Restriction map for
pF1KB8567
(Circular) (Six-base) MAPSORT of: pF1KB8567.seq Check: 5498 from: 1 to: 4307
pF1KB8567 4307 bp
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AccI GT'mk_AC
Cuts at: 1345 1345
Size: 4307
Acc65I G'GTAC_C
Cuts at: 1323 1323
Size: 4307
AclI AA'CG_TT
Cuts at: 4147 4147
Size: 4307
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1249 2132 2564 2917 3579 1249
Size: 883 432 353 662 1977
Fragments arranged by size:
1977 883 662 432 353
AflII C'TTAA_G
Cuts at: 915 915
Size: 4307
AflIII A'CryG_T
Cuts at: 671 1659 3459 3740 671
Size: 988 1800 281 1238
Fragments arranged by size:
1800 1238 988 281
AlwNI CAG_nnn'CTG
Cuts at: 1039 1612 3050 1039
Size: 573 1438 2296
Fragments arranged by size:
2296 1438 573
ApaLI G'TGCA_C
Cuts at: 3145 3145
Size: 4307
ApoI r'AATT_y
Cuts at: 880 1186 1311 3660 3916 880
Size: 306 125 2349 256 1271
Fragments arranged by size:
2349 1271 306 256 125
AseI AT'TA_AT
Cuts at: 20 20
Size: 4307
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4307
AvaI C'yCGr_G
Cuts at: 1327 1327
Size: 4307
BamHI G'GATC_C
Cuts at: 1332 1332
Size: 4307
BanI G'GyrC_C
Cuts at: 1323 1737 1985 2020 1323
Size: 414 248 35 3610
Fragments arranged by size:
3610 414 248 35
BanII G_rGCy'C
Cuts at: 1321 2351 1321
Size: 1030 3277
BbeI G_GCGC'C
Cuts at: 1741 1989 1741
Size: 248 4059
BbsI GAAGACnn'nnnn_
Cuts at: 806 806
Size: 4307
BbvCI CC'TCA_GC
Cuts at: 491 1247 491
Size: 756 3551
BciVI GTATCCnnnnn_n'
Cuts at: 1102 2205 3261 4307 1102
Size: 1103 1056 1046 1102
Fragments arranged by size:
1103 1102 1056 1046
BclI T'GATC_A
Cuts at: 1827 1827
Size: 4307
BglII A'GATC_T
Cuts at: 1822 3859 1822
Size: 2037 2270
BlpI GC'TnA_GC
Cuts at: 1419 1419
Size: 4307
Bme1580I G_kGCm'C
Cuts at: 1932 2025 3149 1932
Size: 93 1124 3090
Fragments arranged by size:
3090 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1519 1600 1935 4079 1519
Size: 81 335 2144 1747
Fragments arranged by size:
2144 1747 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 417 2674 417
Size: 2257 2050
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 562
Size: 4307
Bpu10I CC'TnA_GC
Cuts at: 491 1247 491
Size: 756 3551
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1483 2362 3076 3374 1483
Size: 879 714 298 2416
Fragments arranged by size:
2416 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4011 4011
Size: 4307
BsaAI yAC'GTr
Cuts at: 1660 1660
Size: 4307
BsaBI GATnn'nnATC
Cuts at: 6 521 1821 3858 6
Size: 515 1300 2037 455
Fragments arranged by size:
2037 1300 515 455
BsaHI Gr'CG_yC
Cuts at: 123 1738 1986 2688 4184 123
Size: 1615 248 702 1496 246
Fragments arranged by size:
1615 1496 702 248 246
BsaWI w'CCGG_w
Cuts at: 1496 2017 2695 3106 3253 3527 1496
Size: 521 678 411 147 274 2276
Fragments arranged by size:
2276 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 652 682 1264 1294 652
Size: 30 582 30 3665
Fragments arranged by size:
3665 582 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 790 790
Size: 4307
BseYI C'CCAG_C
Cuts at: 1732 2490 3155 3563 1732
Size: 758 665 408 2476
Fragments arranged by size:
2476 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1849 1895 3125 104
Size: 1745 46 1230 1286
Fragments arranged by size:
1745 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1321 2099 2289 3149 1321
Size: 778 190 860 2479
Fragments arranged by size:
2479 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 1321 1932 2025 2099 2289 2351 3149 1321
Size: 611 93 74 190 62 798 2479
Fragments arranged by size:
2479 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 2739 2739
Size: 4307
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1357 1571 1873 2254 1357
Size: 214 302 381 3410
Fragments arranged by size:
3410 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2599 2653 4137 4302 50
Size: 2549 54 1484 165 55
Fragments arranged by size:
2549 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2219 2219
Size: 4307
BsrFI r'CCGG_y
Cuts at: 2305 2486 2305
Size: 181 4126
BsrGI T'GTAC_A
Cuts at: 668 668
Size: 4307
BssSI C'ACGA_G
Cuts at: 2578 3286 2578
Size: 708 3599
BstAPI GCAn_nnn'nTGC
Cuts at: 1039 1684 1039
Size: 645 3662
BstBI TT'CG_AA
Cuts at: 2668 2668
Size: 4307
BstXI CCAn_nnnn'nTGG
Cuts at: 144 615 1158 144
Size: 471 543 3293
Fragments arranged by size:
3293 543 471
BstYI r'GATC_y
Cuts at: 1 1332 1799 1822 2157 2403 2709 2721 2807
Size: 1331 467 23 335 246 306 12 86
Cuts at: 2807 3859 1
Size: 1052 449
Fragments arranged by size:
1331 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 392 392
Size: 4307
BtgI C'CryG_G
Cuts at: 107 107
Size: 4307
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1176 2257 2438 2601 1176
Size: 1081 181 163 2882
Fragments arranged by size:
2882 1081 181 163
BtsI GCAGTG_nn'
Cuts at: 507 1550 507
Size: 1043 3264
ClaI AT'CG_AT
Cuts at: 693 693
Size: 4307
DraI TTT'AAA
Cuts at: 1306 1306
Size: 4307
DrdI GACnn_nn'nnGTC
Cuts at: 2013 3357 2013
Size: 1344 2963
EaeI y'GGCC_r
Cuts at: 1148 1892 2066 2457 2484 3829 1148
Size: 744 174 391 27 1345 1626
Fragments arranged by size:
1626 1345 744 391 174 27
EagI C'GGCC_G
Cuts at: 1892 1892
Size: 4307
EarI CTCTTCn'nnn_
Cuts at: 407 576 2330 2540 407
Size: 169 1754 210 2174
Fragments arranged by size:
2174 1754 210 169
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2515 3257 3403 4117 2515
Size: 742 146 714 2705
Fragments arranged by size:
2705 742 714 146
EcoICRI GAG'CTC
Cuts at: 1319 1319
Size: 4307
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 1249 2132 2564 2917 3579 562
Size: 687 883 432 353 662 1290
Fragments arranged by size:
1290 883 687 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 376 1446 376
Size: 1070 3237
EcoRI G'AATT_C
Cuts at: 1311 1311
Size: 4307
EcoRV GAT'ATC
Cuts at: 519 519
Size: 4307
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2326 2326
Size: 4307
FspI TGC'GCA
Cuts at: 2088 2088
Size: 4307
HaeII r_GCGC'y
Cuts at: 115 1741 1989 3219 3990 115
Size: 1626 248 1230 771 432
Fragments arranged by size:
1626 1230 771 432 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 735 767 771 803 1264 1296 2149 2179 2181
Size: 32 4 32 461 32 853 30 2
Cuts at: 2181 2211 735
Size: 30 2831
Fragments arranged by size:
2831 853 461 32 32 32 30 30
4 2
HincII GTy'rAC
Cuts at: 312 1346 312
Size: 1034 3273
HindIII A'AGCT_T
Cuts at: 428 727 428
Size: 299 4008
KasI G'GCGC_C
Cuts at: 1737 1985 1737
Size: 248 4059
KpnI G_GTAC'C
Cuts at: 1327 1327
Size: 4307
MluI A'CGCG_T
Cuts at: 3740 3740
Size: 4307
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 144 3066 3250 144
Size: 2922 184 1201
Fragments arranged by size:
2922 1201 184
MscI TGG'CCA
Cuts at: 1150 2068 1150
Size: 918 3389
MslI CAynn'nnrTG
Cuts at: 613 2296 2423 613
Size: 1683 127 2497
Fragments arranged by size:
2497 1683 127
MspA1I CmG'CkG
Cuts at: 540 1039 1251 1418 1732 2092 2876 3121 540
Size: 499 212 167 314 360 784 245 1726
Fragments arranged by size:
1726 784 499 360 314 245 212 167
NaeI GCC'GGC
Cuts at: 2488 2488
Size: 4307
NarI GG'CG_CC
Cuts at: 1738 1986 1738
Size: 248 4059
NcoI C'CATG_G
Cuts at: 107 107
Size: 4307
NgoMIV G'CCGG_C
Cuts at: 2486 2486
Size: 4307
NspI r_CATG'y
Cuts at: 603 675 1360 1860 3463 603
Size: 72 685 500 1603 1447
Fragments arranged by size:
1603 1447 685 500 72
PciI A'CATG_T
Cuts at: 671 3459 671
Size: 2788 1519
PfoI T'CCnGG_A
Cuts at: 718 718
Size: 4307
PmeI GTTT'AAAC
Cuts at: 1306 1306
Size: 4307
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2753 2785 2753
Size: 32 4275
PpuMI rG'GwC_Cy
Cuts at: 376 376
Size: 4307
PstI C_TGCA'G
Cuts at: 502 659 1354 2039 502
Size: 157 695 685 2770
Fragments arranged by size:
2770 695 685 157
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4307
PvuII CAG'CTG
Cuts at: 540 1039 1251 1732 2092 540
Size: 499 212 481 360 2755
Fragments arranged by size:
2755 499 481 360 212
RsrII CG'GwC_CG
Cuts at: 2502 2502
Size: 4307
SacI G_AGCT'C
Cuts at: 1321 1321
Size: 4307
SalI G'TCGA_C
Cuts at: 1344 1344
Size: 4307
SapI GCTCTTCn'nnn_
Cuts at: 2330 2540 2330
Size: 210 4097
SbfI CC_TGCA'GG
Cuts at: 1354 1354
Size: 4307
ScaI AGT'ACT
Cuts at: 3824 3824
Size: 4307
SfcI C'TryA_G
Cuts at: 33 498 655 1350 2035 3003 3194 33
Size: 465 157 695 685 968 191 1146
Fragments arranged by size:
1146 968 695 685 465 191 157
SfoI GGC'GCC
Cuts at: 1739 1987 1739
Size: 248 4059
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4307
SmaI CCC'GGG
Cuts at: 1329 1329
Size: 4307
SmlI C'TyrA_G
Cuts at: 915 1462 2377 3091 3353 915
Size: 547 915 714 262 1869
Fragments arranged by size:
1869 915 714 547 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4307
SphI G_CATG'C
Cuts at: 603 1360 1860 603
Size: 757 500 3050
Fragments arranged by size:
3050 757 500
SspI AAT'ATT
Cuts at: 153 795 153
Size: 642 3665
StyI C'CwwG_G
Cuts at: 107 1441 1795 107
Size: 1334 354 2619
Fragments arranged by size:
2619 1334 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1890 2023 2482 2691 1890
Size: 133 459 209 3506
Fragments arranged by size:
3506 459 209 133
TatI w'GTAC_w
Cuts at: 348 668 3822 348
Size: 320 3154 833
Fragments arranged by size:
3154 833 320
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 264 296 3824 3856 264
Size: 32 3528 32 715
Fragments arranged by size:
3528 715 32 32
Tth111I GACn'n_nGTC
Cuts at: 132 2104 132
Size: 1972 2335
XbaI T'CTAG_A
Cuts at: 1338 1338
Size: 4307
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 975 1802 975
Size: 827 3480
XmaI C'CCGG_G
Cuts at: 1327 1327
Size: 4307
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BglI BmgBI
BmtI BsgI BsiWI BsmI BsmBI BspEI BssHII BstEII
BstZ17I CspCI DraIII EcoNI FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
XmnI ZraI