Restriction map for
pF1KB8574
(Circular) (Six-base) MAPSORT of: pF1KB8574.seq Check: 8002 from: 1 to: 5456
pF1KB8574 5456 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 903 1902 2494 903
Size: 999 592 3865
Fragments arranged by size:
3865 999 592
Acc65I G'GTAC_C
Cuts at: 2472 2472
Size: 5456
AclI AA'CG_TT
Cuts at: 5296 5296
Size: 5456
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 935 1504 1700 1960 2121 3281 3713 4066 4728
Size: 569 196 260 161 1160 432 353 662
Cuts at: 4728 935
Size: 1663
Fragments arranged by size:
1663 1160 662 569 432 353 260 196
161
AflIII A'CryG_T
Cuts at: 2808 4608 4889 2808
Size: 1800 281 3375
Fragments arranged by size:
3375 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1898 1898
Size: 5456
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1810 1810
Size: 5456
AlwNI CAG_nnn'CTG
Cuts at: 208 2116 2238 2761 4199 208
Size: 1908 122 523 1438 1465
Fragments arranged by size:
1908 1465 1438 523 122
ApaI G_GGCC'C
Cuts at: 2154 2290 2154
Size: 136 5320
ApaLI G'TGCA_C
Cuts at: 4294 4294
Size: 5456
ApoI r'AATT_y
Cuts at: 538 1111 1165 2439 2445 2460 4809 5065 538
Size: 573 54 1274 6 15 2349 256 929
Fragments arranged by size:
2349 1274 929 573 256 54 15 6
AseI AT'TA_AT
Cuts at: 20 20
Size: 5456
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5456
AvaI C'yCGr_G
Cuts at: 1606 2476 1606
Size: 870 4586
BamHI G'GATC_C
Cuts at: 930 2481 930
Size: 1551 3905
BanI G'GyrC_C
Cuts at: 2262 2472 2886 3134 3169 2262
Size: 210 414 248 35 4549
Fragments arranged by size:
4549 414 248 210 35
BanII G_rGCy'C
Cuts at: 1703 2154 2252 2290 2470 3500 1703
Size: 451 98 38 180 1030 3659
Fragments arranged by size:
3659 1030 451 180 98 38
BbeI G_GCGC'C
Cuts at: 2890 3138 2890
Size: 248 5208
BbsI GAAGACnn'nnnn_
Cuts at: 127 1399 127
Size: 1272 4184
BciVI GTATCCnnnnn_n'
Cuts at: 3354 4410 5456 3354
Size: 1056 1046 3354
Fragments arranged by size:
3354 1056 1046
BclI T'GATC_A
Cuts at: 351 2976 351
Size: 2625 2831
BglI GCCn_nnn'nGGC
Cuts at: 2200 2200
Size: 5456
BglII A'GATC_T
Cuts at: 2971 5008 2971
Size: 2037 3419
BlpI GC'TnA_GC
Cuts at: 2568 2568
Size: 5456
Bme1580I G_kGCm'C
Cuts at: 2154 2290 3081 3174 4298 2154
Size: 136 791 93 1124 3312
Fragments arranged by size:
3312 1124 791 136 93
BmrI ACTGGGnnnn_n'
Cuts at: 210 230 2668 2749 3084 5228 210
Size: 20 2438 81 335 2144 438
Fragments arranged by size:
2438 2144 438 335 81 20
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3823 3823
Size: 5456
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1285 1401 1687 2239 2416 1285
Size: 116 286 552 177 4325
Fragments arranged by size:
4325 552 286 177 116
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1005 2632 3511 4225 4523 1005
Size: 1627 879 714 298 1938
Fragments arranged by size:
1938 1627 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5160 5160
Size: 5456
BsaAI yAC'GTr
Cuts at: 2809 2809
Size: 5456
BsaBI GATnn'nnATC
Cuts at: 6 2970 5007 6
Size: 2964 2037 455
Fragments arranged by size:
2964 2037 455
BsaHI Gr'CG_yC
Cuts at: 2887 3135 3837 5333 2887
Size: 248 702 1496 3010
Fragments arranged by size:
3010 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2645 3166 3844 4255 4402 4676 2645
Size: 521 678 411 147 274 3425
Fragments arranged by size:
3425 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 735 765 735
Size: 30 5426
BseYI C'CCAG_C
Cuts at: 140 2269 2290 2316 2881 3639 4304 4712 140
Size: 2129 21 26 565 758 665 408 884
Fragments arranged by size:
2129 884 758 665 565 408 26 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 593 1252 2248 593
Size: 659 996 3801
Fragments arranged by size:
3801 996 659
BsiEI CG_ry'CG
Cuts at: 104 2998 3044 4274 104
Size: 2894 46 1230 1286
Fragments arranged by size:
2894 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1703 2470 3248 3438 4298 1703
Size: 767 778 190 860 2861
Fragments arranged by size:
2861 860 778 767 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1531 1531
Size: 5456
Bsp1286I G_dGCh'C
Cuts at: 1703 2154 2252 2290 2470 3081 3174 3248 3438
Size: 451 98 38 180 611 93 74 190
Cuts at: 3438 3500 4298 1703
Size: 62 798 2861
Fragments arranged by size:
2861 798 611 451 190 180 98 93
74 62 38
BspHI T'CATG_A
Cuts at: 3888 3888
Size: 5456
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2506 2720 3022 3403 2506
Size: 214 302 381 4559
Fragments arranged by size:
4559 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3748 3802 5286 5451 50
Size: 3698 54 1484 165 55
Fragments arranged by size:
3698 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 500 869 3368 500
Size: 369 2499 2588
Fragments arranged by size:
2588 2499 369
BsrFI r'CCGG_y
Cuts at: 3454 3635 3454
Size: 181 5275
BsrGI T'GTAC_A
Cuts at: 760 760
Size: 5456
BssSI C'ACGA_G
Cuts at: 3727 4435 3727
Size: 708 4748
BstAPI GCAn_nnn'nTGC
Cuts at: 2833 2833
Size: 5456
BstBI TT'CG_AA
Cuts at: 3817 3817
Size: 5456
BstEII G'GTnAC_C
Cuts at: 466 466
Size: 5456
BstXI CCAn_nnnn'nTGG
Cuts at: 1311 1311
Size: 5456
BstYI r'GATC_y
Cuts at: 1 885 930 1454 1757 1875 2132 2481 2948
Size: 884 45 524 303 118 257 349 467
Cuts at: 2948 2971 3306 3552 3858 3870 3956 5008 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 884 524 467 449 349 335 306
303 257 246 118 86 45 23 12
BstZ17I GTA'TAC
Cuts at: 904 904
Size: 5456
Bsu36I CC'TnA_GG
Cuts at: 2258 2258
Size: 5456
BtgI C'CryG_G
Cuts at: 107 107
Size: 5456
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3406 3587 3750 3406
Size: 181 163 5112
Fragments arranged by size:
5112 181 163
BtsI GCAGTG_nn'
Cuts at: 683 1337 2699 683
Size: 654 1362 3440
Fragments arranged by size:
3440 1362 654
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 592 627 592
Size: 35 5421
DraI TTT'AAA
Cuts at: 1200 2455 1200
Size: 1255 4201
DrdI GACnn_nn'nnGTC
Cuts at: 3162 4506 3162
Size: 1344 4112
EaeI y'GGCC_r
Cuts at: 3041 3215 3606 3633 4978 3041
Size: 174 391 27 1345 3519
Fragments arranged by size:
3519 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3041 3041
Size: 5456
EarI CTCTTCn'nnn_
Cuts at: 653 1137 1210 1676 2330 3479 3689 653
Size: 484 73 466 654 1149 210 2420
Fragments arranged by size:
2420 1149 654 484 466 210 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3664 4406 4552 5266 3664
Size: 742 146 714 3854
Fragments arranged by size:
3854 742 714 146
EcoICRI GAG'CTC
Cuts at: 1701 2468 1701
Size: 767 4689
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 935 1285 1401 1504 1687 1700 1960 2121 2239
Size: 350 116 103 183 13 260 161 118
Cuts at: 2239 2416 3281 3713 4066 4728 935
Size: 177 865 432 353 662 1663
Fragments arranged by size:
1663 865 662 432 353 350 260 183
177 161 118 116 103 13
EcoO109I rG'GnC_Cy
Cuts at: 616 2150 2286 2287 2595 616
Size: 1534 136 1 308 3477
Fragments arranged by size:
3477 1534 308 136 1
EcoRI G'AATT_C
Cuts at: 2460 2460
Size: 5456
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3475 3475
Size: 5456
FspI TGC'GCA
Cuts at: 3237 3237
Size: 5456
HaeII r_GCGC'y
Cuts at: 1424 2890 3138 4368 5139 1424
Size: 1466 248 1230 771 1741
Fragments arranged by size:
1741 1466 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 391 423 475 507 1641 1660 1673 1692 1884
Size: 32 52 32 1134 19 13 19 192
Cuts at: 1884 1916 3298 3328 3330 3360 391
Size: 32 1382 30 2 30 2487
Fragments arranged by size:
2487 1382 1134 192 52 32 32 32
30 30 19 19 13 2
HincII GTy'rAC
Cuts at: 1903 2495 1903
Size: 592 4864
HindIII A'AGCT_T
Cuts at: 917 917
Size: 5456
KasI G'GCGC_C
Cuts at: 2886 3134 2886
Size: 248 5208
KpnI G_GTAC'C
Cuts at: 2476 2476
Size: 5456
MluI A'CGCG_T
Cuts at: 4889 4889
Size: 5456
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1217 1305 4215 4399 1217
Size: 88 2910 184 2274
Fragments arranged by size:
2910 2274 184 88
MscI TGG'CCA
Cuts at: 3217 3217
Size: 5456
MslI CAynn'nnrTG
Cuts at: 272 3445 3572 272
Size: 3173 127 2156
Fragments arranged by size:
3173 2156 127
MspA1I CmG'CkG
Cuts at: 673 2294 2567 2881 3241 4025 4270 673
Size: 1621 273 314 360 784 245 1859
Fragments arranged by size:
1859 1621 784 360 314 273 245
NaeI GCC'GGC
Cuts at: 3637 3637
Size: 5456
NarI GG'CG_CC
Cuts at: 2887 3135 2887
Size: 248 5208
NcoI C'CATG_G
Cuts at: 107 107
Size: 5456
NgoMIV G'CCGG_C
Cuts at: 3635 3635
Size: 5456
NspI r_CATG'y
Cuts at: 132 287 1254 1291 2509 3009 4612 132
Size: 155 967 37 1218 500 1603 976
Fragments arranged by size:
1603 1218 976 967 500 155 37
PciI A'CATG_T
Cuts at: 4608 4608
Size: 5456
PflMI CCAn_nnn'nTGG
Cuts at: 2172 2172
Size: 5456
PmeI GTTT'AAAC
Cuts at: 2455 2455
Size: 5456
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3902 3934 3902
Size: 32 5424
PpuMI rG'GwC_Cy
Cuts at: 616 616
Size: 5456
PsiI TTA'TAA
Cuts at: 227 227
Size: 5456
PspOMI G'GGCC_C
Cuts at: 2150 2286 2150
Size: 136 5320
PstI C_TGCA'G
Cuts at: 151 678 1789 2304 2503 3188 151
Size: 527 1111 515 199 685 2419
Fragments arranged by size:
2419 1111 685 527 515 199
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5456
PvuII CAG'CTG
Cuts at: 673 2294 2881 3241 673
Size: 1621 587 360 2888
Fragments arranged by size:
2888 1621 587 360
RsrII CG'GwC_CG
Cuts at: 3651 3651
Size: 5456
SacI G_AGCT'C
Cuts at: 1703 2470 1703
Size: 767 4689
SalI G'TCGA_C
Cuts at: 1901 2493 1901
Size: 592 4864
SapI GCTCTTCn'nnn_
Cuts at: 1210 1676 2330 3479 3689 1210
Size: 466 654 1149 210 2977
Fragments arranged by size:
2977 1149 654 466 210
SbfI CC_TGCA'GG
Cuts at: 2503 2503
Size: 5456
ScaI AGT'ACT
Cuts at: 4973 4973
Size: 5456
SfcI C'TryA_G
Cuts at: 33 147 674 815 1785 2300 2499 3184 4152
Size: 114 527 141 970 515 199 685 968
Cuts at: 4152 4343 33
Size: 191 1146
Fragments arranged by size:
1146 970 968 685 527 515 199 191
141 114
SfoI GGC'GCC
Cuts at: 2888 3136 2888
Size: 248 5208
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5456
SmaI CCC'GGG
Cuts at: 2478 2478
Size: 5456
SmlI C'TyrA_G
Cuts at: 1020 2611 3526 4240 4502 1020
Size: 1591 915 714 262 1974
Fragments arranged by size:
1974 1591 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5456
SphI G_CATG'C
Cuts at: 1254 1291 2509 3009 1254
Size: 37 1218 500 3701
Fragments arranged by size:
3701 1218 500 37
StuI AGG'CCT
Cuts at: 1720 2256 1720
Size: 536 4920
StyI C'CwwG_G
Cuts at: 107 1090 1409 1460 2411 2590 2944 107
Size: 983 319 51 951 179 354 2619
Fragments arranged by size:
2619 983 951 354 319 179 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3039 3172 3631 3840 3039
Size: 133 459 209 4655
Fragments arranged by size:
4655 459 209 133
TatI w'GTAC_w
Cuts at: 760 4971 760
Size: 4211 1245
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 145 177 4973 5005 145
Size: 32 4796 32 596
Fragments arranged by size:
4796 596 32 32
Tth111I GACn'n_nGTC
Cuts at: 3253 3253
Size: 5456
XbaI T'CTAG_A
Cuts at: 2487 2487
Size: 5456
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2951 2951
Size: 5456
XmaI C'CCGG_G
Cuts at: 2476 2476
Size: 5456
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PpiI PpuMI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BseRI BsiWI BsmI BspEI BssHII ClaI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PshAI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI