Restriction map for
pF1KB8562
(Circular) (Six-base) MAPSORT of: pF1KB8562.seq Check: 3038 from: 1 to: 5846
pF1KB8562 5846 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AccI GT'mk_AC
Cuts at: 2884 2884
Size: 5846
Acc65I G'GTAC_C
Cuts at: 265 1846 2862 265
Size: 1581 1016 3249
Fragments arranged by size:
3249 1581 1016
AclI AA'CG_TT
Cuts at: 5686 5686
Size: 5846
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2724 2740 3671 4103 4850 5118 2724
Size: 16 931 432 747 268 3452
Fragments arranged by size:
3452 931 747 432 268 16
AflIII A'CryG_T
Cuts at: 1775 3198 4302 5279 1775
Size: 1423 1104 977 2342
Fragments arranged by size:
2342 1423 1104 977
AgeI A'CCGG_T
Cuts at: 4234 4234
Size: 5846
AhdI GACnn_n'nnGTC
Cuts at: 1553 2401 1553
Size: 848 4998
AleI CACnn'nnGTG
Cuts at: 790 1441 2290 790
Size: 651 849 4346
Fragments arranged by size:
4346 849 651
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1081 1081
Size: 5846
AlwNI CAG_nnn'CTG
Cuts at: 297 324 669 1101 1323 1482 2353 2763 3151
Size: 27 345 432 222 159 871 410 388
Cuts at: 3151 4718 297
Size: 1567 1425
Fragments arranged by size:
1567 1425 871 432 410 388 345 222
159 27
ApaI G_GGCC'C
Cuts at: 576 1210 1804 576
Size: 634 594 4618
Fragments arranged by size:
4618 634 594
ApaLI G'TGCA_C
Cuts at: 4616 4616
Size: 5846
ApoI r'AATT_y
Cuts at: 752 2850 5199 5455 752
Size: 2098 2349 256 1143
Fragments arranged by size:
2349 2098 1143 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5846
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5846
AvaI C'yCGr_G
Cuts at: 187 261 2042 2866 187
Size: 74 1781 824 3167
Fragments arranged by size:
3167 1781 824 74
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 249 282 2542 2575 249
Size: 33 2260 33 3520
Fragments arranged by size:
3520 2260 33 33
BamHI G'GATC_C
Cuts at: 654 2871 654
Size: 2217 3629
BanI G'GyrC_C
Cuts at: 265 885 1113 1268 1785 1846 2007 2862 3276
Size: 620 228 155 517 61 161 855 414
Cuts at: 3276 3524 3559 265
Size: 248 35 2552
Fragments arranged by size:
2552 855 620 517 414 248 228 161
155 61 35
BanII G_rGCy'C
Cuts at: 576 1210 1476 1804 2206 2441 2860 3890 576
Size: 634 266 328 402 235 419 1030 2532
Fragments arranged by size:
2532 1030 634 419 402 328 266 235
BbeI G_GCGC'C
Cuts at: 1272 3280 3528 1272
Size: 2008 248 3590
Fragments arranged by size:
3590 2008 248
BbsI GAAGACnn'nnnn_
Cuts at: 140 179 438 539 1765 2121 2599 140
Size: 39 259 101 1226 356 478 3387
Fragments arranged by size:
3387 1226 478 356 259 101 39
BbvCI CC'TCA_GC
Cuts at: 2037 2037
Size: 5846
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1440 1474 1440
Size: 34 5812
BciVI GTATCCnnnnn_n'
Cuts at: 1977 3744 4505 5846 1977
Size: 1767 761 1341 1977
Fragments arranged by size:
1977 1767 1341 761
BclI T'GATC_A
Cuts at: 1727 1952 3366 1727
Size: 225 1414 4207
Fragments arranged by size:
4207 1414 225
BfrBI ATG'CAT
Cuts at: 1306 1306
Size: 5846
BglI GCCn_nnn'nGGC
Cuts at: 1006 2677 2715 1006
Size: 1671 38 4137
Fragments arranged by size:
4137 1671 38
BglII A'GATC_T
Cuts at: 1673 1733 3361 5398 1673
Size: 60 1628 2037 2121
Fragments arranged by size:
2121 2037 1628 60
BlpI GC'TnA_GC
Cuts at: 1475 2506 2958 1475
Size: 1031 452 4363
Fragments arranged by size:
4363 1031 452
Bme1580I G_kGCm'C
Cuts at: 576 730 740 1210 1790 1804 2576 3471 3564
Size: 154 10 470 580 14 772 895 93
Cuts at: 3564 4620 576
Size: 1056 1802
Fragments arranged by size:
1802 1056 895 772 580 470 154 93
14 10
BmgBI CAC'GTC
Cuts at: 2501 2501
Size: 5846
BmrI ACTGGGnnnn_n'
Cuts at: 2074 3058 3139 3474 5618 2074
Size: 984 81 335 2144 2302
Fragments arranged by size:
2302 2144 984 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1695 1734 4213 1695
Size: 39 2479 3328
Fragments arranged by size:
3328 2479 39
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 379 441 515 620 628 712 838 886
Size: 204 62 74 105 8 84 126 48
Cuts at: 886 1158 2167 175
Size: 272 1009 3854
Fragments arranged by size:
3854 1009 272 204 126 105 84 74
62 48 8
Bpu10I CC'TnA_GC
Cuts at: 2037 2183 2037
Size: 146 5700
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3022 3901 4393 4691 3022
Size: 879 492 298 4177
Fragments arranged by size:
4177 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 475 1540 5550 475
Size: 1065 4010 771
Fragments arranged by size:
4010 1065 771
BsaAI yAC'GTr
Cuts at: 3199 3199
Size: 5846
BsaBI GATnn'nnATC
Cuts at: 6 1732 3360 5397 6
Size: 1726 1628 2037 455
Fragments arranged by size:
2037 1726 1628 455
BsaHI Gr'CG_yC
Cuts at: 1269 3277 3525 4227 5723 1269
Size: 2008 248 702 1496 1392
Fragments arranged by size:
2008 1496 1392 702 248
BsaWI w'CCGG_w
Cuts at: 3035 3556 4234 4508 4655 5066 3035
Size: 521 678 274 147 411 3815
Fragments arranged by size:
3815 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2743 2773 2743
Size: 30 5816
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 160 217 376 574 675 1813 2025 2812
Size: 33 57 159 198 101 1138 212 787
Cuts at: 2812 127
Size: 3161
Fragments arranged by size:
3161 1138 787 212 198 159 101 57
33
BseYI C'CCAG_C
Cuts at: 347 831 912 944 1467 2048 3271 4029 4606
Size: 484 81 32 523 581 1223 758 577
Cuts at: 4606 5102 347
Size: 496 1091
Fragments arranged by size:
1223 1091 758 581 577 523 496 484
81 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1015 1141 1337 1465 1015
Size: 126 196 128 5396
Fragments arranged by size:
5396 196 128 126
BsiEI CG_ry'CG
Cuts at: 104 3388 3434 4642 104
Size: 3284 46 1208 1308
Fragments arranged by size:
3284 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1838 2860 3638 3828 4620 1838
Size: 1022 778 190 792 3064
Fragments arranged by size:
3064 1022 792 778 190
BsmI GAATG_Cn'
Cuts at: 1306 1451 1306
Size: 145 5701
Bsp1286I G_dGCh'C
Cuts at: 576 730 740 1210 1476 1790 1804 1838 2206
Size: 154 10 470 266 314 14 34 368
Cuts at: 2206 2441 2576 2860 3471 3564 3638 3828 3890
Size: 235 135 284 611 93 74 190 62
Cuts at: 3890 4620 576
Size: 730 1802
Fragments arranged by size:
1802 730 611 470 368 314 284 266
235 190 154 135 93 74 62 34
14 10
BspEI T'CCGG_A
Cuts at: 5066 5066
Size: 5846
BspHI T'CATG_A
Cuts at: 5022 5022
Size: 5846
BspMI ACCTGCnnnn'nnnn_
Cuts at: 482 2231 2896 3110 3412 3793 482
Size: 1749 665 214 302 381 2535
Fragments arranged by size:
2535 1749 665 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2733 4138 4192 5676 5841 50
Size: 2683 1405 54 1484 165 55
Fragments arranged by size:
2683 1484 1405 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3758 3758
Size: 5846
BsrFI r'CCGG_y
Cuts at: 207 825 882 1843 2667 3844 4025 4234 207
Size: 618 57 961 824 1177 181 209 1819
Fragments arranged by size:
1819 1177 961 824 618 209 181 57
BssHII G'CGCG_C
Cuts at: 343 343
Size: 5846
BssSI C'ACGA_G
Cuts at: 4117 4475 4117
Size: 358 5488
BstAPI GCAn_nnn'nTGC
Cuts at: 828 1101 3223 828
Size: 273 2122 3451
Fragments arranged by size:
3451 2122 273
BstBI TT'CG_AA
Cuts at: 1137 4207 1137
Size: 3070 2776
BstXI CCAn_nnnn'nTGG
Cuts at: 2433 2838 2433
Size: 405 5441
BstYI r'GATC_y
Cuts at: 1 539 654 1673 1733 2363 2871 3338 3361
Size: 538 115 1019 60 630 508 467 23
Cuts at: 3361 3696 3942 4954 5040 5052 5398 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1019 1012 630 538 508 467 449 346
335 246 115 86 60 23 12
Bsu36I CC'TnA_GG
Cuts at: 548 920 1796 548
Size: 372 876 4598
Fragments arranged by size:
4598 876 372
BtgI C'CryG_G
Cuts at: 107 107
Size: 5846
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3796 3977 4140 3796
Size: 181 163 5502
Fragments arranged by size:
5502 181 163
BtsI GCAGTG_nn'
Cuts at: 1099 1436 1448 2648 3089 1099
Size: 337 12 1200 441 3856
Fragments arranged by size:
3856 1200 441 337 12
ClaI AT'CG_AT
Cuts at: 935 935
Size: 5846
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2051 2086 2151 2186 2051
Size: 35 65 35 5711
Fragments arranged by size:
5711 65 35 35
DraI TTT'AAA
Cuts at: 2845 2845
Size: 5846
DrdI GACnn_nn'nnGTC
Cuts at: 1933 3552 4410 1933
Size: 1619 858 3369
Fragments arranged by size:
3369 1619 858
EaeI y'GGCC_r
Cuts at: 500 951 1841 2252 2828 3431 3605 3996 4023
Size: 451 890 411 576 603 174 391 27
Cuts at: 4023 5368 500
Size: 1345 978
Fragments arranged by size:
1345 978 890 603 576 451 411 391
174 27
EagI C'GGCC_G
Cuts at: 3431 3431
Size: 5846
EarI CTCTTCn'nnn_
Cuts at: 709 1246 1462 3869 4079 709
Size: 537 216 2407 210 2476
Fragments arranged by size:
2476 2407 537 216 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2097 4054 4364 4510 5656 2097
Size: 1957 310 146 1146 2287
Fragments arranged by size:
2287 1957 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2858 2858
Size: 5846
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 379 441 515 620 628 712 838 886
Size: 204 62 74 105 8 84 126 48
Cuts at: 886 1158 2167 2724 2740 3671 4103 4850 5118
Size: 272 1009 557 16 931 432 747 268
Cuts at: 5118 175
Size: 903
Fragments arranged by size:
1009 931 903 747 557 432 272 268
204 126 105 84 74 62 48 16
8
EcoO109I rG'GnC_Cy
Cuts at: 383 573 612 916 924 1188 1206 1248 1320
Size: 190 39 304 8 264 18 42 72
Cuts at: 1320 1504 1800 1801 1935 2985 383
Size: 184 296 1 134 1050 3244
Fragments arranged by size:
3244 1050 304 296 264 190 184 134
72 42 39 18 8 1
EcoRI G'AATT_C
Cuts at: 752 2850 752
Size: 2098 3748
EcoRV GAT'ATC
Cuts at: 1263 1263
Size: 5846
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2805 3865 2805
Size: 1060 4786
FspI TGC'GCA
Cuts at: 1873 3627 1873
Size: 1754 4092
HaeII r_GCGC'y
Cuts at: 1146 1272 3280 3528 4550 5529 1146
Size: 126 2008 248 1022 979 1463
Fragments arranged by size:
2008 1463 1022 979 248 126
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 531 563 1072 1104 1539 1571 2387 2419 3688
Size: 32 509 32 435 32 816 32 1269
Cuts at: 3688 3718 3720 3750 531
Size: 30 2 30 2627
Fragments arranged by size:
2627 1269 816 509 435 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2885 2885
Size: 5846
KasI G'GCGC_C
Cuts at: 1268 3276 3524 1268
Size: 2008 248 3590
Fragments arranged by size:
3590 2008 248
KpnI G_GTAC'C
Cuts at: 269 1850 2866 269
Size: 1581 1016 3249
Fragments arranged by size:
3249 1581 1016
MluI A'CGCG_T
Cuts at: 5279 5279
Size: 5846
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1400 2054 4517 4701 1400
Size: 654 2463 184 2545
Fragments arranged by size:
2545 2463 654 184
MscI TGG'CCA
Cuts at: 502 2830 3607 502
Size: 2328 777 2741
Fragments arranged by size:
2741 2328 777
MslI CAynn'nnrTG
Cuts at: 790 970 1441 2092 2290 2326 2431 2567 2836
Size: 180 471 651 198 36 105 136 269
Cuts at: 2836 3835 3962 790
Size: 999 127 2674
Fragments arranged by size:
2674 999 651 471 269 198 180 136
127 105 36
MspA1I CmG'CkG
Cuts at: 859 2385 2510 2712 2957 3271 3631 4644 4889
Size: 1526 125 202 245 314 360 1013 245
Cuts at: 4889 859
Size: 1816
Fragments arranged by size:
1816 1526 1013 360 314 245 245 202
125
NaeI GCC'GGC
Cuts at: 209 2669 4027 209
Size: 2460 1358 2028
Fragments arranged by size:
2460 2028 1358
NarI GG'CG_CC
Cuts at: 1269 3277 3525 1269
Size: 2008 248 3590
Fragments arranged by size:
3590 2008 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5846
NgoMIV G'CCGG_C
Cuts at: 207 2667 4025 207
Size: 2460 1358 2028
Fragments arranged by size:
2460 2028 1358
NsiI A_TGCA'T
Cuts at: 1308 1308
Size: 5846
NspI r_CATG'y
Cuts at: 2607 2899 3399 4306 2607
Size: 292 500 907 4147
Fragments arranged by size:
4147 907 500 292
PasI CC'CwG_GG
Cuts at: 615 2347 615
Size: 1732 4114
PciI A'CATG_T
Cuts at: 4302 4302
Size: 5846
PflMI CCAn_nnn'nTGG
Cuts at: 1323 2064 1323
Size: 741 5105
PfoI T'CCnGG_A
Cuts at: 815 1049 1739 2144 2366 815
Size: 234 690 405 222 4295
Fragments arranged by size:
4295 690 405 234 222
PmeI GTTT'AAAC
Cuts at: 2845 2845
Size: 5846
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 970 1002 4985 5017 970
Size: 32 3983 32 1799
Fragments arranged by size:
3983 1799 32 32
PpuMI rG'GwC_Cy
Cuts at: 916 1320 1504 1935 916
Size: 404 184 431 4827
Fragments arranged by size:
4827 431 404 184
PsiI TTA'TAA
Cuts at: 1766 1766
Size: 5846
PspOMI G'GGCC_C
Cuts at: 572 1206 1800 572
Size: 634 594 4618
Fragments arranged by size:
4618 634 594
PstI C_TGCA'G
Cuts at: 122 293 636 851 1060 1226 1443 2358 2893
Size: 171 343 215 209 166 217 915 535
Cuts at: 2893 3578 122
Size: 685 2390
Fragments arranged by size:
2390 915 685 535 343 217 215 209
171 166
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5846
PvuII CAG'CTG
Cuts at: 3271 3631 3271
Size: 360 5486
RsrII CG'GwC_CG
Cuts at: 4041 4041
Size: 5846
SacI G_AGCT'C
Cuts at: 2860 2860
Size: 5846
SalI G'TCGA_C
Cuts at: 2883 2883
Size: 5846
SanDI GG'GwC_CC
Cuts at: 1935 1935
Size: 5846
SapI GCTCTTCn'nnn_
Cuts at: 709 1246 3869 4079 709
Size: 537 2623 210 2476
Fragments arranged by size:
2623 2476 537 210
SbfI CC_TGCA'GG
Cuts at: 2893 2893
Size: 5846
ScaI AGT'ACT
Cuts at: 5363 5363
Size: 5846
SfcI C'TryA_G
Cuts at: 33 118 289 632 847 1056 1222 1439 1753
Size: 85 171 343 215 209 166 217 314
Cuts at: 1753 2354 2889 3574 4567 4758 33
Size: 601 535 685 993 191 1121
Fragments arranged by size:
1121 993 685 601 535 343 314 217
215 209 191 171 166 85
SfiI GGCCn_nnn'nGGCC
Cuts at: 2677 2677
Size: 5846
SfoI GGC'GCC
Cuts at: 1270 3278 3526 1270
Size: 2008 248 3590
Fragments arranged by size:
3590 2008 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5846
SmaI CCC'GGG
Cuts at: 263 2044 2868 263
Size: 1781 824 3241
Fragments arranged by size:
3241 1781 824
SmlI C'TyrA_G
Cuts at: 3001 3916 4408 4670 3001
Size: 915 492 262 4177
Fragments arranged by size:
4177 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5846
SphI G_CATG'C
Cuts at: 2607 2899 3399 2607
Size: 292 500 5054
Fragments arranged by size:
5054 500 292
StyI C'CwwG_G
Cuts at: 107 452 2780 2980 3334 107
Size: 345 2328 200 354 2619
Fragments arranged by size:
2619 2328 354 345 200
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 339 569 958 1763 2008 2075 2233 2320 2568
Size: 230 389 805 245 67 158 87 248
Cuts at: 2568 2579 2640 3429 3562 4021 4230 339
Size: 11 61 789 133 459 209 1955
Fragments arranged by size:
1955 805 789 459 389 248 245 230
209 158 133 87 67 61 11
TatI w'GTAC_w
Cuts at: 1562 2466 5361 1562
Size: 904 2895 2047
Fragments arranged by size:
2895 2047 904
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2743 2775 5363 5395 2743
Size: 32 2588 32 3194
Fragments arranged by size:
3194 2588 32 32
Tth111I GACn'n_nGTC
Cuts at: 3643 3643
Size: 5846
XbaI T'CTAG_A
Cuts at: 1557 2877 1557
Size: 1320 4526
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1953 3341 1953
Size: 1388 4458
XmaI C'CCGG_G
Cuts at: 261 2042 2866 261
Size: 1781 824 3241
Fragments arranged by size:
3241 1781 824
XmnI GAAnn'nnTTC
Cuts at: 764 764
Size: 5846
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AloI AscI AvrII BmtI
BsiWI BsmBI BsrGI BstEII BstZ17I DraIII EcoNI FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
PacI PmlI PshAI PspXI PsrI SacII SexAI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI