Restriction map for
pF1KB8577
(Circular) (Six-base) MAPSORT of: pF1KB8577.seq Check: 7776 from: 1 to: 5675
pF1KB8577 5675 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AatII G_ACGT'C
Cuts at: 155 155
Size: 5675
AccI GT'mk_AC
Cuts at: 2713 2713
Size: 5675
Acc65I G'GTAC_C
Cuts at: 2691 2691
Size: 5675
AclI AA'CG_TT
Cuts at: 5515 5515
Size: 5675
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 299 509 3500 3932 4285 4947 299
Size: 210 2991 432 353 662 1027
Fragments arranged by size:
2991 1027 662 432 353 210
AflIII A'CryG_T
Cuts at: 270 1653 3027 4827 5108 270
Size: 1383 1374 1800 281 837
Fragments arranged by size:
1800 1383 1374 837 281
AleI CACnn'nnGTG
Cuts at: 870 870
Size: 5675
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1056 1088 1595 1627 1056
Size: 32 507 32 5104
Fragments arranged by size:
5104 507 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2980 4418 2980
Size: 1438 4237
ApaLI G'TGCA_C
Cuts at: 4513 4513
Size: 5675
ApoI r'AATT_y
Cuts at: 134 511 2679 5028 5284 134
Size: 377 2168 2349 256 525
Fragments arranged by size:
2349 2168 525 377 256
AseI AT'TA_AT
Cuts at: 20 2603 20
Size: 2583 3092
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5675
AvaI C'yCGr_G
Cuts at: 2695 2695
Size: 5675
BamHI G'GATC_C
Cuts at: 1610 2700 1610
Size: 1090 4585
BanI G'GyrC_C
Cuts at: 2691 3105 3353 3388 2691
Size: 414 248 35 4978
Fragments arranged by size:
4978 414 248 35
BanII G_rGCy'C
Cuts at: 1773 2689 3719 1773
Size: 916 1030 3729
Fragments arranged by size:
3729 1030 916
BbeI G_GCGC'C
Cuts at: 3109 3357 3109
Size: 248 5427
BbsI GAAGACnn'nnnn_
Cuts at: 2582 2582
Size: 5675
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1163 1197 1163
Size: 34 5641
BciVI GTATCCnnnnn_n'
Cuts at: 267 1507 1803 3573 4629 5675 267
Size: 1240 296 1770 1056 1046 267
Fragments arranged by size:
1770 1240 1056 1046 296 267
BclI T'GATC_A
Cuts at: 2486 3195 2486
Size: 709 4966
BfrBI ATG'CAT
Cuts at: 1553 2100 2468 1553
Size: 547 368 4760
Fragments arranged by size:
4760 547 368
BglII A'GATC_T
Cuts at: 1782 3190 5227 1782
Size: 1408 2037 2230
Fragments arranged by size:
2230 2037 1408
BlpI GC'TnA_GC
Cuts at: 1393 2787 1393
Size: 1394 4281
Bme1580I G_kGCm'C
Cuts at: 782 1416 3300 3393 4517 782
Size: 634 1884 93 1124 1940
Fragments arranged by size:
1940 1884 1124 634 93
BmrI ACTGGGnnnn_n'
Cuts at: 769 2887 2968 3303 5447 769
Size: 2118 81 335 2144 997
Fragments arranged by size:
2144 2118 997 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1490 4042 1490
Size: 2552 3123
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1298 1392 1787 1298
Size: 94 395 5186
Fragments arranged by size:
5186 395 94
Bpu10I CC'TnA_GC
Cuts at: 1678 1772 1678
Size: 94 5581
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2851 3730 4444 4742 2851
Size: 879 714 298 3784
Fragments arranged by size:
3784 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1559 5379 1559
Size: 3820 1855
BsaAI yAC'GTr
Cuts at: 3028 3028
Size: 5675
BsaBI GATnn'nnATC
Cuts at: 6 656 3189 5226 6
Size: 650 2533 2037 455
Fragments arranged by size:
2533 2037 650 455
BsaHI Gr'CG_yC
Cuts at: 152 3106 3354 4056 5552 152
Size: 2954 248 702 1496 275
Fragments arranged by size:
2954 1496 702 275 248
BsaWI w'CCGG_w
Cuts at: 2864 3385 4063 4474 4621 4895 2864
Size: 521 678 411 147 274 3644
Fragments arranged by size:
3644 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 199 229 515 545 1024 1054 1056 1086 1760
Size: 30 286 30 479 30 2 30 674
Cuts at: 1760 1790 2206 2236 199
Size: 30 416 30 3638
Fragments arranged by size:
3638 674 479 416 286 30 30 30
30 30 30 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 703 703
Size: 5675
BseYI C'CCAG_C
Cuts at: 3100 3858 4523 4931 3100
Size: 758 665 408 3844
Fragments arranged by size:
3844 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 889 1603 2488 2535 535
Size: 354 714 885 47 3675
Fragments arranged by size:
3675 885 714 354 47
BsiEI CG_ry'CG
Cuts at: 104 3217 3263 4493 104
Size: 3113 46 1230 1286
Fragments arranged by size:
3113 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1298 1773 2689 3467 3657 4517 1298
Size: 475 916 778 190 860 2456
Fragments arranged by size:
2456 916 860 778 475 190
BsmI GAATG_Cn'
Cuts at: 1237 1553 2621 1237
Size: 316 1068 4291
Fragments arranged by size:
4291 1068 316
Bsp1286I G_dGCh'C
Cuts at: 782 1298 1416 1773 2689 3300 3393 3467 3657
Size: 516 118 357 916 611 93 74 190
Cuts at: 3657 3719 4517 782
Size: 62 798 1940
Fragments arranged by size:
1940 916 798 611 516 357 190 118
93 74 62
BspHI T'CATG_A
Cuts at: 4107 4107
Size: 5675
BspMI ACCTGCnnnn'nnnn_
Cuts at: 959 1322 2510 2725 2939 3241 3622 959
Size: 363 1188 215 214 302 381 3012
Fragments arranged by size:
3012 1188 381 363 302 215 214
BsrBI CCG'CTC
Cuts at: 50 1133 3967 4021 5505 5670 50
Size: 1083 2834 54 1484 165 55
Fragments arranged by size:
2834 1484 1083 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2038 2288 3587 2038
Size: 250 1299 4126
Fragments arranged by size:
4126 1299 250
BsrFI r'CCGG_y
Cuts at: 3673 3854 3673
Size: 181 5494
BssSI C'ACGA_G
Cuts at: 3946 4654 3946
Size: 708 4967
BstAPI GCAn_nnn'nTGC
Cuts at: 1056 3052 1056
Size: 1996 3679
BstBI TT'CG_AA
Cuts at: 717 4036 717
Size: 3319 2356
BstYI r'GATC_y
Cuts at: 1 1281 1610 1782 1863 2633 2700 3167 3190
Size: 1280 329 172 81 770 67 467 23
Cuts at: 3190 3525 3771 4077 4089 4175 5227 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1280 1052 770 467 449 335 329 306
246 172 86 81 67 23 12
Bsu36I CC'TnA_GG
Cuts at: 1482 2230 1482
Size: 748 4927
BtgI C'CryG_G
Cuts at: 107 1569 2274 107
Size: 1462 705 3508
Fragments arranged by size:
3508 1462 705
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3625 3806 3969 3625
Size: 181 163 5331
Fragments arranged by size:
5331 181 163
BtsI GCAGTG_nn'
Cuts at: 877 2918 877
Size: 2041 3634
ClaI AT'CG_AT
Cuts at: 668 668
Size: 5675
DraI TTT'AAA
Cuts at: 767 2674 767
Size: 1907 3768
DraIII CAC_nnn'GTG
Cuts at: 1291 1291
Size: 5675
DrdI GACnn_nn'nnGTC
Cuts at: 3381 4725 3381
Size: 1344 4331
EaeI y'GGCC_r
Cuts at: 1128 2580 3260 3434 3825 3852 5197 1128
Size: 1452 680 174 391 27 1345 1606
Fragments arranged by size:
1606 1452 1345 680 391 174 27
EagI C'GGCC_G
Cuts at: 3260 3260
Size: 5675
EarI CTCTTCn'nnn_
Cuts at: 3698 3908 3698
Size: 210 5465
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1171 3883 4625 4771 5485 1171
Size: 2712 742 146 714 1361
Fragments arranged by size:
2712 1361 742 714 146
EcoICRI GAG'CTC
Cuts at: 1771 2687 1771
Size: 916 4759
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 299 509 1298 1392 1787 3500 3932 4285 4947
Size: 210 789 94 395 1713 432 353 662
Cuts at: 4947 299
Size: 1027
Fragments arranged by size:
1713 1027 789 662 432 395 353 210
94
EcoO109I rG'GnC_Cy
Cuts at: 1121 2007 2814 1121
Size: 886 807 3982
Fragments arranged by size:
3982 886 807
EcoRI G'AATT_C
Cuts at: 134 2679 134
Size: 2545 3130
EcoRV GAT'ATC
Cuts at: 147 147
Size: 5675
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1352 3694 1352
Size: 2342 3333
FspI TGC'GCA
Cuts at: 967 3456 967
Size: 2489 3186
HaeII r_GCGC'y
Cuts at: 3109 3357 4587 5358 3109
Size: 248 1230 771 3426
Fragments arranged by size:
3426 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1024 1056 2223 2255 2287 3517 3547 3549 3579
Size: 32 1167 32 32 1230 30 2 30
Cuts at: 3579 1024
Size: 3120
Fragments arranged by size:
3120 1230 1167 32 32 32 30 30
2
HincII GTy'rAC
Cuts at: 2079 2714 2079
Size: 635 5040
HpaI GTT'AAC
Cuts at: 2079 2079
Size: 5675
KasI G'GCGC_C
Cuts at: 3105 3353 3105
Size: 248 5427
KpnI G_GTAC'C
Cuts at: 2695 2695
Size: 5675
MfeI C'AATT_G
Cuts at: 897 1589 2086 897
Size: 692 497 4486
Fragments arranged by size:
4486 692 497
MluI A'CGCG_T
Cuts at: 5108 5108
Size: 5675
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1793 4434 4618 1793
Size: 2641 184 2850
Fragments arranged by size:
2850 2641 184
MscI TGG'CCA
Cuts at: 3436 3436
Size: 5675
MslI CAynn'nnrTG
Cuts at: 112 668 870 2510 3664 3791 112
Size: 556 202 1640 1154 127 1996
Fragments arranged by size:
1996 1640 1154 556 202 127
MspA1I CmG'CkG
Cuts at: 837 1216 1608 2786 3100 3460 4244 4489 837
Size: 379 392 1178 314 360 784 245 2023
Fragments arranged by size:
2023 1178 784 392 379 360 314 245
NaeI GCC'GGC
Cuts at: 3856 3856
Size: 5675
NarI GG'CG_CC
Cuts at: 3106 3354 3106
Size: 248 5427
NcoI C'CATG_G
Cuts at: 107 2274 107
Size: 2167 3508
NdeI CA'TA_TG
Cuts at: 300 300
Size: 5675
NgoMIV G'CCGG_C
Cuts at: 3854 3854
Size: 5675
NsiI A_TGCA'T
Cuts at: 1555 2102 2470 1555
Size: 547 368 4760
Fragments arranged by size:
4760 547 368
NspI r_CATG'y
Cuts at: 274 613 1657 2728 3228 4831 274
Size: 339 1044 1071 500 1603 1118
Fragments arranged by size:
1603 1118 1071 1044 500 339
PasI CC'CwG_GG
Cuts at: 1151 1728 1151
Size: 577 5098
PciI A'CATG_T
Cuts at: 270 1653 4827 270
Size: 1383 3174 1118
Fragments arranged by size:
3174 1383 1118
PmeI GTTT'AAAC
Cuts at: 2674 2674
Size: 5675
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1056 1088 4121 4153 1056
Size: 32 3033 32 2578
Fragments arranged by size:
3033 2578 32 32
PpuMI rG'GwC_Cy
Cuts at: 1121 2007 1121
Size: 886 4789
PstI C_TGCA'G
Cuts at: 1215 2722 3407 1215
Size: 1507 685 3483
Fragments arranged by size:
3483 1507 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5675
PvuII CAG'CTG
Cuts at: 837 1216 3100 3460 837
Size: 379 1884 360 3052
Fragments arranged by size:
3052 1884 379 360
RsrII CG'GwC_CG
Cuts at: 3870 3870
Size: 5675
SacI G_AGCT'C
Cuts at: 1773 2689 1773
Size: 916 4759
SalI G'TCGA_C
Cuts at: 2712 2712
Size: 5675
SapI GCTCTTCn'nnn_
Cuts at: 3698 3908 3698
Size: 210 5465
SbfI CC_TGCA'GG
Cuts at: 2722 2722
Size: 5675
ScaI AGT'ACT
Cuts at: 5192 5192
Size: 5675
SfcI C'TryA_G
Cuts at: 33 341 1211 2718 3403 4371 4562 33
Size: 308 870 1507 685 968 191 1146
Fragments arranged by size:
1507 1146 968 870 685 308 191
SfoI GGC'GCC
Cuts at: 3107 3355 3107
Size: 248 5427
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5675
SmaI CCC'GGG
Cuts at: 2697 2697
Size: 5675
SmlI C'TyrA_G
Cuts at: 2830 3745 4459 4721 2830
Size: 915 714 262 3784
Fragments arranged by size:
3784 915 714 262
SpeI A'CTAG_T
Cuts at: 67 1788 67
Size: 1721 3954
SphI G_CATG'C
Cuts at: 2728 3228 2728
Size: 500 5175
SspI AAT'ATT
Cuts at: 1630 2601 1630
Size: 971 4704
StuI AGG'CCT
Cuts at: 1683 1683
Size: 5675
StyI C'CwwG_G
Cuts at: 107 2274 2369 2809 3163 107
Size: 2167 95 440 354 2619
Fragments arranged by size:
2619 2167 440 354 95
SwaI ATTT'AAAT
Cuts at: 767 767
Size: 5675
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 575 3258 3391 3850 4059 575
Size: 2683 133 459 209 2191
Fragments arranged by size:
2683 2191 459 209 133
TatI w'GTAC_w
Cuts at: 267 469 5190 267
Size: 202 4721 752
Fragments arranged by size:
4721 752 202
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 515 547 1760 1792 2099 2131 5192 5224 515
Size: 32 1213 32 307 32 3061 32 966
Fragments arranged by size:
3061 1213 966 307 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2545 3472 2545
Size: 927 4748
XbaI T'CTAG_A
Cuts at: 2706 2706
Size: 5675
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3170 3170
Size: 5675
XmaI C'CCGG_G
Cuts at: 2695 2695
Size: 5675
XmnI GAAnn'nnTTC
Cuts at: 713 713
Size: 5675
ZraI GAC'GTC
Cuts at: 153 153
Size: 5675
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PasI PciI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AlfI ApaI AscI
AvrII BaeI BbvCI BglI BmgBI BmtI BsiWI BsmBI
BspEI BsrGI BssHII BstEII BstXI BstZ17I CspCI EcoNI
FseI FspAI HindIII NheI NotI NruI PacI PflMI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI XhoI