6
Size: 1868 1447 2037 455
Fragments arranged by size:
2037 1868 1447 455
BsaHI Gr'CG_yC
Cuts at: 3238 3486 4188 5684 3238
Size: 248 702 1496 3361
Fragments arranged by size:
3361 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2996 3517 4195 4469 4616 5027 2996
Size: 521 678 274 147 411 3776
Fragments arranged by size:
3776 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 127 157 184 214 127
Size: 30 27 30 5720
Fragments arranged by size:
5720 30 30 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 426 431 1672 426
Size: 5 1241 4561
Fragments arranged by size:
4561 1241 5
BseYI C'CCAG_C
Cuts at: 3232 3990 4567 5063 3232
Size: 758 577 496 3976
Fragments arranged by size:
3976 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1156 3349 3395 4603 104
Size: 1052 2193 46 1208 1308
Fragments arranged by size:
2193 1308 1208 1052 46
BsiHKAI G_wGCw'C
Cuts at: 846 2527 2821 3599 3789 4581 846
Size: 1681 294 778 190 792 2072
Fragments arranged by size:
2072 1681 792 778 294 190
BsmI GAATG_Cn'
Cuts at: 2569 2569
Size: 5807
Bsp1286I G_dGCh'C
Cuts at: 585 846 2527 2821 3432 3525 3599 3789 3851
Size: 261 1681 294 611 93 74 190 62
Cuts at: 3851 4581 585
Size: 730 1811
Fragments arranged by size:
1811 1681 730 611 294 261 190 93
74 62
BspEI T'CCGG_A
Cuts at: 5027 5027
Size: 5807
BspHI T'CATG_A
Cuts at: 653 1356 4983 653
Size: 703 3627 1477
Fragments arranged by size:
3627 1477 703
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1751 2857 3071 3373 3754 1751
Size: 1106 214 302 381 3804
Fragments arranged by size:
3804 1106 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4099 4153 5637 5802 50
Size: 4049 54 1484 165 55
Fragments arranged by size:
4049 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3719 3719
Size: 5807
BsrFI r'CCGG_y
Cuts at: 119 3805 3986 4195 119
Size: 3686 181 209 1731
Fragments arranged by size:
3686 1731 209 181
BsrGI T'GTAC_A
Cuts at: 1666 1666
Size: 5807
BssSI C'ACGA_G
Cuts at: 4078 4436 4078
Size: 358 5449
BstAPI GCAn_nnn'nTGC
Cuts at: 177 580 3184 177
Size: 403 2604 2800
Fragments arranged by size:
2800 2604 403
BstBI TT'CG_AA
Cuts at: 867 4168 867
Size: 3301 2506
BstEII G'GTnAC_C
Cuts at: 2206 2206
Size: 5807
BstXI CCAn_nnnn'nTGG
Cuts at: 2346 2346
Size: 5807
BstYI r'GATC_y
Cuts at: 1 1875 2696 2832 3299 3322 3657 3903 4915
Size: 1874 821 136 467 23 335 246 1012
Cuts at: 4915 5001 5013 5359 1
Size: 86 12 346 449
Fragments arranged by size:
1874 1012 821 467 449 346 335 246
136 86 23 12
BtgI C'CryG_G
Cuts at: 107 2574 107
Size: 2467 3340
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2741 3757 3938 4101 2741
Size: 1016 181 163 4447
Fragments arranged by size:
4447 1016 181 163
BtsI GCAGTG_nn'
Cuts at: 1641 1918 3050 1641
Size: 277 1132 4398
Fragments arranged by size:
4398 1132 277
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1307 1342 1307
Size: 35 5772
DraI TTT'AAA
Cuts at: 419 1799 2712 2806 419
Size: 1380 913 94 3420
Fragments arranged by size:
3420 1380 913 94
DraIII CAC_nnn'GTG
Cuts at: 1536 1536
Size: 5807
DrdI GACnn_nn'nnGTC
Cuts at: 3513 4371 3513
Size: 858 4949
EaeI y'GGCC_r
Cuts at: 1138 3392 3566 3957 3984 5329 1138
Size: 2254 174 391 27 1345 1616
Fragments arranged by size:
2254 1616 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3392 3392
Size: 5807
EarI CTCTTCn'nnn_
Cuts at: 1190 1649 1885 2768 3830 4040 1190
Size: 459 236 883 1062 210 2957
Fragments arranged by size:
2957 1062 883 459 236 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4015 4325 4471 5617 4015
Size: 310 146 1146 4205
Fragments arranged by size:
4205 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2819 2819
Size: 5807
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 329 569 571 1427 1585 1889 2021 2108
Size: 178 240 2 856 158 304 132 87
Cuts at: 2108 3632 4064 4811 5079 151
Size: 1524 432 747 268 879
Fragments arranged by size:
1524 879 856 747 432 304 268 240
178 158 132 87 2
EcoNI CCTnn'n_nnAGG
Cuts at: 1084 1654 1084
Size: 570 5237
EcoO109I rG'GnC_Cy
Cuts at: 2946 2946
Size: 5807
EcoRI G'AATT_C
Cuts at: 2290 2811 2290
Size: 521 5286
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1363 2797 3826 1363
Size: 1434 1029 3344
Fragments arranged by size:
3344 1434 1029
FspI TGC'GCA
Cuts at: 3588 3588
Size: 5807
HaeII r_GCGC'y
Cuts at: 3241 3489 4511 5490 3241
Size: 248 1022 979 3558
Fragments arranged by size:
3558 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 184 216 738 770 915 947 1152 1184 1885
Size: 32 522 32 145 32 205 32 701
Cuts at: 1885 1917 2046 2078 3649 3679 3681 3711 184
Size: 32 129 32 1571 30 2 30 2280
Fragments arranged by size:
2280 1571 701 522 205 145 129 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2846 2846
Size: 5807
HindIII A'AGCT_T
Cuts at: 2219 2219
Size: 5807
KasI G'GCGC_C
Cuts at: 3237 3485 3237
Size: 248 5559
KpnI G_GTAC'C
Cuts at: 2827 2827
Size: 5807
MfeI C'AATT_G
Cuts at: 1400 2324 2453 1400
Size: 924 129 4754
Fragments arranged by size:
4754 924 129
MluI A'CGCG_T
Cuts at: 5240 5240
Size: 5807
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 521 4478 4662 521
Size: 3957 184 1666
Fragments arranged by size:
3957 1666 184
MscI TGG'CCA
Cuts at: 3568 3568
Size: 5807
MslI CAynn'nnrTG
Cuts at: 2344 3796 3923 2344
Size: 1452 127 4228
Fragments arranged by size:
4228 1452 127
MspA1I CmG'CkG
Cuts at: 183 2115 2918 3232 3592 4605 4850 183
Size: 1932 803 314 360 1013 245 1140
Fragments arranged by size:
1932 1140 1013 803 360 314 245
NaeI GCC'GGC
Cuts at: 3988 3988
Size: 5807
NarI GG'CG_CC
Cuts at: 3238 3486 3238
Size: 248 5559
NcoI C'CATG_G
Cuts at: 107 107
Size: 5807
NgoMIV G'CCGG_C
Cuts at: 3986 3986
Size: 5807
NsiI A_TGCA'T
Cuts at: 896 896
Size: 5807
NspI r_CATG'y
Cuts at: 722 894 915 1419 1508 1857 2160 2236 2688
Size: 172 21 504 89 349 303 76 452
Cuts at: 2688 2860 3360 4267 722
Size: 172 500 907 2262
Fragments arranged by size:
2262 907 504 500 452 349 303 172
172 89 76 21
PciI A'CATG_T
Cuts at: 1853 2684 4263 1853
Size: 831 1579 3397
Fragments arranged by size:
3397 1579 831
PflMI CCAn_nnn'nTGG
Cuts at: 113 113
Size: 5807
PfoI T'CCnGG_A
Cuts at: 1598 1598
Size: 5807
PmeI GTTT'AAAC
Cuts at: 2806 2806
Size: 5807
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4946 4978 4946
Size: 32 5775
PsiI TTA'TAA
Cuts at: 557 2512 557
Size: 1955 3852
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 806 838 1118 1150 806
Size: 32 280 32 5463
Fragments arranged by size:
5463 280 32 32
PstI C_TGCA'G
Cuts at: 182 1636 1925 2854 3539 182
Size: 1454 289 929 685 2450
Fragments arranged by size:
2450 1454 929 685 289
PvuI CG_AT'CG
Cuts at: 104 1156 104
Size: 1052 4755
PvuII CAG'CTG
Cuts at: 183 2115 3232 3592 183
Size: 1932 1117 360 2398
Fragments arranged by size:
2398 1932 1117 360
RsrII CG'GwC_CG
Cuts at: 4002 4002
Size: 5807
SacI G_AGCT'C
Cuts at: 2821 2821
Size: 5807
SalI G'TCGA_C
Cuts at: 2844 2844
Size: 5807
SapI GCTCTTCn'nnn_
Cuts at: 3830 4040 3830
Size: 210 5597
SbfI CC_TGCA'GG
Cuts at: 2854 2854
Size: 5807
ScaI AGT'ACT
Cuts at: 1060 5324 1060
Size: 4264 1543
SfcI C'TryA_G
Cuts at: 33 178 479 531 916 1632 1921 2500 2850
Size: 145 301 52 385 716 289 579 350
Cuts at: 2850 3535 4528 4719 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 716 685 579 385 350 301
289 191 145 52
SfoI GGC'GCC
Cuts at: 3239 3487 3239
Size: 248 5559
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5807
SmaI CCC'GGG
Cuts at: 2829 2829
Size: 5807
SmlI C'TyrA_G
Cuts at: 749 926 1084 1891 2480 2962 3877 4369 4631
Size: 177 158 807 589 482 915 492 262
Cuts at: 4631 749
Size: 1925
Fragments arranged by size:
1925 915 807 589 492 482 262 177
158
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5807
SphI G_CATG'C
Cuts at: 1419 2160 2860 3360 1419
Size: 741 700 500 3866
Fragments arranged by size:
3866 741 700 500
SspI AAT'ATT
Cuts at: 426 2267 426
Size: 1841 3966
StyI C'CwwG_G
Cuts at: 107 488 2941 3295 107
Size: 381 2453 354 2619
Fragments arranged by size:
2619 2453 381 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1524 3390 3523 3982 4191 1524
Size: 1866 133 459 209 3140
Fragments arranged by size:
3140 1866 459 209 133
TatI w'GTAC_w
Cuts at: 908 1005 1058 1617 1666 1977 5322 908
Size: 97 53 559 49 311 3345 1393
Fragments arranged by size:
3345 1393 559 311 97 53 49
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 127 159 5324 5356 127
Size: 32 5165 32 578
Fragments arranged by size:
5165 578 32 32
Tth111I GACn'n_nGTC
Cuts at: 3604 3604
Size: 5807
XbaI T'CTAG_A
Cuts at: 2838 2838
Size: 5807
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3302 3302
Size: 5807
XmaI C'CCGG_G
Cuts at: 2827 2827
Size: 5807
XmnI GAAnn'nnTTC
Cuts at: 1112 1263 1676 1814 1112
Size: 151 413 138 5105
Fragments arranged by size:
5105 413 151 138
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PflMI PfoI PmeI
PpiI PsiI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BbvCI BcgI BmgBI BmtI BsgI BsiWI
BsmBI BssHII BstZ17I Bsu36I ClaI EcoRV FseI FspAI
HpaI NdeI NheI NotI NruI PacI PasI PmlI
PpuMI PshAI PspOMI PspXI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI ZraI