Restriction map for
pF1KB8573
(Circular) (Six-base) MAPSORT of: pF1KB8573.seq Check: 8062 from: 1 to: 5783
pF1KB8573 5783 bp
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AccI GT'mk_AC
Cuts at: 308 842 2821 308
Size: 534 1979 3270
Fragments arranged by size:
3270 1979 534
Acc65I G'GTAC_C
Cuts at: 622 862 2599 2799 622
Size: 240 1737 200 3606
Fragments arranged by size:
3606 1737 240 200
AclI AA'CG_TT
Cuts at: 5623 5623
Size: 5783
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 320 448 730 1264 1678 2158 2249 2335 3608
Size: 128 282 534 414 480 91 86 1273
Cuts at: 3608 4040 4393 5055 320
Size: 432 353 662 1048
Fragments arranged by size:
1273 1048 662 534 480 432 414 353
282 128 91 86
AfeI AGC'GCT
Cuts at: 1348 1348
Size: 5783
AflIII A'CryG_T
Cuts at: 3135 4935 5216 3135
Size: 1800 281 3702
Fragments arranged by size:
3702 1800 281
AgeI A'CCGG_T
Cuts at: 1457 1457
Size: 5783
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 238 238
Size: 5783
AlwNI CAG_nnn'CTG
Cuts at: 970 1473 2116 2155 2472 3088 4526 970
Size: 503 643 39 317 616 1438 2227
Fragments arranged by size:
2227 1438 643 616 503 317 39
ApaLI G'TGCA_C
Cuts at: 4621 4621
Size: 5783
ApoI r'AATT_y
Cuts at: 1880 2661 2787 5136 5392 1880
Size: 781 126 2349 256 2271
Fragments arranged by size:
2349 2271 781 256 126
AseI AT'TA_AT
Cuts at: 20 20
Size: 5783
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5783
AvaI C'yCGr_G
Cuts at: 1502 2803 1502
Size: 1301 4482
BamHI G'GATC_C
Cuts at: 119 1268 2720 2808 119
Size: 1149 1452 88 3094
Fragments arranged by size:
3094 1452 1149 88
BanI G'GyrC_C
Cuts at: 622 862 2599 2799 3213 3461 3496 622
Size: 240 1737 200 414 248 35 2909
Fragments arranged by size:
2909 1737 414 248 240 200 35
BanII G_rGCy'C
Cuts at: 899 1796 2797 3827 899
Size: 897 1001 1030 2855
Fragments arranged by size:
2855 1030 1001 897
BbeI G_GCGC'C
Cuts at: 3217 3465 3217
Size: 248 5535
BbsI GAAGACnn'nnnn_
Cuts at: 1684 1825 1684
Size: 141 5642
BbvCI CC'TCA_GC
Cuts at: 1163 2271 1163
Size: 1108 4675
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 858 892 858
Size: 34 5749
BciVI GTATCCnnnnn_n'
Cuts at: 1710 3681 4737 5783 1710
Size: 1971 1056 1046 1710
Fragments arranged by size:
1971 1710 1056 1046
BclI T'GATC_A
Cuts at: 3303 3303
Size: 5783
BfrBI ATG'CAT
Cuts at: 1866 1866
Size: 5783
BglI GCCn_nnn'nGGC
Cuts at: 683 683
Size: 5783
BglII A'GATC_T
Cuts at: 482 1028 1134 3298 5335 482
Size: 546 106 2164 2037 930
Fragments arranged by size:
2164 2037 930 546 106
BlpI GC'TnA_GC
Cuts at: 2540 2895 2540
Size: 355 5428
Bme1580I G_kGCm'C
Cuts at: 960 3408 3501 4625 960
Size: 2448 93 1124 2118
Fragments arranged by size:
2448 2118 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1660 2414 2995 3076 3411 5555 1660
Size: 754 581 81 335 2144 1888
Fragments arranged by size:
2144 1888 754 581 335 81
BmtI G_CTAG'C
Cuts at: 1622 1622
Size: 5783
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 661 2235 4150 661
Size: 1574 1915 2294
Fragments arranged by size:
2294 1915 1574
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1221 1879 1221
Size: 658 5125
Bpu10I CC'TnA_GC
Cuts at: 1163 1724 2271 1163
Size: 561 547 4675
Fragments arranged by size:
4675 561 547
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 333 1978 2959 3838 4552 4850 333
Size: 1645 981 879 714 298 1266
Fragments arranged by size:
1645 1266 981 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 382 5487 382
Size: 5105 678
BsaAI yAC'GTr
Cuts at: 3136 3136
Size: 5783
BsaBI GATnn'nnATC
Cuts at: 6 1659 3297 5334 6
Size: 1653 1638 2037 455
Fragments arranged by size:
2037 1653 1638 455
BsaHI Gr'CG_yC
Cuts at: 3214 3462 4164 5660 3214
Size: 248 702 1496 3337
Fragments arranged by size:
3337 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1457 2972 3493 4171 4582 4729 5003 1457
Size: 1515 521 678 411 147 274 2237
Fragments arranged by size:
2237 1515 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 802 832 1219 1249 1283 1313 1954 1984 802
Size: 30 387 30 34 30 641 30 4601
Fragments arranged by size:
4601 641 387 34 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 823 826 849 1180 1804 823
Size: 3 23 331 624 4802
Fragments arranged by size:
4802 624 331 23 3
BseYI C'CCAG_C
Cuts at: 892 1705 2165 3208 3966 4631 5039 892
Size: 813 460 1043 758 665 408 1636
Fragments arranged by size:
1636 1043 813 758 665 460 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1651 1947 1651
Size: 296 5487
BsiEI CG_ry'CG
Cuts at: 104 3325 3371 4601 104
Size: 3221 46 1230 1286
Fragments arranged by size:
3221 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1796 2797 3575 3765 4625 1796
Size: 1001 778 190 860 2954
Fragments arranged by size:
2954 1001 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 249 249
Size: 5783
Bsp1286I G_dGCh'C
Cuts at: 899 960 1796 2797 3408 3501 3575 3765 3827
Size: 61 836 1001 611 93 74 190 62
Cuts at: 3827 4625 899
Size: 798 2057
Fragments arranged by size:
2057 1001 836 798 611 190 93 74
62 61
BspHI T'CATG_A
Cuts at: 4215 4215
Size: 5783
BspMI ACCTGCnnnn'nnnn_
Cuts at: 230 261 2692 2833 3047 3349 3730 230
Size: 31 2431 141 214 302 381 2283
Fragments arranged by size:
2431 2283 381 302 214 141 31
BsrBI CCG'CTC
Cuts at: 50 2037 4075 4129 5613 5778 50
Size: 1987 2038 54 1484 165 55
Fragments arranged by size:
2038 1987 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 441 887 1613 1893 3695 441
Size: 446 726 280 1802 2529
Fragments arranged by size:
2529 1802 726 446 280
BsrFI r'CCGG_y
Cuts at: 1457 3781 3962 1457
Size: 2324 181 3278
Fragments arranged by size:
3278 2324 181
BssSI C'ACGA_G
Cuts at: 4054 4762 4054
Size: 708 5075
BstAPI GCAn_nnn'nTGC
Cuts at: 1092 2155 2536 3160 1092
Size: 1063 381 624 3715
Fragments arranged by size:
3715 1063 624 381
BstBI TT'CG_AA
Cuts at: 2658 4144 2658
Size: 1486 4297
BstXI CCAn_nnnn'nTGG
Cuts at: 487 2295 2404 487
Size: 1808 109 3866
Fragments arranged by size:
3866 1808 109
BstYI r'GATC_y
Cuts at: 1 119 482 755 1013 1028 1134 1268 2720
Size: 118 363 273 258 15 106 134 1452
Cuts at: 2720 2808 3275 3298 3633 3879 4185 4197 4283
Size: 88 467 23 335 246 306 12 86
Cuts at: 4283 5335 1
Size: 1052 449
Fragments arranged by size:
1452 1052 467 449 363 335 306 273
258 246 134 118 106 88 86 23
15 12
BstZ17I GTA'TAC
Cuts at: 309 309
Size: 5783
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3733 3914 4077 3733
Size: 181 163 5439
Fragments arranged by size:
5439 181 163
BtsI GCAGTG_nn'
Cuts at: 968 1822 1935 2206 3026 968
Size: 854 113 271 820 3725
Fragments arranged by size:
3725 854 820 271 113
ClaI AT'CG_AT
Cuts at: 951 951
Size: 5783
DraI TTT'AAA
Cuts at: 2782 2782
Size: 5783
DraIII CAC_nnn'GTG
Cuts at: 1327 1327
Size: 5783
DrdI GACnn_nn'nnGTC
Cuts at: 238 3489 4833 238
Size: 3251 1344 1188
Fragments arranged by size:
3251 1344 1188
EaeI y'GGCC_r
Cuts at: 450 1598 3368 3542 3933 3960 5305 450
Size: 1148 1770 174 391 27 1345 928
Fragments arranged by size:
1770 1345 1148 928 391 174 27
EagI C'GGCC_G
Cuts at: 3368 3368
Size: 5783
EarI CTCTTCn'nnn_
Cuts at: 424 826 906 1357 1595 2018 2048 2225 2306
Size: 402 80 451 238 423 30 177 81
Cuts at: 2306 3806 4016 424
Size: 1500 210 2191
Fragments arranged by size:
2191 1500 451 423 402 238 210 177
81 80 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 119 3991 4733 4879 5593 119
Size: 3872 742 146 714 309
Fragments arranged by size:
3872 742 714 309 146
EcoICRI GAG'CTC
Cuts at: 1794 2795 1794
Size: 1001 4782
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 320 448 730 1221 1264 1678 1879 2158 2249
Size: 128 282 491 43 414 201 279 91
Cuts at: 2249 2335 3608 4040 4393 5055 320
Size: 86 1273 432 353 662 1048
Fragments arranged by size:
1273 1048 662 491 432 414 353 282
279 201 128 91 86 43
EcoNI CCTnn'n_nnAGG
Cuts at: 805 831 805
Size: 26 5757
EcoO109I rG'GnC_Cy
Cuts at: 323 2922 323
Size: 2599 3184
EcoRI G'AATT_C
Cuts at: 2787 2787
Size: 5783
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 139 706 3802 139
Size: 567 3096 2120
Fragments arranged by size:
3096 2120 567
FspI TGC'GCA
Cuts at: 3564 3564
Size: 5783
HaeII r_GCGC'y
Cuts at: 664 1350 2595 3217 3465 4695 5466 664
Size: 686 1245 622 248 1230 771 981
Fragments arranged by size:
1245 1230 981 771 686 622 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 111 143 187 219 802 834 1283 1315 3625
Size: 32 44 32 583 32 449 32 2310
Cuts at: 3625 3655 3657 3687 111
Size: 30 2 30 2207
Fragments arranged by size:
2310 2207 583 449 44 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1499 2822 1499
Size: 1323 4460
KasI G'GCGC_C
Cuts at: 3213 3461 3213
Size: 248 5535
KpnI G_GTAC'C
Cuts at: 626 866 2603 2803 626
Size: 240 1737 200 3606
Fragments arranged by size:
3606 1737 240 200
MluI A'CGCG_T
Cuts at: 5216 5216
Size: 5783
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 323 721 783 835 4542 4726 323
Size: 398 62 52 3707 184 1380
Fragments arranged by size:
3707 1380 398 184 62 52
MscI TGG'CCA
Cuts at: 452 1600 3544 452
Size: 1148 1944 2691
Fragments arranged by size:
2691 1944 1148
MslI CAynn'nnrTG
Cuts at: 1774 2293 3772 3899 1774
Size: 519 1479 127 3658
Fragments arranged by size:
3658 1479 519 127
MspA1I CmG'CkG
Cuts at: 136 190 933 1424 1857 1914 2894 3208 3568
Size: 54 743 491 433 57 980 314 360
Cuts at: 3568 4352 4597 136
Size: 784 245 1322
Fragments arranged by size:
1322 980 784 743 491 433 360 314
245 57 54
NaeI GCC'GGC
Cuts at: 3964 3964
Size: 5783
NarI GG'CG_CC
Cuts at: 3214 3462 3214
Size: 248 5535
NdeI CA'TA_TG
Cuts at: 582 582
Size: 5783
NgoMIV G'CCGG_C
Cuts at: 3962 3962
Size: 5783
NheI G'CTAG_C
Cuts at: 1618 1618
Size: 5783
NsiI A_TGCA'T
Cuts at: 1868 1868
Size: 5783
NspI r_CATG'y
Cuts at: 1636 2534 2836 3336 4939 1636
Size: 898 302 500 1603 2480
Fragments arranged by size:
2480 1603 898 500 302
PasI CC'CwG_GG
Cuts at: 1416 1416
Size: 5783
PciI A'CATG_T
Cuts at: 4935 4935
Size: 5783
PflMI CCAn_nnn'nTGG
Cuts at: 2472 2472
Size: 5783
PmeI GTTT'AAAC
Cuts at: 2782 2782
Size: 5783
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4229 4261 4229
Size: 32 5751
PshAI GACnn'nnGTC
Cuts at: 2088 2088
Size: 5783
PspXI vC'TCGA_Gb
Cuts at: 1502 1502
Size: 5783
PstI C_TGCA'G
Cuts at: 195 2486 2707 2830 3515 195
Size: 2291 221 123 685 2463
Fragments arranged by size:
2463 2291 685 221 123
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5783
PvuII CAG'CTG
Cuts at: 190 933 1857 3208 3568 190
Size: 743 924 1351 360 2405
Fragments arranged by size:
2405 1351 924 743 360
RsrII CG'GwC_CG
Cuts at: 3978 3978
Size: 5783
SacI G_AGCT'C
Cuts at: 1796 2797 1796
Size: 1001 4782
SalI G'TCGA_C
Cuts at: 2820 2820
Size: 5783
SapI GCTCTTCn'nnn_
Cuts at: 424 2048 3806 4016 424
Size: 1624 1758 210 2191
Fragments arranged by size:
2191 1758 1624 210
SbfI CC_TGCA'GG
Cuts at: 2830 2830
Size: 5783
ScaI AGT'ACT
Cuts at: 5300 5300
Size: 5783
SexAI A'CCwGG_T
Cuts at: 1472 1472
Size: 5783
SfcI C'TryA_G
Cuts at: 33 191 1852 2482 2703 2826 3511 4479 4670
Size: 158 1661 630 221 123 685 968 191
Cuts at: 4670 33
Size: 1146
Fragments arranged by size:
1661 1146 968 685 630 221 191 158
123
SfoI GGC'GCC
Cuts at: 3215 3463 3215
Size: 248 5535
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5783
SmaI CCC'GGG
Cuts at: 2805 2805
Size: 5783
SmlI C'TyrA_G
Cuts at: 312 1502 1993 2938 3853 4567 4829 312
Size: 1190 491 945 915 714 262 1266
Fragments arranged by size:
1266 1190 945 915 714 491 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5783
SphI G_CATG'C
Cuts at: 1636 2836 3336 1636
Size: 1200 500 4083
Fragments arranged by size:
4083 1200 500
StuI AGG'CCT
Cuts at: 800 1171 1739 800
Size: 371 568 4844
Fragments arranged by size:
4844 568 371
StyI C'CwwG_G
Cuts at: 410 681 1512 2568 2595 2917 3271 410
Size: 271 831 1056 27 322 354 2922
Fragments arranged by size:
2922 1056 831 354 322 271 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 423 1234 1960 2179 3366 3499 3958 4167 423
Size: 811 726 219 1187 133 459 209 2039
Fragments arranged by size:
2039 1187 811 726 459 219 209 133
TatI w'GTAC_w
Cuts at: 549 5298 549
Size: 4749 1034
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 968 1000 1909 1941 5300 5332 968
Size: 32 909 32 3359 32 1419
Fragments arranged by size:
3359 1419 909 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 157 3580 157
Size: 3423 2360
XbaI T'CTAG_A
Cuts at: 2814 2814
Size: 5783
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3278 3278
Size: 5783
XhoI C'TCGA_G
Cuts at: 1502 1502
Size: 5783
XmaI C'CCGG_G
Cuts at: 2803 2803
Size: 5783
XmnI GAAnn'nnTTC
Cuts at: 1589 2027 2650 1589
Size: 438 623 4722
Fragments arranged by size:
4722 623 438
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BstZ17I BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NheI NsiI NspI PasI PciI PflMI PmeI
PpiI PshAI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BmgBI BsiWI BsmI BspEI BsrGI BssHII
BstEII Bsu36I BtgI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NotI NruI PacI PfoI PmlI
PpuMI PsiI PspOMI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI SwaI ZraI