Restriction map for
pF1KB8564
(Circular) (Six-base) MAPSORT of: pF1KB8564.seq Check: 3153 from: 1 to: 5846
pF1KB8564 5846 bp
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2536 2536
Size: 5846
AatII G_ACGT'C
Cuts at: 467 2775 467
Size: 2308 3538
AccI GT'mk_AC
Cuts at: 1157 2884 1157
Size: 1727 4119
Acc65I G'GTAC_C
Cuts at: 1425 1915 2321 2862 1425
Size: 490 406 541 4409
Fragments arranged by size:
4409 541 490 406
AclI AA'CG_TT
Cuts at: 5686 5686
Size: 5846
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 884 1961 3671 4103 4850 5118 884
Size: 1077 1710 432 747 268 1612
Fragments arranged by size:
1710 1612 1077 747 432 268
AfeI AGC'GCT
Cuts at: 1069 1069
Size: 5846
AflIII A'CryG_T
Cuts at: 3198 4302 5279 3198
Size: 1104 977 3765
Fragments arranged by size:
3765 1104 977
AgeI A'CCGG_T
Cuts at: 419 2318 2633 4234 419
Size: 1899 315 1601 2031
Fragments arranged by size:
2031 1899 1601 315
AleI CACnn'nnGTG
Cuts at: 2078 2078
Size: 5846
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 701 734 1676 701
Size: 33 942 4871
Fragments arranged by size:
4871 942 33
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1747 1779 1747
Size: 32 5814
AlwNI CAG_nnn'CTG
Cuts at: 1228 2069 3151 4718 1228
Size: 841 1082 1567 2356
Fragments arranged by size:
2356 1567 1082 841
ApaI G_GGCC'C
Cuts at: 633 1579 1908 633
Size: 946 329 4571
Fragments arranged by size:
4571 946 329
ApaLI G'TGCA_C
Cuts at: 4616 4616
Size: 5846
ApoI r'AATT_y
Cuts at: 1405 2850 5199 5455 1405
Size: 1445 2349 256 1796
Fragments arranged by size:
2349 1796 1445 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5846
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5846
AvaI C'yCGr_G
Cuts at: 356 802 1572 1841 2293 2738 2866 356
Size: 446 770 269 452 445 128 3336
Fragments arranged by size:
3336 770 452 446 445 269 128
AvrII C'CTAG_G
Cuts at: 1390 1390
Size: 5846
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 489 522 489
Size: 33 5813
BamHI G'GATC_C
Cuts at: 2871 2871
Size: 5846
BanI G'GyrC_C
Cuts at: 259 1425 1632 1876 1915 2197 2321 2636 2862
Size: 1166 207 244 39 282 124 315 226
Cuts at: 2862 3276 3524 3559 259
Size: 414 248 35 2546
Fragments arranged by size:
2546 1166 414 315 282 248 244 226
207 124 39 35
BanII G_rGCy'C
Cuts at: 328 601 633 660 1565 1579 1908 2745 2860
Size: 273 32 27 905 14 329 837 115
Cuts at: 2860 3890 328
Size: 1030 2284
Fragments arranged by size:
2284 1030 905 837 329 273 115 32
27 14
BbeI G_GCGC'C
Cuts at: 263 1636 2201 3280 3528 263
Size: 1373 565 1079 248 2581
Fragments arranged by size:
2581 1373 1079 565 248
BbsI GAAGACnn'nnnn_
Cuts at: 872 1379 2736 872
Size: 507 1357 3982
Fragments arranged by size:
3982 1357 507
BciVI GTATCCnnnnn_n'
Cuts at: 761 3744 4505 5846 761
Size: 2983 761 1341 761
Fragments arranged by size:
2983 1341 761 761
BclI T'GATC_A
Cuts at: 3366 3366
Size: 5846
BglI GCCn_nnn'nGGC
Cuts at: 637 1903 637
Size: 1266 4580
BglII A'GATC_T
Cuts at: 2711 3361 5398 2711
Size: 650 2037 3159
Fragments arranged by size:
3159 2037 650
BlpI GC'TnA_GC
Cuts at: 478 1564 2958 478
Size: 1086 1394 3366
Fragments arranged by size:
3366 1394 1086
Bme1580I G_kGCm'C
Cuts at: 633 648 1579 1881 1908 2504 2726 3471 3564
Size: 15 931 302 27 596 222 745 93
Cuts at: 3564 4620 633
Size: 1056 1859
Fragments arranged by size:
1859 1056 931 745 596 302 222 93
27 15
BmgBI CAC'GTC
Cuts at: 282 2499 282
Size: 2217 3629
BmrI ACTGGGnnnn_n'
Cuts at: 2244 2797 3058 3139 3474 5618 2244
Size: 553 261 81 335 2144 2472
Fragments arranged by size:
2472 2144 553 335 261 81
BmtI G_CTAG'C
Cuts at: 1027 1027
Size: 5846
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1953 4213 1953
Size: 2260 3586
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 884 1288 1352 1948 2468 884
Size: 404 64 596 520 4262
Fragments arranged by size:
4262 596 520 404 64
Bpu10I CC'TnA_GC
Cuts at: 1823 1823
Size: 5846
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2564 3022 3901 4393 4691 2564
Size: 458 879 492 298 3719
Fragments arranged by size:
3719 879 492 458 298
BsaI GGTCTCn'nnnn_
Cuts at: 1255 2239 5550 1255
Size: 984 3311 1551
Fragments arranged by size:
3311 1551 984
BsaAI yAC'GTr
Cuts at: 3199 3199
Size: 5846
BsaBI GATnn'nnATC
Cuts at: 6 3360 5397 6
Size: 3354 2037 455
Fragments arranged by size:
3354 2037 455
BsaHI Gr'CG_yC
Cuts at: 260 464 1633 2186 2198 2390 2772 3277 3525
Size: 204 1169 553 12 192 382 505 248
Cuts at: 3525 4227 5723 260
Size: 702 1496 383
Fragments arranged by size:
1496 1169 702 553 505 383 382 248
204 192 12
BsaWI w'CCGG_w
Cuts at: 419 809 2318 2562 2633 3035 3556 4234 4508
Size: 390 1509 244 71 402 521 678 274
Cuts at: 4508 4655 5066 419
Size: 147 411 1199
Fragments arranged by size:
1509 1199 678 521 411 402 390 274
244 147 71
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 383 413 1198 1228 2018 2048 2320 2350 383
Size: 30 785 30 790 30 272 30 3879
Fragments arranged by size:
3879 790 785 272 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1364 1519 1534 1537 1859 1870 2110 2113 2251
Size: 155 15 3 322 11 240 3 138
Cuts at: 2251 2770 1364
Size: 519 4440
Fragments arranged by size:
4440 519 322 240 155 138 15 11
3 3
BseYI C'CCAG_C
Cuts at: 113 321 447 1060 1178 1209 1221 1377 1555
Size: 208 126 613 118 31 12 156 178
Cuts at: 1555 1965 1988 2062 2180 2231 2654 3271 4029
Size: 410 23 74 118 51 423 617 758
Cuts at: 4029 4606 5102 113
Size: 577 496 857
Fragments arranged by size:
857 758 617 613 577 496 423 410
208 178 156 126 118 118 74 51
31 23 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 971 2002 2731 971
Size: 1031 729 4086
Fragments arranged by size:
4086 1031 729
BsiEI CG_ry'CG
Cuts at: 104 2592 2694 3388 3434 4642 104
Size: 2488 102 694 46 1208 1308
Fragments arranged by size:
2488 1308 1208 694 102 46
BsiHKAI G_wGCw'C
Cuts at: 254 1565 2745 2860 3638 3828 4620 254
Size: 1311 1180 115 778 190 792 1480
Fragments arranged by size:
1480 1311 1180 792 778 190 115
BsmBI CGTCTCn'nnnn_
Cuts at: 471 471
Size: 5846
Bsp1286I G_dGCh'C
Cuts at: 254 328 601 633 648 660 1565 1579 1881
Size: 74 273 32 15 12 905 14 302
Cuts at: 1881 1908 2504 2726 2745 2860 3471 3564 3638
Size: 27 596 222 19 115 611 93 74
Cuts at: 3638 3828 3890 4620 254
Size: 190 62 730 1480
Fragments arranged by size:
1480 905 730 611 596 302 273 222
190 115 93 74 74 62 32 27
19 15 14 12
BspEI T'CCGG_A
Cuts at: 809 5066 809
Size: 4257 1589
BspHI T'CATG_A
Cuts at: 5022 5022
Size: 5846
BspMI ACCTGCnnnn'nnnn_
Cuts at: 299 2536 2742 2896 3110 3412 3793 299
Size: 2237 206 154 214 302 381 2352
Fragments arranged by size:
2352 2237 381 302 214 206 154
BsrBI CCG'CTC
Cuts at: 50 945 4138 4192 5676 5841 50
Size: 895 3193 54 1484 165 55
Fragments arranged by size:
3193 1484 895 165 55 54
BsrDI GCAATG_nn'
Cuts at: 718 3758 718
Size: 3040 2806
BsrFI r'CCGG_y
Cuts at: 419 2318 2633 3844 4025 4234 419
Size: 1899 315 1211 181 209 2031
Fragments arranged by size:
2031 1899 1211 315 209 181
BssHII G'CGCG_C
Cuts at: 2267 2267
Size: 5846
BssSI C'ACGA_G
Cuts at: 767 4117 4475 767
Size: 3350 358 2138
Fragments arranged by size:
3350 2138 358
BstAPI GCAn_nnn'nTGC
Cuts at: 249 439 958 3223 249
Size: 190 519 2265 2872
Fragments arranged by size:
2872 2265 519 190
BstBI TT'CG_AA
Cuts at: 4207 4207
Size: 5846
BstEII G'GTnAC_C
Cuts at: 892 892
Size: 5846
BstXI CCAn_nnnn'nTGG
Cuts at: 1072 1926 2080 1072
Size: 854 154 4838
Fragments arranged by size:
4838 854 154
BstYI r'GATC_y
Cuts at: 1 2711 2871 3338 3361 3696 3942 4954 5040
Size: 2710 160 467 23 335 246 1012 86
Cuts at: 5040 5052 5398 1
Size: 12 346 449
Fragments arranged by size:
2710 1012 467 449 346 335 246 160
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1196 1286 1580 1910 1196
Size: 90 294 330 5132
Fragments arranged by size:
5132 330 294 90
BtgI C'CryG_G
Cuts at: 107 331 965 1311 1421 1433 1713 2033 2596
Size: 224 634 346 110 12 280 320 563
Cuts at: 2596 107
Size: 3357
Fragments arranged by size:
3357 634 563 346 320 280 224 110
12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 207 648 2165 3796 3977 4140 207
Size: 441 1517 1631 181 163 1913
Fragments arranged by size:
1913 1631 1517 441 181 163
BtsI GCAGTG_nn'
Cuts at: 238 433 1005 1041 3089 238
Size: 195 572 36 2048 2995
Fragments arranged by size:
2995 2048 572 195 36
ClaI AT'CG_AT
Cuts at: 536 536
Size: 5846
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1767 1781 1802 1816 1767
Size: 14 21 14 5797
Fragments arranged by size:
5797 21 14 14
DraI TTT'AAA
Cuts at: 2845 2845
Size: 5846
DrdI GACnn_nn'nnGTC
Cuts at: 351 3552 4410 351
Size: 3201 858 1787
Fragments arranged by size:
3201 1787 858
EaeI y'GGCC_r
Cuts at: 295 2030 2276 2589 3431 3605 3996 4023 5368
Size: 1735 246 313 842 174 391 27 1345
Cuts at: 5368 295
Size: 773
Fragments arranged by size:
1735 1345 842 773 391 313 246 174
27
EagI C'GGCC_G
Cuts at: 2589 3431 2589
Size: 842 5004
EarI CTCTTCn'nnn_
Cuts at: 1108 1114 1442 1469 1490 1511 3869 4079 1108
Size: 6 328 27 21 21 2358 210 2875
Fragments arranged by size:
2875 2358 328 210 27 21 21 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4054 4364 4510 5656 4054
Size: 310 146 1146 4244
Fragments arranged by size:
4244 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1563 2743 2858 1563
Size: 1180 115 4551
Fragments arranged by size:
4551 1180 115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 884 1288 1352 1948 1961 2468 3671 4103 4850
Size: 404 64 596 13 507 1203 432 747
Cuts at: 4850 5118 884
Size: 268 1612
Fragments arranged by size:
1612 1203 747 596 507 432 404 268
64 13
EcoNI CCTnn'n_nnAGG
Cuts at: 151 151
Size: 5846
EcoO109I rG'GnC_Cy
Cuts at: 629 1386 1576 1836 1905 1932 1973 2142 2703
Size: 757 190 260 69 27 41 169 561
Cuts at: 2703 2760 2836 2985 629
Size: 57 76 149 3490
Fragments arranged by size:
3490 757 561 260 190 169 149 76
69 57 41 27
EcoRI G'AATT_C
Cuts at: 2850 2850
Size: 5846
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1119 3865 1119
Size: 2746 3100
FspI TGC'GCA
Cuts at: 220 3627 220
Size: 3407 2439
FspAI rTGC'GCAy
Cuts at: 220 220
Size: 5846
HaeII r_GCGC'y
Cuts at: 263 478 1071 1636 2015 2201 2806 3280 3528
Size: 215 593 565 379 186 605 474 248
Cuts at: 3528 4550 5529 263
Size: 1022 979 580
Fragments arranged by size:
1022 979 605 593 580 565 474 379
248 215 186
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 383 415 647 679 1291 1323 1669 1701 3688
Size: 32 232 32 612 32 346 32 1987
Cuts at: 3688 3718 3720 3750 383
Size: 30 2 30 2479
Fragments arranged by size:
2479 1987 612 346 232 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2885 2885
Size: 5846
HindIII A'AGCT_T
Cuts at: 1691 1691
Size: 5846
KasI G'GCGC_C
Cuts at: 259 1632 2197 3276 3524 259
Size: 1373 565 1079 248 2581
Fragments arranged by size:
2581 1373 1079 565 248
KpnI G_GTAC'C
Cuts at: 1429 1919 2325 2866 1429
Size: 490 406 541 4409
Fragments arranged by size:
4409 541 490 406
MluI A'CGCG_T
Cuts at: 5279 5279
Size: 5846
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 713 1229 1241 2363 4517 4701 713
Size: 516 12 1122 2154 184 1858
Fragments arranged by size:
2154 1858 1122 516 184 12
MscI TGG'CCA
Cuts at: 2278 3607 2278
Size: 1329 4517
MslI CAynn'nnrTG
Cuts at: 2078 3835 3962 2078
Size: 1757 127 3962
Fragments arranged by size:
3962 1757 127
MspA1I CmG'CkG
Cuts at: 117 333 1281 2114 2670 2957 3271 3631 4644
Size: 216 948 833 556 287 314 360 1013
Cuts at: 4644 4889 117
Size: 245 1074
Fragments arranged by size:
1074 1013 948 833 556 360 314 287
245 216
NaeI GCC'GGC
Cuts at: 4027 4027
Size: 5846
NarI GG'CG_CC
Cuts at: 260 1633 2198 3277 3525 260
Size: 1373 565 1079 248 2581
Fragments arranged by size:
2581 1373 1079 565 248
NcoI C'CATG_G
Cuts at: 107 1421 1713 107
Size: 1314 292 4240
Fragments arranged by size:
4240 1314 292
NgoMIV G'CCGG_C
Cuts at: 4025 4025
Size: 5846
NheI G'CTAG_C
Cuts at: 1023 1023
Size: 5846
NspI r_CATG'y
Cuts at: 2090 2899 3399 4306 2090
Size: 809 500 907 3630
Fragments arranged by size:
3630 907 809 500
PasI CC'CwG_GG
Cuts at: 1015 1745 2419 1015
Size: 730 674 4442
Fragments arranged by size:
4442 730 674
PciI A'CATG_T
Cuts at: 4302 4302
Size: 5846
PmeI GTTT'AAAC
Cuts at: 2845 2845
Size: 5846
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4985 5017 4985
Size: 32 5814
PpuMI rG'GwC_Cy
Cuts at: 1836 1973 2142 2760 2836 1836
Size: 137 169 618 76 4846
Fragments arranged by size:
4846 618 169 137 76
PspOMI G'GGCC_C
Cuts at: 629 1575 1904 629
Size: 946 329 4571
Fragments arranged by size:
4571 946 329
PspXI vC'TCGA_Gb
Cuts at: 1841 1841
Size: 5846
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 400 432 400
Size: 32 5814
PstI C_TGCA'G
Cuts at: 165 245 1012 1280 1655 2893 3578 165
Size: 80 767 268 375 1238 685 2433
Fragments arranged by size:
2433 1238 767 685 375 268 80
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5846
PvuII CAG'CTG
Cuts at: 117 1281 3271 3631 117
Size: 1164 1990 360 2332
Fragments arranged by size:
2332 1990 1164 360
RsrII CG'GwC_CG
Cuts at: 4041 4041
Size: 5846
SacI G_AGCT'C
Cuts at: 1565 2745 2860 1565
Size: 1180 115 4551
Fragments arranged by size:
4551 1180 115
SacII CC_GC'GG
Cuts at: 334 334
Size: 5846
SalI G'TCGA_C
Cuts at: 2883 2883
Size: 5846
SanDI GG'GwC_CC
Cuts at: 1836 1836
Size: 5846
SapI GCTCTTCn'nnn_
Cuts at: 3869 4079 3869
Size: 210 5636
SbfI CC_TGCA'GG
Cuts at: 2893 2893
Size: 5846
ScaI AGT'ACT
Cuts at: 5363 5363
Size: 5846
SfcI C'TryA_G
Cuts at: 33 161 241 1008 1276 1651 2889 3574 4567
Size: 128 80 767 268 375 1238 685 993
Cuts at: 4567 4758 33
Size: 191 1121
Fragments arranged by size:
1238 1121 993 767 685 375 268 191
128 80
SfiI GGCCn_nnn'nGGCC
Cuts at: 637 637
Size: 5846
SfoI GGC'GCC
Cuts at: 261 1634 2199 3278 3526 261
Size: 1373 565 1079 248 2581
Fragments arranged by size:
2581 1373 1079 565 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5846
SmaI CCC'GGG
Cuts at: 358 804 1574 2868 358
Size: 446 770 1294 3336
Fragments arranged by size:
3336 1294 770 446
SmlI C'TyrA_G
Cuts at: 1841 2579 3001 3916 4408 4670 1841
Size: 738 422 915 492 262 3017
Fragments arranged by size:
3017 915 738 492 422 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5846
SphI G_CATG'C
Cuts at: 2899 3399 2899
Size: 500 5346
SrfI GCCC'GGGC
Cuts at: 1574 1574
Size: 5846
StuI AGG'CCT
Cuts at: 415 640 775 1201 1585 1701 415
Size: 225 135 426 384 116 4560
Fragments arranged by size:
4560 426 384 225 135 116
StyI C'CwwG_G
Cuts at: 107 696 1390 1421 1705 1713 2980 3334 107
Size: 589 694 31 284 8 1267 354 2619
Fragments arranged by size:
2619 1267 694 589 354 284 31 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 880 1074 2765 3429 3562 4021 4230 880
Size: 194 1691 664 133 459 209 2496
Fragments arranged by size:
2496 1691 664 459 209 194 133
TatI w'GTAC_w
Cuts at: 851 5361 851
Size: 4510 1336
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 804 836 2018 2050 2320 2352 5363 5395 804
Size: 32 1182 32 270 32 3011 32 1255
Fragments arranged by size:
3011 1255 1182 270 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2056 3643 2056
Size: 1587 4259
XbaI T'CTAG_A
Cuts at: 2877 2877
Size: 5846
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1072 1335 3341 1072
Size: 263 2006 3577
Fragments arranged by size:
3577 2006 263
XhoI C'TCGA_G
Cuts at: 1841 1841
Size: 5846
XmaI C'CCGG_G
Cuts at: 356 802 1572 2866 356
Size: 446 770 1294 3336
Fragments arranged by size:
3336 1294 770 446
ZraI GAC'GTC
Cuts at: 465 2773 465
Size: 2308 3538
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PasI PciI
PmeI PpiI PpuMI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AscI BbvCI BcgI BfrBI BsiWI BsmI
BsrGI BstZ17I DraIII EcoRV FseI HpaI MfeI NdeI
NotI NruI NsiI PacI PflMI PfoI PmlI PshAI
PsiI SexAI SgrAI SnaBI SspI SwaI XmnI