Restriction map for
pF1KB8554
(Circular) (Six-base) MAPSORT of: pF1KB8554.seq Check: 5534 from: 1 to: 5837
pF1KB8554 5837 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2485 2485
Size: 5837
AccI GT'mk_AC
Cuts at: 2470 2875 2470
Size: 405 5432
Acc65I G'GTAC_C
Cuts at: 2853 2853
Size: 5837
AclI AA'CG_TT
Cuts at: 5677 5677
Size: 5837
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 1940 2190 2570 3662 4094 4841 5109 328
Size: 1612 250 380 1092 432 747 268 1056
Fragments arranged by size:
1612 1092 1056 747 432 380 268 250
AflIII A'CryG_T
Cuts at: 419 3189 4293 5270 419
Size: 2770 1104 977 986
Fragments arranged by size:
2770 1104 986 977
AgeI A'CCGG_T
Cuts at: 4225 4225
Size: 5837
AleI CACnn'nnGTG
Cuts at: 1906 1906
Size: 5837
AlwNI CAG_nnn'CTG
Cuts at: 3142 4709 3142
Size: 1567 4270
ApaLI G'TGCA_C
Cuts at: 4607 4607
Size: 5837
ApoI r'AATT_y
Cuts at: 889 1189 1203 1455 2841 5190 5446 889
Size: 300 14 252 1386 2349 256 1280
Fragments arranged by size:
2349 1386 1280 300 256 252 14
AseI AT'TA_AT
Cuts at: 20 475 20
Size: 455 5382
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5837
AvaI C'yCGr_G
Cuts at: 686 822 932 1280 2857 686
Size: 136 110 348 1577 3666
Fragments arranged by size:
3666 1577 348 136 110
AvrII C'CTAG_G
Cuts at: 2070 2070
Size: 5837
BamHI G'GATC_C
Cuts at: 176 1042 2256 2862 176
Size: 866 1214 606 3151
Fragments arranged by size:
3151 1214 866 606
BanI G'GyrC_C
Cuts at: 291 1863 2853 3267 3515 3550 291
Size: 1572 990 414 248 35 2578
Fragments arranged by size:
2578 1572 990 414 248 35
BanII G_rGCy'C
Cuts at: 1623 2851 3881 1623
Size: 1228 1030 3579
Fragments arranged by size:
3579 1228 1030
BbeI G_GCGC'C
Cuts at: 3271 3519 3271
Size: 248 5589
BbsI GAAGACnn'nnnn_
Cuts at: 1613 2058 1613
Size: 445 5392
BciVI GTATCCnnnnn_n'
Cuts at: 1378 2134 2362 2386 3735 4496 5837 1378
Size: 756 228 24 1349 761 1341 1378
Fragments arranged by size:
1378 1349 1341 761 756 228 24
BclI T'GATC_A
Cuts at: 2198 3357 2198
Size: 1159 4678
BfrBI ATG'CAT
Cuts at: 637 637
Size: 5837
BglII A'GATC_T
Cuts at: 818 1371 3352 5389 818
Size: 553 1981 2037 1266
Fragments arranged by size:
2037 1981 1266 553
BlpI GC'TnA_GC
Cuts at: 2949 2949
Size: 5837
Bme1580I G_kGCm'C
Cuts at: 3462 3555 4611 3462
Size: 93 1056 4688
Fragments arranged by size:
4688 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 754 2517 3049 3130 3465 5609 754
Size: 1763 532 81 335 2144 982
Fragments arranged by size:
2144 1763 982 532 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4204 4204
Size: 5837
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 905 1288 355
Size: 550 383 4904
Fragments arranged by size:
4904 550 383
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2311 3013 3892 4384 4682 2311
Size: 702 879 492 298 3466
Fragments arranged by size:
3466 879 702 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 262 1782 5541 262
Size: 1520 3759 558
Fragments arranged by size:
3759 1520 558
BsaAI yAC'GTr
Cuts at: 422 871 1977 3190 422
Size: 449 1106 1213 3069
Fragments arranged by size:
3069 1213 1106 449
BsaBI GATnn'nnATC
Cuts at: 6 2542 3351 5388 6
Size: 2536 809 2037 455
Fragments arranged by size:
2536 2037 809 455
BsaHI Gr'CG_yC
Cuts at: 554 1645 3268 3516 4218 5714 554
Size: 1091 1623 248 702 1496 677
Fragments arranged by size:
1623 1496 1091 702 677 248
BsaWI w'CCGG_w
Cuts at: 3026 3547 4225 4499 4646 5057 3026
Size: 521 678 274 147 411 3806
Fragments arranged by size:
3806 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2225 2255 2225
Size: 30 5807
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1087 1213 2682 1087
Size: 126 1469 4242
Fragments arranged by size:
4242 1469 126
BseYI C'CCAG_C
Cuts at: 3262 4020 4597 5093 3262
Size: 758 577 496 4006
Fragments arranged by size:
4006 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 487 1339 487
Size: 852 4985
BsiEI CG_ry'CG
Cuts at: 104 3379 3425 4633 104
Size: 3275 46 1208 1308
Fragments arranged by size:
3275 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 427 1982 2327 2827 2851 3629 3819 4611 427
Size: 1555 345 500 24 778 190 792 1653
Fragments arranged by size:
1653 1555 792 778 500 345 190 24
BsmI GAATG_Cn'
Cuts at: 1845 1845
Size: 5837
Bsp1286I G_dGCh'C
Cuts at: 427 1623 1982 2327 2827 2851 3462 3555 3629
Size: 1196 359 345 500 24 611 93 74
Cuts at: 3629 3819 3881 4611 427
Size: 190 62 730 1653
Fragments arranged by size:
1653 1196 730 611 500 359 345 190
93 74 62 24
BspEI T'CCGG_A
Cuts at: 5057 5057
Size: 5837
BspHI T'CATG_A
Cuts at: 5013 5013
Size: 5837
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2485 2887 3101 3403 3784 2485
Size: 402 214 302 381 4538
Fragments arranged by size:
4538 402 381 302 214
BsrBI CCG'CTC
Cuts at: 50 797 2819 4129 4183 5667 5832 50
Size: 747 2022 1310 54 1484 165 55
Fragments arranged by size:
2022 1484 1310 747 165 55 54
BsrDI GCAATG_nn'
Cuts at: 845 3749 845
Size: 2904 2933
BsrFI r'CCGG_y
Cuts at: 3835 4016 4225 3835
Size: 181 209 5447
Fragments arranged by size:
5447 209 181
BsrGI T'GTAC_A
Cuts at: 2540 2540
Size: 5837
BssSI C'ACGA_G
Cuts at: 4108 4466 4108
Size: 358 5479
BstAPI GCAn_nnn'nTGC
Cuts at: 1362 1811 3214 1362
Size: 449 1403 3985
Fragments arranged by size:
3985 1403 449
BstBI TT'CG_AA
Cuts at: 4198 4198
Size: 5837
BstXI CCAn_nnnn'nTGG
Cuts at: 231 2230 231
Size: 1999 3838
BstYI r'GATC_y
Cuts at: 1 176 516 818 1042 1371 2256 2301 2862
Size: 175 340 302 224 329 885 45 561
Cuts at: 2862 3329 3352 3687 3933 4945 5031 5043 5389
Size: 467 23 335 246 1012 86 12 346
Cuts at: 5389 1
Size: 449
Fragments arranged by size:
1012 885 561 467 449 346 340 335
329 302 246 224 175 86 45 23
12
BstZ17I GTA'TAC
Cuts at: 2471 2471
Size: 5837
BtgI C'CryG_G
Cuts at: 107 722 1340 107
Size: 615 618 4604
Fragments arranged by size:
4604 618 615
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1878 3787 3968 4131 1878
Size: 1909 181 163 3584
Fragments arranged by size:
3584 1909 181 163
BtsI GCAGTG_nn'
Cuts at: 944 1473 1621 3080 944
Size: 529 148 1459 3701
Fragments arranged by size:
3701 1459 529 148
ClaI AT'CG_AT
Cuts at: 1134 1134
Size: 5837
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1887 1922 1887
Size: 35 5802
DraI TTT'AAA
Cuts at: 2836 2836
Size: 5837
DrdI GACnn_nn'nnGTC
Cuts at: 3543 4401 3543
Size: 858 4979
EaeI y'GGCC_r
Cuts at: 1572 3422 3596 3987 4014 5359 1572
Size: 1850 174 391 27 1345 2050
Fragments arranged by size:
2050 1850 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3422 3422
Size: 5837
EarI CTCTTCn'nnn_
Cuts at: 265 3860 4070 265
Size: 3595 210 2032
Fragments arranged by size:
3595 2032 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 686 4045 4355 4501 5647 686
Size: 3359 310 146 1146 876
Fragments arranged by size:
3359 1146 876 310 146
EcoICRI GAG'CTC
Cuts at: 2849 2849
Size: 5837
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 355 905 1288 1940 2190 2570 3662 4094
Size: 27 550 383 652 250 380 1092 432
Cuts at: 4094 4841 5109 328
Size: 747 268 1056
Fragments arranged by size:
1092 1056 747 652 550 432 383 380
268 250 27
EcoO109I rG'GnC_Cy
Cuts at: 372 1701 2976 372
Size: 1329 1275 3233
Fragments arranged by size:
3233 1329 1275
EcoRI G'AATT_C
Cuts at: 1189 2841 1189
Size: 1652 4185
EcoRV GAT'ATC
Cuts at: 1524 1524
Size: 5837
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1450 2688 3856 1450
Size: 1238 1168 3431
Fragments arranged by size:
3431 1238 1168
FspI TGC'GCA
Cuts at: 3618 3618
Size: 5837
HaeII r_GCGC'y
Cuts at: 2167 3271 3519 4541 5520 2167
Size: 1104 248 1022 979 2484
Fragments arranged by size:
2484 1104 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 409 441 667 699 1744 1776 2225 2257 2286
Size: 32 226 32 1045 32 449 32 29
Cuts at: 2286 2318 2360 2392 3679 3709 3711 3741 409
Size: 32 42 32 1287 30 2 30 2505
Fragments arranged by size:
2505 1287 1045 449 226 42 32 32
32 32 32 32 30 30 29 2
HincII GTy'rAC
Cuts at: 2876 2876
Size: 5837
KasI G'GCGC_C
Cuts at: 3267 3515 3267
Size: 248 5589
KpnI G_GTAC'C
Cuts at: 2857 2857
Size: 5837
MfeI C'AATT_G
Cuts at: 353 1118 353
Size: 765 5072
MluI A'CGCG_T
Cuts at: 5270 5270
Size: 5837
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 413 2082 4508 4692 413
Size: 1669 2426 184 1558
Fragments arranged by size:
2426 1669 1558 184
MscI TGG'CCA
Cuts at: 3598 3598
Size: 5837
MslI CAynn'nnrTG
Cuts at: 187 229 747 1906 3826 3953 187
Size: 42 518 1159 1920 127 2071
Fragments arranged by size:
2071 1920 1159 518 127 42
MspA1I CmG'CkG
Cuts at: 2193 2292 2692 2948 3262 3622 4635 4880 2193
Size: 99 400 256 314 360 1013 245 3150
Fragments arranged by size:
3150 1013 400 360 314 256 245 99
NaeI GCC'GGC
Cuts at: 4018 4018
Size: 5837
NarI GG'CG_CC
Cuts at: 3268 3516 3268
Size: 248 5589
NcoI C'CATG_G
Cuts at: 107 722 1340 107
Size: 615 618 4604
Fragments arranged by size:
4604 618 615
NdeI CA'TA_TG
Cuts at: 2047 2047
Size: 5837
NgoMIV G'CCGG_C
Cuts at: 4016 4016
Size: 5837
NsiI A_TGCA'T
Cuts at: 639 639
Size: 5837
NspI r_CATG'y
Cuts at: 1614 2085 2604 2890 3390 4297 1614
Size: 471 519 286 500 907 3154
Fragments arranged by size:
3154 907 519 500 471 286
PasI CC'CwG_GG
Cuts at: 1853 1853
Size: 5837
PciI A'CATG_T
Cuts at: 4293 4293
Size: 5837
PflMI CCAn_nnn'nTGG
Cuts at: 723 883 723
Size: 160 5677
PfoI T'CCnGG_A
Cuts at: 1509 1509
Size: 5837
PmeI GTTT'AAAC
Cuts at: 2836 2836
Size: 5837
PmlI CAC'GTG
Cuts at: 422 871 422
Size: 449 5388
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4976 5008 4976
Size: 32 5805
PpuMI rG'GwC_Cy
Cuts at: 372 1701 372
Size: 1329 4508
PshAI GACnn'nnGTC
Cuts at: 1921 1921
Size: 5837
PsiI TTA'TAA
Cuts at: 2629 2629
Size: 5837
PspXI vC'TCGA_Gb
Cuts at: 932 932
Size: 5837
PstI C_TGCA'G
Cuts at: 570 1657 2884 3569 570
Size: 1087 1227 685 2838
Fragments arranged by size:
2838 1227 1087 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5837
PvuII CAG'CTG
Cuts at: 2193 2692 3262 3622 2193
Size: 499 570 360 4408
Fragments arranged by size:
4408 570 499 360
RsrII CG'GwC_CG
Cuts at: 4032 4032
Size: 5837
SacI G_AGCT'C
Cuts at: 2851 2851
Size: 5837
SalI G'TCGA_C
Cuts at: 2874 2874
Size: 5837
SanDI GG'GwC_CC
Cuts at: 1701 1701
Size: 5837
SapI GCTCTTCn'nnn_
Cuts at: 3860 4070 3860
Size: 210 5627
SbfI CC_TGCA'GG
Cuts at: 2884 2884
Size: 5837
ScaI AGT'ACT
Cuts at: 5354 5354
Size: 5837
SexAI A'CCwGG_T
Cuts at: 667 1745 667
Size: 1078 4759
SfcI C'TryA_G
Cuts at: 33 566 1653 2795 2880 3565 4558 4749 33
Size: 533 1087 1142 85 685 993 191 1121
Fragments arranged by size:
1142 1121 1087 993 685 533 191 85
SfoI GGC'GCC
Cuts at: 3269 3517 3269
Size: 248 5589
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5837
SmaI CCC'GGG
Cuts at: 2859 2859
Size: 5837
SmlI C'TyrA_G
Cuts at: 686 822 932 2326 2992 3907 4399 4661 686
Size: 136 110 1394 666 915 492 262 1862
Fragments arranged by size:
1862 1394 915 666 492 262 136 110
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5837
SphI G_CATG'C
Cuts at: 2604 2890 3390 2604
Size: 286 500 5051
Fragments arranged by size:
5051 500 286
SspI AAT'ATT
Cuts at: 1883 2135 1883
Size: 252 5585
StuI AGG'CCT
Cuts at: 808 808
Size: 5837
StyI C'CwwG_G
Cuts at: 107 722 1340 2070 2229 2615 2971 3325 107
Size: 615 618 730 159 386 356 354 2619
Fragments arranged by size:
2619 730 618 615 386 356 354 159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 391 537 829 3420 3553 4012 4221 391
Size: 146 292 2591 133 459 209 2007
Fragments arranged by size:
2591 2007 459 292 209 146 133
TatI w'GTAC_w
Cuts at: 1559 2395 2540 5352 1559
Size: 836 145 2812 2044
Fragments arranged by size:
2812 2044 836 145
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 487 519 774 806 848 880 5354 5386 487
Size: 32 255 32 42 32 4474 32 938
Fragments arranged by size:
4474 938 255 42 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3634 3634
Size: 5837
XbaI T'CTAG_A
Cuts at: 2868 2868
Size: 5837
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 396 1833 3332 396
Size: 1437 1499 2901
Fragments arranged by size:
2901 1499 1437
XhoI C'TCGA_G
Cuts at: 686 822 932 686
Size: 136 110 5591
Fragments arranged by size:
5591 136 110
XmaI C'CCGG_G
Cuts at: 2857 2857
Size: 5837
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
BaeI BbvCI BcgI BglI BmgBI BmtI Bpu10I BsiWI
BsmBI BssHII BstEII Bsu36I DraIII EcoNI FseI FspAI
HindIII HpaI NheI NotI NruI PacI PspOMI PsrI
SacII SfiI SgrAI SnaBI SrfI SwaI XmnI ZraI