Restriction map for
pF1KB8580
(Circular) (Six-base) MAPSORT of: pF1KB8580.seq Check: 225 from: 1 to: 4559
pF1KB8580 4559 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1461 1461
Size: 4559
AccI GT'mk_AC
Cuts at: 822 1597 822
Size: 775 3784
Acc65I G'GTAC_C
Cuts at: 1575 1575
Size: 4559
AclI AA'CG_TT
Cuts at: 4399 4399
Size: 4559
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 2384 2816 3169 3831 292
Size: 2092 432 353 662 1020
Fragments arranged by size:
2092 1020 662 432 353
AfeI AGC'GCT
Cuts at: 280 280
Size: 4559
AflIII A'CryG_T
Cuts at: 1911 3711 3992 1911
Size: 1800 281 2478
Fragments arranged by size:
2478 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 973 1864 3302 973
Size: 891 1438 2230
Fragments arranged by size:
2230 1438 891
ApaI G_GGCC'C
Cuts at: 172 215 367 172
Size: 43 152 4364
Fragments arranged by size:
4364 152 43
ApaLI G'TGCA_C
Cuts at: 3397 3397
Size: 4559
ApoI r'AATT_y
Cuts at: 613 958 986 1529 1563 3912 4168 613
Size: 345 28 543 34 2349 256 1004
Fragments arranged by size:
2349 1004 543 345 256 34 28
AseI AT'TA_AT
Cuts at: 20 20
Size: 4559
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4559
AvaI C'yCGr_G
Cuts at: 118 1046 1579 118
Size: 928 533 3098
Fragments arranged by size:
3098 928 533
BamHI G'GATC_C
Cuts at: 1584 1584
Size: 4559
BanI G'GyrC_C
Cuts at: 346 1575 1989 2237 2272 346
Size: 1229 414 248 35 2633
Fragments arranged by size:
2633 1229 414 248 35
BanII G_rGCy'C
Cuts at: 172 215 367 485 1573 2603 172
Size: 43 152 118 1088 1030 2128
Fragments arranged by size:
2128 1088 1030 152 118 43
BbeI G_GCGC'C
Cuts at: 350 1993 2241 350
Size: 1643 248 2668
Fragments arranged by size:
2668 1643 248
BbvCI CC'TCA_GC
Cuts at: 155 686 155
Size: 531 4028
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1055 1089 1055
Size: 34 4525
BciVI GTATCCnnnnn_n'
Cuts at: 2457 3513 4559 2457
Size: 1056 1046 2457
Fragments arranged by size:
2457 1056 1046
BclI T'GATC_A
Cuts at: 2079 2079
Size: 4559
BglI GCCn_nnn'nGGC
Cuts at: 496 496
Size: 4559
BglII A'GATC_T
Cuts at: 2074 4111 2074
Size: 2037 2522
BlpI GC'TnA_GC
Cuts at: 310 1671 310
Size: 1361 3198
Bme1580I G_kGCm'C
Cuts at: 172 215 367 2184 2277 3401 172
Size: 43 152 1817 93 1124 1330
Fragments arranged by size:
1817 1330 1124 152 93 43
BmgBI CAC'GTC
Cuts at: 452 477 452
Size: 25 4534
BmrI ACTGGGnnnn_n'
Cuts at: 340 1771 1852 2187 4331 340
Size: 1431 81 335 2144 568
Fragments arranged by size:
2144 1431 568 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 820 2926 820
Size: 2106 2453
Bpu10I CC'TnA_GC
Cuts at: 155 686 155
Size: 531 4028
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1735 2614 3328 3626 1735
Size: 879 714 298 2668
Fragments arranged by size:
2668 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4263 4263
Size: 4559
BsaAI yAC'GTr
Cuts at: 1912 1912
Size: 4559
BsaBI GATnn'nnATC
Cuts at: 6 2073 4110 6
Size: 2067 2037 455
Fragments arranged by size:
2067 2037 455
BsaHI Gr'CG_yC
Cuts at: 145 265 347 422 1990 2238 2940 4436 145
Size: 120 82 75 1568 248 702 1496 268
Fragments arranged by size:
1568 1496 702 268 248 120 82 75
BsaWI w'CCGG_w
Cuts at: 932 1748 2269 2947 3358 3505 3779 932
Size: 816 521 678 411 147 274 1712
Fragments arranged by size:
1712 816 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 130 160 824 854 130
Size: 30 664 30 3835
Fragments arranged by size:
3835 664 30 30
BseYI C'CCAG_C
Cuts at: 133 358 588 845 1130 1984 2742 3407 3815
Size: 225 230 257 285 854 758 665 408
Cuts at: 3815 133
Size: 877
Fragments arranged by size:
877 854 758 665 408 285 257 230
225
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 764 803 764
Size: 39 4520
BsiEI CG_ry'CG
Cuts at: 104 404 458 2101 2147 3377 104
Size: 300 54 1643 46 1230 1286
Fragments arranged by size:
1643 1286 1230 300 54 46
BsiHKAI G_wGCw'C
Cuts at: 1573 2351 2541 3401 1573
Size: 778 190 860 2731
Fragments arranged by size:
2731 860 778 190
BsmI GAATG_Cn'
Cuts at: 1099 1099
Size: 4559
Bsp1286I G_dGCh'C
Cuts at: 172 215 367 485 1573 2184 2277 2351 2541
Size: 43 152 118 1088 611 93 74 190
Cuts at: 2541 2603 3401 172
Size: 62 798 1330
Fragments arranged by size:
1330 1088 798 611 190 152 118 93
74 62 43
BspHI T'CATG_A
Cuts at: 2991 2991
Size: 4559
BspMI ACCTGCnnnn'nnnn_
Cuts at: 958 1461 1609 1823 2125 2506 958
Size: 503 148 214 302 381 3011
Fragments arranged by size:
3011 503 381 302 214 148
BsrBI CCG'CTC
Cuts at: 50 2851 2905 4389 4554 50
Size: 2801 54 1484 165 55
Fragments arranged by size:
2801 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2471 2471
Size: 4559
BsrFI r'CCGG_y
Cuts at: 403 1294 2557 2738 403
Size: 891 1263 181 2224
Fragments arranged by size:
2224 1263 891 181
BssSI C'ACGA_G
Cuts at: 2830 3538 2830
Size: 708 3851
BstAPI GCAn_nnn'nTGC
Cuts at: 643 742 973 1936 643
Size: 99 231 963 3266
Fragments arranged by size:
3266 963 231 99
BstBI TT'CG_AA
Cuts at: 2920 2920
Size: 4559
BstYI r'GATC_y
Cuts at: 1 1181 1210 1355 1584 2051 2074 2409 2655
Size: 1180 29 145 229 467 23 335 246
Cuts at: 2655 2961 2973 3059 4111 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1180 1052 467 449 335 306 246 229
145 86 29 23 12
BtgI C'CryG_G
Cuts at: 107 173 107
Size: 66 4493
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 329 2509 2690 2853 329
Size: 2180 181 163 2035
Fragments arranged by size:
2180 2035 181 163
BtsI GCAGTG_nn'
Cuts at: 1802 1802
Size: 4559
DraI TTT'AAA
Cuts at: 1558 1558
Size: 4559
DraIII CAC_nnn'GTG
Cuts at: 607 781 607
Size: 174 4385
DrdI GACnn_nn'nnGTC
Cuts at: 264 2265 3609 264
Size: 2001 1344 1214
Fragments arranged by size:
2001 1344 1214
EaeI y'GGCC_r
Cuts at: 401 443 455 2144 2318 2709 2736 4081 401
Size: 42 12 1689 174 391 27 1345 879
Fragments arranged by size:
1689 1345 879 391 174 42 27 12
EagI C'GGCC_G
Cuts at: 401 455 2144 401
Size: 54 1689 2816
Fragments arranged by size:
2816 1689 54
EarI CTCTTCn'nnn_
Cuts at: 298 2582 2792 298
Size: 2284 210 2065
Fragments arranged by size:
2284 2065 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 175 2767 3509 3655 4369 175
Size: 2592 742 146 714 365
Fragments arranged by size:
2592 742 714 365 146
EcoICRI GAG'CTC
Cuts at: 1571 1571
Size: 4559
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 2384 2816 3169 3831 292
Size: 2092 432 353 662 1020
Fragments arranged by size:
2092 1020 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 169 211 212 363 970 1698 169
Size: 42 1 151 607 728 3030
Fragments arranged by size:
3030 728 607 151 42 1
EcoRI G'AATT_C
Cuts at: 1563 1563
Size: 4559
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 297 2578 297
Size: 2281 2278
FspI TGC'GCA
Cuts at: 2340 2340
Size: 4559
HaeII r_GCGC'y
Cuts at: 282 350 413 502 1993 2241 3471 4242 282
Size: 68 63 89 1491 248 1230 771 599
Fragments arranged by size:
1491 1230 771 599 248 89 68 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 130 162 1064 1096 1137 1169 2401 2431 2433
Size: 32 902 32 41 32 1232 30 2
Cuts at: 2433 2463 130
Size: 30 2226
Fragments arranged by size:
2226 1232 902 41 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 823 1035 1598 823
Size: 212 563 3784
Fragments arranged by size:
3784 563 212
KasI G'GCGC_C
Cuts at: 346 1989 2237 346
Size: 1643 248 2668
Fragments arranged by size:
2668 1643 248
KpnI G_GTAC'C
Cuts at: 1579 1579
Size: 4559
MfeI C'AATT_G
Cuts at: 950 950
Size: 4559
MluI A'CGCG_T
Cuts at: 3992 3992
Size: 4559
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1173 3318 3502 1173
Size: 2145 184 2230
Fragments arranged by size:
2230 2145 184
MscI TGG'CCA
Cuts at: 445 2320 445
Size: 1875 2684
MslI CAynn'nnrTG
Cuts at: 2548 2675 2548
Size: 127 4432
MspA1I CmG'CkG
Cuts at: 133 175 231 358 1130 1670 1984 2344 3128
Size: 42 56 127 772 540 314 360 784
Cuts at: 3128 3373 133
Size: 245 1319
Fragments arranged by size:
1319 784 772 540 360 314 245 127
56 42
NaeI GCC'GGC
Cuts at: 405 2740 405
Size: 2335 2224
NarI GG'CG_CC
Cuts at: 347 1990 2238 347
Size: 1643 248 2668
Fragments arranged by size:
2668 1643 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4559
NgoMIV G'CCGG_C
Cuts at: 403 2738 403
Size: 2335 2224
NspI r_CATG'y
Cuts at: 1385 1612 2112 3715 1385
Size: 227 500 1603 2229
Fragments arranged by size:
2229 1603 500 227
PasI CC'CwG_GG
Cuts at: 1278 1278
Size: 4559
PciI A'CATG_T
Cuts at: 3711 3711
Size: 4559
PmeI GTTT'AAAC
Cuts at: 1558 1558
Size: 4559
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3005 3037 3005
Size: 32 4527
PpuMI rG'GwC_Cy
Cuts at: 970 970
Size: 4559
PspOMI G'GGCC_C
Cuts at: 168 211 363 168
Size: 43 152 4364
Fragments arranged by size:
4364 152 43
PstI C_TGCA'G
Cuts at: 230 1606 2291 230
Size: 1376 685 2498
Fragments arranged by size:
2498 1376 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4559
PvuII CAG'CTG
Cuts at: 133 1984 2344 133
Size: 1851 360 2348
Fragments arranged by size:
2348 1851 360
RsrII CG'GwC_CG
Cuts at: 2754 2754
Size: 4559
SacI G_AGCT'C
Cuts at: 1573 1573
Size: 4559
SacII CC_GC'GG
Cuts at: 176 176
Size: 4559
SalI G'TCGA_C
Cuts at: 821 1596 821
Size: 775 3784
SapI GCTCTTCn'nnn_
Cuts at: 2582 2792 2582
Size: 210 4349
SbfI CC_TGCA'GG
Cuts at: 1606 1606
Size: 4559
ScaI AGT'ACT
Cuts at: 4076 4076
Size: 4559
SfcI C'TryA_G
Cuts at: 33 226 1187 1226 1602 2287 3255 3446 33
Size: 193 961 39 376 685 968 191 1146
Fragments arranged by size:
1146 968 961 685 376 193 191 39
SfoI GGC'GCC
Cuts at: 348 1991 2239 348
Size: 1643 248 2668
Fragments arranged by size:
2668 1643 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4559
SmaI CCC'GGG
Cuts at: 1581 1581
Size: 4559
SmlI C'TyrA_G
Cuts at: 1714 2629 3343 3605 1714
Size: 915 714 262 2668
Fragments arranged by size:
2668 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4559
SphI G_CATG'C
Cuts at: 1612 2112 1612
Size: 500 4059
StyI C'CwwG_G
Cuts at: 107 260 593 1693 2047 107
Size: 153 333 1100 354 2619
Fragments arranged by size:
2619 1100 354 333 153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1079 2142 2275 2734 2943 1079
Size: 1063 133 459 209 2695
Fragments arranged by size:
2695 1063 459 209 133
TatI w'GTAC_w
Cuts at: 4074 4074
Size: 4559
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4076 4108 4076
Size: 32 4527
Tth111I GACn'n_nGTC
Cuts at: 2356 2356
Size: 4559
XbaI T'CTAG_A
Cuts at: 705 1590 705
Size: 885 3674
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2054 2054
Size: 4559
XmaI C'CCGG_G
Cuts at: 1579 1579
Size: 4559
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AgeI AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BfrBI BmtI BpmI BseRI BsiWI
BsmBI BspEI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
NdeI NheI NotI NruI NsiI PacI PflMI PfoI
PmlI PshAI PsiI PspXI PsrI SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI