Restriction map for
pF1KB8568
(Circular) (Six-base) MAPSORT of: pF1KB8568.seq Check: 6517 from: 1 to: 5468
pF1KB8568 5468 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 390 2506 390
Size: 2116 3352
Acc65I G'GTAC_C
Cuts at: 2484 2484
Size: 5468
AclI AA'CG_TT
Cuts at: 5308 5308
Size: 5468
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 899 1262 1571 1667 1703 2387 2402 3293
Size: 520 363 309 96 36 684 15 891
Cuts at: 3293 3725 4078 4740 379
Size: 432 353 662 1107
Fragments arranged by size:
1107 891 684 662 520 432 363 353
309 96 36 15
AflIII A'CryG_T
Cuts at: 1420 2820 4620 4901 1420
Size: 1400 1800 281 1987
Fragments arranged by size:
1987 1800 1400 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1907 1907
Size: 5468
AlwNI CAG_nnn'CTG
Cuts at: 2773 4211 2773
Size: 1438 4030
ApaLI G'TGCA_C
Cuts at: 4306 4306
Size: 5468
ApoI r'AATT_y
Cuts at: 934 1168 1526 1941 2205 2303 2400 2472 4821
Size: 234 358 415 264 98 97 72 2349
Cuts at: 4821 5077 934
Size: 256 1325
Fragments arranged by size:
2349 1325 415 358 264 256 234 98
97 72
AseI AT'TA_AT
Cuts at: 20 2279 20
Size: 2259 3209
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5468
AvaI C'yCGr_G
Cuts at: 2488 2488
Size: 5468
BamHI G'GATC_C
Cuts at: 2493 2493
Size: 5468
BanI G'GyrC_C
Cuts at: 2484 2898 3146 3181 2484
Size: 414 248 35 4771
Fragments arranged by size:
4771 414 248 35
BanII G_rGCy'C
Cuts at: 2482 3512 2482
Size: 1030 4438
BbeI G_GCGC'C
Cuts at: 2902 3150 2902
Size: 248 5220
BbsI GAAGACnn'nnnn_
Cuts at: 296 887 1561 296
Size: 591 674 4203
Fragments arranged by size:
4203 674 591
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5434
BciVI GTATCCnnnnn_n'
Cuts at: 3366 4422 5468 3366
Size: 1056 1046 3366
Fragments arranged by size:
3366 1056 1046
BclI T'GATC_A
Cuts at: 279 2988 279
Size: 2709 2759
BfrBI ATG'CAT
Cuts at: 1857 1857
Size: 5468
BglII A'GATC_T
Cuts at: 2983 5020 2983
Size: 2037 3431
BlpI GC'TnA_GC
Cuts at: 2580 2580
Size: 5468
Bme1580I G_kGCm'C
Cuts at: 3093 3186 4310 3093
Size: 93 1124 4251
Fragments arranged by size:
4251 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 2237 2680 2761 3096 5240 2237
Size: 443 81 335 2144 2465
Fragments arranged by size:
2465 2144 443 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3835 3835
Size: 5468
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 449 568 1286 1517 449
Size: 119 718 231 4400
Fragments arranged by size:
4400 718 231 119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 2644 3523 4237 4535 218
Size: 2426 879 714 298 1151
Fragments arranged by size:
2426 1151 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5172 5172
Size: 5468
BsaAI yAC'GTr
Cuts at: 2313 2821 2313
Size: 508 4960
BsaBI GATnn'nnATC
Cuts at: 6 2982 5019 6
Size: 2976 2037 455
Fragments arranged by size:
2976 2037 455
BsaHI Gr'CG_yC
Cuts at: 2899 3147 3849 5345 2899
Size: 248 702 1496 3022
Fragments arranged by size:
3022 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2657 3178 3856 4267 4414 4688 2657
Size: 521 678 411 147 274 3437
Fragments arranged by size:
3437 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2418 2448 2418
Size: 30 5438
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1528 2008 1528
Size: 480 4988
BseYI C'CCAG_C
Cuts at: 1153 2893 3651 4316 4724 1153
Size: 1740 758 665 408 1897
Fragments arranged by size:
1897 1740 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 3010 3056 4286 104
Size: 2906 46 1230 1286
Fragments arranged by size:
2906 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1813 2482 3260 3450 4310 1813
Size: 669 778 190 860 2971
Fragments arranged by size:
2971 860 778 669 190
BsmI GAATG_Cn'
Cuts at: 873 1157 873
Size: 284 5184
Bsp1286I G_dGCh'C
Cuts at: 1813 2482 3093 3186 3260 3450 3512 4310 1813
Size: 669 611 93 74 190 62 798 2971
Fragments arranged by size:
2971 798 669 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 3900 3900
Size: 5468
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2518 2732 3034 3415 2518
Size: 214 302 381 4571
Fragments arranged by size:
4571 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3760 3814 5298 5463 50
Size: 3710 54 1484 165 55
Fragments arranged by size:
3710 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3380 3380
Size: 5468
BsrFI r'CCGG_y
Cuts at: 3466 3647 3466
Size: 181 5287
BssSI C'ACGA_G
Cuts at: 3739 4447 3739
Size: 708 4760
BstAPI GCAn_nnn'nTGC
Cuts at: 374 2845 374
Size: 2471 2997
BstBI TT'CG_AA
Cuts at: 3829 3829
Size: 5468
BstYI r'GATC_y
Cuts at: 1 2493 2960 2983 3318 3564 3870 3882 3968
Size: 2492 467 23 335 246 306 12 86
Cuts at: 3968 5020 1
Size: 1052 449
Fragments arranged by size:
2492 1052 467 449 335 306 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 391 391
Size: 5468
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3418 3599 3762 3418
Size: 181 163 5124
Fragments arranged by size:
5124 181 163
BtsI GCAGTG_nn'
Cuts at: 1889 2711 1889
Size: 822 4646
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1991 2026 1991
Size: 35 5433
DraI TTT'AAA
Cuts at: 423 888 2467 423
Size: 465 1579 3424
Fragments arranged by size:
3424 1579 465
DrdI GACnn_nn'nnGTC
Cuts at: 3174 4518 3174
Size: 1344 4124
EaeI y'GGCC_r
Cuts at: 3053 3227 3618 3645 4990 3053
Size: 174 391 27 1345 3531
Fragments arranged by size:
3531 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3053 3053
Size: 5468
EarI CTCTTCn'nnn_
Cuts at: 319 983 1238 1449 2378 3491 3701 319
Size: 664 255 211 929 1113 210 2086
Fragments arranged by size:
2086 1113 929 664 255 211 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3676 4418 4564 5278 3676
Size: 742 146 714 3866
Fragments arranged by size:
3866 742 714 146
EcoICRI GAG'CTC
Cuts at: 2480 2480
Size: 5468
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 449 568 899 1262 1286 1517 1571 1667
Size: 70 119 331 363 24 231 54 96
Cuts at: 1667 1703 2387 2402 3293 3725 4078 4740 379
Size: 36 684 15 891 432 353 662 1107
Fragments arranged by size:
1107 891 684 662 432 363 353 331
231 119 96 70 54 36 24 15
EcoO109I rG'GnC_Cy
Cuts at: 2231 2607 2231
Size: 376 5092
EcoRI G'AATT_C
Cuts at: 2400 2472 2400
Size: 72 5396
EcoRV GAT'ATC
Cuts at: 691 691
Size: 5468
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1516 3487 1516
Size: 1971 3497
FspI TGC'GCA
Cuts at: 3249 3249
Size: 5468
HaeII r_GCGC'y
Cuts at: 2902 3150 4380 5151 2902
Size: 248 1230 771 3219
Fragments arranged by size:
3219 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1752 1784 3310 3340 3342 3372 1752
Size: 32 1526 30 2 30 3848
Fragments arranged by size:
3848 1526 32 30 30 2
HincII GTy'rAC
Cuts at: 1953 2507 1953
Size: 554 4914
HindIII A'AGCT_T
Cuts at: 251 2322 251
Size: 2071 3397
KasI G'GCGC_C
Cuts at: 2898 3146 2898
Size: 248 5220
KpnI G_GTAC'C
Cuts at: 2488 2488
Size: 5468
MluI A'CGCG_T
Cuts at: 1420 4901 1420
Size: 3481 1987
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4227 4411 4227
Size: 184 5284
MscI TGG'CCA
Cuts at: 3229 3229
Size: 5468
MslI CAynn'nnrTG
Cuts at: 856 1477 3457 3584 856
Size: 621 1980 127 2740
Fragments arranged by size:
2740 1980 621 127
MspA1I CmG'CkG
Cuts at: 1407 2579 2893 3253 4037 4282 1407
Size: 1172 314 360 784 245 2593
Fragments arranged by size:
2593 1172 784 360 314 245
NaeI GCC'GGC
Cuts at: 3649 3649
Size: 5468
NarI GG'CG_CC
Cuts at: 2899 3147 2899
Size: 248 5220
NgoMIV G'CCGG_C
Cuts at: 3647 3647
Size: 5468
NsiI A_TGCA'T
Cuts at: 1859 1859
Size: 5468
NspI r_CATG'y
Cuts at: 752 2521 3021 4624 752
Size: 1769 500 1603 1596
Fragments arranged by size:
1769 1603 1596 500
PciI A'CATG_T
Cuts at: 4620 4620
Size: 5468
PflMI CCAn_nnn'nTGG
Cuts at: 984 984
Size: 5468
PfoI T'CCnGG_A
Cuts at: 1302 1302
Size: 5468
PmeI GTTT'AAAC
Cuts at: 2467 2467
Size: 5468
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3914 3946 3914
Size: 32 5436
PpuMI rG'GwC_Cy
Cuts at: 2231 2231
Size: 5468
PsiI TTA'TAA
Cuts at: 1690 2024 2453 1690
Size: 334 429 4705
Fragments arranged by size:
4705 429 334
PstI C_TGCA'G
Cuts at: 297 2515 3200 297
Size: 2218 685 2565
Fragments arranged by size:
2565 2218 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5468
PvuII CAG'CTG
Cuts at: 1407 2893 3253 1407
Size: 1486 360 3622
Fragments arranged by size:
3622 1486 360
RsrII CG'GwC_CG
Cuts at: 3663 3663
Size: 5468
SacI G_AGCT'C
Cuts at: 2482 2482
Size: 5468
SalI G'TCGA_C
Cuts at: 2505 2505
Size: 5468
SanDI GG'GwC_CC
Cuts at: 2231 2231
Size: 5468
SapI GCTCTTCn'nnn_
Cuts at: 3491 3701 3491
Size: 210 5258
SbfI CC_TGCA'GG
Cuts at: 2515 2515
Size: 5468
ScaI AGT'ACT
Cuts at: 4985 4985
Size: 5468
SfcI C'TryA_G
Cuts at: 33 293 694 2511 3196 4164 4355 33
Size: 260 401 1817 685 968 191 1146
Fragments arranged by size:
1817 1146 968 685 401 260 191
SfoI GGC'GCC
Cuts at: 2900 3148 2900
Size: 248 5220
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5468
SmaI CCC'GGG
Cuts at: 2490 2490
Size: 5468
SmlI C'TyrA_G
Cuts at: 233 2623 3538 4252 4514 233
Size: 2390 915 714 262 1187
Fragments arranged by size:
2390 1187 915 714 262
SnaBI TAC'GTA
Cuts at: 2313 2313
Size: 5468
SpeI A'CTAG_T
Cuts at: 67 2215 67
Size: 2148 3320
SphI G_CATG'C
Cuts at: 2521 3021 2521
Size: 500 4968
SspI AAT'ATT
Cuts at: 450 1618 2283 450
Size: 1168 665 3635
Fragments arranged by size:
3635 1168 665
StyI C'CwwG_G
Cuts at: 640 968 1200 2602 2956 640
Size: 328 232 1402 354 3152
Fragments arranged by size:
3152 1402 354 328 232
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3051 3184 3643 3852 3051
Size: 133 459 209 4667
Fragments arranged by size:
4667 459 209 133
TatI w'GTAC_w
Cuts at: 1485 2182 4983 1485
Size: 697 2801 1970
Fragments arranged by size:
2801 1970 697
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1931 1963 4985 5017 1931
Size: 32 3022 32 2382
Fragments arranged by size:
3022 2382 32 32
Tth111I GACn'n_nGTC
Cuts at: 3265 3265
Size: 5468
XbaI T'CTAG_A
Cuts at: 2499 2499
Size: 5468
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2963 2963
Size: 5468
XmaI C'CCGG_G
Cuts at: 2488 2488
Size: 5468
XmnI GAAnn'nnTTC
Cuts at: 1463 1463
Size: 5468
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BstZ17I BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NsiI NspI PciI PflMI PfoI PmeI PpiI PpuMI
PsiI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
ApaI AscI AvrII BaeI BbvCI BglI BmgBI BmtI
Bpu10I BsgI BsiWI BsmBI BspEI BsrGI BssHII BstEII
BstXI Bsu36I BtgI ClaI DraIII EcoNI FseI FspAI
HpaI MfeI NcoI NdeI NheI NotI NruI PacI
PasI PmlI PshAI PspOMI PspXI PsrI SacII SexAI
SfiI SgrAI SrfI StuI SwaI XhoI ZraI